Volume 19

  • No. 9 September 2022

    Heat-stabilized antibodies for rapid deep tissue immunolabeling

    Heat-stabilized antibodies (SPEARS) enable thermally facilitated 3D immunolabeling (THiCK staining) of parvalbumin-expressing cells in a mouse cerebellar hemisphere.

    See Lai et al.

  • No. 8 August 2022

    Monitoring blood flow in the brain at high spatiotemporal resolution

    Functional ultrasound localization microscopy reveals whole-brain vascular changes during neuronal activation at high resolution, providing quantitative information on changes in flow, speed and vessel diameter in multiple vascular compartments over a wide field of view.

    See Renaudin et al.

  • No. 7 July 2022

    High-content 3D organoid imaging

    JeWell microchips facilitate compartmentalized organoid culture and allow single-objective light sheet imaging of up to 96 organoids in 3D and in three colors in one hour.

    See Beghin et al.

  • No. 6 June 2022

    Tools for assembling and analyzing complete genomes

    With new tools developed by the Telomere-to-Telomere (T2T) Consortium, the human genome is revealed in greater quality and detail.

    See Editorial

  • No. 5 May 2022

    Versatile multiscale imaging of cleared tissues

    On the cover, an optically cleared mouse brain imaged with a hybrid open-top light-sheet microscope.

    See Glaser et al.

  • No. 4 April 2022

    COVID-19 research: methods lead the way

    Decades of accumulated methods development across diverse areas of basic biological research have facilitated a speedy scientific response to the SARS-CoV-2 virus.

    See Editorial

  • No. 3 March 2022

    Tools and guidelines for multiplexed tissue imaging

    IBEX (iterative bleaching extends multiplexity) imaging of cell–cell interactions in a human lymph node evokes a stained glass window in a cathedral.

    See Hickey et al.

  • No. 2 February 2022

    Cryo-ExM preserves cellular ultrastructure

    A human cell in mitosis observed using cryo-expansion microscopy (Cryo-ExM). The DNA is stained pink and the rest of the cell with an NHS ester that marks the proteome and highlights the mitochondria in black at each pole of the mitotic spindle.

    See Laporte et al.

  • No. 1 January 2022

    Method of the Year 2021: Protein structure prediction

    Protein structure prediction is our Method of the Year 2021, for the recent development of deep-learning-based methods that predict structures with unprecedented accuracy. On the cover, a blizzard of protein structure models from the AlphaFold Protein Structure Database (https://alphafold.ebi.ac.uk/), predicted by the method AlphaFold2.

    See Editorial