In the original version of this Article, the Supplementary Software files were omitted. This has been corrected in the HTML version of the Article; the PDF version was correct at the time of publication.
The online version of the original article can be found at 10.1038/srep28680
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Mortensen, K., Flyvbjerg, H. Erratum: “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images. Sci Rep 7, 46883 (2017) doi:10.1038/srep46883
Journal of Biomedical Optics (2018)