Cryoelectron microscopy articles from across Nature Portfolio

Cryoelectron microscopy is a method for imaging frozen-hydrated specimens at cryogenic temperatures by electron microscopy. Specimens remain in their native state without the need for dyes or fixatives, allowing the study of fine cellular structures, viruses and protein complexes at molecular resolution.

Featured

News & Views | 25 May 2023

Captured: the elusive eukaryotic tRNA splicing enzyme
- Anita K. Hopper
- & Jinwei Zhang
Nature Structural & Molecular Biology, 1-3
News & Views | 18 May 2023

A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution

Nature Structural & Molecular Biology, 1-2
News & Views | 12 May 2023

Mapping the motion and structure of flexible proteins from cryo-EM data

Nature Methods 20, 797-798

Latest Research and Reviews

Research
06 June 2023 | Open Access

Structural insights into ligand recognition and activation of the medium-chain fatty acid-sensing receptor GPR84

The orphan GPR84 plays important roles in inflammation, fibrosis, and metabolism. Here, authors report cryo-EM structures of the receptor bound to two ligands, with insights into ligand binding and entry from the extracellular milieu.
- Heng Liu
- , Qing Zhang
- & Wanchao Yin
Nature Communications 14, 3271
Research
03 June 2023 | Open Access

Improvement of cryo-EM maps by simultaneous local and non-local deep learning

Map post-processing is crucial for cryo-EM modeling building. Here, the authors present a deep learning approach to improve both the quality and interpretability of cryo-EM maps by simultaneously considering local and non-local effects.
- Jiahua He
- , Tao Li
- & Sheng-You Huang
Nature Communications 14, 3217
Research
03 June 2023 | Open Access

Structural mapping of Na_v1.7 antagonists

Voltage-gated sodium (Nav) channels are targeted by various clinically applied and investigational drugs. Here cryo-EM structures of Nav1.7 bound to 7 compounds with diverse chemical skeletons reveal the structural basis of action of these drugs and provide a 3D structural map for drug binding sites on Nav channels.
- Qiurong Wu
- , Jian Huang
- & Nieng Yan
Nature Communications 14, 3224
Research
01 June 2023 | Open Access

Reply to: The G protein preference of orexin receptors is currently an unresolved issue
- Jie Yin
- , Yanyong Kang
- & Daniel M. Rosenbaum
Nature Communications 14, 3163
Research | 01 June 2023

Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand

Using a combination of biochemical, structural and computational analyses, the authors unveil the mechanism of bacterial RNA polymerase pausing and restart mediated by a small-molecule-dependent RNA structural element termed a riboswitch.
- Adrien Chauvier
- , Jason C. Porta
- & Nils G. Walter
Nature Structural & Molecular Biology, 1-12
Research
01 June 2023 | Open Access

Structural interplay of anesthetics and paralytics on muscle nicotinic receptors

Here the authors use a structural approach to reveal how neuromuscular blockers and a general anesthetic antagonize the muscle-type nicotinic receptor through competitive and allosteric mechanisms.
- Umang Goswami
- , Md Mahfuzur Rahman
- & Ryan E. Hibbs
Nature Communications 14, 3169

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News and Comment

Research Highlights | 30 May 2023

The first step of hypusination
- Sophie McKenna
Nature Chemical Biology 19, 664
News & Views | 25 May 2023

Captured: the elusive eukaryotic tRNA splicing enzyme

The maturation of transfer RNAs requires the splicing of precursor tRNAs by specific endonucleases. New cryo-electron microscopy studies of the human splicing endonuclease bound to tRNAs shed light on how it cleaves and splices its substrates, explaining the function of eukaryote-specific enzyme subunits and rationalizing disease-associated mutations.
- Anita K. Hopper
- & Jinwei Zhang
Nature Structural & Molecular Biology, 1-3
Research Highlights | 23 May 2023

How “rock-and-roll” solved the cullin supply chain problem
- Sheena Faye Garcia
- & Michele Pagano
Cell Research, 1-2
News & Views | 18 May 2023

A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution

The correction of errors during mitochondrial DNA replication by the proofreading function of DNA polymerase-γ is crucial for maintaining the integrity of the mitochondrial genome and the production of cellular energy. Using cryo-electron microscopy, several steps of the DNA polymerase-γ proofreading pathway have been revealed at near-atomic resolution.

Nature Structural & Molecular Biology, 1-2
News & Views | 12 May 2023

Mapping the motion and structure of flexible proteins from cryo-EM data

A deep learning algorithm maps out the continuous conformational changes of flexible protein molecules from single-particle cryo-electron microscopy images, allowing the visualization of the conformational landscape of a protein with improved resolution of its moving parts.

Nature Methods 20, 797-798
Correspondence | 10 May 2023

Structure of the SNAPc-bound RNA polymerase III preinitiation complex
- Haifeng Hou
- , Qianwei Jin
- & Yanhui Xu
Cell Research, 1-4

All News & Comment

Cryoelectron microscopy articles from across Nature Portfolio

Featured

Captured: the elusive eukaryotic tRNA splicing enzyme

A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution

Mapping the motion and structure of flexible proteins from cryo-EM data

Latest Research and Reviews

Structural insights into ligand recognition and activation of the medium-chain fatty acid-sensing receptor GPR84

Improvement of cryo-EM maps by simultaneous local and non-local deep learning

Structural mapping of Na_v1.7 antagonists

Reply to: The G protein preference of orexin receptors is currently an unresolved issue

Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand

Structural interplay of anesthetics and paralytics on muscle nicotinic receptors

News and Comment

The first step of hypusination

Captured: the elusive eukaryotic tRNA splicing enzyme

How “rock-and-roll” solved the cullin supply chain problem

A look into the dynamic process of proofreading in DNA polymerase-γ at near-atomic resolution

Mapping the motion and structure of flexible proteins from cryo-EM data

Structure of the SNAPc-bound RNA polymerase III preinitiation complex

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