News & Views in 2022

Multiplexing real-time single virus tracking with imaging paves the way for detailed information on virus–host interactions, offering a potential paradigm shift.

The generation of a whole larval zebrafish brain electron microscopy volume in tandem with automated tools lays the groundwork for producing the first vertebrate brain connectome.

OCTOPUS is a bioengineered device for high-fidelity organoid cultures long term.

An approach for integrating the wealth of heterogeneous brain data — from gene expression and neurotransmitter receptor density to structure and function — allows neuroscientists to easily place their data within the broader neuroscientific context.

TracX shows that fast proofreading of track assignments leads to improved cell tracking performance.

Advances in microscopy, computer vision and open source software are converging to usher in a new era of microscopes that control themselves.

New computational method uses convolutional neural networks for cis-regulatory sequence analysis to analyze and cluster scATAC-seq data.

Dipole–dipole crosstalk between fluorophores separated by a distance of less than 10 nm induces changes in their photophysics, which adds a challenge to localization microscopy in the sub-10-nm regime.

A diagnostic fragment ion in tandem mass spectrometry enables confident protein lactylation assignment and the discovery of broad lysine modification beyond histones.

Evidence for at least one protein product from 80% of all mouse genes is reported in a comprehensive proteomic analysis of 41 adult mouse tissues. Comparison of tissue profiles between mouse and human suggests that the fundamental biology of this important model organism is even more different from our own than we thought.

New data collection methods enable ab initio protein structure determination by micro-electron diffraction for the first time.

De novo structure generation from tandem mass spectrometry data will advance annotation of unknown metabolomic signals.

A repeat-aware pipeline corrects structural assembly errors and polishes assembly sequences in large repeats without overcorrection.

A novel bright near-infrared fluorescent protein inserted into a nanobody enables visualization of native proteins inside living cells and specific manipulation of cell function, including Boolean protein-based operators.

DiMeLo-seq leverages immunotethered DNA methyltransferases with long-read sequencing to map the locations of chromatin proteins in their natural context.

A novel approach to probabilistically align adjacent multiple tissue slices from spatially resolved transcriptomics data provides unprecedented depth for the investigation of tissue architecture and paves the way for new developments in 3D spatial analytics.

A flexible open-top light-sheet microscope has been developed that can perform deep three-dimensional imaging on all clearing protocols with low and high optical resolution.

Two new toolkits that leverage deep-learning approaches can track the positions of multiple animals and estimate poses in different experimental paradigms.

Engineered viral entry combined with single-cell sequencing technology makes it possible to identify specific ligand–receptor interactions in a high-throughput manner.

Global profiling of changes in the reactivity of cysteine residues in response to phosphorylation during mitosis identifies cysteine residues as potential regulatory and drug binding sites on proteins.