Abstract
Throughout infection, plant-parasitic nematodes activate a complex host defense response that will regulate their development and aggressiveness. Oxylipins—lipophilic signaling molecules—are part of this complex, performing a fundamental role in regulating plant development and immunity. At the same time, the sedentary root-knot nematode Meloidogyne spp. secretes numerous effectors that play key roles during invasion and migration, supporting construction and maintenance of nematodes' feeding sites. Herein, comprehensive oxylipin profiling of tomato roots, performed using LC–MS/MS, indicated strong and early responses of many oxylipins following root-knot nematode infection. To identify genes that might respond to the lipidomic defense pathway mediated through oxylipins, RNA-Seq was performed by exposing Meloidogyne javanica second-stage juveniles to tomato protoplasts and the oxylipin 9-HOT, one of the early-induced oxylipins in tomato roots upon nematode infection. A total of 7512 differentially expressed genes were identified. To target putative effectors, we sought differentially expressed genes carrying a predicted secretion signal peptide. Among these, several were homologous with known effectors in other nematode species; other unknown, potentially secreted proteins may have a role as root-knot nematode effectors that are induced by plant lipid signals. These include effectors associated with distortion of the plant immune response or manipulating signal transduction mediated by lipid signals. Other effectors are implicated in cell wall degradation or ROS detoxification at the plant–nematode interface. Being an integral part of the plant's defense response, oxylipins might be placed as important signaling molecules underlying nematode parasitism.
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Introduction
Meloidogyne species of root-knot nematodes (RKN) are one of the main devastating plant parasites, infecting an estimated 5000 plant species1,2,3. The RKN are obligatory sedentary endoparasitic biotrophs, with more than 90 known Meloidogyne species distributed ubiquitously. The most destructive species are M. incognita, M. arenaria, M. hapla and M. javanica, causing crop losses amounting to hundreds of billions of US dollars each year4,5,6. Successful parasite penetration, migration, establishment and maintenance rely mainly on the secretion of effectors through the stylet that promote and establish an intimate long-term interaction with the host7,8,9. These effectors are predominantly synthesized by the esophageal glands, two subventral and one dorsal; and other organs, such as amphids and cuticle, also participate in the parasitic secretion10. Multiple effectors of plant-parasitic nematode species have been successfully isolated and characterized using transcriptome, secretome and RNAi approaches11,12,13,14,15,16,17,18,19. Of the various effector categories, some effectors have been found to act as immunomodulators that manipulate, via mimicry or suppression, the host immune system.
Although lipids are vital cellular components, increasing evidence suggests their supplementary role in plant immunity. Lipids and their derivative signaling molecules are involved in responses to biotic and abiotic stresses20,21. Several studies have suggested that these compounds are subject to pathogen manipulation and interference. Nematode-derived fatty acid- and retinol-binding (FAR) proteins are predominantly secreted into the host cell for developmental processes, but may facilitate parasitism by interfering with lipid signaling related to plant defense22,23,24. Similarly, in a recent study, overexpression of the M. javanica FAR effector (mj-far-1) rendered plants more susceptible to nematodes infection25,26. One of the universal plant-defense mechanisms upon pathogen infection is the production of a wide variety of compounds collectively termed oxylipins. Oxylipins are a large family of oxidized polyunsaturated fatty acids distributed throughout the plant and animal kingdoms. These highly dynamic metabolites serve as signaling molecules and regulate many different biological functions, such as stress and developmental processes, and contribute to the plant's innate immune response directly as antimicrobial and/or antifungal factors, or indirectly as secondary messengers, to modulate the plant–pathogen interaction27,28,29. The biosynthesis of plant oxylipins is initiated by the release of linolenic or linoleic acids from cell membranes, which are converted to fatty acid hydroperoxides either through an enzymatic pathway by the action of 9-/13-lipoxygenases (9-/13-LOX), α dioxygenase (α-DOX), or a non-enzymatic pathway in the presence of singlet oxygen30,31,32. The hydroperoxide products serve as substrates for at least six alternative enzymatic pathways, resulting in the generation of hundreds of different oxylipin molecules with diverse structures and functions, classified as hydroperoxides, hydroxides, ketotrienes, ketodienes, epoxides and diols, trials, dicarboxylic acids, and ketols, among others. Among the oxylipins, the jasmonate (JA) group has been well-characterized and is shown to mediate plants’ defense response against necrotrophic pathogens and herbivores33,34 .While the functions of the vast majority of oxylipins have yet to be elucidated, their production has been shown to change dramatically upon microbial and fungal pathogen invasion, suggesting an important role in defense35,36,37,38. Deficiencies in the LOX pathway lead to alterations in the plant’s response to pathogen attack, supporting the prominent role of oxylipins in the establishment of resistance39. Oxylipins synthesized via the 9-LOX pathway are involved in plant development, including root architecture, senescence and seed germination, and in the stimulation of plant defense upon pathogen attack. The 9-LOX derivatives are among the most active oxylipins in terms of antimicrobial and/or antifungal activity and, in their ability to regulate the programmed cell death responses 40,41,42,43,44,45.
Recent findings have implicated lipid metabolic pathways in the defense responses of plants to RKN46,47,48. For example, microarray-based expression profiling indicated that several fatty acid metabolism genes, including LOX, patatin-like protein 1 (PLP1), and 12-oxophytodienoate reductase (OPR), are induced in susceptible soybean roots in response to soybean cyst nematode infection49. Similarly, LOX activity increased in pea root50 and soybean roots49 upon infection with cyst nematodes. Moreover, degradation of trophic cells, indicating a hypersensitive response, is accompanied by increased activity of pea LOX51. Genetic evidence of the involvement of the maize 9-LOX-biosynthesis pathway in resistance mechanisms to RKN was further provided by the analysis of the lox3-knockout mutant of maize, which displayed increased attraction and susceptibility to RKN compared to a near isogenic wild-type line52. Similarly, Ozalvo et al.25 showed that in Arabidopsis, the 13-LOX enzyme, LOX4, operates as a root-specific inducer of major defense signaling pathways in response to nematode infection. Recently, a wide screening of oxylipins against M. javanica second-stage juveniles (J2) showed that (9S,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid (9-HOT) application attenuates nematode viability53. 9-HOT is produced by the 9-LOX pathway, controlling root development and inducing cell-wall defense responses such as callose accumulation and reactive oxygen species production40. We hypothesize that the dynamics of host lipid-mediated signaling induces changes in M. javanica infective juveniles' gene-expression profile associated with parasitism. In this study, we report the first comprehensive oxylipin profiling of tomato roots (Solanum lypopersicum cv. Avigail 870) challenged by M. javanica, using LC–MS/MS, to provide novel insight into the plant response involving fatty acid-derived molecules upon RKN attack. By using RNA-Seq transcriptome analysis of M. javanica exposed to the oxylipin 9-HOT and tomato protoplasts, we identified novel potential M. javanica transcripts and effectors which might be classified as early mediators regulating nematode parasitism.
Results and discussion
Oxylipin biosynthesis is rapidly induced upon tomato root infection by M. javanica
To evaluate alterations in oxylipin-mediated defense pathways during tomato root infection by the RKN M. javanica, we performed a quantitative analysis of tomato root oxylipins at 5, 15 and 28 days after nematode inoculation (DAI) corresponding to the time of feeding-site formation by infective juveniles, nematode development into J3–J4 stages, and female maturation, respectively as well as of noninoculated roots at the same time points to assess changes in oxylipins accompanying nematode parasitism. A comprehensive oxylipin metabolomic analysis was conducted using the LC–MS/MS platform for quantitative evaluation, and approximately 100 oxylipins were identified (Fig. 1). As shown, inoculated root tissues exhibited an evident alteration in most of the measured oxylipins upon inoculation with RKN J2 compared to noninoculated roots, the latter generally exhibiting no significant changes (Table 1). For oxylipins originated from the LOX pathway—the ketones 13-keto-9(Z),11(E)-octadecadienoic acid (13-KOD) and 9-keto-10(E),12(Z),15(Z)-octadecatrienoic acid (9-KOT), as well as from the reductase pathway—the hydroxides 9-HOT and 13-HOT, we observed a sharp accumulation 5 DAI, followed by a lesser accumulation 28 DAI. Oxylipins of the epoxy alcohol synthase pathway, generating the triol group, followed a similar trend, where 9,10,13-trihydroxy-11(E)-octadecenoic acid (9,10,13-THOD), 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoate (9,12,13-THOD), 9,10,13-trihydroxyoctadecenoic acid (9,10,13-THOM) and 9,12,13-trihydroxyoctadecenoic acid (9,12,13-THOM) were upregulated at the early time point and gradually decreased as infection proceeded. The presence of oxylipins of the HPL(Hydroperoxide lyase) pathway—azelaic acid and traumatic acid—was measured as well; a significant increase was only observed for azelaic acid 5 DAI compared to noninoculated roots, followed by a clear reduction 15 and 28 DAI. A similar pattern of induction 5 DAI was detected with the α-DOX product 2(R)-hydroxy-9(Z),12(Z),15(Z)-octadecatrienoic acid (2-HOT). Measurement of oxylipins of the 9-allene oxide synthase (9-AOS) pathway—10-oxo-11-phytoenoic acid (10-OPEA) produced from C18:2, and 9-hydroxy-10-oxo-octadecaenoic acid (9OH-10KOM) and 9OH-12KOM belonging to the ketol-group products of auto-oxidation of allene oxides, demonstrated increased accumulation 5 DAI which was sustained 15 DAI (Fig. 1). Among the JA, increased accumulation of JA and JA-Ile was observed 5 DAI compared to noninoculated root tissues, along with a gradual decrease as the infection proceeded. Evidence for the functional role of JA in regulating plant defense response to nematode infection is controversial52,54,55,56,57,58. To settle this conflict, a recent study by Gleason et al.59 has brought new insight to this issue, positioning the JA precursor 12-OPDA, but not JA/JA-Ile, as a key defense signaling molecule involved in regulating plant susceptibility to nematodes59. Our results indicate that while JA and JA-Ile were induced 5 and 15 DAI during compatible interactions with the nematodes, 12-OPDA levels were not affected by nematode infection at the tested time point. In Gleason et al.59 study, OPDA accumulation was already detected within 2 DAI. In our study, the earliest time point of 5 DAI might therefore have been too late for detecting differences in 12-OPDA levels. Taken together, our results put several oxylipins, among them the hydroxides, triols, ketones, epoxides, ketols and the JA group at the interface during the early time point of the parasitic interaction.
