Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Report
  • Published:

Analysis of native WT1 protein from frozen human kidney and Wilms' tumors

Oncogene volume 18, pages 2533–2536 (1999)Cite this article

Abstract

The Wilms' tumor susceptibility gene, WT1, is altered in a subset of Wilms' tumors and encodes a transcription factor with four zinc fingers. Here we describe the isolation of native WT1 protein from frozen normal human kidney and Wilms' tumor samples. Through size exclusion chromatography and Western blot analysis we determined the elution pattern of WT1. The majority of WT1 from adult kidney and Wilms' tumor specimens was found to elute at a size of approximately 120 kDa, consistent with a WT1 homodimer and some WT1 protein was also found in a higher molecular weight complex. In 14 week fetal kidney the majority of the WT1 protein eluted at a size of 80 kDa, suggesting that at this developmental stage the WT1 protein is not present as a homodimer. The identity of complexing partners can now be studied using this approach.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  • Bickmore WA, Oghene K, Little MH, Seawright A, Van Heyningen V and Hastie ND. . 1992 Science 257: 235–237.

  • Brenner B, Wildhardt G, Schneider S and Royer-Pokora B. . 1992 Oncogene 7: 1431–1433.

  • Bruening W and Pelletier J. . 1996 J. Biol. Chem. 271: 8646–8654.

  • Call KM, Glaser T, Ito CY, Buckler AJ, Pelletier J, Haber DA, Rose EA, Kral A, Yeger H and Lewis WH. . 1990 Cell 60: 509–520.

  • Dignam JD, Lebovitz RM and Roeder RG. . 1983 Nucleic Acids Res. 11: 1475–1489.

  • Gessler M, Poustka A, Cavenee W, Neve RL, Orkin SH and Bruns GAP. . 1990 Nature 343: 774–778.

  • Grubb GR, Yun K, Williams BRG, Eccles MR and Reeve AE. . 1994 Lab. Invest. 71: 472–479.

  • Haber DA, Sohn RL, Buckler AJ, Pelletier J, Call KM and Housman DE. . 1991 Proc. Natl. Acad. Sci. USA 88: 9618–9622.

  • Johnstone RW, See RH, Sells SF, Wang J, Muthukkumar S, Englert C, Haber DA, Licht JD, Sugrue SP, Roberts T, Rangnekar VM and Shi Y. . 1996 Mol. Cell. Biol. 16: 6945–6956.

  • Kreidberg JA, Sariola H, Loring JM, Maeda M, Pelletier J, Housman D and Jaenisch R. . 1993 Cell 74: 679–691.

  • Maheswaran S, Park S, Bernard A, Morris JF, Rauscher III FJ, Hill DE and Haber DA. . 1993 Proc. Natl. Acad. Sci. USA 90: 5100–5104.

  • Maheswaran S, Englert C, Bennett P, Henrich G and Haber DA. . 1995 Genes Dev. 9: 2143–2156.

  • Moffet P, Bruening W, Nakagama H, Bardeesy N, Housman DE and Pelletier J. . 1995 Proc. Natl. Acad. Sci. USA 92: 11105–11109.

  • Mundlos S, Pelletier J, Darveau A, Bachmann M, Winterpacht A and Zabel B. . 1993 Development 119: 1329–1341.

  • Pelletier J, Schalling M, Buckler AJ, Rogers A, Haber DA and Housman D. . 1991 Genes & Development 5: 1345–1356.

  • Pritchard-Jones K, Fleming S, Davidson D, Bickmore W, Porteus D, Gosden C, Bard J, Buckler A, Pelletier J, Housman D, van Heyningen V and Hastie N. . 1990 Nature 346: 194–197.

  • Rauscher FJ, Morris JF, Tourney DE, Cook DM and Curran T. . 1990 Science 150: 1259–1262.

  • Reddy JC, Morris JC, Wang J, English MA, Haber DA, Shi Y and Licht J. . 1995 J. Biol. Chem. 270: 10878–10884.

  • Reddy JC and Licht JD. . 1996 Biochimica et Biophysica Acta 1287: 1–28.

  • Renshaw J, Kind-Underwood L and Pritchard-Jones K. . 1997 Genes, Chromosomes & Cancer 19: 256–266.

  • Rupprecht HD, Drummond IA, Madden SL, Rauscher III FJ and Sukhatme VP. . 1994 J. Biol. Chem. 269: 6198–6206.

  • Sakamoto Y, Yoshida M, Semba K and Hunter T. . 1997 Oncogene 15: 2001–2012.

  • Sauer F and Jäckle H. . 1993 Nature 364: 454–457.

  • Schumacher V, Schneider S, Figge A, Wildhardt G, Harms D, Schmidt D and Weirich A. . 1997 Proc. Natl. Acad. Sci. USA 94: 3972–3977.

  • Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T and Deuel TF. . 1996 J. Biol. Chem. 271: 24811–24816.

  • Ye Y, Raychaudhuri B, Gurney A, Campbell CE and Williams BRG. . 1996 EMBO J. 15: 5606–5615.

Download references

Acknowledgements

We thank the Department of Biochemistry I, Prof Wieland, University of Heidelberg, for the access to a FPLC unit and Carsten Bruening for technical help, Dr Martin Teichmann for helpful discussions and Dr H-D Royer and Dr V Schumacher for critically reviewing of the manuscript. The fetal kidney specimen was a kind gift of Prof Zabel, Mainz. This work was supported by a grant from the government of Baden-Württemberg.

Author information

Author notes

  1. Sebastian Iben

    Present address: German Cancer Research Center, Heidelberg, Germany

Authors and Affiliations

  1. Institute of Human Genetics and Anthropology, University of Heidelberg, Heidelberg, D69120, Germany

    Sebastian Iben

  2. Institute of Human Genetics and Anthropology, University of Duesseldorf, Postfach 101007, D40001, Düsseldorf, Germany

    Brigitte Royer-Pokora

Authors
  1. Sebastian Iben
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brigitte Royer-Pokora
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iben, S., Royer-Pokora, B. Analysis of native WT1 protein from frozen human kidney and Wilms' tumors. Oncogene 18, 2533–2536 (1999). https://doi.org/10.1038/sj.onc.1202618

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1202618

Keywords

Search

Advanced search

Quick links