A new review article details how new structural insight regarding modular polyketide synthases (PKSs) helps us better understand the organization of catalytic events within a PKS module. The plausible models discussed will likely influence future PKS engineering efforts.

The authors show modular functionality of TetR-like proteins in mammalian cells, separating the protein–DNA and the protein–protein interaction. This allows for engineered ON- and OFF-type responses to stimuli, higher order and multi-input logics.

Kim et al. used directed evolution methods to identify a high-fidelity SpCas9 variant, Sniper2L, which exhibits high general activity but maintains high specificity at a large number of target sites.

Live-cell HRMAS NMR spectroscopy and genome-scale metabolic modeling enable high-resolution analysis of dynamic, anaerobic metabolism in Clostridioides difficile, identifying the confluence of carbohydrate and amino acid fermentations for alanine synthesis.

Applying thermal proteome profiling to acute B cell childhood leukemia cell lines combined with deep peptide fractionation and a graph-based clustering algorithm allows inference of functional proteoform groups and their association with drug response.

Live-cell HRMAS NMR spectroscopy and genome-scale metabolic modeling enable high-resolution analysis of dynamic, anaerobic metabolism in Clostridioides difficile, identifying the confluence of carbohydrate and amino acid fermentations for alanine synthesis.

Kim et al. used directed evolution methods to identify a high-fidelity SpCas9 variant, Sniper2L, which exhibits high general activity but maintains high specificity at a large number of target sites.

The authors show modular functionality of TetR-like proteins in mammalian cells, separating the protein–DNA and the protein–protein interaction. This allows for engineered ON- and OFF-type responses to stimuli, higher order and multi-input logics.

A chemically controlled Cas9 switch offers a simple, general approach to temporal control of Cas9 effectors, including transcriptional activators, base editors and a prime editor. Chemically controlled base editors revealed bystander editing kinetics.

Using an integrated metabologenomics approach, the biosynthetic pathway for the pestalamides is revealed and over 200 high-confidence targets are identified for future studies.