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Suppression of src-induced transformed phenotype by expression of tropomyosin-1

Oncogene volume 18, pages 2027–2031 (1999)Cite this article

Abstract

Suppression of high Mr tropomyosins (TMs) is a common feature of transformed cells. Previous work from this laboratory has demonstrated that the isoform 1 of TM, TM1, acts as an anti-oncogene in ras-transformed murine fibroblasts. In this study, we have investigated whether TM1 is a ras-specific suppressor, or a general suppressor protein of the cellular transformation. V-src transformed fibroblasts, which express decreased TM1, were transduced with a full-length cDNA to overexpress TM1. Both the control and the transduced cells expressed v-src kinase at comparable levels. TM1 expressing (src-T1) cells grew at a lower rate in monolayer, exhibited well spread, flat morphology than the control cells. Enhanced expression of TM1 resulted in improved microfilamental architecture. More significantly, src-T1 cells completely failed to grow under anchorage independent conditions. These data demonstrate that TM1 is as an anti-oncogene of functionally diverse oncogenes, and it is a class II tumor suppressor protein.

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Acknowledgements

This work is supported in part by grants from `Race for the Cure', Washington DC chapter of the Susan G Komen Foundation, the American Cancer Society (IRG#204), and the WW Smith Charitable Trust to GLP.

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Authors and Affiliations

  1. Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, 19140, Pennsylvania

    G L Prasad

  2. USA

    G L Prasad

  3. Department of Experimental Medicine and Pathology, University LaSapienza, Rome, 00161, Viale Regina Elena 324, Italy

    L Masuelli

  4. Department of Ob-Gyn, and Biochemistry, LSU Medical Center, New Orleans, 70112, Louisiana

    M H G Raj

  5. Division of Hematologic products, CBER, FDA, Bethesda, 20892, Maryland, USA

    N Harindranath

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Prasad, G., Masuelli, L., Raj, M. et al. Suppression of src-induced transformed phenotype by expression of tropomyosin-1. Oncogene 18, 2027–2031 (1999). https://doi.org/10.1038/sj.onc.1202264

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