Browse Articles

  • Article | | open

    Giancarlo Abis et al. show that 15-deoxy-Δ12,14-Prostaglandin J2 inhibits the human soluble epoxide hydrolase by reversibly binding its catalytic pocket or by covalently binding reactive cysteines far from the active site. Inhibitors targeting these allosteric sites might prove to be useful therapeutic strategies.

    • Giancarlo Abis
    • , Rebecca L. Charles
    • , Jolanta Kopec
    • , Wyatt W. Yue
    • , R. Andrew Atkinson
    • , Tam T. T. Bui
    • , Steven Lynham
    • , Simona Popova
    • , Yin-Biao Sun
    • , Franca Fraternali
    • , Philip Eaton
    •  & Maria R. Conte
  • Article | | open

    Natalija Kashuba et al. analysed human DNA from ancient chewing gums collected from western Sweden. They provide genomic information for three Scandinavian hunter-gatherers, expanding the range of this genetic group and linking them to eastern lithic technologies.

    • Natalija Kashuba
    • , Emrah Kırdök
    • , Hege Damlien
    • , Mikael A. Manninen
    • , Bengt Nordqvist
    • , Per Persson
    •  & Anders Götherström
  • Article | | open

    Lukas Weber et al. present diffcyt, an open-source computational framework for differential analysis of high-dimensional flow and mass cytometry data. The diffcyt framework uses high-resolution clustering and empirical Bayes moderated tests to provide improved statistical performance, flexible experimental designs, and fast runtimes.

    • Lukas M. Weber
    • , Malgorzata Nowicka
    • , Charlotte Soneson
    •  & Mark D. Robinson
  • Article | | open

    Sasha Tetu et al. have examined the effects of plastic leachate exposure on the marine bacteria Prochlorococcus, the most abundant photosynthetic organism on Earth. They find plastic leachates impair growth, photosynthetic capacity and cause global changes in transcription.

    • Sasha G. Tetu
    • , Indrani Sarker
    • , Verena Schrameyer
    • , Russell Pickford
    • , Liam D. H. Elbourne
    • , Lisa R. Moore
    •  & Ian T. Paulsen
  • Article | | open

    Yuya Sato et al. present de novo RNA sequencing of microbial communities from two activated sludge bioreactors that differ in their heavy-oil degradation performance. They find that the higher performing bioreactor showed higher expression of nitrification genes, even though nitrifiers represent a small minority of the community.

    • Yuya Sato
    • , Tomoyuki Hori
    • , Hideaki Koike
    • , Ronald R. Navarro
    • , Atsushi Ogata
    •  & Hiroshi Habe
  • Article | | open

    Andrew Chojnacki et al develop reporters to enable real-time in vivo and ex vivo imaging of tissue-resident eosinophils inside several organs in mice. They describe eosinophil behaviours such as active patrolling of the lymph nodes, crawling and clustering.

    • Andrew Chojnacki
    • , Katarzyna Wojcik
    • , Björn Petri
    • , Gurpreet Aulakh
    • , Elizabeth A. Jacobsen
    • , William E. LeSuer
    • , Pina Colarusso
    •  & Kamala D. Patel
  • Article | | open

    Takashi Nagata et al. use UV-visible spectroscopic analysis to show that Ser186 increases the retinylidene Schiff base (SB) pKa of a spider rhodopsin in the inactive state but not after light activation. The change in contribution of Ser186 to the SB pKa suggests that the counterion (Glu181)–SB interaction rearranges upon light activation.

    • Takashi Nagata
    • , Mitsumasa Koyanagi
    • , Hisao Tsukamoto
    • , Eshita Mutt
    • , Gebhard F. X. Schertler
    • , Xavier Deupi
    •  & Akihisa Terakita
  • Article | | open

    Blondelle & Marrocco et al. show that deletion of both Obsl1 and obscurin in skeletal muscle leads to severe alterations in sarcolemma organization, protein expression profiles, and cellular calcium handling. These alterations affected muscle physiology and suggest redundant roles for Obsl1 and obscurin in murine muscle function.

