Single-molecule super-resolution techniques emerged only several years ago but have revolutionized fluorescence microscopy of cellular structures. We discuss some key principles of these techniques, point out pitfalls, highlight recent developments and identify opportunities for the future.
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Acknowledgements
This work was supported by the Systems Biology Initiative (FORSYS) of the Bundesministerium für Bildung und Forschung and by the German Science Foundation (EXC 115).
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Endesfelder, U., Heilemann, M. Art and artifacts in single-molecule localization microscopy: beyond attractive images. Nat Methods 11, 235–238 (2014). https://doi.org/10.1038/nmeth.2852
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DOI: https://doi.org/10.1038/nmeth.2852
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