Browse Articles

  • Article
    | Open Access

    Using hyperpolarized 13C magnetic resonance imaging (MRI), Timm et al. show that a change in oxidative mitochondrial carbohydrate metabolism precedes cardiac decline in a rat model of heart failure induced by doxorubicin (DOX), a widely used chemotherapeutic agent. This study suggests the possibility of using hyperpolarized MRI to test cancer patients receiving DOX for early signs of cardiotoxicity.

    • Kerstin N. Timm
    • , Charith Perera
    • , Vicky Ball
    • , John A. Henry
    • , Jack J. Miller
    • , Matthew Kerr
    • , James A. West
    • , Eshita Sharma
    • , John Broxholme
    • , Angela Logan
    • , Dragana Savic
    • , Michael S. Dodd
    • , Julian L. Griffin
    • , Michael P. Murphy
    • , Lisa C. Heather
    •  & Damian J. Tyler
  • Article
    | Open Access

    Neville et al. investigate how Streptococcus pneumoniae mitigates metal ion stress. Despite cadmium induced dysregulation of central carbon metabolism and lipid membrane homeostasis, they find that S. pneumoniae can remain viable by selectively utilizing predominately metal-independent cellular pathways. This study provides insights into how bacteria overcome metal ion toxicity.

    • Stephanie L. Neville
    • , Bart A. Eijkelkamp
    • , Amber Lothian
    • , James C. Paton
    • , Blaine R. Roberts
    • , Jason W. Rosch
    •  & Christopher A. McDevitt
  • Article
    | Open Access

    Holman et al. develop a grid-less autonomous adaptive sampling method to explore high-dimensional spatiochemical experimental systems. Their method greatly decreases image acquisition time while improving spatial resolution, and when implemented with FTIR, it outperforms existing standard grid sampling approaches. They further show its utility for a complex biological sample, C. elegans.

    • Elizabeth A. Holman
    • , Yuan-Sheng Fang
    • , Liang Chen
    • , Michael DeWeese
    • , Hoi-Ying N. Holman
    •  & Paul W. Sternberg
  • Article
    | Open Access

    Borer et al. use experimental data and mathematical simulations to show how the expansion behavior of the bacteria P. stutzeri varies when exhibiting different types of trophic interactions. This study offers a unifying explanation for the emergence of patterns attributed to variation in localized growth rates of cells mediated by environmental feedbacks.

    • Benedict Borer
    • , Davide Ciccarese
    • , David Johnson
    •  & Dani Or
  • Article
    | Open Access

    Ishida et al. investigated genome-wide associations between 1068 healthy Japanese adults and their gut microbiota. They find that five loci account for a small variation of the human gut microbiota composition and that six genera are heritable. This study suggests that human gut microbiota composition is likely to be affected by multiple genetic loci.

    • Sachiko Ishida
    • , Kumiko Kato
    • , Masami Tanaka
    • , Toshitaka Odamaki
    • , Ryuichi Kubo
    • , Eri Mitsuyama
    • , Jin-zhong Xiao
    • , Rui Yamaguchi
    • , Satoshi Uematsu
    • , Seiya Imoto
    •  & Satoru Miyano
  • Article
    | Open Access

    Chowdhury Abdulla and Richa Aishwarya et al. report that methamphetamine exposure in mice leads to cardiomyopathy via the signaling receptor Sigmar1. They find that methamphetamine inhibits Sigmar1, which alters mitochondrial function, and show that their findings are consistent with observations in hearts from human methamphetamine users.

    • Chowdhury S. Abdullah
    • , Richa Aishwarya
    • , Shafiul Alam
    • , Mahboob Morshed
    • , Naznin Sultana Remex
    • , Sadia Nitu
    • , Gopi K. Kolluru
    • , James Traylor
    • , Sumitra Miriyala
    • , Manikandan Panchatcharam
    • , Brandon Hartman
    • , Judy King
    • , Mohammad Alfrad Nobel Bhuiyan
    • , Sunitha Chandran
    • , Matthew D. Woolard
    • , Xiuping Yu
    • , Nicholas E. Goeders
    • , Paari Dominic
    • , Connie L. Arnold
    • , Karen Stokes
    • , Christopher G. Kevil
    • , A. Wayne Orr
    •  & Md. Shenuarin Bhuiyan
  • Article
    | Open Access

    Frey and colleagues describe a new fossil shark from the Late Devonian of Morocco in which the specialized jaw articulation is unusually well preserved. Biomechanical modelling reveals that the mandible rolled throughout its movement arc, engaging more of its tooth battery in a clutching bite action.

