Article
|
Open Access
Featured
-
-
Article
| Open Access
nextPYP: a comprehensive and scalable platform for characterizing protein variability in situ using single-particle cryo-electron tomography
nextPYP is a turn-key framework for single-particle cryo-electron tomography that streamlines complex data analysis pipelines, from pre-processing of tilt series to high-resolution refinement, for efficient analysis and visualization of large datasets.
- Hsuan-Fu Liu
- , Ye Zhou
- & Alberto Bartesaghi
-
Brief Communication
| Open Access
High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
For EM-based connectomics applications, a staining protocol for large tissue samples in the range of a centimeter has been developed, which avoids artifacts common with established protocols.
- Kun Song
- , Zhihui Feng
- & Moritz Helmstaedter
-
Comment |
Volume EM: a quiet revolution takes shape
Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning grassroots community effort is fast building the profile and revealing the impact of vEM technology in the life sciences and clinical research.
- Lucy M. Collinson
- , Carles Bosch
- & Paul Verkade
-
-
News & Views |
Mapping of the zebrafish brain takes shape
The generation of a whole larval zebrafish brain electron microscopy volume in tandem with automated tools lays the groundwork for producing the first vertebrate brain connectome.
- Paul Brooks
- , Andrew Champion
- & Marta Costa
-
Article |
FlyWire: online community for whole-brain connectomics
FlyWire is an online community and a platform for proofreading electron microscopy-based connectome data of the Drosophila brain.
- Sven Dorkenwald
- , Claire E. McKellar
- & H. Sebastian Seung
-
-
Brief Communication |
mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM
mEosEM survives 1% OsO4 treatment and Epon embedding for improved super-resolution correlative light and electron microscopy.
- Zhifei Fu
- , Dingming Peng
- & Pingyong Xu
-
Perspective |
Software tools for automated transmission electron microscopy
Py-EM and SerialEM enable automated microscope control for high-throughput data acquisition in diverse transmission electron microscopy imaging experiments.
- Martin Schorb
- , Isabella Haberbosch
- & David N. Mastronarde
-
-
Methods in Brief |
Zooming in on single tumor cells
-
Research Highlights |
Simple tools for difficult imaging
Small chemical tweaks during tissue preparation make a big difference for imaging thick, complex biological samples.
- Natalie de Souza
-
Review Article |
Focused ion beams in biology
Applications of focused ion beams for biological ultrastructure and chemical imaging, with a particular emphasis on the coupling of this technology with scanning electron microscopy, are discussed in this Review.
- Kedar Narayan
- & Sriram Subramaniam
-
Technology Feature |
Mapping proteins with spatial proteomics
A number of techniques address the location of proteins within cells.
- Vivien Marx
-
Brief Communication |
Fixation-resistant photoactivatable fluorescent proteins for CLEM
The fluorescent proteins mEos4a and mEos4b maintain their fluorescence and photoconversion after fixation with osmium. This property enables applications such as correlative super-resolution and electron microscopy.
- Maria G Paez-Segala
- , Mei G Sun
- & Loren L Looger
-
Brief Communication |
Directed evolution of APEX2 for electron microscopy and proximity labeling
A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels.
- Stephanie S Lam
- , Jeffrey D Martell
- & Alice Y Ting
-
Brief Communication |
Correlative super-resolution fluorescence and metal-replica transmission electron microscopy
A method for accurate correlative fluorescence and electron microscopy at nanometer resolution is reported for imaging structures at the cell surface.
- Kem A Sochacki
- , Gleb Shtengel
- & Justin W Taraska
-
Perspective |
Cellular-resolution connectomics: challenges of dense neural circuit reconstruction
Neuronal networks are high-dimensional graphs that are packed into three-dimensional nervous tissue at extremely high density. Comprehensively mapping these networks is therefore a major challenge. Although recent developments in volume electron microscopy imaging have made data acquisition feasible for circuits comprising a few hundreds to a few thousands of neurons, data analysis is massively lagging behind. The aim of this Perspective is to summarize and quantify the challenges for data analysis in cellular-resolution connectomics and describe current solutions involving online crowd-sourcing and machine-learning approaches.
- Moritz Helmstaedter
-
Brief Communication |
Elastic volume reconstruction from series of ultra-thin microscopy sections
The authors describe a method for realigning images from serially sectioned biological specimens that minimizes the effect of artificial deformations in the alignment by applying global elastic constraints. The method is applied to transmission electron microscopy and array tomography image series and is made available through the Fiji platform.
- Stephan Saalfeld
- , Richard Fetter
- & Pavel Tomancak
-
Research Highlights |
Etch-a-cell
A milling technique affords researchers a high-resolution glimpse deep into the cell using cryoelectron tomography.
- Allison Doerr
-
Correspondence |
Recovery of intact DNA nanostructures after agarose gel–based separation
- Gaëtan Bellot
- , Mark A McClintock
- & William M Shih
Serial Lift-Out: sampling the molecular anatomy of whole organisms
Contrast enhancement for cryo-ET