    • Jordan Blondelle
    • , Valeria Marrocco
    • , Madison Clark
    • , Patrick Desmond
    • , Stephanie Myers
    • , Jim Nguyen
    • , Matthew Wright
    • , Shannon Bremner
    • , Enrico Pierantozzi
    • , Samuel Ward
    • , Eric Estève
    • , Vincenzo Sorrentino
    • , Majid Ghassemian
    •  & Stephan Lange
  • Article | | open

    Lu, Tang, Liu et al. present a 3D super-resolution fluorescence microscope that uses selective plane illumination and a spontaneously blinking dye for localization-based imaging. They show that their microscope can be used to study complex biological structures.

    • Chieh-Han Lu
    • , Wei-Chun Tang
    • , Yen-Ting Liu
    • , Shu-Wei Chang
    • , Frances Camille M. Wu
    • , Chin-Yi Chen
    • , Yun-Chi Tsai
    • , Shun-Min Yang
    • , Chiung-Wen Kuo
    • , Yasushi Okada
    • , Yeu-Kuang Hwu
    • , Peilin Chen
    •  & Bi-Chang Chen
  • Article | | open

    Meliha Mehmeti et al. show that Wnt5a is a direct ligand of toll-like receptor 2 and 4 to act as an endogenous tolerance-associated molecular pattern, triggering innate immune tolerance in human myeloid cells. This study expands the functional repertoire of Wnt5a that is mainly known as a signalling protein involved in development and cancer.

    • Meliha Mehmeti
    • , Caroline Bergenfelz
    • , Eva Källberg
    • , Camilla Rydberg Millrud
    • , Per Björk
    • , Fredrik Ivars
    • , Bengt Johansson-Lindbom
    • , Sven Kjellström
    • , Ingemar André
    •  & Karin Leandersson
  • Article | | open

    Diana Carvalho, Kathryn R. Taylor et al. show that ALK2 inhibitors induce apoptosis of ACVR1 mutant diffuse intrinsic pontine glioma cells in vitro. These compounds also increase the survival of mice carrying ACVR1 mutant brainstem xenografts, suggesting ALK2 as a potential pharmacological target against this lethal cancer.

    • Diana Carvalho
    • , Kathryn R. Taylor
    • , Nagore Gene Olaciregui
    • , Valeria Molinari
    • , Matthew Clarke
    • , Alan Mackay
    • , Ruth Ruddle
    • , Alan Henley
    • , Melanie Valenti
    • , Angela Hayes
    • , Alexis De Haven Brandon
    • , Suzanne A. Eccles
    • , Florence Raynaud
    • , Aicha Boudhar
    • , Michelle Monje
    • , Sergey Popov
    • , Andrew S. Moore
    • , Jaume Mora
    • , Ofelia Cruz
    • , Mara Vinci
    • , Paul E. Brennan
    • , Alex N. Bullock
    • , Angel Montero Carcaboso
    •  & Chris Jones
  • Article | | open

    Qi, Li, Yun, Zhang et al.show that lactobacilli promote the generation of gut mucosa by facilitating the conversion of arginine to L-Ornithine. This study highlights the importance of a crosstalk between arginine metabolism in Lactobacilli and tryptophan metabolism in gut epithelial cells in maintaining a healthy gut barrier.

    • Houbao Qi
    • , Yuanyuan Li
    • , Huan Yun
    • , Tong Zhang
    • , Yugang Huang
    • , Jiang Zhou
    • , Hui Yan
    • , Jianmei Wei
    • , Yingquan Liu
    • , Zhiqian Zhang
    • , Yunhuan Gao
    • , Yongzhe Che
    • , Xiaomin Su
    • , Dashuai Zhu
    • , Yuan Zhang
    • , Jin Zhong
    •  & Rongcun Yang
  • Article | | open

    Cecilia Sanchez et al. investigate the organization of immune-variant adhesins on Plasmodium falciparum-infected erythrocytes by super-resolution microscopy. Using a fluorescently tagged protein, they obtain quantitative and spatial information about the distribution of adhesin molecules on knobs, which are structures used by the malaria parasite to anchor to the host cells.

    • Cecilia P. Sanchez
    • , Christos Karathanasis
    • , Rodrigo Sanchez
    • , Marek Cyrklaff
    • , Julia Jäger
    • , Bernd Buchholz
    • , Ulrich S. Schwarz
    • , Mike Heilemann
    •  & Michael Lanzer
  • Article | | open

    Kristīne Grāve et al. report the crystal structure of phosphatidylinositol phosphate synthase from Mycobacterium tuberculosis in its apo-state, in complex with Mn2+ and citrate, and bound to its CDP-DAG substrate. The structures provide atomic details of substrate binding and the dynamics of the catalytic metal site.