    • Linda Frey
    • , Michael I. Coates
    • , Kristen Tietjen
    • , Martin Rücklin
    •  & Christian Klug
  • Article
    | Open Access

    Kumar et al. use proteomes of patients with falciparum and vivax malaria and dengue fever, and identify differentially abundant proteins and their associated functions in the different severities and diseases. This study offers mechanistic pathways to malaria severity, and adopts a machine learning approach for predictive proteins for the malarial types and severities.

    • Vipin Kumar
    • , Sandipan Ray
    • , Shalini Aggarwal
    • , Deeptarup Biswas
    • , Manali Jadhav
    • , Radha Yadav
    • , Sanjeev V. Sabnis
    • , Soumaditya Banerjee
    • , Arunansu Talukdar
    • , Sanjay K. Kochar
    • , Suvin Shetty
    • , Kunal Sehgal
    • , Swati Patankar
    •  & Sanjeeva Srivastava
  • Article
    | Open Access

    Gao et al. design a lung targeting nanovesicles made from human neutrophils membrane, and co-loaded inflammatory resolving mediator RvD1 and antibiotic CAZ inside the nanovesicles. These nanovesicles could accelerate the inflammation resolution and release the antibiotics to treat bacterial infections in mice. This approach is useful in developing multifunctional nanoparticle-based therapeutics for lung bacterial infections treatment.

    • Jin Gao
    • , Sihan Wang
    • , Xinyue Dong
    • , Leon G. Leanse
    • , Tianhong Dai
    •  & Zhenjia Wang
  • Article
    | Open Access

    Ito et al. succeed in detecting guanosine tetraphosphate (ppGpp) in measurable levels in metazoan, specifically in Drosophila. They further demonstrate that the ppGpp-specific hydrolase, metazoan SpoT homolog-1 (Mesh1), is necessary, at least in certain conditions, to maintain low ppGpp levels, hence providing insights into the role of Mesh1 as a ppGpp hydrolase in vivo.

    • Doshun Ito
    • , Hinata Kawamura
    • , Akira Oikawa
    • , Yuta Ihara
    • , Toshio Shibata
    • , Nobuhiro Nakamura
    • , Tsunaki Asano
    • , Shun-Ichiro Kawabata
    • , Takashi Suzuki
    •  & Shinji Masuda
  • Article
    | Open Access

    Tao Zhu et al. present UMI-ATAC-seq, an improved ATAC-seq technique that uses unique molecular identifiers to avoid filtering out true independent - but identical - Tn5 insertion events that would otherwise be treated as PCR duplicates. They apply UMI-ATAC-seq to rice panicle samples and show that their approach improves the quantification of chromatin accessibility and footprinting.

    • Tao Zhu
    • , Keyan Liao
    • , Rongfang Zhou
    • , Chunjiao Xia
    •  & Weibo Xie
  • Article
    | Open Access

    Miguel et al. identify a new enzyme belonging to the GDSL lipase-like family that is involved in the final stage of transformation of 5-CQA into 3,5-diCQA. This enzyme is able to realize an efficient transformation by over 60%, making the transformation process a valuable technological tool that can be easily transferred on an industrial scale.

    • Sissi Miguel
    • , Guillaume Legrand
    • , Léonor Duriot
    • , Marianne Delporte
    • , Barbara Menin
    • , Cindy Michel
    • , Alexandre Olry
    • , Gabrielle Chataigné
    • , Aleksander Salwinski
    • , Joakim Bygdell
    • , Dominique Vercaigne
    • , Gunnar Wingsle
    • , Jean Louis Hilbert
    • , Frédéric Bourgaud
    • , Alain Hehn
    •  & David Gagneul
  • Article
    | Open Access

    Berryman et al demonstrate that disaggregated cells can be phenotyped with a high degree of accuracy from bright-field and non-specifically stained microscopy images using a trained convolutional neural network (CNN). This approach allows for the identification of cell types without the need for specific markers.

