Method of the Year 2015

Content

• Method of the Year 2015

  The end of 'blob-ology': single-particle cryo-electron microscopy (cryo-EM) is now being used to solve macromolecular structures at high resolution.

  Editorial30 Dec 2015 Nature Methods
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  The field that came in from the cold

  Recent advances in cryo-electron microscopy are enabling researchers to solve protein structures at near-atomic resolutions, expanding the biological applicability of this technique. Michael Eisenstein reports.

  • Michael Eisenstein

  News Feature30 Dec 2015 Nature Methods
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  Single-particle cryo-electron microscopy

  A brief overview of how to solve a macromolecular structure using single-particle cryo-electron microscopy (cryo-EM).

  • Allison Doerr

  Primer30 Dec 2015 Nature Methods
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  The development of cryo-EM into a mainstream structural biology technique

  Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying the structure of challenging systems. The author of this Commentary discusses some of the major historical landmarks in cryo-EM that have led to its present success.

  • Eva Nogales

  Historical Commentary30 Dec 2015 Nature Methods
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  How good can cryo-EM become?

  Cryo-EM has emerged rapidly as a method for determining high-resolution structures of biological macromolecules. The author of this Commentary discusses just how much better this technology may get and how fast such developments are likely to happen.

  • Robert M Glaeser

  Commentary30 Dec 2015 Nature Methods
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  Protein labeling in cells

  Better protein-labeling strategies will improve imaging.

  • Rita Strack

  Method to Watch30 Dec 2015 Nature Methods
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  Unraveling nuclear architecture

  New approaches are needed to see the dynamics of 3D chromatin structure at high resolution and throughput.

  • Nicole Rusk

  Method to Watch30 Dec 2015 Nature Methods
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  Protein structure through time

  Advances in time-resolved crystallography make it possible to follow ever more rapid protein structural changes.

  • Allison Doerr

  Method to Watch30 Dec 2015 Nature Methods
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  Precision optogenetics

  Optogenetic manipulation of neurons at cellular resolution holds promise for the dissection of neural microcircuitry.

  • Nina Vogt

  Method to Watch30 Dec 2015 Nature Methods
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  Highly multiplexed imaging

  Methods for imaging multiple targets in a single cell are breaking the color barrier.

  • Rita Strack

  Method to Watch30 Dec 2015 Nature Methods
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  Deep learning

  New computational tools learn complex motifs from large sequence data sets.

  • Nicole Rusk

  Method to Watch30 Dec 2015 Nature Methods
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  Subcellular maps

  Methods to systematically map the distribution of proteins in cells are evolving.

  • Natalie de Souza

  Method to Watch30 Dec 2015 Nature Methods
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  Integrated single-cell profiles

  Integrated molecular profiles of single cells will provide mechanistic insights into gene regulation and heterogeneity.

  • Tal Nawy

  Method to Watch30 Dec 2015 Nature Methods