Oligonucleotide probes articles from across Nature Portfolio

Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.

Latest Research and Reviews

Research
05 November 2022 | Open Access

FRET-FISH probes chromatin compaction at individual genomic loci in single cells

Chromatin compaction affects many nuclear processes yet compaction levels at individual genomic loci have been notoriously difficult to assess. Here, Ana Mota and co-authors from the Bienko-Crosetto Lab present FRET-FISH for probing chromatin compaction at selected loci in single cells.
- Ana Mota
- , Szymon Berezicki
- & Magda Bienko
Nature Communications 13, 6680
Research | 24 October 2022

ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ

ClampFISH 2.0 enables highly specific multiplexed signal amplification in RNA FISH. The approach was used to detect 10 RNA species that ranged in abundance in more than 1 million cells and is also applicable to tissue sections.
- Ian Dardani
- , Benjamin L. Emert
- & Arjun Raj
Nature Methods 19, 1403-1410
Research
10 February 2022 | Open Access

Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH
- Ana Mota
- , Maud Schweitzer
- & Magda Bienko
Scientific Data 9, 47
Research
07 October 2021 | Open Access

Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins
- Cassandra M. Stawicki
- , Torri E. Rinker
- & Parag Mallick
Scientific Reports 11, 19921
Research
02 June 2021 | Open Access

Confirming putative variants at ≤ 5% allele frequency using allele enrichment and Sanger sequencing
- Yan Helen Yan
- , Sherry X. Chen
- & David Yu Zhang
Scientific Reports 11, 11640
Research
02 March 2021 | Open Access

Improved resolution in single-molecule localization microscopy using QD-PAINT
- Yeonho Chang
- , Do-Hyeon Kim
- & Sung Ho Ryu
Experimental & Molecular Medicine 53, 384-392

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News and Comment

Research Highlights | 04 February 2021

DNA-PAINT takes a left turn

DNA-PAINT with left-handed DNA oligomers enables imaging of nuclear structures with reduced background.
- Rita Strack
Nature Methods 18, 122
Research Highlights | 30 July 2015

A DNA-based sensor for intracellular chloride

Nature Methods 12, 708
Research Highlights | 31 March 2015

Stable linear probes for cellular mRNA labeling

Nature Methods 12, 288
Research Highlights | 29 May 2014

Cellular imaging of single-splice variants

Nature Methods 11, 609
Research Highlights | 28 March 2014

FISHing for faster findings

Quick-hybridizing probes help scientists image the high-speed events leading up to gene transcription.
- Michael Eisenstein
Nature Methods 11, 366-367
News & Views | 29 April 2013

Inner-outer beauty: DNA-binding surface tags as cellular barcodes

Zinc-finger DNA-binding domains are expressed on the surface of cells so that the cells can be tagged with fluorescently labeled oligonucleotides, enabling many possible applications in complex cell populations.
- Garry Nolan
Nature Methods 10, 399-401

All News & Comment

Oligonucleotide probes articles from across Nature Portfolio

Latest Research and Reviews

FRET-FISH probes chromatin compaction at individual genomic loci in single cells

ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ

Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH

Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins

Confirming putative variants at ≤ 5% allele frequency using allele enrichment and Sanger sequencing

Improved resolution in single-molecule localization microscopy using QD-PAINT

News and Comment

DNA-PAINT takes a left turn

A DNA-based sensor for intracellular chloride

Stable linear probes for cellular mRNA labeling

Cellular imaging of single-splice variants

FISHing for faster findings

Inner-outer beauty: DNA-binding surface tags as cellular barcodes

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