Research Highlights in 2021

A yeast cell culture approach mimics the benefits of somatic hypermutation to accelerate the discovery of desirable antibodies.

Development of methods for single-cell proteomics increases depth and throughput

Researchers design DNA shells to trap virus particles and thus prevent interactions between viruses and host cells.

Chromatin accessibility, proteins levels and other features measured from the same cells.

BioID using organelle-specific bait proteins reveals the subcellular HEK293 proteome.

Lineage tracing using somatic mutations as barcodes helps decode human hematopoiesis.

GPCR-based opsins can be harnessed to inhibit neurotransmission via inhibitory G protein signaling.

Advances in adaptive optics combined with a twist on light field microscopy enable high-quality imaging.

Nanopore sequencing enables quantitative profiling of pseudouridine and 2′-O-methylation modifications in native RNAs.

Human cervical organoids mimic dynamics of cancer and sexually transmitted infections.

The iNMF algorithm efficiently integrates large-scale, continually arriving single-cell multi-omic datasets.

An approach involving barcoded viral tracing provides insights into cell–cell interactions at single-cell resolution.

Researchers work towards improving the efficiency and specificity of prime editors, as well as their delivery system in plants and animals.

Composite in situ imaging leverages gene expression patterns to improve the efficiency of highly multiplexed single-molecule FISH measurements by orders of magnitude.

The Cell-TALKING technique probes DNA modifications around a histone modification in fixed cells.

Phased genomes from diverse human populations are assembled using multiple sequencing technologies.

Photonic resonator interferometric scattering microscopy offers label-free imaging of biomolecules in vitro with improved signal-to-noise ratio.

Fusions of human and chimp iPS cells help study how divergence in gene expression affects phenotypic traits.

Two independent teams have developed the first in vitro human blastocysts.

The split-pool barcoding strategy has been extended to single-cell RNA sequencing (scRNA-seq) of microbes.