Abstract
Adenomatous polyposis coli (APC) is a multifunctional tumour suppressor protein, central to development and the mature organism. It is mutated in most cases of colorectal cancer, rendering it ineffective in mediating β-catenin degradation. We show that localization of full-length APC in colon carcinoma and noncancer cell lines is independent of cell density. However, the location of truncated APC is a function of cell density and in high-density cells truncated APC is predominantly not nuclear. Although the distribution of truncated APC and β-catenin is closely linked in subconfluent SW480 cells, at high cell density they are not colocalized. We postulated that in this cell line this could be due to an increase in β-catenin bound to E-cadherin with formation of adherens junctions at high cell density. However, while in coimmunoprecipitation assays we observe an increase in binding between β-catenin and E-cadherin and a corresponding decrease in binding between β-catenin and APC at high cell density, we did not observe a strict colocalization of β-catenin and E-cadherin at the membrane of all cells.
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References
Aberle H, Bauer A, Stappert J, Kispert A and Kemler R . (1997). EMBO J., 16, 3797–3804.
Allan V and Nathke IS . (2001). Nat. Cell Biol., 3, E226–E228.
Behrens J, Mareel MM, Van Roy FM and Birchmeier W . (1989). J. Cell. Biol., 108, 2435–2447.
Behrens J, von Kries JP, Kuhl M, Bruhn L, Wedlich D, Grosschedl R and Birchmeier W . (1996). Nature, 382, 638–642.
Bienz M . (2002). Nat. Rev. Mol. Cell. Biol., 3, 328–338.
Brabletz T, Jung A, Reu S, Porzner M, Hlubek F, Kunz-Schugart L.A, Knuechel R and Kirchner T . (2001). Proc. Natl. Acad. Sci. USA, 98, 10356–10361.
Brocardo MG, Bianchini M, Radrizzani M, Reyes GB, Dugour AV, Taminelli GL, Gonzalez Solveyra C and Santa-Coloma TA . (2001). Biochem. Biophys. Res. Comm., 284, 982–986.
Crawford HC, Fingleton BM, Rudolph-Owen LA, Goss KJ, Rubinfeld B, Polakis P and Matrisian LM . (1999). Oncogene, 18, 2883–2891.
Dietrich C, Scherwat J, Faust D and Oesch F . (2002). Biochem. Biophys. Res. Comm., 292, 195–199.
Eleftheriou A, Yoshida M and Henderson BR . (2001). J. Biol. Chem., 276, 25883–25888.
Fearon ER and Vogelstein B . (1990). Cell, 61, 759–767.
Fornerod M, Ohno M, Yoshida M and Mattaj IW . (1997). Cell, 90, 1051–1060.
Gorlich D . (1998). EMBO J., 17, 2721–2727.
Gottardi CJ, Wong E and Gumbiner BM . (2001). J. Cell. Biol., 153, 1049–1059.
Groden J, Thliveris A, Samowitz W, Carlson M, Gelbert L, Albertsen H, Joslyn G, Stevens J, Spirio L, Robertson M, Sargeant L, Krapcho K, Wolff E, Burt R, Hughes JP, Warrington J, McPherson J, Wasmuth J, LePaslier D, Abderrahim H, Cohen D, Leppert M and White R . (1991). Cell, 66, 589–600.
He TC, Sparks AB, Rago C, Hermeking H, Zawel L, da Costa LT, Morin PJ, Vogelstein B and Kinzler KW . (1998). Science, 281, 1509–1512.
Henderson BR . (2000). Nat. Cell Biol., 2, 653–660.
Henderson BR and Fagotto F . (2002). EMBO Rep., 3, 834–839.
Hulsken J, Birchmeier W and Behrens J . (1994). J. Cell. Biol., 127, 2061–2069.