    • Samuel Berryman
    • , Kerryn Matthews
    • , Jeong Hyun Lee
    • , Simon P. Duffy
    •  & Hongshen Ma
  • Article
    | Open Access

    Kwan et al. show the interaction between the HACS1 SH3 domain and a sequence near the third immunoreceptor tyrosine-based inhibition motif of the Paired immunoglobulin receptor B (PIRB). This study suggests that the HACS1-PIRB partnership has the potential to unite signaling pathways that regulate both immune and neuronal cell fate.

    • Jamie J. Kwan
    • , Sladjana Slavkovic
    • , Michael Piazza
    • , Dingyan Wang
    • , Thorsten Dieckmann
    • , Philip E. Johnson
    • , Xiao-Yan Wen
    •  & Logan W. Donaldson
  • Article
    | Open Access

    Rosanna Sestito et al. report a miR-200b/c-mediated regulatory circuit that drives ovarian cancer metastasis via the endothelin A receptor (ETAR). They show that blockade of ETAR increases miR-200b/c expression and subsequently reduces ZEB1 expression, thereby inhibiting further metastatic progression.

    • Rosanna Sestito
    • , Roberta Cianfrocca
    • , Piera Tocci
    • , Laura Rosanò
    • , Andrea Sacconi
    • , Giovanni Blandino
    •  & Anna Bagnato
  • Article
    | Open Access

    Britney Helling et al. report that cultured bronchial cells from adults with asthma show different gene expression and chromatin accessibility patterns when exposed to rhinovirus than do cells from individuals without asthma. Their data suggest that rhinovirus infection leads to a delayed or elongated activation of inflammatory genes in individuals with asthma compared to those without asthma.

    • Britney A. Helling
    • , Débora R. Sobreira
    • , Grace T. Hansen
    • , Noboru J. Sakabe
    • , Kaixuan Luo
    • , Christine Billstrand
    • , Bharathi Laxman
    • , Raluca I. Nicolae
    • , Dan L. Nicolae
    • , Yury A. Bochkov
    • , James E. Gern
    • , Marcelo A. Nobrega
    • , Steven R. White
    •  & Carole Ober
  • Review Article
    | Open Access

    Shuai Jiang discusses the regulation and functional role of the Tet2 enzyme, a regulator of lymphoid and myeloid cell development frequently mutated in haematopoietic cancers. He highlights how Tet2 activity may be modulated to influence cancer immune responses.

    • Shuai Jiang
  • Article
    | Open Access

    Yuan et al. propose an unsupervised deep learning network approach to analyze the dynamics of membrane proteins from the fluorescence intensity traces. The unsupervised nature facilitates training of the system without predefined state number or pre-labelling and can even extract information from noise distribution.

    • Jinghe Yuan
    • , Rong Zhao
    • , Jiachao Xu
    • , Ming Cheng
    • , Zidi Qin
    • , Xiaolong Kou
    •  & Xiaohong Fang
  • Article
    | Open Access

    Albarracín Orio et al. show that an environmental bacterial strain of Bacillus subtilis undergoes heritable phenotypic variation when it interacts with a soil fungal pathogen Setophoma terrestris. This study suggests B. subtilis metabolism may be rewired to increase the production of antifungal compounds during its antagonistic interaction with S. terrestris.

    • Andrea G. Albarracín Orio
    • , Daniel Petras
    • , Romina A. Tobares
    • , Alexander A. Aksenov
    • , Mingxun Wang
    • , Florencia Juncosa
    • , Pamela Sayago
    • , Alejandro J. Moyano
    • , Pieter C. Dorrestein
    •  & Andrea M. Smania
  • Article
    | Open Access

    Ceolin et al. optimise the fluorescent protein mNeonGreen to generate a reporter to measure enhancer expression in living Drosophila embryos. They also find that reduced enhancer–promoter distance or addition of binding sites for the pioneer transcription factor Zelda increases enhancer activity.

