This Article contains errors in the Materials and Methods section under subheading ‘RNA Isolation and RT-PCR in zebrafish’.
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-CGAGGCGTTGATAAGCTCTCTGCT-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′and 5′-TTTACAGTGTGGGCT TGGAGGG-3′, Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATG ATGTCTACCA-3′.”
should read:
“One microgram of total RNA was reverse transcribed using MMLV reverse transcriptase and cDNA was amplified with the following primers: Danio rerio VEGF-A, 5′-GATGTGATTCCCTTCATGGATGTGT-3′ and 5′-GGATACTCCTGGATGATGTCTACCA-3′; Danio rerio β-actin, 5′-CAGCAAGCAGGAGTACGATGAGT-3′ and 5′-TTGAATCTCATTGCTAGGCCATT-3′; for the IL-8 gene, amplification of two different fragments was performed with two couples of primers, hereafter be referred to as a and b: Danio rerio IL-8a, 5′-AGCTTGAGAGGTCTGGCTGTAGA-3′ and 5′-GCGTCGGCTTTCTGTTTCA-3′, Danio rerio IL-8b, 5′-TGTTTTCCTGGCATTTCTGACC-3′ and 5′-TTTACAGTGTGGGCTTGGAGGG-3′.”
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The online version of the original article can be found at https://doi.org/10.1038/srep18785
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Pozzobon, T., Facchinello, N., Bossi, F. et al. Correction: Corrigendum: Treponema pallidum (syphilis) antigen TpF1 induces angiogenesis through the activation of the IL-8 pathway. Sci Rep 8, 46945 (2018). https://doi.org/10.1038/srep46945
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DOI: https://doi.org/10.1038/srep46945
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