MHC

The major histocompatibility complex (MHC) is a cluster of genes encoding molecules that are important for lymphocyte activation. MHC molecules bind small fragments of proteins from inside a cell and present these at the cell surface. By scanning MHC molecules, lymphocytes can assess cells for signs of infection or damage.

Latest Research and Reviews

  • Research
    | Open Access

    Immunopeptidomics allows identifying the cellular repertoire of MHC-bound peptides, but quantifying them remains challenging. Here, the authors present a method to efficiently generate internal peptide MHC standards and calibration curves, facilitating relative and absolute quantitative immunopeptidomics.

    • Lauren E. Stopfer
    • , Joshua M. Mesfin
    • , Brian A. Joughin
    • , Douglas A. Lauffenburger
    •  & Forest M. White
  • Research
    | Open Access

    Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.

    • Sarah A. Overall
    • , Jugmohit S. Toor
    • , Stephanie Hao
    • , Mark Yarmarkovich
    • , Sara M. O’Rourke
    • , Giora I. Morozov
    • , Son Nguyen
    • , Alberto Sada Japp
    • , Nicolas Gonzalez
    • , Danai Moschidi
    • , Michael R. Betts
    • , John M. Maris
    • , Peter Smibert
    •  & Nikolaos G. Sgourakis
  • Research
    | Open Access

    Major Histocompatibility Complex (MHC) class I molecules present tightly binding peptides on the cell surface for recognition by cytotoxic T cells. Here, the authors present the crystal structures of a disulfide-stabilized human MHC class I molecule in the peptide-free state and bound with dipeptides, and find that peptide binding is accompanied by concerted conformational switches of the amino acid side chains in the binding pockets.

    • Raghavendra Anjanappa
    • , Maria Garcia-Alai
    • , Janine-Denise Kopicki
    • , Julia Lockhauserbäumer
    • , Mohamed Aboelmagd
    • , Janina Hinrichs
    • , Ioana Maria Nemtanu
    • , Charlotte Uetrecht
    • , Martin Zacharias
    • , Sebastian Springer
    •  & Rob Meijers
  • Research |

    Unanue and colleagues examine the immunopeptidome of pancreatic islets in non-obese diabetic (NOD) mice, which spontaneously develop autoimmune diabetes, to reveal the key features of a restricted component in the self-MHC-II peptidome that causes autoreactivity.

    • Xiaoxiao Wan
    • , Anthony N. Vomund
    • , Orion J. Peterson
    • , Alexander V. Chervonsky
    • , Cheryl F. Lichti
    •  & Emil R. Unanue
    Nature Immunology 21, 455-463
  • Research
    | Open Access

    The response to immunotherapy of melanoma patients is heterogeneous. Here, the authors demonstrate that a high expression of the two major components of the immunoproteasome, PSMB8 and PSMB9, modulates the production of HLA peptides and it is predictive of better survival and improved response to immune-checkpoint inhibitors of melanoma patients.

    • Shelly Kalaora
    • , Joo Sang Lee
    • , Eilon Barnea
    • , Ronen Levy
    • , Polina Greenberg
    • , Michal Alon
    • , Gal Yagel
    • , Gitit Bar Eli
    • , Roni Oren
    • , Aviyah Peri
    • , Sushant Patkar
    • , Lital Bitton
    • , Steven A. Rosenberg
    • , Michal Lotem
    • , Yishai Levin
    • , Arie Admon
    • , Eytan Ruppin
    •  & Yardena Samuels

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