    • Stefano Ceolin
    • , Monika Hanf
    • , Marta Bozek
    • , Andrea Ennio Storti
    • , Nicolas Gompel
    • , Ulrich Unnerstall
    • , Christophe Jung
    •  & Ulrike Gaul
  • Article
    | Open Access

    Sylvie Gimenez et al. compare adaptation by copy number variation in two geographically distinct populations of the fall armyworm and find a relationship between copy number variation and insecticide resistance. The authors perform toxicological tests to confirm a role of the CYP gene cluster in insecticide resistance and suggest that evolutionary forces causing insecticide resistance can be independent of host-plant adaptation.

    • Sylvie Gimenez
    • , Heba Abdelgaffar
    • , Gaelle Le Goff
    • , Frédérique Hilliou
    • , Carlos A. Blanco
    • , Sabine Hänniger
    • , Anthony Bretaudeau
    • , Fabrice Legeai
    • , Nicolas Nègre
    • , Juan Luis Jurat-Fuentes
    • , Emmanuelle d’Alençon
    •  & Kiwoong Nam
  • Article
    | Open Access

    Lan et al. make single and triple phosphomimetic mutations in a long flexible loop domain of BCL-2 and show that these mutations reduce structural flexibility and promote ligand access to the groove. Their study indicates that changes in structural flexibility on an unstructured loop can activate an allosteric, otherwise inactive protein.

    • Yu-Jing Lan
    • , Pei-Shan Yeh
    • , Te-Yu Kao
    • , Yuan-Chao Lo
    • , Shih-Che Sue
    • , Yu-Wen Chen
    • , Dennis W. Hwang
    •  & Yun-Wei Chiang
  • Article
    | Open Access

    Sato et al. find that WNK (With No Lysine [K]) acts as a positive regulator of the Wnt signaling pathway by attenuating the interaction between β-Catenin and the Glucose Induce degradation Deficient (GID) complex, and show that a WNK inhibitor also functions as a Wnt inhibitor, suppressing xenograft tumor development in mice. These findings suggest that WNK is a regulator of β-Catenin and a potential therapeutic target

    • Atsushi Sato
    • , Masahiro Shimizu
    • , Toshiyasu Goto
    • , Hiroyuki Masuno
    • , Hiroyuki Kagechika
    • , Nobuyuki Tanaka
    •  & Hiroshi Shibuya
  • Article
    | Open Access

    Rosendo-Pineda et al. discover that N-methyl-D-aspartate receptors (NMDAR) mediate transient calcium entry in mitosis, dependent on phosphorylation of NMDAR by Cyclin B1/CDK1. They also find that phosphorylation-mimicking mutants of NMDAR cause mitotic defects, suggesting that precise control of NMDAR-mediated calcium entry is needed for proper mitosis.

    • Margarita Jacaranda Rosendo-Pineda
    • , Juan Jesus Vicente
    • , Oscar Vivas
    • , Jonathan Pacheco
    • , Arlet Loza-Huerta
    • , Alicia Sampieri
    • , Linda Wordeman
    • , Claudia Moreno
    •  & Luis Vaca
  • Article
    | Open Access

    Huang et al. use proteomics to examine liver and liver mitochondria of hibernating bats. The authors observe the unfolded protein response in endoplasmic reticulum and co-activation of survival signaling pathways in torpid bats and provide further insight into physiological adaptations that allow bats to survive a long period of hibernation.

    • Wenjie Huang
    • , Chen-Chung Liao
    • , Yijie Han
    • , Junyan Lv
    • , Ming Lei
    • , Yangyang Li
    • , Qingyun Lv
    • , Dong Dong
    • , Shuyi Zhang
    • , Yi-Husan Pan
    •  & Jian Luo
  • Article
    | Open Access

    By performing an ecologically relevant experiment with variety in litter composition and detritivore species, Joly et al. find that litter conversion into faeces has a profound effect on organic matter lability and decomposition. These findings have implications for biogeochemical models of carbon cycling.

    • François-Xavier Joly
    • , Sylvain Coq
    • , Mathieu Coulis
    • , Jean-François David
    • , Stephan Hättenschwiler
    • , Carsten W. Mueller
    • , Isabel Prater
    •  & Jens-Arne Subke
  • Article
    | Open Access

    By combining whole genome sequencing and metabolomics, Koshiba et al. identify a large number of associations between genetic variants and plasma metabolites. They find that some genetic variants affect the interaction between the host and symbiotic bacteria. This study provides insights into how genetic and environmental factors interact to shape human metabolism.

