Volume 17 Issue 6, June 2020

Volume 17 Issue 6

Targeted sequencing perturbations

The cover illustrates targeted Perturb-seq (TAP-seq), which uses a universal PCR handle and gene-specific primers to amplify genes of interest in single cells. TAP-seq boosts the scale and precision of functional genomics screens and uncovers enhancer–target gene interactions at genome scale.

See Schraivogel et al.

Image: illustratoren.de/TobiasWuestefeld. Cover design: Erin Dewalt.

Editorial

  • Editorial |

    We conducted an informal survey to learn how scientists are using Twitter. We share some of the responses and discuss why being active on Twitter offers real benefits.

This Month

  • This Month |

    A better base editor with fewer off-target changes, from a die-hard Manchester United fan.

    • Vivien Marx

Research Highlights

Technology Feature

Review Articles

  • Review Article |

    This Review discusses temporal focusing microscopy and its applications in neuroscience for imaging and optogenetic activation.

    • Eirini Papagiakoumou
    • , Emiliano Ronzitti
    •  & Valentina Emiliani

Matters Arising

Analysis

Brief Communications

  • Brief Communication |

    Sorting RT-FDC combines real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves to transfer molecular specificity to label-free, image-based cell sorting using an efficient deep neural network.

    • Ahmad Ahsan Nawaz
    • , Marta Urbanska
    • , Maik Herbig
    • , Martin Nötzel
    • , Martin Kräter
    • , Philipp Rosendahl
    • , Christoph Herold
    • , Nicole Toepfner
    • , Markéta Kubánková
    • , Ruchi Goswami
    • , Shada Abuhattum
    • , Felix Reichel
    • , Paul Müller
    • , Anna Taubenberger
    • , Salvatore Girardo
    • , Angela Jacobi
    •  & Jochen Guck
  • Brief Communication |

    Three-photon microscopy provides access to most of the adult zebrafish brain for both structural and functional imaging, as well as to the Danionella dracula brain.

    • Dawnis M. Chow
    • , David Sinefeld
    • , Kristine E. Kolkman
    • , Dimitre G. Ouzounov
    • , Najva Akbari
    • , Rose Tatarsky
    • , Andrew Bass
    • , Chris Xu
    •  & Joseph R. Fetcho

Articles

  • Article |

    Targeted sequencing of perturbation effects offers a sensitive approach to capture genes of interest in CRISPR-mediated screens, enabling genome-scale screens at higher scale and lower cost than whole-transcriptome Perturb-seq.

    • Daniel Schraivogel
    • , Andreas R. Gschwind
    • , Jennifer H. Milbank
    • , Daniel R. Leonce
    • , Petra Jakob
    • , Lukas Mathur
    • , Jan O. Korbel
    • , Christoph A. Merten
    • , Lars Velten
    •  & Lars M. Steinmetz
  • Article |

    CRISPR-based microraft followed by guide RNA identification (CRaft-ID) combines microraft arrays, microscopy and CRISPR–Cas9 technology for high-content image-based phenotyping. CRaft-ID was used to identify proteins involved in stress granule formation.

    • Emily C. Wheeler
    • , Anthony Q. Vu
    • , Jaclyn M. Einstein
    • , Matthew DiSalvo
    • , Noorsher Ahmed
    • , Eric L. Van Nostrand
    • , Alexander A. Shishkin
    • , Wenhao Jin
    • , Nancy L. Allbritton
    •  & Gene W. Yeo

Amendments & Corrections