Volume 20 Issue 3, March 2015

Rapid Antidepressant, NMDAR antagonist, AP5, promotes protein stability of 14-3-3eta. Hippocampal neurons expressing 14-3-3eta fused to kaede, a fluorescent marker for protein translation and stability. Top: Vehicle treated neurons show decrease in existing 14-3-3 eta protein 60 minutes after kaede photoconversion. Bottom: AP5 treatment stabilizes protein present at time of photoconversion. For more info on this topic, please refer to the article by Workman et al on pages 298-310.