CRISPR-Cas systems

CRISPR-Cas systems are prokaryotic immune systems that confer resistance to foreign genetic elements such as plasmids and phages. CRISPR-Cas systems have been exploited for targeted genome editing.

Latest Research and Reviews

  • Research | | open

    Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine-resolution mapping.

    • Byungjin Hwang
    • , Wookjae Lee
    • , Soo-Young Yum
    • , Yujin Jeon
    • , Namjin Cho
    • , Goo Jang
    •  & Duhee Bang
  • Research | | open

    CRISPR-Cas9 has been rapidly adopted to generate cell line models of disease. Here the authors show, while attempting to establish a congenital erythropoietic porphyria model, unexpected chromosome truncations generated by a p53-dependent mechanism.

    • Grégoire Cullot
    • , Julian Boutin
    • , Jérôme Toutain
    • , Florence Prat
    • , Perrine Pennamen
    • , Caroline Rooryck
    • , Martin Teichmann
    • , Emilie Rousseau
    • , Isabelle Lamrissi-Garcia
    • , Véronique Guyonnet-Duperat
    • , Alice Bibeyran
    • , Magalie Lalanne
    • , Valérie Prouzet-Mauléon
    • , Béatrice Turcq
    • , Cécile Ged
    • , Jean-Marc Blouin
    • , Emmanuel Richard
    • , Sandrine Dabernat
    • , François Moreau-Gaudry
    •  & Aurélie Bedel
  • Research | | open

    Dimitre Simeonov, Alexander Brandt et al. report a pathogenic bystander mutation caused by unintended repair of a CRISPR-Cas9-mediated deletion in mice. They generate mice lacking an IL2RA intronic enhancer previously associated with human disease risk and find that one line of edited mice show unexpected disease features due to a bystander mutation.

    • Dimitre R. Simeonov
    • , Alexander J. Brandt
    • , Alice Y. Chan
    • , Jessica T. Cortez
    • , Zhongmei Li
    • , Jonathan M. Woo
    • , Youjin Lee
    • , Claudia M. B. Carvalho
    • , Alyssa C. Indart
    • , Theodore L. Roth
    • , James Zou
    • , Andrew P. May
    • , James R. Lupski
    • , Mark S. Anderson
    • , F. William Buaas
    • , Daniel S. Rokhsar
    •  & Alexander Marson
  • Research | | open

    Analogue regulation of gene expression is important for normal function in mammals but existing genetic technologies are designed to achieve ON/OFF control. Here the authors develop synthetic microRNA silencing-mediated fine-tuners (miSFITs) to precisely control target gene expression levels.

    • Yale S. Michaels
    • , Mike B. Barnkob
    • , Hector Barbosa
    • , Toni A. Baeumler
    • , Mary K. Thompson
    • , Violaine Andre
    • , Huw Colin-York
    • , Marco Fritzsche
    • , Uzi Gileadi
    • , Hilary M. Sheppard
    • , David J. H. F. Knapp
    • , Thomas A. Milne
    • , Vincenzo Cerundolo
    •  & Tudor A. Fulga

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