Abstract
IN 1926, Knaffl-Lenz1 introduced a method for the bioassay of digitalis preparations by determining the minimal lethal dose on intravenous injection in anæsthetized guinea pigs. The method, which may be used for all kinds of cardiac glucosides, has since become official in, for example, Britain2 and Sweden3. According to this procedure, the solution to be tested is introduced into the vena jugularis under constant pressure and at constant speed (either from a burette with a Mariotte stopper or with a motor-driven injection apparatus). The lethal dose is determined by direct observation of the cardiac standstill (concerning the details of the procedure, see, for example, Burn4, Fønss Bech5 and Goldberg6). The number of animals required is determined by the degree of accuracy one wishes to give the results ; this may be calculated with the help of the usual statistical methods.
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References
Knaffl-Lenz, E., J. Pharm. Exp. Ther., 29, 407 (1926).
"Brit. Pharm.", 623 (1932).
Pharm. Suec., 11, 788 (1946).
Burn, J. H., "Biologische Auswertungsmethoden", 180 (Berlin, 1937).
Fønss Bech, P., Dansk Tidskr. Farm., 16, 57 (1942).
Goldberg, L., Acta Physiol. Scand., 4, 178 (1942).
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WIEDLING, S. A Simple Method for Direct Comparison of the Depressant Effect of the Barbiturates on the Respiration and Circulation. Nature 162, 1003 (1948). https://doi.org/10.1038/1621003a0
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DOI: https://doi.org/10.1038/1621003a0
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