Live samples are intrinsically highly dynamic, yet techniques to monitor these complex environments usually reflect snapshots, thus making time-lapse imaging necessary to explore temporal progression of biological functions. Recent results indicate that exploiting some basic features of fluorescent protein maturation, such as green-to-red maturation of engineered proteins, should allow probing of temporally regulated information.
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The authors thank H. Mizuno and T. Kogure for preparation of figures and D. Mou for critical reading of the manuscript.
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Miyawaki, A., Karasawa, S. Memorizing spatiotemporal patterns. Nat Chem Biol 3, 598–601 (2007). https://doi.org/10.1038/nchembio1007-598
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DOI: https://doi.org/10.1038/nchembio1007-598
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