    • Kristīne Grāve
    • , Matthew D. Bennett
    •  & Martin Högbom
  • Article | | open

    Ranhja et al. find that budding yeast Dna2 is modified by sumoylation, and that sumoylation reduces the nuclease, but not helicase activity of Dna2 in vitro. In cells, expression of a Dna2 version that cannot be sumoylated leads to impaired DNA end resection and cell division.

    • Lepakshi Ranjha
    • , Maryna Levikova
    • , Veronika Altmannova
    • , Lumir Krejci
    •  & Petr Cejka
  • Article | | open

    Juan Feng et al. show that hawk tea extract reduces cholesterol by suppressing Niemann-Pick C1 like 1-mediated free cholesterol uptake and decreasing the production of very low-density lipoprotein through hepatocyte nuclear factor 4 alpha. This study suggests a therapeutic potential of hawk tea extract for hypercholesterolemia.

    • Juan Feng
    • , Jian Yang
    • , Yujun Chang
    • , Liansheng Qiao
    • , Honglei Dang
    • , Kun Luo
    • , Hongyan Guo
    • , Yannan An
    • , Chengmei Ma
    • , Hong Shao
    • , Jie Tian
    • , Yuan Yuan
    • , Lan Xie
    • , Wanli Xing
    •  & Jing Cheng
  • Article | | open

    Mukherjee, Karnam, Das et al. show that Wuchereria bancrofti microfilarial sheath antigen induces maturation of human dendritic cells, driving Th1 and regulatory T cell responses. This study illustrates how the most prevalent microfilaria W. bancrofti exploits the host innate immunity to cause the immunopathogenesis of lymphatic filariasis.

    • Suprabhat Mukherjee
    • , Anupama Karnam
    • , Mrinmoy Das
    • , Santi P. Sinha Babu
    •  & Jagadeesh Bayry
  • Article | | open

    Karina Nakayama et al. bioengineer endothelialized mouse skeletal muscle using parallel-aligned nanofibrillar scaffolds. They find that muscle produced using the aligned scaffolds developed a spatially patterned structure and showed improved endothelial interaction compared to muscle engineered with randomly-oriented scaffolds.

    • Karina H. Nakayama
    • , Marco Quarta
    • , Patrick Paine
    • , Cynthia Alcazar
    • , Ioannis Karakikes
    • , Victor Garcia
    • , Oscar J. Abilez
    • , Nicholas S. Calvo
    • , Chelsey S. Simmons
    • , Thomas A. Rando
    •  & Ngan F. Huang
  • Q&A | | open

    Dr. Matt Field is a Senior Research Fellow in Bioinformatics at the Australian Institute of Tropical Health and Medicine at James Cook University, co-director of the Centre for Tropical Bioinformatics and Molecular Biology, and a Chief Investigator for the Centre for Personalised Immunology, an NHMRC Centre of Research Excellence. His research focuses on developing high-throughput bioinformatic analysis pipelines and bringing genomics and personalised medicine into routine clinical practice. In this instalment of our series on early-career researchers, we asked Dr. Field to talk about his research, career trajectory, and the importance of remembering the big picture when working on projects with potential clinical impact.

  • Article | | open

    Avery Paxton et al. test the effects of artificial reefs on abundance and biomass of fish on the southeastern coast of the USA. They report that deep artificial reefs are associated with high numbers and biomasses of tropical and subtropical fishes, likely caused by an increase in prey availability on artificial reefs.

    • Avery B. Paxton
    • , Charles H. Peterson
    • , J. Christopher Taylor
    • , Alyssa M. Adler
    • , Emily A. Pickering
    •  & Brian R. Silliman
  • Article | | open

    Alfred Pozarickij et al. present an analysis of effect-size heterogeneity among myopia-associated variants in the UK Biobank dataset. They find evidence for non-uniform effects for 45% of variants using a conservative cutoff, suggesting that gene-environment and/or gene-gene interactions are pervasive in myopia.