    • Seizo Koshiba
    • , Ikuko N. Motoike
    • , Daisuke Saigusa
    • , Jin Inoue
    • , Yuichi Aoki
    • , Shu Tadaka
    • , Matsuyuki Shirota
    • , Fumiki Katsuoka
    • , Gen Tamiya
    • , Naoko Minegishi
    • , Nobuo Fuse
    • , Kengo Kinoshita
    •  & Masayuki Yamamoto
  • Article
    | Open Access

    Stephen Doyle et al. report the chromosome-scale genome assembly and transcriptome sequence data of Haemonchus contortus, a key parasitic nematode model. These data show nearly complete conservation of chromosome content with C. elegans and brings insight into transcriptional regulation and chromosome-wide genetic diversity in this important pathogen.

    • Stephen R. Doyle
    • , Alan Tracey
    • , Roz Laing
    • , Nancy Holroyd
    • , David Bartley
    • , Wojtek Bazant
    • , Helen Beasley
    • , Robin Beech
    • , Collette Britton
    • , Karen Brooks
    • , Umer Chaudhry
    • , Kirsty Maitland
    • , Axel Martinelli
    • , Jennifer D. Noonan
    • , Michael Paulini
    • , Michael A. Quail
    • , Elizabeth Redman
    • , Faye H. Rodgers
    • , Guillaume Sallé
    • , Muhammad Zubair Shabbir
    • , Geetha Sankaranarayanan
    • , Janneke Wit
    • , Kevin L. Howe
    • , Neil Sargison
    • , Eileen Devaney
    • , Matthew Berriman
    • , John S. Gilleard
    •  & James A. Cotton
  • Article
    | Open Access

    Branconi et al. report on an experimental approach to investigate the ecological and social drivers of cooperative breeding in clownfish. Experiments were performed on a wild population in Papua New Guinea and indicate that clownfish non-breeding strategies can be understood using economic bargaining theory and cooperative breeding theory.

    • Rebecca Branconi
    • , Tina A. Barbasch
    • , Robin K. Francis
    • , Maya Srinivasan
    • , Geoffrey P. Jones
    •  & Peter M. Buston
  • Article
    | Open Access

    Maria Teschler-Nicola et al. use ancient DNA sequencing to report the earliest known case of human monozygotic twins found in a previously discovered Upper Palaeolithic burial site. Using bioanthropological and archaeological techniques, they also find that the twins were full-term newborns and that ancient mortuary behavior included re-opening of grave sites to bury related individuals together.

    • Maria Teschler-Nicola
    • , Daniel Fernandes
    • , Marc Händel
    • , Thomas Einwögerer
    • , Ulrich Simon
    • , Christine Neugebauer-Maresch
    • , Stefan Tangl
    • , Patrick Heimel
    • , Toni Dobsak
    • , Anika Retzmann
    • , Thomas Prohaska
    • , Johanna Irrgeher
    • , Douglas J. Kennett
    • , Iñigo Olalde
    • , David Reich
    •  & Ron Pinhasi
  • Article
    | Open Access

    Osakabe et al. report the implementation of a CRISPR type I-D (TiD) system for editing in plants, which consists of eight Cas genes (Cas1d–Cas7d, Cas10d) followed by an array of repeat spacer units. The CRISPR TiD can effectively induce short indels, long-range deletions and bi-allelic mutations in plants, adding a valuable toolbox to manipulate plant genomes.

    • Keishi Osakabe
    • , Naoki Wada
    • , Tomoko Miyaji
    • , Emi Murakami
    • , Kazuya Marui
    • , Risa Ueta
    • , Ryosuke Hashimoto
    • , Chihiro Abe-Hara
    • , Bihe Kong
    • , Kentaro Yano
    •  & Yuriko Osakabe
  • Article
    | Open Access

    Shark cells are difficult to culture in the laboratory as the high osmolality physiological conditions do not reflect those of commonly cultured mammalian or teleost fish cell lines. Uno et al. present a new method of culturing shark fibroblasts and lymphocytes, and characterized the karyotypes of four carpet shark species, enabling whole genome analysis of these species.

