Abstract
We show that SV40 infection of human mesothelial cells directly causes overexpression of Notch-1, a key cell regulatory gene. Notch-1 induction is achieved at the transcriptional level and requires both the SV40 large T-antigen and the small t-antigen. Notch-1 upregulation is maintained in SV40-transformed human mesothelial clones and in SV40-positive mesotheliomas and derived cell lines. Activation of Notch-1 promotes cell cycle progression and it is required for the growth of SV40-transformed mesothelial cells. Our finding is relevant to the process of SV40-mediated human cell transformation, an effect that cannot be accounted for solely by SV40-Tag inhibition of Rb and p53.
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Accession codes
Abbreviations
- SV40:
-
Simian virus 40
- tag:
-
SV40 large tumor antigen
- tag:
-
SV40 small tumor antigen
- HM:
-
primary human mesothelial cells
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Acknowledgements
This work was supported by RO-1 CA92657, by the Charlotte Geyer Foundation, and by ACS RPG-99-238-01-CAN through a generous donation of Mr Dean Butckovitz, and by the Riviera Country Club, Orland Park, IL, to MC, and by RO-1 CA84065 to Lucio Miele. Lucio Miele provided expertise, reagents and advice for Notch-1 experiments. Harvey I Pass provided the mesothelioma biopsies.
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Bocchetta, M., Miele, L., Pass, H. et al. Notch-1 induction, a novel activity of SV40 required for growth of SV40-transformed human mesothelial cells. Oncogene 22, 81–89 (2003). https://doi.org/10.1038/sj.onc.1206097
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DOI: https://doi.org/10.1038/sj.onc.1206097
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