Sequencing

Sequencing refers to the techniques used to determine the primary structure of an unbranched biopolymer (DNA, RNA, protein, carbohydrate) resulting in a symbolic linear depiction of the monomeric subunits which summarizes much of the atomic-level structure of the molecule.

Featured

News & Views | 19 January 2023

sortChIC enables detailed chromatin analysis of rare cell types

Nature Genetics, 1-2
News & Views | 09 January 2023

Protein identification using a digestion-free nanopore approach

Nature Biotechnology, 1-2
News & Views | 05 January 2023

Single-cell recording of cellular RNAs in bacteria

Nature Biotechnology, 1-2

Latest Research and Reviews

Research
27 January 2023 | Open Access

MME⁺ fibro-adipogenic progenitors are the dominant adipogenic population during fatty infiltration in human skeletal muscle

Using single nuclei and single cell RNA-seq, 3 FAP cell subpopulations are identified in human skeletal muscle, with an MME⁺ subset showing increased adipogenic potential under fatty infiltration conditions.
- Gillian Fitzgerald
- , Guillermo Turiel
- & Katrien De Bock
Communications Biology 6, 111
Research
25 January 2023 | Open Access

Utilisation of exome sequencing for muscular disorders in Thai paediatric patients: diagnostic yield and mutational spectrum
- Sarinya Summa
- , Chupong Ittiwut
- & Vorasuk Shotelersuk
Scientific Reports 13, 1376
Research
25 January 2023 | Open Access

Decision level integration of unimodal and multimodal single cell data with scTriangulate

Single-cell genomics has expanded to measure diverse molecular modalities within the same cell. Here the authors provide a computational framework called scTriangulate to integrate cluster annotations from diverse independent sources, algorithms, and modalities to define statistically stable populations.
- Guangyuan Li
- , Baobao Song
- & Nathan Salomonis
Nature Communications 14, 406
Research
25 January 2023 | Open Access

Topological identification and interpretation for single-cell gene regulation elucidation across multiple platforms using scMGCA

A major challenge in analyzing scRNA-seq data arises from challenges related to dimensionality and the prevalence of dropout events. Here the authors develop a deep graph learning method called scMGCA based on a graph-embedding autoencoder that simultaneously learns cell-cell topology representation and cluster assignments, outperforming other state-of-the-art models across multiple platforms.
- Zhuohan Yu
- , Yanchi Su
- & Xiangtao Li
Nature Communications 14, 400
Research
25 January 2023 | Open Access

HDF1, a novel flowering time regulator identified in a mutant suppressing sensitivity to red light reduced 1 early flowering
- Mikael Johansson
- , Alexander Steffen
- & Dorothee Staiger
Scientific Reports 13, 1404
Research
24 January 2023 | Open Access

Hepatitis C and HIV detection by blood RNA-sequencing in cohort of smokers
- Jarrett D. Morrow
- , Peter J. Castaldi
- & Craig P. Hersh
Scientific Reports 13, 1357

All Research & Reviews

News and Comment

News & Views | 19 January 2023

sortChIC enables detailed chromatin analysis of rare cell types

Current methods of chromatin analysis focus mainly on the most abundant cell types in a sample. We present a workflow that combines enrichment of rare cell types with high-resolution mapping of histone modifications, which enables us to study chromatin dynamics in rare stem and progenitor cell populations.

Nature Genetics, 1-2
Research Highlights | 13 January 2023

Fanconi anemia-associated signature in cancer
- Safia Danovi
Nature Genetics 55, 1
News | 13 January 2023

Dads older than mums since dawn of humanity, study suggests

Scientists used modern human DNA to estimate when new generations were born over 250,000 years — and the age of parents at conception.
- Freda Kreier
Nature
Comments & Opinion | 12 January 2023

A light switch for targeted genomics

The combination of microscopy, targeted illumination and single-cell sequencing is driving applications from direct evolution to spatial omics.
- Rita Strack
Nature Methods 20, 32
Comments & Opinion | 12 January 2023

Long-read sequencing in the era of epigenomics and epitranscriptomics

As long-read sequencing technologies continue to advance, the possibility of obtaining maps of DNA and RNA modifications at single-molecule resolution has become a reality. Here we highlight the opportunities and challenges posed by the use of long-read sequencing technologies to study epigenetic and epitranscriptomic marks and how this will affect the way in which we approach the study of health and disease states.
- Morghan C. Lucas
- & Eva Maria Novoa
Nature Methods 20, 25-29
Editorial | 12 January 2023

Method of the Year 2022: long-read sequencing

Long-read sequencing powers a more complete reading of genomic information.

Nature Methods 20, 1

All News & Comment

Sequencing

Featured

sortChIC enables detailed chromatin analysis of rare cell types

Protein identification using a digestion-free nanopore approach

Single-cell recording of cellular RNAs in bacteria

Latest Research and Reviews

MME⁺ fibro-adipogenic progenitors are the dominant adipogenic population during fatty infiltration in human skeletal muscle

Utilisation of exome sequencing for muscular disorders in Thai paediatric patients: diagnostic yield and mutational spectrum

Decision level integration of unimodal and multimodal single cell data with scTriangulate

Topological identification and interpretation for single-cell gene regulation elucidation across multiple platforms using scMGCA

HDF1, a novel flowering time regulator identified in a mutant suppressing sensitivity to red light reduced 1 early flowering

Hepatitis C and HIV detection by blood RNA-sequencing in cohort of smokers

News and Comment

sortChIC enables detailed chromatin analysis of rare cell types

Fanconi anemia-associated signature in cancer

Dads older than mums since dawn of humanity, study suggests

A light switch for targeted genomics

Long-read sequencing in the era of epigenomics and epitranscriptomics

Method of the Year 2022: long-read sequencing

Search

Quick links

Sequencing

Featured

Related Subjects

Latest Research and Reviews

News and Comment

Search

Quick links