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Frozen in action: cryo-EM structure of a GPCR–G-protein complex

Nature Structural & Molecular Biology volume 24, pages 500–502 (2017)Cite this article

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Interaction with heterotrimeric G proteins is a hallmark of G-protein-coupled receptor (GPCR) family members, and it is the key step for a diverse range of cell-signaling cascades. A recent cryo-EM structure of the human calcitonin receptor (CTR) in complex with a G-protein heterotrimer reveals novel insights into receptor–G-protein coupling.

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Figure 1: Progress in the structural analysis of GPCR signaling complexes by EM.
Figure 2: Structural comparison of the β2AR–G-protein complex and the CTR–G-protein complex.
Figure 3: Superimposition of the structures of the β2AR–G-protein complex and the CTR–G-protein complex.

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Acknowledgements

The research program in A.K.S.'s laboratory is supported by the Wellcome Trust DBT India Alliance (IA/I/14/1/501285), the Department of Biotechnology (DBT), the Department of Science and Technology (DST), the Council for Scientific and Industrial Research (CSIR) and the Institute of Technology, Kanpur.

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  1. Hemlata Dwivedi and Arun K. Shukla are in the Department of Biological Sciences and Bioengineering, Mithu Baidya, Indian Institute of Technology, Kanpur, India.,

    Mithu Baidya, Hemlata Dwivedi & Arun K Shukla

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  1. Mithu Baidya
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Correspondence to Arun K Shukla.

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Baidya, M., Dwivedi, H. & Shukla, A. Frozen in action: cryo-EM structure of a GPCR–G-protein complex. Nat Struct Mol Biol 24, 500–502 (2017). https://doi.org/10.1038/nsmb.3418

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