Fluorescent labelling articles within Nature Chemistry

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• News & Views | 22 March 2012

  Playing tag with proteins

  Fluorescent labels can now be attached to a specific protein on the surface of live cells using a two-step method that reacts a norbornene — introduced using genetic encoding — with a variety of dyes.

  • Dante W. Romanini
  •  & Virginia W. Cornish

• Article | 05 February 2012

  Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

  The site-specific incorporation of a norbornene amino acid into proteins via genetic code expansion, together with the synthesis of a series of tetrazine-based probes that exhibit turn-on fluorescence on their fast cycloaddition with norbornene, enables rapid protein labelling on mammalian cells.

  • Kathrin Lang
  • , Lloyd Davis
  •  & Jason W. Chin