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Circadian rhythms are observable biological oscillations that occur with a 24-hour periodicity. They are based on an endogenous transcriptional clock, which itself is reinforced by environmental cues such as variations in light and temperature.
The plant circadian clock regulates numerous developmental processes such as seasonal growth and flowering time. Here Ritter et al. identify two transcription factors, FRS7 and FRS12, which interact to form a repressor complex that regulates clock output partially by controlling the expression of GIGANTEA and PIF4.
The plant-specific GIGANTEA protein regulates the circadian clock by stabilizing the F-box protein ZEITLUPE via an unknown mechanism. Here Cha et al. show that GIGANTEA has intrinsic chaperone activity and can facilitate ZEITLUPE maturation by acting synergistically with HSP90.
The PIF4 transcription factor mediates the response of Arabidopsis seedlings to elevated temperature. Here the authors show that PIF4 interacts with the circadian clock component TOC1 which acts to suppress the PIF4-mediated temperature response in the evening.
Endo et al. describe a protocol for the isolation of mesophyll, vascular and epidermal tissues from Arabidopsis leaves. The protocol can be applied to tissue-specific transcriptome, methylome and proteome studies in a number of crop plants.
The Arabidopsis CCA1 transcription factor is a core regulator of the circadian clock. Here, the authors show that the LWD1 protein, in complex with the TCP20 or TCP22 transcription factors, acts as a co-activator of CCA1 expression contributing to elevated CCA1 expression at dawn.