M. javanica infection induces expression of tomato oxylipin-biosynthesis genes LOX1.2, AOS1, OPR2 and α-DOX1
To correlate oxylipin occurrence with genetic biosynthetic pathways, we used the GUS–promoter bioassay for spatial and temporal expression of oxylipin-biosynthesis genes LOX1.2, AOS1, OPR2 and αDOX1. Transgenic tomato hairy root lines (line 870) carrying pLOX1.2–GUS, pAOS1–GUS, pOPR2–GUS and pα-DOX1-GUS constructs, and a negative control consisting of hairy root lines carrying empty pCAMBIA2300 vector, were tested for GUS activity at 2, 5, 15 and 28 DAI compared to noninoculated transgenic tomato hairy root lines and a negative control (Fig. 2). LOX's involvement in the plant response to RKN has been previously reported46,47,48. LOX1.2 is a 9-LOX gene (solyc01g099210.2.1) that is homologous to LOX1 in Arabidopsis, located in the cytosol and known to catalyze the hydroperoxidation of linoleic acid, and thus be involved in the pathway of 9-oxylipin biosynthesis (UniProtKB—P38416, LOXB_SOLLC). LOX1.2 expression was observed as a mild signal in the vascular system of the forming gall 5 DAI (Fig. 2C1), with only a faint signal observed 15 DAI and 28 DAI (Fig. 2D1,E1). These results are in good agreement with the oxylipin analysis where we observed that the 9-LOX products, including azelaic acid, a mobile product of HPL that primes systemic acquired resistance60, were elevated 5 DAI and downregulated 28 DAI. The ketones synthesized by LOX, 9-KOT and 13-KOD, as well as through hydroxide synthesis by reductase, 9-HOT, were induced 5 DAI with a clear reduction 28 DAI. Interestingly, these oxylipins have been shown to exert significant nematicidal properties against J253, as well as antimicrobial activity42. Among the products of the epoxy alcohol synthase pathway, the trihydroxy oxylipins 9,10,13-THOD, which have been reported to reduce infection by the fungus Blumeria graminis in barley, were upregulated 5 DAI, and decreased 15 and 28 DAI. Similarly, 9,12,13-THOD and 9,12,13-THOM, which are known to have antimicrobial activity, were upregulated 5 DAI and downregulated 15 and 28 DAI38,53,61.
Next, the expression of 13-AOS1 (solyc04g079730.1.1), located in the thylakoid membrane and involved in JA biosynthesis (UniProtKB—K4BV52, AOS1_SOLLC), was studied. AOS1 reporter lines exhibited GUS staining in the vascular cylinder attached to the developing gall 15 DAI (Fig. 2D2), which strongly increased by 28 DAI in the developed gall (Fig. 2E2).
Functional analysis of AOS1 in rice indicates that rice plants overexpressing AOS1 are less susceptible to M. graminicola62. Similarly, Arabidopsis thaliana AOS mutant dde2 shows more galling by M. hapla than the wild type59. However, being a wound-inducible gene63,64, AOS1 expression at this late time point might indicate that a localized wound response is induced by the developing nematode65. OPR2 (solyc01g0103390.2.1) is known to be weakly expressed in roots and to be involved in the oxylipin-biosynthesis pathway, but as it belongs to the type 1 subfamily, this isoform is not predicted to be a major contributor to JA biosynthesis66,67 (UniProtKB—Q9FEX0, OPRL_SOLLC). The RKN-inoculated OPR2 promoter–GUS reporter line only showed a strong GUS signal 28 DAI in mature galls Fig. 2E3). Strassner et al.66 demonstrated that in contrast to LeOPR3, wounding does not induce LeOPR1 or LeOPR2 expression and that LeOPR2 is expressed at comparatively low levels in tomato roots, leaves, and flowers. However, GUS detection within the mature gall implicated LeOPR2 in a late response, although the physiological function in the mature gall remains obscure. α-DOX1 (solyc02g087070) is a fatty acid-hydroperoxidase located in the endomembrane system, involved in many processes, such as response to oxidative stress, lipid metabolic processes and a salicylic acid (SA) stimulus (UniProtKB—Q69F00 (Q69F00_SOLLC))36,68,69,70. Similarly, α-DOX1 exhibited GUS staining associated with the adult nematode within the developed gall 28 DAI (Fig. 2E4). No staining was observed in the negative controls (Fig. 2A5–E5). α-DOX1 has been previously detected in tomato and Arabidopsis roots tissues and its generated oxylipins have been suggested to mediate the response of roots to several environmental stresses68,70. Our analysis indicated that α-DOX1 is expressed within the mature gall. 2-HOT, the main product of α-DOX, which has been shown to be upregulated by bacterial inoculation and herbivore infestation61,71, was found to be significantly upregulated by RKN inoculation 5 DAI. The lack of synchronized expression of the biosynthesis genes and their accumulated products at early time points, observed for OPR2 and α-DOX1, might be explained by a low level of expression requirement or much earlier expression occurring upon infection. Overall, multiple expression patterns of oxylipins derived from the diverse branched pathways might be the result of plant immunity and/or induced by the nematode for successful establishment. As noted by Prior et al.72 and Iberkleid et al.73, nematodes' FAR protein binds to linolenic and linoleic acids, which are precursors of the oxylipin molecules, and have further been found to inhibit LOX-mediated products. Our results indicate that upregulation of oxylipin biosynthesis is a primary response to nematode inoculation, and as such must be counteracted by an adequate nematode defense response.
RNA-seq analysis of M. javanica J2 following exposure to 9-HOT and tomato protoplasts
To identify transcripts and effectors that are subject to plant host lipid-signaling regulation, the oxylipin 9-HOT, which—among other oxylipins—is induced in tomato roots by RKN infection (Fig. 1), was chosen as the inducer. Similarly, in an attempt to mimic exposure to endogenous metabolites secreted within plant tissue during the parasitic stage, J2 were exposed to tomato protoplasts. Freshly hatched J2 incubated in MES buffer or MES + ethanol served as controls for tomato protoplasts and the studied oxylipin, respectively. Altogether, eight cDNA libraries were constructed, generating a total of 170,786,458 reads, 121,161,460 reads after quality and adaptor trimming by Trimmomatic version 0.3574. The eight libraries represented: (1) biological duplicates of freshly hatched J2 with 15,208,788 reads; (2) biological duplicates of J2 exposed to protoplasts for 3 h with 15,572,666 reads; (3) biological duplicates of J2 exposed to 9-HOT for 3 h with 14,677,503 reads; (4) biological duplicates of J2 in MES + ethanol with 15,121,772 reads. All RNA-Seq raw data reads were uploaded to NCBI under BioProject Accession PRJNA480605. From the 112,732,357 high-quality paired-end reads, 72.01% of the freshly hatched J2, 67.11% of the J2 exposed to protoplasts, 52.78% of the J2 exposed to 9-HOT and 73.01% of the J2 exposed to MES + ethanol were mapped to the reference genome of M. javanica, available on WormBase ParaSite BioProject PRJEB8714 and sequenced by Blanc-Mathieu et al.75 (Table 2).