    • Alfred Pozarickij
    • , Cathy Williams
    • , Pirro G. Hysi
    • , Jeremy A. Guggenheim
    • , Tariq Aslam
    • , Sarah A. Barman
    • , Jenny H. Barrett
    • , Paul Bishop
    • , Peter Blows
    • , Catey Bunce
    • , Roxana O. Carare
    • , Usha Chakravarthy
    • , Michelle Chan
    • , Sharon Y.L. Chua
    • , David P. Crabb
    • , Philippa M. Cumberland
    • , Alexander Day
    • , Parul Desai
    • , Bal Dhillon
    • , Andrew D. Dick
    • , Cathy Egan
    • , Sarah Ennis
    • , Paul Foster
    • , Marcus Fruttiger
    • , John E.J. Gallacher
    • , David F. Garway-Heath
    • , Jane Gibson
    • , Dan Gore
    • , Chris J. Hammond
    • , Alison Hardcastle
    • , Simon P. Harding
    • , Ruth E. Hogg
    • , Pearse A. Keane
    • , Sir Peng T. Khaw
    • , Anthony P. Khawaja
    • , Gerassimos Lascaratos
    • , Andrew J. Lotery
    • , Tom Mac Gillivray
    • , Sarah Mackie
    • , Keith Martin
    • , Michelle McGaughey
    • , Bernadette McGuinness
    • , Gareth J. McKay
    • , Martin McKibbin
    • , Danny Mitry
    • , Tony Moore
    • , James E. Morgan
    • , Zaynah A. Muthy
    • , Eoin O’Sullivan
    • , Chris G. Owen
    • , Praveen Patel
    • , Euan Paterson
    • , Tunde Peto
    • , Axel Petzold
    • , Jugnoo S. Rahi
    • , Alicja R. Rudnikca
    • , Jay Self
    • , Sobha Sivaprasad
    • , David Steel
    • , Irene Stratton
    • , Nicholas Strouthidis
    • , Cathie Sudlow
    • , Dhanes Thomas
    • , Emanuele Trucco
    • , Adnan Tufail
    • , Veronique Vitart
    • , Stephen A. Vernon
    • , Ananth C. Viswanathan
    • , Katie Williams
    • , Jayne V. Woodside
    • , Max M. Yates
    • , Jennifer Yip
    •  & Yalin Zheng
  • Article | | open

    Tina Skinner-Adams et al. show that proguanil and the cyclization-blocked analogue, tBuPG, have potent, anti-plasmodial activity in vitro. This study suggests that a cyclization-blocked proguanil may be a superior combination partner for bc1-inhibitors to suppress the survival of malarial parasites.

    • Tina S. Skinner-Adams
    • , Gillian M. Fisher
    • , Andrew G. Riches
    • , Oliver E. Hutt
    • , Karen E. Jarvis
    • , Tony Wilson
    • , Mark von Itzstein
    • , Pradeep Chopra
    • , Yevgeniya Antonova-Koch
    • , Stephan Meister
    • , Elizabeth A. Winzeler
    • , Mary Clarke
    • , David A. Fidock
    • , Jeremy N. Burrows
    • , John H. Ryan
    •  & Katherine T. Andrews
  • Article | | open

    Wataru Kobayashi et al. use cryo-EM to study the C-terminal ELYS subunit of the nuclear pore complex. They show that a three-residue stretch binds to the acidic patch of the nucleosome, providing new mechanistic insights into ELYS nucleosome interactions.

    • Wataru Kobayashi
    • , Yoshimasa Takizawa
    • , Maya Aihara
    • , Lumi Negishi
    • , Hajime Ishii
    •  & Hitoshi Kurumizaka
  • Article | | open

    Jean-Bernard Caron and Brittany Cheung provide new evidence that the rare Cambrian animal Amiskwia is closely related to gnathiferans, thereby resolving its ambiguous phylogeny. They describe new specimens from the Burgess Shale that preserve the complex jaw apparatus, which is most similar to gnathostomulids.

    • Jean-Bernard Caron
    •  & Brittany Cheung
  • Article | | open

    Pawel Brzezowski et al. demonstrated existence of a link between chlorophyll biosynthesis and photosynthetic electron transport in Chlamydomonas reinhardtii. The lack of oxidised plastoquinone pool impairs function of PROTOPORPHYRINOGEN IX OXIDASE, leading to accumulation of non-metabolised protoporphyrin IX.

