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The C-terminal region of cellular Qin oligomerizes: correlation with oncogenic transformation and transcriptional repression

Oncogene volume 22, pages 1908–1915 (2003)Cite this article

Abstract

Expression of the oncoprotein Qin induces tumors in chickens and oncogenic transformation of chicken embryo fibroblasts in culture. We performed a detailed deletion analysis of the C-terminal region of Qin (amino acids 246–451, extending from the winged helix domain to the C-terminus) and identified amino acids 246–379 as important for transformation. The same region mediates homo-oligomerization of Qin as documented in vitro by GST pulldowns and in vivo by coimmunoprecipitation. A 60 amino-acid region within the oligomerization domain is necessary and sufficient for transcriptional repression induced by Qin.

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Acknowledgements

We thank Martin Sonderegger for technical assistance. This work was supported by grants from the National Cancer Institute. CKS was supported by Schroedinger fellowships J1691-GEN and J1874-GEN from the Austrian Science Foundation FWF Austria. This is manuscript number 14968-MEM at The Scripps Research Institute.

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  1. Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, 92037, CA, USA

    Corinna K Sonderegger, Mako Narisawa-Saito & Peter K Vogt

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  1. Corinna K Sonderegger
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Correspondence to Peter K Vogt.

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Sonderegger, C., Narisawa-Saito, M. & Vogt, P. The C-terminal region of cellular Qin oligomerizes: correlation with oncogenic transformation and transcriptional repression. Oncogene 22, 1908–1915 (2003). https://doi.org/10.1038/sj.onc.1206307

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