Volume 13 Issue 2, February 2016

Editorial

Improving databases for human variation

To determine the true pathogenicity of genetic variants, data sharing is essential.

Editorial 28 Jan 2016

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This Month

Jason W. Chin

How to combine biology, chemistry and synthetic biology to add synthetic amino acids to a protein, and why creativity matters.

Vivien Marx
This Month 28 Jan 2016

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Correspondence

Solutions for quantifying P-value uncertainty and replication power

Laura C Lazzeroni
Ying Lu
Ilana Belitskaya-Lévy
Correspondence 28 Jan 2016
Response to Lazzeroni et al.

Lewis G Halsey
Douglas Curran-Everett
Gordon B Drummond
Correspondence 28 Jan 2016
Estimation statistics should replace significance testing

Adam Claridge-Chang
Pryseley N Assam
Correspondence 28 Jan 2016
The mutation significance cutoff: gene-level thresholds for variant predictions

Yuval Itan
Lei Shang
Jean-Laurent Casanova
Correspondence 28 Jan 2016

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Research Highlights

Driving out malaria

Laboratory tests show that gene drives could obliterate wild mosquito populations carrying the malaria parasite, or make them resistant to its transmission.

Tal Nawy
Research Highlights 28 Jan 2016
Simple tools for difficult imaging

Small chemical tweaks during tissue preparation make a big difference for imaging thick, complex biological samples.

Natalie de Souza
Research Highlights 28 Jan 2016
Watching proteins fold on the ribosome

Researchers monitor cotranslational protein folding in real time.

Rita Strack
Research Highlights 28 Jan 2016
Taking a stab at neuronal heterogeneity

Patch-seq integrates functional and molecular properties of single neurons to tackle heterogeneity in the brain.

Nina Vogt
Research Highlights 28 Jan 2016

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Methods in Brief

Counting stoichiometry with super-resolution image data

Methods in Brief 28 Jan 2016
Mouse chimeras for testing human stem cell pluripotency

Methods in Brief 28 Jan 2016
Comparing cancer genome analyses

Methods in Brief 28 Jan 2016
Capturing lysine PTM–dependent interactions

Methods in Brief 28 Jan 2016

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Tools in Brief

Optogenetic mutagenesis in worms

Tools in Brief 28 Jan 2016
Nanobody tools

Tools in Brief 28 Jan 2016
A DIVERSE strategy for discovering peptide tags

Tools in Brief 28 Jan 2016
Efficient unnatural amino acid incorporation

Tools in Brief 28 Jan 2016

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Research Highlights

The condition-dependent proteome

Absolute levels of the Escherichia coli proteome measured under 22 different conditions represent a valuable resource.

Allison Doerr
Research Highlights 28 Jan 2016

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Technology Feature

Genetics: profiling DNA methylation and beyond

Both tried-and-true and new assays are helping labs to assess methylation at particular loci and from single cells.

Vivien Marx

Collection:

NatureTech
Technology Feature 28 Jan 2016

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News & Views

Illuminating translation with ribosome profiling spectra

Software based on the spectral analysis of ribosome profiling improves the detection of translated segments in RNA molecules.

Pavel V Baranov
Audrey M Michel
News & Views 28 Jan 2016
Hidden in the mist no more: physical force in cell biology

To drive its migration through a fibrillar matrix—and thus to spread, invade or metastasize—a cancer cell must exert physical forces. The first visualization of these forces in three dimensions reveals surprising migration dynamics.

Karin Wang
Li-Heng Cai
Jeffrey J Fredberg
News & Views 28 Jan 2016

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Review Article

Editing the epigenome: technologies for programmable transcription and epigenetic modulation

Pratiksha I Thakore
Joshua B Black
Charles A Gersbach

Collection:

The Kavli Prize 2018
Review Article 28 Jan 2016

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Brief Communication

Inverted light-sheet microscope for imaging mouse pre-implantation development

An inverted light-sheet microscope enables imaging of mouse embryos from zygote to blastocyst with minimal photodamage and high resolution for automatic lineage tree reconstruction, allowing new insight into cell fate specification.

Petr Strnad
Stefan Gunther
Jan Ellenberg
Brief Communication 14 Dec 2015
Nanoscale optomechanical actuators for controlling mechanotransduction in living cells

Optomechanical actuator nanoparticles collapse upon illumination with near-infrared light. Appropriately coated, they can be used to mechanically trigger cellular processes such as focal adhesion formation or T cell activation.

Zheng Liu
Yang Liu
Khalid Salaita

Series:

Series on Mechanobiology
Brief Communication 14 Dec 2015
TRP channel mediated neuronal activation and ablation in freely behaving zebrafish

Heterologous TRP channels can be used to stimulate or ablate neurons in response to their chemical or thermal agonists in zebrafish larvae, providing a set of tools orthogonal to optogenetic manipulation.

Shijia Chen
Cindy N Chiu
David A Prober
Brief Communication 14 Dec 2015

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Article

Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro

The small airway-on-a-chip allows the recapitulation of human lung pathophysiology in vitro and analysis of responses to drugs.

Kambez H Benam
Remi Villenave
Donald E Ingber
Article 21 Dec 2015
Genetic code expansion in stable cell lines enables encoded chromatin modification

Stable integration of genes that facilitate the incorporation of unnatural amino acids into the amber stop codon in genes of interest allows targeted integration of acetyl-lysine into histone H3.3 and the investigation of its effects in mouse embryonic stem cells.

Simon J Elsässer
Russell J Ernst
Jason W Chin
Article 04 Jan 2016
Detecting actively translated open reading frames in ribosome profiling data

RiboTaper quantifies the three-nucleotide periodicity in Ribo-seq data to find translated open reading frames (ORFs). The de novo inferred set of ORFs comprehensively defines the cellular proteome across a wide expression range and comprises few additional translated noncoding regions.

Lorenzo Calviello
Neelanjan Mukherjee
Uwe Ohler
Article 14 Dec 2015
Three-dimensional force microscopy of cells in biopolymer networks

Measuring the forces generated by cells is not trivial in materials that behave in a nonlinear fashion. An equation that captures this behavior and finite-element modeling can be used to derive these forces from the material deformations around cells.

Julian Steinwachs
Claus Metzner
Ben Fabry

Series:

Series on Mechanobiology
Article 07 Dec 2015
Engineering an allosteric transcription factor to respond to new ligands

The combination of computational protein design and single-site saturation mutagenesis enables engineering of allosteric transcription factors to respond to new small molecules.

Noah D Taylor
Alexander S Garruss
Srivatsan Raman

Collection:

Nobel Prize in Chemistry 2018
Article 21 Dec 2015

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Advertising Feature: Application Note

Diagenode^® Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage

Anne-Clémence Veillard
Paul Datlinger
Christoph Bock
Advertising Feature: Application Note 28 Jan 2016

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Volume 13 Issue 2, February 2016

Editorial

This Month

Correspondence

Research Highlights

Methods in Brief

Tools in Brief

Research Highlights

Technology Feature

News & Views

Review Article

Brief Communication

Article

Advertising Feature: Application Note

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