    • Pawel Brzezowski
    • , Brigitte Ksas
    • , Michel Havaux
    • , Bernhard Grimm
    • , Marie Chazaux
    • , Gilles Peltier
    • , Xenie Johnson
    •  & Jean Alric
  • Article | | open

    Jie Qiaoa, Wenqiang Lia, Siyu Lina et al. develop a cost-efficient method to prepare stable and efficient CRISPR/Cas ribonucleoprotein complexes. Using a one-step purification system with an ultra-high-affinity CL7/Im7 column they successfully harvested high yields of functional Cas9 and target-specific single guided RNAs co-expressed in Escherichia coli.

    • Jie Qiao
    • , Wenqiang Li
    • , Siyu Lin
    • , Wenli Sun
    • , Lixin Ma
    •  & Yi Liu
  • Article | | open

    Schiff et al. show that coactivation of TGFβ1 and TNFα causes aggressive contraction of human retinal pigment epithelial cells and increases expression of genes coding for muscle and extracellular rearrangement proteins. This pathological phenotype is reversed by p38 inhibition, highlighting its potential therapeutic role against proliferative vitreoretinopathy.

    • Lauren Schiff
    • , Nathan C. Boles
    • , Marie Fernandes
    • , Bar Nachmani
    • , Ronald Gentile
    •  & Timothy A. Blenkinsop
  • Article | | open

    Zohar et al. demonstrate a real-time imaging of fluorescently labeled cells embedded in 3D constructs subjected to different flow conditions. This study provides a framework that enables tracking cell activity continuously during tissue development, suggesting its potential utility for drug screens or cytotoxicity assays.

    • Barak Zohar
    • , Yaron Blinder
    • , Mark Epshtein
    • , Ariel A. Szklanny
    • , Ben Kaplan
    • , Netanel Korin
    • , David J. Mooney
    •  & Shulamit Levenberg
  • Article | | open

    Alborz Mazloomian et al. use small molecule inhibitors to disrupt EIF4A3’s ATPase and helicase function which affects alternative splicing and nonsense mediated decay of transcripts. They define a genome-wide pattern of motifs of RNA-binding proteins associated with EIF4A3 and find that stress granules are downregulated upon EIF4A3 inhibition.

    • Alborz Mazloomian
    • , Shinsuke Araki
    • , Momoko Ohori
    • , Amal M. El-Naggar
    • , Damian Yap
    • , Ali Bashashati
    • , Shoichi Nakao
    • , Poul H. Sorensen
    • , Atsushi Nakanishi
    • , Sohrab Shah
    •  & Samuel Aparicio
  • Article | | open

    Yasuhiko Matsumoto et al. show that E. faecalis YM0831 suppresses sucrose-induced hyperglycemia in silkworms. This bacterial strain also reduces glucose uptake in human Caco-2 cells and suppresses blood glucose levels in humans exposed to a sucrose tolerance test; suggesting a beneficial role for this lactic acid bacteria in sugar metabolism.

    • Yasuhiko Matsumoto
    • , Masaki Ishii
    • , Setsuo Hasegawa
    •  & Kazuhisa Sekimizu
  • Article | | open

    Adolfo Sequeira et al. report an analysis of GABA receptor subunit expression from single-cell and global transcriptome data generated by the Allen Institute. They find that GABA receptor subunit expression is spatially organized according to neurodevelopmental origin and generate a reference from the control population for future studies.

    • Adolfo Sequeira
    • , Kevin Shen
    • , Assaf Gottlieb
    •  & Agenor Limon
  • Article | | open

    Jeremy Linsley, Atmiyata Tripathi et al. optimise culturing techniques and develop a microscopy platform and that can automatically image and track single neurons within organotypic slices for up to a three-week period. Using a model of polyQ neurodegeneration, they show that this method can assess longitudinal cell changes with high accuracy.

    • Jeremy W. Linsley
    • , Atmiyata Tripathi
    • , Irina Epstein
    • , Galina Schmunk
    • , Elliot Mount
    • , Matthew Campioni
    • , Viral Oza
    • , Mariya Barch
    • , Ashkan Javaherian
    • , Tomasz J. Nowakowski
    • , Siddharth Samsi
    •  & Steven Finkbeiner
  • Article | | open

    Yanjian Li and Hailong Li, et al. report interspecific comparisons of the acetylomes of yeast and three human fungal pathogens. Through evolutionary analysis and experimental validation, they show that pathogen-specific aceylation motifs and deacetylase genes are required for normal virulence in an animal infection model.

