Abstract
FEW attempts have been made to maintain (or grow) animal tissue cells in protein-free media, although it has been shown1 that the low molecular weight substances of blood plasma are of the utmost importance for the life of tissue cells, which rapidly perish in dialysed blood plasma. It has further been possible1,2, by the addition of a number of aminoacids together with glutamine, glutathione and hexose diphosphate to a medium containing only dialysed plasma proteins and dialysed embryonic extract, to obtain survival and growth of the cells. The few attempts made to devise completely synthetic media have met with only partial success because of lack of a suitable technique, and because very little is really known about the fundamental nutritional requirements of animal tissue cells during survival and growth.
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References
Fischer, A., Acta physiol. Scand., 2, 143 (1941).
Fischer, A., Astrup, T., Ehrensvärd, G., and Oehlensehlager, Proc. Soc. Exp. Biol. Med. (in the press).
Lewis, M. R., and Lewis, W. H., Anat. Rec., 5, 277 (1911).
White, P. R., Growth, 10, 231 (1946).
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JENSEN, A. Animal Tissue Cells in Protein-Free Media. Nature 161, 273 (1948). https://doi.org/10.1038/161273a0
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DOI: https://doi.org/10.1038/161273a0
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