Uncovering transcriptomic changes in M. javanica J2 upon exposure to oxylipin 9-HOT and other plant signals
To measure transcript regulation of M. javanica J2 by 9-HOT and plant signals, we measured changes in gene expression of infective J2 exposed to tomato protoplasts compared to their control (MES), and of infective J2 exposed to 9-HOT compared to their control (MES + ethanol). Statistical analysis of the differentially expressed genes (DEG) was performed using the DESeq2 package76. The threshold for DEG was FDR ≤ 0.001 and log2 fold change (FC) smaller than − 2 or greater than 2. Overall 7530 DEG were identified among the treatments—J2 exposed to protoplasts and J2 exposed to 9-HOT—compared to their respective controls. Principal component analysis (PCA) was conducted to determine and visualize the significant correlation between the different treatments using R (version 3.0.0) (http://www.R-project.org) and the FactoMineR R package77. PCA of the transcriptomic data was performed for all expressed gene profiles (Fig. 3A). The two dimensions made up 98.51% of the total variance, indicating that most of the factors included in the data were responsible for the significant variation in DEG between treatments. Dimension 1 accounted for 97.46%, and dimension 2 for 1.05% of the variation. The first and second PC axis separated the freshly hatched J2 exposed to 9-HOT from all other groups. Taken together, these results suggest that the different treatments can be divided into two major expression profiles of DEG: (1) freshly hatched J2, J2 exposed to protoplasts and MES + ethanol and (2) J2 exposed to 9-HOT, the latter demonstrating the most variation (Fig. 3A).
Next, all 7512 DEG were subjected to Venn diagram analysis illustrating DEG distribution among treatments (Fig. 3B). A total of 6085 DEG (81%) and 1057 DEG (14.1%) were found to be expressed exclusively in the 9-HOT and protoplast treatments, respectively, and 370 DEG (4.9%) were expressed in both treatments (common DEG) (Fig. 3B.1). A total of 4580 DEG were found to be upregulated (83.3%), with 3813 (83.3%) and 751 (16.4%) expressed in the 9-HOT and protoplast treatment, respectively, and 16 (0.3%) expressed in both treatments (Fig. 3B.2). GO enrichment analysis of the annotated DEG revealed several enriched biological processes (Fig. 3C.1) and molecular functions (Fig. 3C.2). Detailed analysis of identified DEG following exposure to protoplast and 9-HOT revealed that the key enriched biological processes were negative regulation of endopeptidase (GO:0061135), peptidyl-proline modification (GO:0031543), glycosylceramide catabolic process (GO:0004348), cellulose metabolic process (GO:0030243), galactosylceramide metabolic process (GO:0006683). The key enriched molecular functions included polygalacturonase activity (GO:0004650), calcium ion binding (GO:0005509), pectate lyase activity (GO:0030570), endopeptidase activity (GO:0004175), cellulose activity (GO:0030243; 0030245), and endopeptidase inhibitor activity (GO:0004866) (Fig. 3C).
To further classify the observed changes, all 7512 DEG were analyzed for Pathway enrichment using the webserver of KOBAS 3.078 KEGG, GO and PANTHER database enrichment analysis. When compared to Loa loa for glutathione metabolism (loa00480), which plays important roles in antioxidant defense79, 15 DEG were upregulated and 7 DEG downregulated, for a total of 22 out of 25 known genes involved in this pathway (Fig. S1A). In the Wnt signaling pathway (loa04310), known to regulate crucial aspects of cell-fate determination during embryonic development80, 24 DEG were upregulated and 5 DEG were downregulated—29 out of the 65 known genes represented in this pathway (Fig. S1B). In the fatty acid biosynthesis pathway (loa00061), which is a precursor for a variety of important building blocks21,81,82, 8 DEG were upregulated , 10 out of the 10 known genes. For retinol metabolism (loa00830), a total of 6 DEG were upregulated out of 6 known genes in this pathway. All of these DEG were enriched following exposure to 9-HOT. Following exposure of J2 to protoplast treatment, the calcium signaling pathway (loa04020) was represented by 27 upregulated DEG out of a total of 57 genes known to be involved in this pathway. In the inositol phosphate metabolism pathway (loa00562), 9 DEG were upregulated out of a total of 38 known genes in this pathway. In the phosphatidylinositol signaling system (loa04070), 9 DEG were upregulated out of 57 known genes in this pathway.
9-HOT application regulates the expression of genes encoding carbohydrate-active enzymes (CAZymes) related to cell wall modification and degradation
To further evaluate the effect of 9-HOT and protoplasts on CAZymes, we investigated families of structurally related catalytic and carbohydrate-binding modules (CBM) of enzymes that degrade, modify or create glyosidic bonds. We focused on the DEG encoding CAZymes related to cell wall biosynthesis, modification and remodeling82 (Fig. S2). Differential expression of four categories of CAZYmes were represented following exposure to 9-HOT and protoplasts, i.e., genes encoding carbohydrate esterase (CE), glycoside hydrolase (GH), glycosyl transferase (GT) and polysaccharide lyase (PL) (Figure S2A). Among the CAZyme categories involved in cellulose degradation (Fig. S2B), two families of GH were differentially expressed following protoplast and 9-HOT treatments. These included GH5 and GH7 presented by endo-1,4-β-glucanase/cellulase and β-glucosylceramidase and chitosanase. Among the hemicellulose-degrading genes (Fig. S2C), two categories were represented by GH31 and CE1, such as α-galactosidase, α-mannosidase, glucosyltransferase acetyl transferase and carboxylesterase.
9-HOT induces major differences in nematode's effector-encoding gene expression
Given that we were interested in genes involved in governing parasitism, i.e., effectors, our next step was an in-silico analysis to identify differentially expressed transcripts that might encode secreted effectors. DEG that contained a predicted signal peptide according to SignalP5.083 and which do not carry TMHMM (transmembrane alpha helix motifs) were subjected to Venn diagram analysis illustrating DEG distribution among treatments (Fig. 4A). A total of 913 DEG with a signal peptide were identified (12.2% of total DEG). Among these, 707 DEG (77.4%) and 116 DEG (9.9%) were expressed in the 9-HOT and protoplast treatments, respectively, and 90 DEG (12.7%) were expressed in both treatments (Fig. 4A.1). A total of 367 DEG with a signal peptide were found to be upregulated (33.7%) (Fig. 4A.2), and 597 (54.9%) DEGs were downregulated (Fig. 4A.3).
All DEG that were upregulated by 9-HOT treatment were analyzed for GO terms (Fig. 4B) in each of the three main categories (biological process, molecular function and cellular component classification) of the GO classification. The GO cellular component classification of the DEG indicated 12% of extracellular region and 88% of the membrane part. Biological process was represented by 10 categories: 31% by negative regulation of peptidase activity (GO:0010466), 30% by regulation of endopeptidase activity (GO:0052548), about 4–7% each by chitin, ceramide, glucosamine, glycosaminoglycan and glycolsphingolipid catabolic process (GO:0006032, GO:0046514, GO:1901072, GO:0006027 and GO:0046479, respectively) (Fig. 4B). Molecular function was represented by 11 categories: 31% by serine-type endopeptidase inhibitor activity (GO:0004867), 18% by metalloendopeptidase activity (GO:0004222), 9% by carboxypeptidase activity (GO:0004180). Next, we validated the expression profile of predicted effectors in protoplast- and 9-HOT-treated M. javanica J2. For that purpose, seven selected DEG from the M. javanica J2 transcriptome were further confirmed and validated by quantitative reverse transcription (qRT)-PCR. Four downregulated genes and three upregulated genes were selected for quantitative analyses, on the basis of being potentially secreted and involved in the pathogenic process and carrying a signal peptide. One of the DEG was found in J2 exposed to protoplasts: protoplasts#1 encoding DB domain-containing protein (M.javanica_Scaff1102g012786); six were found in J2 exposed to 9-HOT: 9-HOT#1—unknown gene (M.javanica_Scaff10526g059067), 9-HOT#2—SCP domain-containing protein (M.javanica_Scaff139g002482), 9-HOT#3—unknown gene (M.javanica_Scaff8981g053951), 9-HOT#4—putative esophageal gland cell secretory protein 3 (M.javanica_Scaff2606g024064), 9-HOT#5—calycin-like domain (M.javanica_Scaff24242g089056), 9-HOT#6—triacylglycerol lipase (M.javanica_Scaff6853g045742), all of which are shown in Fig. S3. For all qRT-PCR analyses, two housekeeping genes were chosen as reference genes for M. javanica: endogenous reference genes 18S and EF-1α (Table 1S). For all tested transcripts, our analysis remained similar to the transcriptomic trend of the FC data.
Differentially regulated effector-encoding genes are implicated in cell wall modifications, stress response, plant immune suppression and nematode development, enabling parasitism
Among the 346 upregulated DEG in the 9-HOT treatment were genes implicated in nematode growth and development, such as MLT-10, the cuticlin-1, epicuticulin gene family and collagen, all of which participate in various cellular and developmental processes required for nematode molting and fecundity. Within the root tissues, RKN undergo three molting stages; in each molt, the makeup of the cuticle surface coat's compounds changes, one among many strategies acquired by plant-parasitic nematodes to avoid plant immunity84. Similarly, previous studies have shown that hormones and different compounds secreted by the roots trigger changes in the surface cuticle of sedentary plant-parasitic nematodes10,85.