    • Yanjian Li
    • , Hailong Li
    • , Mingfei Sui
    • , Minghui Li
    • , Jiamei Wang
    • , Yang Meng
    • , Tianshu Sun
    • , Qiaojing Liang
    • , Chenhao Suo
    • , Xindi Gao
    • , Chao Li
    • , Zhuoran Li
    • , Wei Du
    • , Baihua Zhang
    • , Sixiang Sai
    • , Zhang Zhang
    • , Jing Ye
    • , Hongchen Wang
    • , Shang Yue
    • , Jiayi Li
    • , Manli Zhong
    • , Changbin Chen
    • , Shouliang Qi
    • , Ling Lu
    • , Dancheng Li
    •  & Chen Ding
  • Article | | open

    Marsolier et al. show that a parasite-encoded prolyl-isomerase, TaPin1, stabilizes host pyruvate kinase isoform M2 (PKM2), leading to metabolically transformed phenotypes in parasite-infected cells. This study illustrates how intracellular parasites use prolyl isomerization to manipulate host metabolism to their advantage.

    • Justine Marsolier
    • , Martine Perichon
    • , Jonathan B. Weitzman
    •  & Souhila Medjkane
  • Article | | open

    Yusi Fu, Fangli Zhang et al. integrate a one-step microcapillary array with emulsion multiple displacement amplification to obtain a high-throughput single-cell whole-genome amplification method. Their method enables copy number assessment at a resolution of 50 kb.

    • Yusi Fu
    • , Fangli Zhang
    • , Xiannian Zhang
    • , Junlong Yin
    • , Meijie Du
    • , Mengcheng Jiang
    • , Lu Liu
    • , Jie Li
    • , Yanyi Huang
    •  & Jianbin Wang
  • Article | | open

    Nobuaki Kono et al. report the genome sequence of the bagworm, an insect that produces silk throughout all life stages. The authors find that bagworms have a novel fibroin gene sequence that contributes to the high tensile strength of bagworm silk.

    • Nobuaki Kono
    • , Hiroyuki Nakamura
    • , Rintaro Ohtoshi
    • , Masaru Tomita
    • , Keiji Numata
    •  & Kazuharu Arakawa
  • Article | | open

    Kanako Shimizu, Yusuke Sato et al. examine the impact of the Eomes transcription factor on invariant NKT (iNKT) cell differentiation using conditional knockout mice. They show that Eomes is needed for the development of iNKT1 cells in the thymus, and for their activation and differentiation in peripheral tissues.

    • Kanako Shimizu
    • , Yusuke Sato
    • , Masami Kawamura
    • , Hiroshi Nakazato
    • , Takashi Watanabe
    • , Osamu Ohara
    •  & Shin-ichiro Fujii
  • Article | | open

    Lacabanne and Orelle et al. investigate a multidrug ABC transporter BmrA mutant defective in the X-loop, which uncouples ATP hydrolysis from substrate translocation. This study suggests that stiffening of a defined set of amino-acid residues allows the pump to transport substrates properly.

    • Denis Lacabanne
    • , Cédric Orelle
    • , Lauriane Lecoq
    • , Britta Kunert
    • , Claire Chuilon
    • , Thomas Wiegand
    • , Stéphanie Ravaud
    • , Jean-Michel Jault
    • , Beat H. Meier
    •  & Anja Böckmann
  • Article | | open

    Priti Prasanna Maity et al. develop a method for high-yield isolation of type II collagen from the Capra hircus ear cartilage, a commonly available biowaste product. Using mass spectrometry they mapped the positions of hydroxyproline within the collagen II alpha chain; this methodology facilitates isolation and characterization of this biomedical resource.

