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| Open AccessShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement
- Hideji Murakoshi
- & Akihiro C. E. Shibata
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| Open AccessTowards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells
- Shingo Kikuta
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| Open AccessHigh-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment
- Wen Wan
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- & Jiong Hong
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| Open AccessAn engineered protein antagonist of K-Ras/B-Raf interaction
- Monique J. Kauke
- , Michael W. Traxlmayr
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| Open AccessDNA binding strength increases the processivity and activity of a Y-Family DNA polymerase
- Jing Wu
- , Alexandra de Paz
- & Keith E. J. Tyo
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| Open AccessA novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production
- José Manuel Borrero-de Acuña
- , Cristian Hidalgo-Dumont
- & Ignacio Poblete-Castro
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| Open AccessA thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
- Xiaojing Jia
- , Ying Liu
- & Yejun Han
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| Open AccessSemi-rational engineering of a thermostable aldo–keto reductase from Thermotoga maritima for synthesis of enantiopure ethyl-2-hydroxy-4-phenylbutyrate (EHPB)
- Zhiguo Wang
- , Shuo Zhou
- & Xiaoling Xu
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| Open AccessThe role of Antibody Vκ Framework 3 region towards Antigen binding: Effects on recombinant production and Protein L binding
- Chinh Tran-To Su
- , Wei-Li Ling
- & Samuel Ken-En Gan
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| Open AccessEffect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli
- Antti Kulmala
- , Tuomas Huovinen
- & Urpo Lamminmäki
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| Open AccessImpact of Module-X2 and Carbohydrate Binding Module-3 on the catalytic activity of associated glycoside hydrolases towards plant biomass
- Nandita Pasari
- , Nidhi Adlakha
- & Syed Shams Yazdani
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| Open AccessAutotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides
- Xianghai Cai
- , Wei Wang
- & Dongzhi Wei
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| Open AccessDevelopment of a selective agonist for relaxin family peptide receptor 3
- Dian Wei
- , Meng-Jun Hu
- & Zhan-Yun Guo
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| Open AccessSortase A-mediated crosslinked short-chain dehydrogenases/reductases as novel biocatalysts with improved thermostability and catalytic efficiency
- Kunpeng Li
- , Rongzhen Zhang
- & Rong Xiao
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| Open AccessA semi high-throughput method for screening small bispecific antibodies with high cytotoxicity
- Aruto Sugiyama
- , Mitsuo Umetsu
- & Izumi Kumagai
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| Open AccessConstruction of novel repeat proteins with rigid and predictable structures using a shared helix method
- Suk-Jun Youn
- , Na-Young Kwon
- & Jie-Oh Lee
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| Open AccessPrecise genome-wide base editing by the CRISPR Nickase system in yeast
- Atsushi Satomura
- , Ryosuke Nishioka
- & Mitsuyoshi Ueda
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| Open AccessDesign and evaluation of EphrinA1 mutants with cerebral protective effect
- Yuanjun Zhu
- , Yuanqing Gao
- & Yinye Wang
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| Open AccessCharacterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator
- Erik K. R. Hanko
- , Nigel P. Minton
- & Naglis Malys
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| Open AccessFRET-based Visualization of PDGF Receptor Activation at Membrane Microdomains
- Jihye Seong
- , Min Huang
- & Yingxiao Wang
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| Open AccessStructure aided design of a Neu5Gc specific lectin
- Christopher J. Day
- , Adrienne W. Paton
- & Michael P. Jennings
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| Open AccessDesign and application of a lactulose biosensor
- Jieyuan Wu
- , Peixia Jiang
- & Shuang-Yan Tang
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| Open AccessTargeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody
- Shahir S. Rizk
- , Somnath Mukherjee
- & Anthony A. Kossiakoff
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| Open AccessRational mutagenesis by engineering disulphide bonds improves Kluyveromyces lactis beta-galactosidase for high-temperature industrial applications
- Agustín Rico-Díaz
- , María-Efigenia Álvarez-Cao
- & Manuel Becerra
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| Open AccessEngineering Structurally Interacting RNA (sxRNA)
