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Enhanced CLIP yields complex libraries of RNA components of ribonucleoprotein complexes and maintains single-nucleotide resolution of binding sites. eCLIP enables large scale profiling, as demonstrated with the binding profiles of 73 RBPs in two human cancer cell lines.
Ligand-triggered ribosomal frameshifting allows control of the relative stoichiometry of two proteins and enables the building of logic gates from a single mRNA.
A saposin protein–lipid nanoparticle system stabilizes diverse, fragile membrane proteins in a lipid environment for structural and functional studies.
Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos.
This resource contains 394 human cell type– and tissue-specific transcriptional networks and finds that disease-associated genetic variants often perturb regulatory modules in tissues specific for that disease.
The SWAp-Tag method allows for easy replacement of a tag in a parental strain with an expression cassette of choice to rapidly create new yeast libraries that will enable researchers to address biological questions in Saccharomyces cerevisiae.
HomoFRET sensors based on G6PD homodimerization allow direct sensing of NADPH/NADP+ redox state in living cells. Spectrally tuning the Apollo-NADP+ sensor allows multiplex imaging for studies of oxidative stress in beta cells.
Linking attacks can identify individuals on the basis of seemingly independent data, such as molecular phenotypes and genotypes, in different databases and are a threat to privacy. The authors statistically quantify the extent of this risk and propose means to reduce it.
Spectrally resolved FLIM with three excitation wavelengths and detection on 32 channels combined with advanced pattern matching allows for simultaneous detection and discrimination of fluorophores with nearly identical emission spectra, enabling highly multiplexed imaging.
PLAYR (proximity ligation assay for RNA) enables highly multiplexed transcript quantification in combination with protein marker detection in single cells using flow or mass cytometry.
A near-infrared light–activated genetically encoded photosensitizer composed of a fluorogen-activating protein and a heavy-atom–substituted fluorogen allows protein inactivation and cellular photoablation with high spatiotemporal precision and low collateral damage.
Stable integration of genes that facilitate the incorporation of unnatural amino acids into the amber stop codon in genes of interest allows targeted integration of acetyl-lysine into histone H3.3 and the investigation of its effects in mouse embryonic stem cells.