    • Yoshinobu Uno
    • , Ryo Nozu
    • , Itsuki Kiyatake
    • , Nobuyuki Higashiguchi
    • , Shuji Sodeyama
    • , Kiyomi Murakumo
    • , Keiichi Sato
    •  & Shigehiro Kuraku
  • Article
    | Open Access

    Hyo-Jun Lee et al. show that submergence in Arabidopsis deactivates wound-induced defence against herbivore attack by suppressing the expression of jasmonic acid biosynthesis genes and increasing expression of ethylene-responsive genes. These results shed light on how flooding may impact plant defence systems.

    • Hyo-Jun Lee
    • , Ji-Sun Park
    • , Seung Yong Shin
    • , Sang-Gyu Kim
    • , Gisuk Lee
    • , Hyun-Soon Kim
    • , Jae Heung Jeon
    •  & Hye Sun Cho
  • Article
    | Open Access

    Stuhr and Curran profile the metabolic, lifespan and health outcomes of C. elegans under six bacterial diets: the three most commonly used E. coli strains OP50, HT115, HB101, along with Methylobacterium, Xanthomonas, and Sphingomonas. These results show how diet can differentially alter multiple life history traits including development, reproduction, and metabolism, and can provide a better understanding of the complexities of bacterial diets and subsequent changes in physiology and gene expression.

    • Nicole L. Stuhr
    •  & Sean P. Curran
  • Article
    | Open Access

    The Doushantuo biota of China is incredibly important for understanding the evolution of complex animals. Willman et al. report on evidence of Doushantuo-like biota from the Ediacaran of North Greenland, indicating that these important organisms were distributed across multiple palaeocontinents.

    • Sebastian Willman
    • , John S. Peel
    • , Jon R. Ineson
    • , Niels H. Schovsbo
    • , Elias J. Rugen
    •  & Robert Frei
  • Article
    | Open Access

    Strack et al find that Polymerase δ interacting protein 38 (PDIP38) is targeted to the mitochondrial matrix where it colocalises with the mitochondrial AAA + protein CLPXP. PDIP38 modulates the specificity of CLPXP in vitro and alters the stability of CLPX in vitro and in cells. The PDIP38 structure leads the authors to speculate that PDIP38 is a CLPXP adaptor.

    • Philip R. Strack
    • , Erica J. Brodie
    • , Hanmiao Zhan
    • , Verena J. Schuenemann
    • , Liz J. Valente
    • , Tamanna Saiyed
    • , Bradley R. Lowth
    • , Lauren M. Angley
    • , Matthew A. Perugini
    • , Kornelius Zeth
    • , Kaye N. Truscott
    •  & David A. Dougan
  • Article
    | Open Access

    Beatson et al. show that a sialylated tumour-associated glycoform of the mucin MUC1 induces the differentiation of monocytes into tumour-associated macrophages. These macrophages are found in breast cancer stroma and their presence is associated with poor prognosis.

    • Richard Beatson
    • , Rosalind Graham
    • , Fabio Grundland Freile
    • , Domenico Cozzetto
    • , Shichina Kannambath
    • , Ester Pfeifer
    • , Natalie Woodman
    • , Julie Owen
    • , Rosamond Nuamah
    • , Ulla Mandel
    • , Sarah Pinder
    • , Cheryl Gillett
    • , Thomas Noll
    • , Ihssane Bouybayoune
    • , Joyce Taylor-Papadimitriou
    •  & Joy M. Burchell
  • Article
    | Open Access

    Workenhe et al. show in mice that a combination of oncolytic HSV-1 virus and Mitomycin-C activates an inflammatory response, through necroptosis induction, that renders tumours susceptible to immune checkpoint inhibitors. These findings informs on the potential role of necroptosis in immunotherapy approaches.

