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  • Oncogenomics
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MLL5, a homolog of Drosophila trithorax located within a segment of chromosome band 7q22 implicated in myeloid leukemia

Oncogene volume 21, pages 4849–4854 (2002)Cite this article

Abstract

Proteins encoded by Polycomb and Trithorax-group (Pc-G and Trx-G) genes regulate developmental fates by maintaining or repressing HOX gene expression, respectively. In a search for candidate myeloid leukemia tumor suppressor genes from a ∼2.5 Mb commonly-deleted segment within chromosome band 7q22, we identified a novel human Trithorax (Trx) family member named MLL5. Trx-G genes encode proteins that modulate transcriptional programs through protein–protein interactions that are mediated by PHD and SET domains, and by binding to DNA via A-T hooks and methyltransferase homology motifs. MLL5 is a homolog of the Drosophila gene CG9007; it encodes a 6.5 kb mRNA that is expressed widely. MLL5 includes a SET domain and a single PHD finger, but lacks A-T hooks and methyltransferase homology domains that are found in MLL. The leukemia cell line RCV-ACV-A carries a heterozygous missense mutation within the PHD domain; however, no mutations within the MLL5 coding region were detected in primary leukemias. MLL5 is a novel mammalian Trx-G gene that might modulate transcription by protein association.

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Acknowledgements

This work was supported by PHS grant CA40046 (to MM Le Beau and KM Shannon), by the Frank A Campini Foundation (to KM Shannon), and by a fellowship grant from the Dr Mildred Scheel Stiftung für Krebsforschung (to CP Kratz). These studies were also facilitated by a collaboration with the Children's Oncology Group (Study Number B24), and by the gift of an ABI 7700 Sequence Detection System from Applied Biosystems.

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Authors and Affiliations

  1. Department of Pediatrics and Comprehensive Cancer Center, University of California, San Francisco, 94143, California, CA, USA

    Brooke M Emerling, Jeannette Bonifas, Christian P Kratz, Shane Donovan, Brigit R Taylor & Kevin M Shannon

  2. Genome Technology Branch, National Human Genome Research Institute, Bethesda, 20892, Maryland, MD, USA

    Eric D Green

  3. Department of Medicine, Section of Hematology/Oncology, and The Cancer Research Center, University of Chicago, Chicago, 60637, Illinois, IL, USA

    Michelle M Le Beau

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  1. Brooke M Emerling
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Correspondence to Kevin M Shannon.

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Emerling, B., Bonifas, J., Kratz, C. et al. MLL5, a homolog of Drosophila trithorax located within a segment of chromosome band 7q22 implicated in myeloid leukemia. Oncogene 21, 4849–4854 (2002). https://doi.org/10.1038/sj.onc.1205615

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