Chromosome conformation capture-based methods

Chromosome conformation capture (3C)-based methods reveal chromosome organization within the nucleus by determining the physical proximity of pairs of points along chromatin. They preserve chromatin interactions by cross-linking followed by fragmentation, ligation and sequencing of interacting regions. Genome organization affects processes such as transcription, repair and DNA replication.

Latest Research and Reviews

News and Comment

  • Research Highlights |

    Two studies in Molecular Cell report fine-scale structural profiles of mammalian genomes using Micro-C, indicating that fine chromosomal structure is regulated by diverse transcription-related features.

    • Darren J. Burgess
  • Research Highlights |

    A study in Nature provides new insights into chromatin dynamics and allele-specific gene expression regulation during early mouse embryogenesis.

    • Linda Koch
  • Research Highlights |

    A study in Nature Genetics shows that genomic rearrangements that cause extensive changes to chromatin topology do not alter expression for the majority of genes.

    • Linda Koch
  • Research Highlights |

    Independent efforts shine light on the 3D genome structure by looking at multiple contacts along an allele or equalizing the distance between restriction sites for higher-resolution Hi-C maps.

    • Nicole Rusk
    Nature Methods 15, 647