In addition, a group of genes implicated in oxidation–reduction activity required for coping with oxidative stress response were induced by 9-HOT (i.e., glutaredoxin, thioredoxin-like domain). Similarly, effectors containing a C-type lectin, which was found to delay the oxidative burst in tobacco leaves following infection by M. graminicola86, were upregulated following exposure to 9-HOT (M.javanica_Scaff6180g042809) and protoplasts (M.javanica_Scaff16387g074472) (Fig. 4A). In addition, genes involved in lipid modification (several genes of triacylglycerol lipase, calycin domain) were also differentially expressed following 9-HOT treatment. Differential regulation of several proteases was observed: a carboxypeptidase, serine carboxypeptidase (SCP), was studied in depth and was shown to play a critical role in the development, invasion, and pathogenesis of certain parasitic nematodes and other animal pathogens87. Another protease, a serine proteinase, that was induced has also been shown to be involved in mediation of host invasion by the parasitic nematode Steinernema carpocapsae87. Similarly, alteration in the expression of chorismate mutase (CM) and venom allergen-like protein (Vap2), well-studied effectors that are involved in suppression of defense reactions of the host cell during the infection stages, were detected14,87,88,89,90,91. Papain inhibitor, which might be related to pathogen effectors that inhibit apoplastic papain-like cysteine protease (PLCP) which are strongly associated with the effector triggered immunity (ETI) response92, were strongly upregulated upon 9-HOT treatment. An extensive representation of genes involved in cell wall modification and remodeling, carrying a signal peptide, were altered upon 9-HOT and protoplast exposure, e.g., effectors carrying a Rare lipoprotein A domain found in several effectors, such as Mc-EXP1, GrEXPB1 and GrEXPB2, associated with cell wall extension in M. chitwoodi and G. rostochiensis, respectively93,94, were upregulated upon 9-HOT application. Additional observed differentially regulated genes were involved in cell wall degradation and modification (Fig. S2), including cellulose binding protein (CBP)—a nematode excretion protein that appears to be associated with the breakdown of cellulose present in the plant cell wall95,96—and pectate lyases, known to play a key role in pectin degradation by catalyzing the random cleavage of internal polymer linkages (endopectinases). Similarly, pectate lyases have been isolated from several sedentary plant-parasitic nematodes, such as species of Heterodera, Globodera, and Meloidogyne97,98,99, and have been shown to be released into the plant tissue through the stylet of the nematode. GH family 38 (GH38) α-mannosidase was upregulated by 9-HOT; this protein is involved in α-mannose cleavage, carrying hemicellulose activity, and has been identified in several phytopathogenic nematode species100. In addition, several endoglucanases belonging to the GH5 family were differentially expressed upon 9-HOT treatment (Fig. S2); these have been shown to facilitate penetration and migration into root tissue and were localized to the esophageal glands of infective juveniles95,101,102. All of these genes are part of a cocktail of cell wall-degrading and modifying enzymes that are thought to soften and degrade the structure of plant cell walls during nematode migration and to facilitate infection95,103. Their fluctuation upon 9-HOT and protoplast treatment might indicate tight regulation governed by oxylipin signals, among others.
Triacylglycerol lipase and MLT10-like, predicted effectors, are exclusively localized to the M. javanica esophageal glands
Additional effectors that may facilitate plant–nematode interactions through manipulation of the plant defense system, or are required for nematode developmental processes, and which were localized to the esophageal glands upon 9-HOT application, are triacylglycerol (TAG) lipase, which functions in TAG release from lipid droplets by lipolysis in the peroxisome82. Interestingly, modulation of plant peroxisomes in giant cells by sedentary RKN has been described previously104; and molting cycle MLT-10-like, required for nematode development. Using fluorescence in situ hybridization (FISH), we designed a Cy5-probe to specifically target the spatiotemporal expression of several putative effector-encoding genes derived from the above DEG carrying a signal peptide. FISH results, shown in Fig. 5, localized TAG lipase exclusively to the dorsal and two subventral glands upon J2 exposure to 9-HOT (Fig. 5A1–4), compared to its control (i.e., J2 exposed to MES + ethanol) that showed no fluorescent signal (Fig. 5A5–8). Similarly, MLT-10 was localized to the subventral glands upon J2 exposure to 9-HOT (Fig. 5B1–4), compared to its control (J2 exposed to MES + ethanol) with no fluorescent signal.
These results strengthened our assumption that the DEG containing a signal peptide are potential secreted effectors. Additional analysis of TAG lipase and MLT-10-like by qRT-PCR at different stages of M. javanica development further correlated their expression with parasitism (Fig. S4).
Conclusions
Despite enormous progress in the discovery and identification of nematode effectors in the last decade7,15,105,106,107,108,109,110, less is known about their function and the specific signals required for their induction. Our transcriptomic studies of M. javanica provide evidence of transcripts with homology to previously reported plant-parasitic nematode effectors, as well as unknown secreted proteins, all induced by 9-HOT. Together with the oxylipin profile analysis, it seems that oxylipins, while part of the plant's defense response, might play an important signaling role in regulation of the nematode transcriptome. Among the differentially regulated predicted effectors, several were further confirmed by FISH analysis as effectors located within the esophageal glands. Taken together, these results placed oxylipins as early modulators of plant defense signals, as well as important signals regulating nematode parasitism. The implication of G-protein coupled receptor (GPCR) as an oxylipin receptor, shown previously by Affeldt et al.111 and Lahvic et al.112, should place this group of nematode receptors as important mediators of parasitic behavior. This interaction remains to be studied.
Methods
Nematode culture and inoculum preparation
M. javanica were multiplied on tomato plants (Solanum lypopersicum cv. Avigail 870) in a greenhouse. Nematode egg masses were extracted by cutting roots into pieces and macerating in 0.05% (v/v) sodium hypochlorite (NaOCl) in a blender113. The resulting suspension was passed through a set of three sieves (120, 60 and 30 µm). The debris was discarded, while the eggs deposited on the 30-µm sieve were transferred to a 50-mL test tube. Centrifugal flotation with 40% (w/v) sucrose at 6000 rpm for 10 min was performed; the supernatant, containing the eggs, was poured onto a 30-µm sieve and washed with tap water, and eggs were collected in MES buffer113 and sterilized as described by van Vuuren and Woodward114.
Subsequently, eggs were transferred onto a 30-µm sieve and suspended in 5 mL MES buffer in a petri dish. The petri dish was placed in a growth chamber at 26 °C under dark conditions till hatching (5–6 days)115.
J2 exposure to protoplast treatment
Sterilized tomato seedlings were grown at 26 °C under 16 h daylight in sterile plates on standard-strength Gamborg's B5 Salt medium (DUCHEFA, Haarlem, The Netherlands), supplemented with 2% (w/v) sucrose and solidified with 0.8% (w/v) Gelrite agar (DUCHEFA). Roots were subcultured on the same medium with one root section per petri dish (MINIPLAST, Ein Shemer, Israel) and incubated in a growth chamber at 26 °C in the dark for 2 weeks. Protoplasts were released and isolated from about 40 plates of roots using Demidchik and Tester's116 protocol. Fresh protoplasts were incubated with 60,000 freshly hatched sterilized J2 at 26 °C in the dark for 3 h.
J2 exposure to 9-HOT treatment
The 9-HOT oxylipin used in this study was purchased from CAYMAN CHEMICAL Company, diluted with MES buffer to a final concentration of 10 µM. Freshly hatched sterilized J2 were incubated in vials (500 J2 per vial for a total of 15,000 juveniles for each oxylipin treatment) containing 9-HOT or 0.01 M MES + ethanol (to a final concentration of 10 mM, as a control) for 3 h at 26 °C in the dark.
cDNA library preparation and high-throughput sequencing
Total RNA extraction from freshly hatched J2, and J2 exposed to protoplasts, 9-HOT and MES + ethanol, was performed with Trizol, using total RNA extraction from the Caenorhabditis elegans bioprotocol (2011) with steps 10–12 being replaced by the use of the Arcturus PicoPure RNA Isolation Kit, APPLIED BIOSYSTEMS (Foster City, CA, USA), and stored frozen at − 80 °C till further processing117. Quality measurements for total RNA were performed using TapeStation 2200 (Agilent). The RIN (RNA integrity number) values of all 8 samples were between 7 and 10.
Library preparation and data generation
Eight RNA-Seq libraries were produced using the NEBNEXT ULTRA Directional RNA Library Prep Kit for Illumina (NEB, cat no. E7420) according to the manufacturer's protocol and starting with 100 ng of total RNA. The mRNA pull-up was performed using the Magnetic Isolation Module (NEB, cat no. E7490). The eight libraries were mixed in a single tube at equal molar concentrations. RNA-Seq data were generated using the Illumina NextSeq Machine and Nextera Preparation Kit at the Technion—Institute of Technology in Haifa, Israel. Eight paired-end RNA-Seq libraries of two replicates for each condition were sequenced.
