Research Highlights in 2018

A new development in high-speed atomic force microscopy enables microsecond resolution.

A vesicle-based mass spectrometry method enables the analysis of protein complexes in native membranes.

Synthetic small molecules can form liquid-phase condensates and simultaneously sequester intracellular enzymes.

A machine learning model predicts the genotype of CRISPR–Cas9 gene editing products, thereby enabling precise, template-free correction of disease-associated mutations.

A macroscope mounted on the head of a freely moving transgenic rat can help shine light on complex behaviors.

Flipping opsins within the membrane diversifies the array of available optogenetics tools.

A new computational method integrates RNA single-cell sequencing and spatial data.

Adaptive light-sheet microscopy enables imaging of mouse development through early organogenesis with single-cell resolution.

Acquisition of RNA into CRISPR arrays allows the recording of transcriptional dynamics in cells.

The concept of massively parallel single-molecule protein sequencing emerges.

Dip-C, a descendent of the 3C method, reveals 3D genome structures of single diploid human cells.

Deep learning pushes the limits of accuracy in genomic variant calling.

Researchers have carried out the first de novo design of a β-barrel protein and engineered the interior to bind and activate a fluorogenic dye.

The European XFEL produces its first protein structures.

Phage biology yields useful anti-CRISPR proteins that may also lead to new CRISPR systems.

FluoEM aligns axon reconstructions from fluorescence and 3D electron microscopy on the same tissue to allow multicolor labeling of distant inputs.

CRISPR–Cas9 enables successive fusions of 16 native chromosomes into one artificial single chromosome.

Studies of tumor immunology will gain from coculture of tumor organoids and peripheral blood cells.

By coupling multiplex iterative indirect immunofluorescence imaging with computer vision methods, researchers can detect at least 40 different proteins with subcellular resolution.

By incorporating an image-classification task into an online video game, the Human Protein Atlas project unlocks the gaming community as a citizen science force.