Abstract
Surface modification of three species of PHAs, PHB, P(HB-co-HV) and P(HB-co-HH), were carried out by 40 keV C+ ion implantations at four different doses, 1 × 1012, 1 × 1013, 1 × 1014, and 1 × 1015 ions cm-2. Remarkable changes between the pristine samples and the implanted samples were observed for the FT-IR and XPS spectra. Fibroblast culture was conducted to investigate the surface biocompatibility changes caused by the implantation of C+. From the results of the MTT colorimetric test and the cell morphology observation assessed by SEM, FI and Confocal Laser Scanning Microscopy (CLSM), there were several evidence that the cytocompatibility of the films were improved by ion implantation and the best cytocompatibility was achieved at the ion dose of 1 × 1012 for all the three species of PHAs. Possible explanation is given by correlating the cell adhesion figures with the chemical and physical changes in the modified surfaces. Results show that the PHAs modified by ion implanting could be a promising family of biomaterials for tissue engineering.
Similar content being viewed by others
Article PDF
References
J. A. Alistair and A. D. Edwin, Microbiol. Rev., 4, 450 (1990).
F. R. Lang, D. Leonard, and H. J. Mathieu, Macromolecules, 31, 6177 (1998).
G.-Q. Chen, and Y. Deng, K. Zhao, “Biopolyesters-polyhydroxyalkanoates and Their Applications as Biomaterials”, IUPAC World Polymer Congress, 39th International Symposium on Macromolecules, Beijing, Preprints 2002, Part 2, p 974.
X. Yang, K. Zhao, and G.-Q. Chen, Biomaterials, 23, 1391 (2002).
D. Flusch, G. Clarotti, K. E. Gecheler, F. Schue, and W. Gopel, J. Membr. Sci., 73, 163 (1992).
S. Luo, A. N. Netravali, J. Appl. Polym. Sci., 73, 1059 (1999).
H. Dong and T. Bell, Surf. Coat. Technol., 111, 29 (1999).
D. M. Zhang, F. Z. Cui, Z. S. Luo, Y. B. Lin, K. Zhao, and G. Q. Chen, Surf. Coat. Technol., 131, 350 (2000).
J. Zhang, X. Ye, X. Yu, and H. Li, Mater. Sci. Eng., B, 84, 200 (2001).
E. H. Lee, G. R. Rao, M. B. Lewis, and L. K. Mansur, J. Mater. Res., 9, 1043 (1994).
M. Iwaki, Nucl. Instrum. Methods Phys. Res., Sect. B, 175, 368 (2001).
W. Liu, S. Yang, C. Li, and Y. Sun, Thin Solid Films, 323, 158 (1998).
S. Yang, S. Qi, W. Liu, C. Li, and Y. Sun, Wear, 211, 64 (1997).
M. Koto, K. Ohno, S. Yoshikado, K. Yukimura, S. Kurooka, Y. Suzuki, A. Kinomura, A. Chayahara, and Y. Horino, Mater. Chem. Phys., 127, 54 (1998).
Y. Suzuki, M. Kusakabe, M. Iwaki, K. Kusakabe, H. Akiba, and M. Suzuki, Mat. Res. Soc. Symp. Proc., 669, 110 (1989).
Y. Suzuki, M. Kusakabe, M. Iwaki, H. Akiba, and K. Kusakabe, Radiat. Phys. Chem., 553, 39 (1992).
Y. Suzuki, M. Kusakabe, H. Akiba, K. Kusakabe, and M. Iwaki, Nucl. Instrum. Methods Phys. Res., Sect. B, 698, B59/60 (1991).
Y. Suzuki, M. Kusakabe, J.-S. Lee, M. Kaibara, M. Iwaki, and H. Sasabe, Nucl. Instrum. Methods Phys. Res., Sect. B, 142, B65 (1992).
H. Tsuji, H. Satoh, S. Ikeda, N. Ikemoto, Y. Gotoh, and J. Ishikawa, Surf. Coat. Technol., 124, 103 (1998).
H. Tsuji, H. Sato, T. Baba, A. Ikemura, Y. Gotoh, and J. Ishikawa, Nucl. Instrum. Methods Phys. Res., Sect. B, 815–819, B166-167 (2000).
G. Zund, Q. Ye, S. P. Hoerstrup, A. Shoeberlein, A. C. Schmid, J. Grunenfelder, P. Vogt, and M. Turina, Eur. J. Cardio-thorac. Surg., 15, 519 (1999).
G. Ciapetti, E. Cenni, L. Pratelli, and A. Pizzoferrato, Biomaterials, 14, 359 (1993).
P. Boargatti, A. M. Martelli, A. Bellacosa, R. Casto, L. Massari, S. Capitani, and L. M. Neri, Federation of European Biochemical Societies, 477, 27 (2000).
E. H. W. Pap, G. P. C. Drummen, V. J. Winter, T. W. A. Kooij, P. Rijken, K. W. A. Wirtz, J. A. F. Opdenkamp, W. J. Hage, and J. A. Post, Federation of European Biochemical Societies, 453, 278 (1999).
D. S. Synder and P. L. C. Small, J. Immunol. Methods, 257, 35 (2001).
H. Q. Zhang and M. L. Lian, “Cell Biology Experiment Methods and Technologies”, Beijing Normal University Press, Beijing, 1992, p 29.
R. U. A. Khan and S. R. P. Silva, Diamond Relat. Mater., 10, 224 (2001).
B. Pignataro, E. Conte, A. Scandurra, and G. Marletta, Biomaterials, 18, 1461 (1997).
C. Satriano, E. Conte, and G. Marletta, Langmuir, 17, 2243 (2001).
C. J. Sofield, S. Sugden, and J. Ing, Vacuum, 44(3/4), 285 (1993).
D. Tsoukalas, Solid-State Electron., 33, 639 (1990).
A. Mas, H. Jaaba, and F. Schue, J. Macromol. Sci., Pure Appl. Chem., A, 34(1), 67 (1997).
D. Briggs, “Surface Analysis of Polymers by XPS and Static SIMS”, Cambridge University Press, New York, N.Y., 1998, p 50.
J. Zhang, Q. Wu, X. Yu, P. Zha, and H. Li, Phys. Status Solidi A, 186, 47 (2001).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, X., Zhang, X., Zhu, Y. et al. Surface Modification of Polyhydroxyalkanoates by Ion Implantation. Characterization and Cytocompatibility Improvement. Polym J 35, 148–154 (2003). https://doi.org/10.1295/polymj.35.148
Issue Date:
DOI: https://doi.org/10.1295/polymj.35.148