    • Samuel T. Workenhe
    • , Andrew Nguyen
    • , David Bakhshinyan
    • , Jiarun Wei
    • , David N. Hare
    • , Kelly L. MacNeill
    • , Yonghong Wan
    • , Andrew Oberst
    • , Jonathan L. Bramson
    • , Jalees A. Nasir
    • , Alyssa Vito
    • , Nader El-Sayes
    • , Sheila K. Singh
    • , Andrew G. McArthur
    •  & Karen L. Mossman
  • Research Highlight
    | Open Access

    While much of the work examining the ecological effects of marine warming events focuses on the magnitude and duration of elevated temperatures, a recent study from Amatzia Genin and colleagues investigates how the rate of onset of warming affects the mortality of reef fish in the Red Sea. These authors document fish mortality following two warming events with dramatic increases in temperature and report that piscivores and benthic grazers were disproportionately represented among the found carcasses. Many of these fish were infected with a bacterial pathogen following the warming event. This study points to the rate of warming increase as a critical parameter to be considered when assessing the ecological effects of marine warming events, including those for which the peak temperature is not anomalous.

    • Caitlin Karniski
  • Article
    | Open Access

    The authors report a single cell-resolution gene expression atlas for the developing mouse liver and gallbladder using a transgenic Foxa2eGFP mouse line. By tracing the development of cells from gut endoderm to hepatoblasts they identify key transcriptional changes during liver specification.

    • Tianhao Mu
    • , Liqin Xu
    • , Yu Zhong
    • , Xinyu Liu
    • , Zhikun Zhao
    • , Chaoben Huang
    • , Xiaofeng Lan
    • , Chengchen Lufei
    • , Yi Zhou
    • , Yixun Su
    • , Luang Xu
    • , Miaomiao Jiang
    • , Hongpo Zhou
    • , Xinxin Lin
    • , Liang Wu
    • , Siqi Peng
    • , Shiping Liu
    • , Susanne Brix
    • , Michael Dean
    • , Norris R. Dunn
    • , Kenneth S. Zaret
    • , Xin-Yuan Fu
    •  & Yong Hou
  • Article
    | Open Access

    Gowripalan, Smith et al. use CRISPR/Cas9 technology to rapidly select recombinant poxviruses without using selectable marker genes. They find that Cas9 cleavage inhibits poxvirus DNA replication, suppressing virus spread in culture. This application makes poxviruses more attractive vector platforms for fighting cancer and emerging disease outbreaks.

    • Anjali Gowripalan
    • , Stewart Smith
    • , Tijana Stefanovic
    •  & David C. Tscharke
  • Article
    | Open Access

    Nirav Chhabra et al. characterize adult mice carrying a homozygous mutation in Pax6 that was identified in a patient with foveal hypoplasia. They find that the Pax6 point mutation has pleiotropic effects, including defects in the mouse retinal structures, loss of the optic nerve, changes in energy metabolism and circadian rhythms, and dysregulation of genes expressed in the pancreas.

    • Nirav Florian Chhabra
    • , Oana Veronica Amarie
    • , Moya Wu
    • , Anna-Lena Amend
    • , Marina Rubey
    • , Daniel Gradinger
    • , Martin Irmler
    • , Johannes Beckers
    • , Birgit Rathkolb
    • , Eckhard Wolf
    • , Annette Feuchtinger
    • , Peter Huypens
    • , Raffaele Teperino
    • , Jan Rozman
    • , Gerhard K. H. Przemeck
    •  & Martin Hrabě de Angelis
  • Article
    | Open Access

    Ruhlandt et al. report a plasmonic nanocavity-based method to measure absolute values of quantum yield of commonly used fluorescent proteins. The method is calibration-free, does not require knowledge about maturation or potential dark states, and works on minute amounts of sample. Authors are further able to determine lifetime and quantum yield of several fluorescent proteins, which would be a good resource for researchers working with them.

    • Daja Ruhlandt
    • , Martin Andresen
    • , Nickels Jensen
    • , Ingo Gregor
    • , Stefan Jakobs
    • , Jörg Enderlein
    •  & Alexey I. Chizhik
  • Article
    | Open Access

    Joseph Korpela et al. demonstrate the use of machine-learning assisted bio-loggers on black-tailed gulls and streaked shearwaters. As video recording is only activated through variations in movement detected by low-cost accelerometers, this method represents improvements to runtime and precision over existing bio-logging technology.

    • Joseph Korpela
    • , Hirokazu Suzuki
    • , Sakiko Matsumoto
    • , Yuichi Mizutani
    • , Masaki Samejima
    • , Takuya Maekawa
    • , Junichi Nakai
    •  & Ken Yoda