Joslyn G, Carlson M, Thliveris A, Albertsen H, Gelbert L, Samowitz W, Groden J, Stevens J, Spirio L, Robertson M, Sargeant L, Krapcho E, Wolff E, Burt R, Hughes JP, Warrington J, McPherson J, Wasmuth J, LePaslier D, Abderrahim H, Cohen D, Leppert M and White R . (1991). Cell, 66, 601–613.
Kinzler KW, Nilbert MC, Su L-K, Vogelstein B, Bryan TM, Levy DB, Smith KJ, Preisinger AC, Hedge P, McKechnie D, Finniear R, Markham A, Groffen J, Boguski MS, Altschul SF, Horii A, Ando Y, Miyoshi Y, Miki Y, Nishisho I and Nakamura Y . (1991). Science, 253, 661–664.
Nathke IS, Adams CL, Polakis P, Sellin JH and Nelson J . (1996). J. Cell. Biol., 134, 165–179.
Neufeld KL, Zhang F, Cullen BR and White RL . (2000). EMBO Rep., 6, 519–523.
Nishisho I, Nakamura Y, Miyoshi Y, Miki Y, Ando H, Horii A, Koyama K, Utsunomiya J, Baba S, Hedge P, Markham A, Krush AJ, Petersen G, Hamilton SR, Nilbert MC, Levy D, Bryan TM, Preisinger AC, Smith KJ, Su L-K, Kinzler K and Vogelstein B . (1991). Science, 253, 665–668.
Orsulic S, Huber O, Aberle H, Arnold S and Kemler R . (1999). J. Cell Sci., 112, 1237–1245.
Perl AK, Wilgenbus P, Dahl U, Semb H and Christofori G . (1998). Nature, 392, 190–193.
Powel SM, Zilz N, Beazer-Barclay Y, Bryan TM, Hamilton SR, Thibodeau SN, Vogelstein B and Kinzler KW . (1992). Nature, 359, 235–237.
Roose J, Huls G, vanBeest M, Moerer P, van der Horn K, Goldscheding R, Logtenberg T and Clevers H . (1999). Science, 285, 1923–1926.
Rosin-Arbesfeld R, Townsley F and Bienz M . (2000). Nature, 406, 1009–1012.
Rosin-Arbesfeld R, Ihrke G and Bienz M . (2001). EMBO J., 20, 5929–5939.
Rosin-Arbesfeld R, Cliffe A, Brabletz T and Bienz M . (2003). EMBO J., 22, 1101–1113.
Rubinfeld B, Souza B, Albert I, Munemitsu S and Polakis P . (1995). J. Biol. Chem., 270, 5549–5555.
Sadot E, Simcha I, Shtutman M, Ben-Ze'er A and Geiger B . (1998). Proc. Natl. Acad. Sci. USA, 95, 15339–15344.
Shtutman M, Shurinsky J, Simcha I, Albanese C, D'Amico M, Pestell R and Ben-Ze'er A . (1999). Proc. Natl. Acad. Sci. USA, 96, 5522–5527.
Tetsu O and McCormick F . (1999). Nature, 398, 422–426.
Wieschens N and Fagotto F . (2001). Curr. Biol., 11, 18–27.
Zhang F, White RL and Neufeld KL . (2001). Mol. Cell. Biol., 21, 8143–8156.
Acknowledgements
We thank Dr IS Näthke, University of Dundee for the kind gift of antibody to APC (M-APC). Melanie L Davies is funded through Tenovus Cancer Research Campaign grant 35141; Gwyndaf T Roberts was supported by BBSRC grant 35086. J Wakeman was supported by a Royal Society award and North West Cancer Research Fund. Thanks to Alysia Battersby, David Pryce and Ramsay McFarlane for critical reading of this manuscript.
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Davies, M., Roberts, G., Spiller, D. et al. Density-dependent location and interactions of truncated APC and β-catenin. Oncogene 23, 1412–1419 (2004). https://doi.org/10.1038/sj.onc.1207266
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DOI: https://doi.org/10.1038/sj.onc.1207266
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