- Francis Doyle
- , Sameer Lapsia
- & Scott A. Tenenbaum
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| Open AccessA Novel Biocontainment Strategy Makes Bacterial Growth and Survival Dependent on Phosphite
- Ryuichi Hirota
- , Kenji Abe
- & Akio Kuroda
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| Open AccessRational Design of Protein C Activators
- Sergio Barranco-Medina
- , Mary Murphy
- & Nicola Pozzi
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| Open AccessEngineered yeast with a CO2-fixation pathway to improve the bio-ethanol production from xylose-mixed sugars
- Yun-Jie Li
- , Miao-Miao Wang
- & Tian-Wei Tan
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| Open AccessAutocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin
- J. Bonnet
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- & A. M. Di Guilmi
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| Open AccessIn vivo evaluation of a novel format of a bivalent HER3-targeting and albumin-binding therapeutic affibody construct
- Tarek Z. Bass
- , Maria Rosestedt
- & Anna Orlova
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| Open AccessA simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix
- Sau-Ching Wu
- , Chris Wang
- & Sui-Lam Wong
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| Open AccessEngineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency
- Canfang Niu
- , Peilong Yang
- & Bin Yao
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| Open AccessA new approach to quantification of mAb aggregates using peptide affinity probes
- Crystal S. F. Cheung
- , Kyle W. Anderson
- & Illarion V. Turko
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| Open AccessEngineering a pH responsive pore forming protein
- Matic Kisovec
- , Saša Rezelj
- & Gregor Anderluh
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| Open AccessTuning the allosteric regulation of artificial muscarinic and dopaminergic ligand-gated potassium channels by protein engineering of G protein-coupled receptors
- Christophe J. Moreau
- , Jean Revilloud
- & Michel Vivaudou
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| Open AccessTwo strategies to engineer flexible loops for improved enzyme thermostability
- Haoran Yu
- , Yihan Yan
- & Paul A. Dalby
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| Open AccessAn oxygen sensitive self-decision making engineered CAR T-cell
- Alexandre Juillerat
- , Alan Marechal
- & Laurent Poirot
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| Open AccessAntimicrobial Probiotics Reduce Salmonella enterica in Turkey Gastrointestinal Tracts
- Brittany Forkus
- , Seth Ritter
- & Yiannis N. Kaznessis
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| Open AccessSpontaneous Isopeptide Bond Formation as a Powerful Tool for Engineering Site-Specific Antibody-Drug Conjugates
- Vanessa Siegmund
- , Birgit Piater
- & Harald Kolmar
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| Open AccessMultivalent pIX phage display selects for distinct and improved antibody properties
- Lene S. Høydahl
- , Nicolay R. Nilssen
- & Geir Å. Løset
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| Open AccessA CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome
- Tianyuan Su
- , Fapeng Liu
- & Qingsheng Qi
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| Open AccessHarnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications
- Benjamin Rémy
- , Laure Plener
- & Eric Chabrière
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| Open AccessBeyond Antibodies: Development of a Novel Protein Scaffold Based on Human Chaperonin 10
- Abdulkarim M. Alsultan
- , David Y. Chin
- & Stephen M. Mahler
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| Open AccessDirect comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability
- Thu V. Vuong
- , Maryam Foumani
- & Emma R. Master
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| Open AccessAdenoviral vectors encoding CRISPR/Cas9 multiplexes rescue dystrophin synthesis in unselected populations of DMD muscle cells
- Ignazio Maggio
- , Jin Liu
- & Manuel A. F. V. Gonçalves
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| Open AccessAdenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines
- Ayami Kita
- , Nobumasa Hino
- & Takefumi Doi
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| Open AccessNear-infrared bioluminescent proteins for two-color multimodal imaging
- Konstantin A. Rumyantsev
- , Konstantin K. Turoverov
- & Vladislav V. Verkhusha
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| Open AccessMinicircle HBV cccDNA with a Gaussia luciferase reporter for investigating HBV cccDNA biology and developing cccDNA-targeting drugs
- Feng Li
- , Liang Cheng
- & Lishan Su
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| Open AccessA Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis
- Sylwia A. Mankowska
- , Pietro Gatti-Lafranconi
- & Florian Hollfelder