Differential expression analysis
The sequences were trimmed for adaptor and low-quality sequence removal using Trimmomatic software74. Cleaned sequences were mapped using Bowtie2118 and quantified using the RSEM method119 to the reference genome of M. javanica (accession no. GCA_900003945.1). The annotated proteins of the nematode were searched for signal peptides using the software SignalP 5.083.
DEG were identified using the DESeq2 R package76. To create the Venn diagrams, we used Venny website http://bioinfogp.cnb.csic.es/tools/venny/120. All RNA-Seq datasets were uploaded to the SRA NCBI database under BioProject Accession No. PRJNA480605.
CAZyme annotation
The search for and functional annotation of CAZymes (automated carbohydrate-active enzyme annotation) was performed using the CAZY database (http://www.cazy.org/) according to Lombard et al.121. We assigned the M. javanica (accession no. GCA_90003945.1) proteins to the CAZY database using the dbCAN2 meta server (http://bcb.unl.edu/dbCAN2index.php).
Plant material and growth conditions
Tomato (Solanum lycopersicon cv. Avigail 870) seeds were sterilized with 1.4% (v/v) NaOCl for 10 min, washed three times with sterile water for 5 min each, and then planted on standard strength Gamborg's B5 salts medium (DUCHEFA), supplemented with 2% sucrose and solidified with 0.8% Gelrite agar (DUCHEFA). Seeds were kept in a growth chamber at 26 °C under a 16/8-h photoperiod at 120 µmol/m2s for 2 weeks until cotyledons appeared.
Plasmid construction and generation of transgenic hairy roots
All PCR amplifications used for plasmid construction were performed with Recombinant Taq DNA polymerase (THERMO SCIENTIFIC, Paisley, UK) according to the manufacturer’s instructions. To amplify LOX1.2, AOS1, OPR2 and αDOX1 promoter regions, tomato genomic DNA was used as a template for PCR, with the following primers: LOX1.2 FOR: 5′-TAT CAT ATA AGT GAG CTC GGA CTT ACT-3′, REV: 5′-AGC TGA CTG GCC CGG GTT TTC CTC AGA AAA AGT TTC-3′, with SacI and SmaI restriction sites; AOS1 FOR: 5′-CGT TTT CAC AGG TCG AAT TCA ACG CCG T-3′, REV: 5′-AGG TAC CTA GCC CGG GTT CTA TTA GAA AAA AAT CAA-3′, with EcoRI and SmaI restriction sites; OPR2 FOR: 5′-CTT TTA TGA ATG GTG GTA CCC TTT CCA-3′, REV: 5′-AGG TAC CTA GCC CGG GAC TTG ACA ACT AAA A-3′, with Kpn1 and SmaI restriction sites; αDOX1 FOR:5′-TTG GGA GAG AGG AGC TCG ACA ATT TTT-3′, REV-5′-AGG TAC CTA GCC CGG GTG TTT ATA CGA-3′, with SacI and SmaI restriction sites; all for ≈ 1500 pb amplicons. LOX1.2, AOS1, OPR2 and αDOX1 promoters were then cloned into the pUC19_Y vector122. The whole cassettes containing the specific gene promoters and the GUS reporter gene were then isolated by restriction digestion with SacI, EcoRI, KpnI and SacI (respectively) and SalI, to be cloned into the pCAMBIA2300 binary vector. The identity, orientation, and junctions of the resulting constructs pLOX1.2:GUS, pAOS1:GUS, pOPR2:GUS and pαDOX:GUS were confirmed by their digestion patterns. The pCAMBIA2300 empty-vector control and the four constructs were subsequently used for Rhizobium rhizogenes-mediated transformation123.
Rhizobium rhizogenes-mediated root transformation
R. rhizogenes ATCC 15,834 strain was used for the transformation by heat-shock method124. Individual cotyledons were excised from 15- to 20-day-old tomato seedlings grown as described above and immersed in a 2-day-old R. rhizogenes suspension for incubation at 28 °C for 2 h, with agitation at 100 rpm. The excised cotyledons then were placed on standard-strength Gamborg’s B5 salt media for 3 days for co-cultivation, and then transferred to B5 agar media supplemented with the antibiotics kanamycin (50 mg/mL) (DUCHEFA, Haarlem, the Netherlands) and timentin (15:1) at 300 mg/mL (DUCHEFA, Haarlem, the Netherlands). After 7–10 days of incubation in the dark at 25 °C, roots emerged from the wounded surface of the cotyledons. Hairy roots were transferred to Gamborg’s B5 medium containing 0.8% Gelrite and kanamycin (50 mg/mL). For nematode-infection experiments, transformed roots were subcultured in antibiotic-free media for 2 weeks, and 300 freshly hatched sterile M. javanica juveniles were used to inoculate the transgenic root lines, and root samples were taken at the designated time points for GUS assessment.
GUS bioassay
Two-week-old hairy root lines carrying the promoter GUS constructs were inoculated as described by Chinnapandi et al.125, and assayed histochemically for GUS activity at the designated times after infection with 300 sterile freshly hatched pre-parasitic M. javanica J2.
Plant oxylipin and hormone extraction
Tomato (Solanum lycopersicon cv. Avigail 870) seeds were sterilized and planted as described. Emerging roots were cut into 2-cm long segments, subcultured on Gamborg’s B5 medium and kept in the dark for 2 weeks. Root systems were inoculated with freshly hatched M. javanica J2 and galls were collected 5, 15 and 28 DAI. The noninoculated roots were collected as controls. Five independent biological replicates with a total of 100–130 mg of gall samples from inoculated and noninoculated tomato roots at the selected time points were collected into a 1.5-mL tube and kept at − 80 °C until further analysis. Samples were weighed and all of the data were normalized to the relative weight of those frozen tissues.
Oxylipins/hormones were extracted from each sample in liquid N2 using the phytohormone-extraction protocol reported by Yang et al.126 with the following modifications: 500 µL of phytohormone extraction buffer (1-propanol:water:HCl at 2:1:0.002 v/v) containing 500 nM deuterated internal standards: d-ABA ([2H6]( +)-cis, trans-ABA; [OLCHEM]), d-IAA ([2H5] indole-3-acetic acid, OLCHEM), d-JA (2,4,4-d3; acetyl-2,2-d2 JA; CDN Isotopes), and d-SA (d6-SA, SIGMA) were added to 100–130 mg gall tissue127. The gall tissue was homogenized at 6000 rpm for 30 s, twice. Samples were agitated for 30 min at 4 °C in the dark and then 500 µL dichloromethane was added and samples were agitated again for 30 min at 4 °C in the dark. Samples were centrifuged at 13,000 g for 5 min and the lower layer was collected into a glass vial for complete evaporation under a N2 gas stream. Samples were resuspended in 150 µL methanol, shaken for 1 min and then centrifuged in a 1.5-mL microcentrifuge tube at 14,000 g for 2 min to pellet any debris. Supernatant (100 µL) was collected into an autosampler vial for injection into a SCIEX API 3200 LC–MS/MS with a C18 column for chromatography and electrospray ionization. Peaks were integrated using Analyst 1.6.2 software and metabolites were quantified against internal standards41,127. Identification of fatty acid peaks was verified by comparison of the mass spectra to authentic standards. Noninoculated tomato roots served as controls.
Real-time qPCR analysis
For the qRT-PCR experiments, we removed contaminating genomic DNA from RNA with the Turbo DNA-Free Kit from AMBION (APPLIED BIOSYSTEMS). DNA-free RNA (1 μg) was converted to first-strand cDNA using the Verso cDNA Synthesis Kit (ABGENE, Epsom, UK), and reactions were performed using ABsolute SYBR Green ROX Mix (ABGENE). Primers for qRT-PCR experiments were designed with Primer Express software (APPLIED BIOSYSTEMS, Table 1S). A total volume of 10 μL contained 3.4 µL cDNA, consisting of 1 × SYBR-Green ROX Mix (ABGENE) and 150 nM forward primer and 150 nM reverse primer subjected to real-time PCR (Rotor-Gene RG-3000, CORBETT RESEARCH) using 0.1 mL 4-tube strips & caps (AXYGEN, Union City, CA, USA). All PCR cycles began with 2 min at 50 °C, then 10 min at 95 °C, followed by 40 cycles of 10 s at 95 °C and 1 min at 60 °C. After the PCR, a melting curve was generated by gradually increasing the temperature to 95 °C to test for amplicon specificity. For qRT-PCR, a mixture of all cDNAs was used for all treatments as a template for calibration curves designed for each pair of primers. Each reaction was performed in triplicate and the results represent the mean of two independent biological experiments. Two reference genes, EF-1α (GenBank accession no. U94493.1) and 18S (GenBank accession no. AF442193.1), were used as endogenous controls for gene-expression analysis. Transcript levels were normalized for each sample with the geometric mean of the corresponding selected reference genes. All of the reference genes were confirmed to display minimal variation across the treatment and were the most stable reference genes from a set of tested genes in a given cDNA sample. Values were expressed as the increase or decrease in level relative to a calibration sample. A negative control PCR without cDNA template was also run to confirm the absence of nonspecific PCR products (NTC) No Template Control127. The same process was performed for qRT-PCR of the expression of the nematode effectors TAG lipase and MLT-10.