    • Priti Prasanna Maity
    • , Debabrata Dutta
    • , Sayan Ganguly
    • , Kausik Kapat
    • , Krishna Dixit
    • , Amit Roy Chowdhury
    • , Ramapati Samanta
    • , Narayan Chandra Das
    • , Pallab Datta
    • , Amit Kumar Das
    •  & Santanu Dhara
  • Article | | open

    Xun Wang et al. show a ydaO riboswitch-regulated kdp operon expression in Bacillus thuringiensis BMB171. This work experimentally validates a long-suspected connection between the kdpFABC operon and the ydaO riboswitch identified on the genome of BMB171.

    • Xun Wang
    • , Xia Cai
    • , Hongdan Ma
    • , Wen Yin
    • , Li Zhu
    • , Xinfeng Li
    • , Heon M. Lim
    • , Shan-Ho Chou
    •  & Jin He
  • Article | | open

    Martinello et al. show that the KV7 K+ channels, active at rest, diminish action potential-induced Ca2+ influx into hippocampal mossy fiber boutons. This reduction alters presynaptic spikes, reduces neurotransmitter release, and decreases short-term synaptic plasticity, highlighting a role for these channels in hippocampal CA3 neuronal excitability.

    • Katiuscia Martinello
    • , Elisabetta Giacalone
    • , Michele Migliore
    • , David A. Brown
    •  & Mala M. Shah
  • Article | | open

    Johanna Michl et al. use real-time pH measurements of cell culture media and intracellular pH data from live-cell culture conditions to explore the effects of commonly-used buffers. Based on these data, they propose guidelines for controlling pH in cell culture systems and improving reproducibility.

    • Johanna Michl
    • , Kyung Chan Park
    •  & Pawel Swietach
  • Article | | open

    Cristina Zibetti et al. present ATAC-seq, RNA-seq and ChIP-seq analyses of murine retinal progenitors at early and late developmental stages. They find a central role for the LIM homeodomain transcription factor LHX2 in the global control of chromatin accessibility and identify Lhx2-dependent candidate pioneer factors that may contribute to the establishment and maintenance of open chromatin in the retina.

    • Cristina Zibetti
    • , Sheng Liu
    • , Jun Wan
    • , Jiang Qian
    •  & Seth Blackshaw
  • Article | | open

    Holly Vincent et al. use species distribution modeling, climate change projections, and geographical analyses of more than 1200 crop wild relatives, identifying 150 key conservation areas most effective in preserving genetic diversity of crop wild relatives.

    • Holly Vincent
    • , Ahmed Amri
    • , Nora P. Castañeda-Álvarez
    • , Hannes Dempewolf
    • , Ehsan Dulloo
    • , Luigi Guarino
    • , David Hole
    • , Chikelu Mba
    • , Alvaro Toledo
    •  & Nigel Maxted
  • Article | | open

    Jing Zhang et al. analyze glioblastoma patient data with a computational model integrating mutation-derived neoantigens similar to pathogen-specific antigens with enrichment of signatures for individual immune cells. Using a fitness model for identifying high-quality neoantigens and T lymphocyte infiltrates in glioblastoma, they uncover a subset of patients with prolonged survival.

    • Jing Zhang
    • , Francesca P. Caruso
    • , Jason K. Sa
    • , Sune Justesen
    • , Do-Hyun Nam
    • , Peter Sims
    • , Michele Ceccarelli
    • , Anna Lasorella
    •  & Antonio Iavarone
  • Article | | open

    Martin Brandt et al. studied foliage mass from Senegal over 30 years, observing the effects of climate change on dryland vegetation. Rainfall caused increases in both herbaceous and woody foliage, but woody vegetation benefits from rainfall timing changes with potential knock-on effects to the local herbivore populations.

    • Martin Brandt
    • , Pierre Hiernaux
    • , Kjeld Rasmussen
    • , Compton J. Tucker
    • , Jean-Pierre Wigneron
    • , Abdoul Aziz Diouf
    • , Stefanie M. Herrmann
    • , Wenmin Zhang
    • , Laurent Kergoat
    • , Cheikh Mbow
    • , Christin Abel
    • , Yves Auda
    •  & Rasmus Fensholt
  • Article | | open

    Josef Tkadlec et al. investigate population structures that mediate the trade-off between fixation probability and time. They identify structures that enable higher effective rates of evolution than the well-mixed population, which could be applied to in vitro evolution applications.

    • Josef Tkadlec
    • , Andreas Pavlogiannis
    • , Krishnendu Chatterjee
    •  & Martin A. Nowak