• Microscopic glass slides (Blue Star)
• Microscopic cover glasses (Blue Star, 22x50 mm)
• Mcllvaine’s buffer preparation
I. Stock solution A: 0.1 M citric acid solution. Dissolve 4.2 g of citric acid (Merck, Cat. No. 61799705001730) in 200 ml double distilled water
II. Stock solution B: 0.2 M Na2HPO4 solution. Dissolve 22.7 g of Na2HPO4 (Merck, Cat. No. 1.06559.0500) in 800 ml double distilled water
III. Mix 2 parts of stock solution A and 8 parts of stock solution B to make pH 7.0 buffer
• Formaldehyde (Merck, Cat No. 8.18708.1000)
• Triton-X-100 (HiMedia, Cat. No. MB031-100ML)
• 4', 6 - diamidino-2-phenylindoledihydrochloride (DAPI) solution preparation
I. Dissolve 2 mg DAPI (Sigma-Aldrich, Cat. No. D9542) in 1 ml Mcllvaine’s buffer to prepare a main stock solution of concentration of 2 mg/ ml. Store it in dark at - 20°C
II. Prepare working stock solution of the concentration of 20 µg/ ml by diluting 10 µl of the main stock solution with 990 µl of Mcllvaine’s buffer. Store it in dark at - 20°C