FISH
Freshly hatched pre-parasitic M. javanica J2 were exposed to 9-HOT diluted in MES buffer to a final concentration of 10 mM, or to 0.01 M MES buffer, for 3 h; all samples were washed with 0.01 M MES buffer. The FISH procedure followed the method of Sakurai et al.128 with slight modifications129. The J2 were dissected manually with a razor blade and transferred to Carnoy's fixative (chloroform:ethanol:glacial acetic acid, 6:3:1, v/v) and fixed overnight. The samples were then decolorized in 6% (v/v) hydrogen peroxide in ethanol for 2 h and hybridized overnight in hybridization buffer (20 mM Tris–HCl pH 8.0, 0.9 M NaCl, 0.01% w/v SDS, 30% v/v formamide) containing 10 pmol fluorescent probe/mL. Based on the transcriptome sequences of interest, DNA probes were designed using Primer Express 3.0.1 software and checked for specificity using BLASTn (NCBI); TAG lipase (nematode effector) Cy5 (5′-Cy5-AATTGATGTTCGTGCAGACCAT-3′) and MLT-10-like Cy5 (5′-Cy5′-AGACAAAAGGGTGCAGAACGA-3′) were used as probes to target M. javanica J2. The stained samples were submerged in hybridization buffer supplemented with DAPI (0.1 mg/mL in 1X PBS) and transferred to a slide with liquid blocker, covered, sealed with nail polish and viewed under a confocal microscope.Detection specificity was confirmed using M. javanica exposed to 0.01 M MES buffer only as a control.
References
Bird, D. M. Signaling between nematodes and plants. Curr. Opin. Plant Biol. 7, 372–376 (2004).
Trudgill, D. L. & Blok, V. C. Apomitic, polyphagous root knot nematodes: exceptionally successful and damaging biotrophic root pathogens. Annu. Rev. Phytopathol. 39, 53–77 (2001).
Blok, V. C., Jones, J. T., Phillips, M. S. & Trudgill, D. L. Parasitism genes and host range disparities in biotrophic nematodes: the conundrum of polyphagy versus specialisation. BioEssays 30, 249–259 (2008).
Nicol, J. M. et al. Current nematode threats to world agriculture. In Genomics and Molecular Genetics of Plant–Nematode Interactions 21–43 (Springer, Dordrecht, 2011). https://doi.org/10.1007/978-94-007-0434-3_2.
Singh, S., Singh, B. & Singh, A. P. Nematodes: a threat to sustainability of agriculture. Procedia Environ. Sci. 29, 215–216 (2015).
Tesleem, T., Fawole, B. & Claudius-Cole, A. Management of root-knot nematodes (Meloidogyne Spp) on tomato using antagonistic plants. J. Biol. Agric. Healthc. 4, 97–100 (2014).
Jaouannet, M. & Rosso, M.-N. Effectors of root sedentary nematodes target diverse plant cell compartments to manipulate plant functions and promote infection. Plant Signal. Behav. 8, 1–5 (2013).
Haegeman, A., Mantelin, S., Jones, J. T. & Gheysen, G. Functional roles of effectors of plant-parasitic nematodes. Gene 492, 19–31 (2012).
Vieira, P. et al. The plant apoplasm is an important recipient compartment for nematode secreted proteins. J. Exp. Bot. 62, 1241–1253 (2011).
Davies, K. G. & Curtis, R. H. C. Cuticle surface coat of plant-parasitic nematodes. Annu. Rev. Phytopathol. 49, 135–156 (2011).
Bellafiore, S. et al. Direct identification of the Meloidogyne incognita secretome reveals proteins with host cell reprogramming potential. PLoS Pathog. 4, e1000192 (2008).
Robertson, L. et al. Cloning, expression and functional characterisation of a peroxiredoxin from the potato cyst nematode Globodera rostochiensis. Mol. Biochem. Parasitol. 111, 41–49 (2000).
Niu, J. et al. Msp40 effector of root-knot nematode manipulates plant immunity to facilitate parasitism. Sci. Rep. 6, 19443 (2016).
Doyle, E. A. & Lambert, K. N. Meloidogyne javanica chorismate mutase 1 alters plant cell development. Mol. Plant-Microbe Interact. 16, 123–131 (2003).
Postma, W. J. et al. The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants. PLANT Physiol. 160, 944–954 (2012).
Hamamouch, N. et al. The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism. J. Exp. Bot. 63, 3683–3695 (2012).
Molinari, S. & Miacola, C. Interactions between resistant tomato CVS and Meloidogyne spp. in vitro. Nematol. Mediteranea 25, 63–71 (1997).
Jaubert, S. et al. Direct identification of stylet secreted proteins from root-knot nematodes by a proteomic approach. Mol. Biochem. Parasitol. 121, 205–211 (2002).
Dubreuil, G., Magliano, M., Deleury, E., Abad, P. & Rosso, M. N. Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism. New Phytol. 176, 426–436 (2007).
Wang, X. Lipid signaling. Curr. Opin. Plant Biol. 7, 329–336 (2004).
Okazaki, Y. & Saito, K. Roles of lipids as signaling molecules and mitigators during stress response in plants. Plant J. 79, 584–596 (2014).
Prior, A. et al. A surface-associated retinol- and fatty acid-binding protein (Gp-FAR-1) from the potato cyst nematode Globodera pallida: lipid binding activities, structural analysis and expression pattern. Biochem. Soc. 356, 387–394 (2001).
Garofalo, A. The FAR protein family of the nematode Caenorhabditis elegans. Differential lipid binding properties, structural characteristics and developmental regulation. J. Biol. Chem. 278, 8065–8074 (2003).
Garofalo, A. et al. The FAR proteins of filarial nematodes: secretion, glycosylation and lipid binding characteristics. Mol. Biochem. Parasitol. 122, 161–170 (2002).
Ozalvo, R. et al. Two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, reveal distinct functions in response to plant-parasitic nematode infection. Mol. Plant Pathol. 15, 319–332 (2014).
Iberkleid, I. et al. Fatty acid-and retinol-binding protein, Mj-FAR-1 induces tomato host susceptibility to root-knot nematodes. PLoS ONE 8, e64586 (2013).
Kachroo, A. & Kachroo, P. Fatty acid-derived signals in plant defense. Annu. Rev. Phytopathol. 47, 153–176 (2009).
Farmer, E. E., Alméras, E. & Krishnamurthy, V. Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr. Opin. Plant Biol. 6, 372–378 (2003).
Mosblech, A., Feussner, I. & Heilmann, I. Oxylipins: structurally diverse metabolites from fatty acid oxidation. Plant Physiol. Biochem. 47, 511–517 (2009).
Chen, G. et al. Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds. Plant Physiol. 136, 2641–2651 (2004).
Howe, G. A. & Schilmiller, A. L. Oxylipin metabolism in response to stress. Curr. Opin. Plant Biol. 5, 230–236 (2002).
Griffiths, G. Biosynthesis and analysis of plant oxylipins. Free Radic. Res. https://doi.org/10.3109/10715762.2014.1000318 (2015).
Ballaré, C. L. Jasmonate-induced defenses: a tale of intelligence, collaborators and rascals. Trends Plant Sci. 16, 249–257 (2011).
Okada, K., Abe, H. & Arimura, G. I. Jasmonates induce both defense responses and communication in monocotyledonous and dicotyledonous plants. Plant Cell Physiol. 56, 16–27 (2015).
De León, I. P., Hamberg, M. & Castresana, C. Oxylipins in moss development and defense. Front. Plant Sci. 6, 1–12 (2015).
Garcia-Marcos, A., Pacheco, R., Manzano, A., Aguilar, E. & Tenllado, F. Oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato virus X-potato virus Y and tomato spotted wilt virus. J. Virol. 87, 5769–5783 (2013).
Andersson, M. X. et al. Oxylipin profiling of the hypersensitive response in Arabidopsis thaliana: formation of a novel oxo-phytodienoic acid-containing galactolipid, arabidopside E. J. Biol. Chem. 281, 31528–31537 (2006).
Cowley, T. & Walters, D. Local and systemic effects of oxylipins on powdery mildew infection in barley. Pest Manag. Sci. 61, 572–576 (2005).
Feussner, I. & Wasternack, C. The lipoxygenase pathway. Annu. Rev. Plant Biol. 53, 275–297 (2002).
Vellosillo, T. et al. Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate lateral root development and defense responses through a specific signaling cascade. Plant Cell Online 19, 831–846 (2007).
Christensen, S. A. et al. Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. 112, 11407–11412 (2015).
Prost, I. Evaluation of the antimicrobial activities of plant oxylipins supports their involvement in defense against pathogens. Plant Physiol. 139, 1902–1913 (2005).
