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Regenerative rehabilitation is the combination of regenerative biology with approaches for physical medicine. Examples of regenerative rehabilitation include the incorporation of physical activity to promote engraftment of stem cells into muscle tissue and the in vitro use of mechanical stimuli on cultured cells or tissues, as a means to optimize the efficacy of cell therapeutics and tissue engineering technologies. This collection includes content from npj Regenerative Medicine and showcases the advances in technology and bioscaffolds and our understanding of how mechanical forces regulate gene expression, cell fate and cell function to use mechanical forces for regenerative medicine. This collection was coordinated by Associate Editor Dr. Thomas Rando, Guest Editor Dr. Fabrisia Ambrosio, and Managing Editor Dr. Marie-Elizabeth A. Barabas.