PrimeViews in 2021

This PrimeView highlights some of the key steps of tissue clearing protocols and details some of the main considerations when clearing a sample for fluorescent imaging.

This PrimeView highlights the design and use of whispering-gallery-mode microcavities in biological and physical sensing.

This PrimeView highlights the best practices for using Raman microspectroscopy to study microorganisms, with a focus on enhancing reproducibility and data sharing.

This PrimeView highlights the use of powder diffraction to characterise crystalline structures.

This PrimeView on 3D extrusion bioprinting accompanies the Primer by Zhang et al. and highlights the main stages of the 3D extrusion bioprinting process.

This PrimeView describes the best practices for using light sheet fluorescence microscopy to image a range of sample types, with a special focus on light sheet properties and image detection.

This PrimeView highlights how sedimentary deposits may be dated using optically stimulated luminescence of quartz grains.

This PrimeView on adaptive optics highlights the general experimental setup of this technique that corrects image imperfections in astronomy, vision science and microscopy.

This PrimeView on small-angle X-ray and neutron scattering accompanies the Primer by Jeffries et al. and highlights the general experimental setup of this technique probeing the structural organisation of matter.

This PrimeView highlights the design, fabrication and use of electrolyte-gated field effect transistors.

This PrimeView on force spectroscopy of single cells using atomic force microscopy accompanies the Primer by Viljoen et al. and highlights the various applications enabled by this mechanical probing technique.

This PrimeView highlights the best practices for radiocarbon dating of various sample types. Improvements in sensitivity and accuracy allow for dating of rare and precious samples.

This PrimeView covers practical and ethical considerations for conducting a genome-wide association study (GWAS) and shows the key features of a Manhattan plot, a widely used scheme to study GWAS results.

This PrimeView highlights best practices for psychometric network analysis. Network topology types are discussed in relation to the type of data being collected.

This PrimeView describes a strategic workflow for studying N₂ activation methods, emphasizing the importance of verifying genuine product formation.

This PrimeView highlights the information on ubiquitin-like modifications and their protein targets that can be derived using proteomic techniques.

This PrimeView on atom probe tomography accompanies the Primer by Gault et al. and highlights the three-dimensional nature of this nanometre-scale compositional mapping technique.

This PrimeView highlights the steps involved in designing experiments for cell free gene expression. This technique has many benefits that make it suitable for a wide range of applications.

This PrimeView describes novel fluorination and fluoroalkylation techniques and the key considerations for their use, and highlights the range of applications for fluorinated molecules in society.

This PrimeView describes the factors that need to be considered when developing a biocatalyst and how they impact the utility of these enzymes in real-world applications.