Kolomiets, M. V. et al. A leaf lipoxygenase of potato induced specifically by pathogen infection 1 (2000).
Hwang, I. S. & Hwang, B. K. The pepper 9-lipoxygenase gene CaLOX1 functions in defense and cell death responses to microbial pathogens 1[C][W][OA]. https://doi.org/10.1104/pp.109.147827 (2010).
Göbel, C. et al. Oxylipin profiling reveals the preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells. J. Biol. Chem. 276, 6267–6273 (2001).
Gheysen, G. & Fenoll, C. Gene expression in nematode feeding sites. Annu. Rev. Phytopathol. 40, 191–219 (2002).
Abad, P., Favery, B., Rosso, M.-N. & Castagnone-Sereno, P. Root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction. Mol. Plant Pathol. 4, 217–224 (2003).
Veronico, P. et al. A novel lipoxygenase in pea roots. Its function in wounding and biotic stress. Plant Physiol. https://doi.org/10.1104/pp.106.081679 (2006).
Alkharouf, N. W., Klink, V. P. & Matthews, B. F. Identification of Heterodera glycines (soybean cyst nematode [SCN]) cDNA sequences with high identity to those of Caenorhabditis elegans having lethal mutant or RNAi phenotypes. Exp. Parasitol. 115, 247–258 (2007).
Leone, A., Melillo, M. T. & Bleve-Zacheo, T. Lipoxygenase in pea roots subjected to biotic stress. Plant Sci. 161, 703–717 (2001).
Zacheo, G., Bleve-Zacheo, T. & Melillo, M. T. Biochemistry of Plant Defence Responses to Nematode Infection 201–213 (Springer, Dordrecht, 1997). https://doi.org/10.1007/978-94-011-5596-0_16.
Gao, X., Stumpe, M., Feussner, I. & Kolomiets, M. A novel plastidial lipoxygenase of maize (Zea mays) ZmLOX6 encodes for a fatty acid hydroperoxide lyase and is uniquely regulated by phytohormones and pathogen infection. Planta 227, 491–503 (2008).
Naor, N. et al. Tight regulation of allene oxide synthase (AOS) and allene oxide cyclase-3 (AOC3) promote Arabidopsis susceptibility to the root-knot nematode Meloidogyne javanica. Eur. J. Plant Pathol. 150, 149–165 (2018).
Bhattarai, K. K. et al. Tomato susceptibility to root-knot nematodes requires an intact jasmonic acid signaling pathway. Mol. Plant-Microbe Interact. 21, 1205–1214 (2008).
Soriano, I. R., Asenstorfer, R. E., Schmidt, O. & Riley, I. T. Inducible flavone in oats (Avena sativa) is a novel defense against plant-parasitic nematodes. Phytopathology 94, 1207–1214 (2004).
Soriano, I. R., Riley, I. T., Potter, M. J. & Bowers, W. S. Phytoecdysteroids: a novel defense against plant-parasitic nematodes. J. Chem. Ecol. 30, 885–1899 (2004).
Cooper, W. R., Jia, L. & Goggin, L. Effects of jasmonate-induced defenses on root-knot nematode infection of resistant and susceptible tomato cultivars. J. Chem. Ecol. 31, 1953–1967 (2005).
Nahar, K., Kyndt, T., De Vleesschauwer, D., Höfte, M. & Gheysen, G. The jasmonate pathway is a key player in systemically induced defense against root knot nematodes in rice. Plant Physiol. 157, 305–316 (2011).
Gleason, C., Leelarasamee, N., Meldau, D. & Feussner, I. OPDA Has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis. Front. Plant Sci. 7, 1–11 (2016).
Wittek, F. et al. Arabidopsis ENHANCED DISEASE SUSCEPTIBILITY1 promotes systemic acquired resistance via azelaic acid and its precursor 9-oxo nonanoic acid. J. Exp. Bot. 65, 5919–5931 (2014).
Göbel, C., Feussner, I., Hamberg, M. & Rosahl, S. Oxylipin profiling in pathogen-infected potato leaves. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1584, 55–64 (2002).
Kyndt, T. et al. Below-ground attack by the root knot nematode Meloidogyne graminicola predisposes rice to blast disease. Mol. Plant Microbe Interact. 30, 255–266 (2017).
Heitz, T., Bergey, D. R. & Ryan, C. A. A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate. Plant Physiol. 114, 1085–1093 (1997).
Sivasankar, S., Sheldrick, B. & Rothstein, S. J. Expression of allene oxide synthase determines defense gene activation in tomato. Plant Physiol. 122, 1335–1342 (2000).
Howe, G. A., Lee, G. I., Itoh, A., Li, L. & DeRocher, A. Cytochrome P450-dependent metabolism of oxylipins in tomato. Cloning and expression of allene oxide synthase and fatty acid hydroperoxide lyase. Plant Physiol. 123, 711–724 (2000).
Strassner, J. et al. Characterization and cDNA - microarray expression analysis of 12-oxophytodienoate reductase reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response. Plant J. 32, 585–601 (2002).
Chini, A. et al. An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis. Nat. Chem. Biol. 14, 171–178 (2018).
Tirajoh, A., Aung, T. S. T., McKay, A. B. & Plant, A. L. Stress-responsive alpha-dioxygenase expression in tomato roots. J. Exp. Bot. 56, 713–723 (2005).
Vicente, J. et al. Role of 9-lipoxygenase and α-dioxygenase oxylipin pathways as modulators of local and systemic defense. Mol. Plant 5, 914–928 (2012).
Avila, C. A., Arevalo-Soliz, L. M., Lorence, A. & Goggin, F. L. Expression of α-DIOXYGENASE 1 in tomato and arabidopsis contributes to plant defenses against aphids. Mol. Plant Microbe Interact. 26, 977–986 (2013).
Hamberg, M., Sanz, A., Rodriguez, M. J., Calvo, A. P. & Castresana, C. Activation of the fatty acid alpha-dioxygenase pathway during bacterial infection of tobacco leaves. Formation of oxylipins protecting against cell death. J. Biol. Chem. 278, 51796–51805 (2003).
Prior, A. et al. A surface-associated retinol- and fatty acid-binding protein (GP-FAR-1) from the potato cyst nematode Globodera pallida: lipid binding activities, structural analysis and expression pattern. Biochem. J. 356, 387–394 (2001).
Iberkleid, I., Sela, N. & Brown Miyara, S. Meloidogyne javanica fatty acid- and retinol-binding protein (Mj-FAR-1) regulates expression of lipid-, cell wall-, stress- and phenylpropanoid-related genes during nematode infection of tomato. BMC Genom. 16, 272 (2015).
Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120 (2014).
Blanc-Mathieu, R. et al. Hybridization and polyploidy enable genomic plasticity without sex in the most devastating plant-parasitic nematodes. PLoS Genet. 13, e1006777 (2017).
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).
Lê, S., Josse, J., Rennes, A. & Husson, F. FactoMineR: an R package for multivariate analysis. JSS J. Stat. Softw. 25, 1–18 (2008).
Sonnhammer, E. L. L., Von Heijne, G. & Krogh, A. A Hidden Markov Model for Predicting Transmembrane Helices in Protein Sequences (AAAI Press, Palo Alto, 1998).
Foyer, C. H. Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. Plant Cell Online 17, 1866–1875 (2005).
Zeng, L. et al. The mouse fused locus encodes axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell 90, 181–192 (1997).
Weber, H. Fatty acid-derived signals in plants. Trends Plant Sci. 7, 217–224 (2002).
Li-Beisson, Y. et al. Acyl-lipid metabolism. Arab. B 8, e0133 (2010).
Nielsen, H. Predicting secretory proteins with SignalP. In: Methods in Molecular Biology (Clifton, N.J.) 1611, 59–73 (2017).
Spiegel, Y., Inbar, J., Kahane, I. & Sharon, E. Carbohydrate-recognition domains on the surface of phytophagous nematodes. Exp. Parasitol. 80, 220–227 (1995).
Curtis, R. H. C. Plant parasitic nematode proteins and the host parasite interaction. Brief. Funct. Genom. Proteom. 6, 50–58 (2007).
Zhuo, K. A. et al. A Meloidogyne graminicola C-type lectin, Mg01965, is secreted into the host apoplast to suppress plant defence and promote parasitism. mol. Plant pathol. 20, 346–355 (2019).
Huang, X., Xu, C.-L., Chen, W.-Z., Chen, C. & Xie, H. Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis. Sci. Rep. https://doi.org/10.1038/s41598-017-05093-7 (2017).
Lozano-Torres, J. L. et al. Dual disease resistance mediated by the immune receptor Cf-2 in tomato requires a common virulence target of a fungus and a nematode. Proc. Natl. Acad. Sci. 109, 10119–10124 (2012).
Petitot, A. S. et al. Dual RNA-seq reveals Meloidogyne graminicola transcriptome and candidate effectors during the interaction with rice plants. Mol. Plant Pathol. 17, 860–874 (2016).
