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A mutant form of the rho protein can restore stress fibers and adhesion plaques in v-src transformed fibroblasts

Oncogene volume 18, pages 2117–2128 (1999)Cite this article

Abstract

The organization of polymerized actin in the mammalian cell is regulated by several members of the rho family. Three rho proteins, cdc42, rac and rho act in a cascade to organize the intracellular actin cytoskeleton. Rho proteins are involved in the formation of actin stress fibers and adhesion plaques in fibroblasts. During transformation of mammalian cells by oncogenes the cytoskeleton is rearranged and stress fibers and adhesion plaques are disintegrated. In this paper we investigate the function of the rho protein in RR1022 rat fibroblasts transformed by the Rous sarcoma virus. Two activated mutants of the rho protein, rho G14V and rho Q63L, and a dominant negative mutant, rho N117I, were stably transfected into RR1022 cells. The resulting cell lines were analysed for the organization of polymerized actin and adhesion plaques. Cells expressing rho Q63L, but not rho wt, rho G14V or rho N117I, showed an altered morphology. These cells displayed a flat, fibroblast like shape when compared with the RR1022 ancestor cells. Immunofluorescence analyses revealed that actin stress fibers and adhesion plaques were rearranged in these cells. We conclude from these data that an active rho protein can restore elements of the actin cytoskeleton in transformed cells by overriding the tyrosine kinase phosphorylation induced by the pp60v-src.

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Acknowledgements

We thank Prof Dr Henning for the RR1022 cells and Dr Gisela Banauch for critically reading the manuscript. In addition we thank E Ossendorf for performing the pp60 kinase assay. The work was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 310, A9) to TM and AB and Fonds der Chemischen Industrie to AB.

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  1. Department for Experimental Tumorbiology, Muenster University, Badestr. 9, Muenster, 48149, Germany

    Thomas Mayer, Markus Meyer, Annette Janning, Anke C Schiedel & Angelika Barnekow

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Mayer, T., Meyer, M., Janning, A. et al. A mutant form of the rho protein can restore stress fibers and adhesion plaques in v-src transformed fibroblasts. Oncogene 18, 2117–2128 (1999). https://doi.org/10.1038/sj.onc.1202537

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