Immunochemistry

An optimized method for mass spectrometry based immunopeptidomics analysis using high-field asymmetric waveform ion mobility spectrometry is presented and identifies 44,815 HLA-bound peptides (HLAp) from colorectal cancer samples.

Systematic measurements of the interactions between proteins found on the surfaces of human leukocytes provides a global view of the way that immune cells are dynamically connected by receptors.

Melatonin induces Parkin 1 dependent mitophagy in human umbilical vein endothelial cells through the AMPK/TFEB pathway, alleviating oxidative stress and apoptosis, which also enables skin flap survival in vivo.

Itaconate is an immunomodulatory macrophage metabolite. Mesaconate, a structurally similar molecule, is shown to be synthesized from itaconate in inflammatory macrophages and shows similar immunomodulatory effects, despite not repressing tricarboxylic acid cycle activity nor inhibiting succinate dehydrogenase activity.