Vanholme, B. et al. Secretions of plant-parasitic nematodes: a molecular update. Gene 332, 13–27 (2004).
Lozano-Torres, J. L. et al. Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors. PLoS Pathog. 10, e1004569 (2014).
Davies, L. J., Zhang, L. & Elling, A. A. The Arabidopsis thaliana papain-like cysteine protease RD21 interacts with a root-knot nematode effector protein. Nematology 17, 655–666 (2015).
Ali, S. et al. Analysis of putative apoplastic effectors from the nematode, Globodera rostochiensis, and identification of an expansin-like protein that can induce and suppress host defenses. PLoS ONE 10, e0115042 (2015).
Roze, E. et al. Mining the secretome of the root-knot nematode Meloidogyne chitwoodi for candidate parasitism genes. Mol. Plant Pathol. 9, 1–10 (2008).
Davis, E. L., Haegeman, A. & Kikuchi, T. Degradation of the plant cell wall by nematodes. In Genomics and Molecular Genetics of Plant–Nematode Interactions (eds Jones, J. et al.) 255–272 (Springer, Dordrecht, 2011). https://doi.org/10.1007/978-94-007-0434-3_12.
Moraes Filho, R. M., Menezes, A. F. & Martins, L. S. S. In silico modeling and characterization of phytoparasitic nematodes translationally-controlled tumor proteins. Genet. Mol. Res. 16, 1–11 (2017).
De Boer, J. M. et al. Cloning of a putative pectate lyase gene expressed in the subventral esophageal glands of Heterodera glycines. J. Nematol. 34, 9–11 (2002).
Kudla, U. et al. Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis. Mol. Plant Pathol. 8, 293–305 (2007).
Huang, G. et al. Developmental expression and molecular analysis of two Meloidogyne incognita pectate lyase genes. Int. J. Parasitol. 35, 685–692 (2005).
Pogorelko, G. V. et al. Re-targeting of a plant defense protease by a cyst nematode effector. Plant J. https://doi.org/10.1111/tpj.14295 (2019).
Ledger, T. N., Jaubert, S., Bosselut, N., Abad, P. & Rosso, M. N. Characterization of a new β-1,4-endoglucanase gene from the root-knot nematode Meloidogyne incognita and evolutionary scheme for phytonematode family 5 glycosyl hydrolases. Gene 382, 121–128 (2006).
Goellner, M., Smant, G., De Boer, J. M., Baum, T. J. & Davis, E. L. Isolation of beta-1,4-endoglucanase genes from Globodera tabacum and their expression during parasitism. J. Nematol. 32, 154 (2000).
Caillaud, M.-C. et al. Root-knot nematodes manipulate plant cell functions during a compatible interaction. J. Plant Physiol. 165, 104–113 (2008).
Dinh, P. T. Y., Knoblauch, M. & Elling, A. A. Nondestructive imaging of plant-parasitic nematode development and host response to nematode pathogenesis. Phytopathology 104, 497–506 (2014).
Maier, T. R., Hewezi, T., Peng, J. & Baum, T. J. Isolation of whole esophageal gland cells from plant-parasitic nematodes for transcriptome analyses and effector identification. Mol. Plant Microbe Interact. 26, 31–35 (2013).
Mitchum, M. G. et al. Nematode effector proteins: an emerging paradigm of parasitism. New Phytol. 199, 879–894 (2013).
Xie, J. et al. A novel meloidogyne incognita effector Misp12 suppresses plant defense response at latter stages of nematode parasitism. Front. Plant Sci. 7, 1–12 (2016).
Lin, B. et al. A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system. New Phytol. 209, 1159–1173 (2016).
Jaouannet, M. et al. The root-knot nematode calreticulin Mi-CRT is a key effector in plant defense suppression. Mol. Plant Microbe Interact. 26, 97–105 (2013).
Hewezi, T. et al. The cyst nematode effector protein 10A07 targets and recruits host posttranslational machinery to mediate its nuclear trafficking and to promote parasitism in Arabidopsis. Plant Cell 27, 891–907 (2015).
Affeldt, K. J., Brodhagen, M. & Keller, N. P. Aspergillus oxylipin signaling and quorum sensing pathways depend on G protein-coupled receptors. Toxins (Basel) 4, 695–717 (2012).
Lahvic, J. L. et al. Specific oxylipins enhance vertebrate hematopoiesis via the receptor GPR132. Proc. Natl. Acad. Sci. U. S. A. 115, 9252–9257 (2018).
Hussey, S. R. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis. Rep. 57, 1025–1028 (1973).
Van Vuuren, R. J. & Woodward, B. The response of cassava cultivars to root-knot nematode infestation: an in vitro method. Euphytica https://doi.org/10.1023/A:1017524210671 (2001).
Sijmons, P. C., Grundler, F. M. W., von Mende, N., Burrows, P. R. & Wyss, U. Arabidopsis thaliana as a new model host for plant-parasitic nematodes. Plant J. https://doi.org/10.1111/j.1365-313X.1991.00245.x (1991).
Demidchik, V. & Tester, M. Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots. Plant Physiol. 128, 379–387 (2002).
He, F. Total RNA extraction from C. elegans. Bio-protocol 101, e47 (2011).
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357–359 (2012).
Li, B. & Dewey, C. N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform. 12, 323 (2011).
Oliveros, J. An interactive tool for comparing lists with Venn diagrams. BioinfoGP, CNB-CSIC.
Lombard, V., Golaconda Ramulu, H., Drula, E., Coutinho, P. M. & Henrissat, B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res. 42, D490–D495 (2014).
Vancanneyt, G., Schmidt, R., O’Connor-Sanchez, A., Willmitzer, L. & Rocha-Sosa, M. Construction of an intron-containing marker gene: splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol. Gen. Genet. 220, 245–250 (1990).
Remy, S. et al. Improved T-DNA vector for tagging plant promoters via high-throughput luciferase screening. Biotechniques 38, 763–770 (2005).
Jiménez-Antaño, J. M., Pérez-Vargas, J., Ariza-Castolo, A., Guzmán, O. G. & Calva, G. C. An efficient heat-shock protocol for transformation of Agrobacterium rhizogenes without spontaneous generation to antibiotic resistance. Plant Omics https://doi.org/10.21475/poj.11.01.18.pne918 (2018).
Chinnapandi, B., Bucki, P. & Braun Miyara, S. SlWRKY45, nematode-responsive tomato WRKY gene, enhances susceptibility to the root knot nematode; M. javanica infection. Plant Signal. Behav. https://doi.org/10.1080/15592324.2017.1356530 (2017).
Yang, Q. et al. A gene encoding maize caffeoyl-CoA O-methyltransferase confers quantitative resistance to multiple pathogens. Nat. Genet. 49, 1364–1372 (2017).
Christensen, S. A. et al. The maize lipoxygenase, ZmLOX10, mediates green leaf volatile, jasmonate and herbivore-induced plant volatile production for defense against insect attack. Plant J. 74, 59–73 (2013).
Sakurai, M., Koga, R., Tsuchida, T., Meng, X.-Y. & Fukatsu, T. Rickettsia symbiont in the pea aphid Acyrthosiphon pisum: novel cellular tropism, effect on host fitness, and interaction with the essential symbiont Buchnera. Appl. Environ. Microbiol. 71, 4069–4075 (2005).
Yoon, D. S., Pendergrass, D. L. & Lee, M. H. A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline. MethodsX 3, 378–385 (2016).
Ogata, H., Goto, S., Fujibuchi, W. & Kanehisa, M. Computation with the KEGG pathway database. BioSystems 47, 119–128 (1998).
Acknowledgements
We thank the United States–Israel Binational Agricultural Research and Development, BARD Graduate Student Fellowship awarded to Nathalia Fitoussi, which enabled her to travel to Michael V. Kolomiets laboratory at Texas A&M to conduct all oxylipin metabolomics analyses. All authors would like to acknowledge the critical support of the Ministry of Agriculture and Rural Development of Israel, Award Number: 20-02-0110 and the United States–Israel Binational Agricultural Research and Development Fund, Award Number: IS-5184-19R.
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S.B., N.F., E.B., M.K., conceived and designed the experiment. N.F. and N.S. isolated and acquired the RNA-Seq data. N.F. and N.S. performed the transcriptomic alignment with the M. javanica genome. N.F., E.B. and M.K. performed the oxylipin analysis. N.F. and S.B. wrote the manuscript. E.B. performed all of the confocal microscopy work. P.B. and X.Q. helped with the data analysis. All authors made substantial contributions to the final text. All authors read and approved the final manuscript.
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Fitoussi, N., Borrego, E., Kolomiets, M.V. et al. Oxylipins are implicated as communication signals in tomato–root-knot nematode (Meloidogyne javanica) interaction. Sci Rep 11, 326 (2021). https://doi.org/10.1038/s41598-020-79432-6
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DOI: https://doi.org/10.1038/s41598-020-79432-6
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