Circadian locomotor behaviour is controlled by a pacemaker circuit composed of clock-containing neurons. To interrogate the mechanistic relationship between the molecular clockwork and network communication critical to the operation of the Drosophila circadian pacemaker circuit, we established new fluorescent circadian reporters that permit single-cell recording of transcriptional and post-transcriptional rhythms in brain explants and cultured neurons. Live-imaging experiments combined with pharmacological and genetic manipulations demonstrate that the neuropeptide pigment-dispersing factor (PDF) amplifies the molecular rhythms via time-of-day- and activity-dependent upregulation of transcription from E-box-containing clock gene promoters within key pacemaker neurons. The effect of PDF on clock gene transcription and the known role of PDF in enhancing PER/TIM stability occur via independent pathways downstream of the PDF receptor, the former through a cAMP-independent mechanism and the latter through a cAMP-PKA dependent mechanism. These results confirm and extend the mechanistic understanding of the role of PDF in controlling the synchrony of the pacemaker neurons. More broadly, our results establish the utility of the new live-imaging tools for the study of molecular-neural interactions important for the operation of the circadian pacemaker circuit.
Orchestrated action of genetic programs and neuronal activity determines the emergent output of a neural circuit. The control of circadian behavioural rhythms is a prime example of this orchestration. Networks of dedicated pacemaker neurons, each containing a molecular clock, produce synchronized daily rhythms in gene expression and electrical activity, thereby driving coherent rhythmic behaviour. Owing to its numerically simpler nervous system and a battery of genetic tools, Drosophila offers an unusually powerful model to dissect the molecular and neural mechanisms of circadian behaviour.
The molecular clock of Drosophila consists of interlocked transcriptional-translational feedback loops, as in vertebrates. In the core feedback loop of the Drosophila clock, transcription factors CLOCK (CLK) and CYCLE (CYC) heterodimerize and activate transcription of period (per) and timeless (tim) through binding to their upstream and intronic E-boxes. Following the translation of per and tim mRNAs, PER and TIM translocate into the nucleus and inhibit CLK/CYC-mediated transcription. The CLK/CYC heterodimer also rhythmically activates transcription of a large number of output genes by binding rhythmically to their E-boxes, thereby controlling circadian rhythms in cellular functions1. Along with these transcriptional mechanisms, multiple post-transcriptional and post-translational mechanisms, particularly the post-translational modifications of PER that control its timed nuclear localization and degradation, are critical for the generation of circa 24-hr period rhythms2.
The circadian pacemaker circuit is composed of approximately 150 clock-containing neurons in the brain and controls the circadian locomotor rhythms of adult flies. Clock neurons are classified into 7 major subgroups: two classes of ventral lateral neurons (small-ventral lateral neurons, s-LNvs; large-ventral lateral neurons, l-LNvs), dorsal lateral neurons (LNds), lateral posterior neurons (LPNs), and three classes of dorsal neurons (DN1s, DN2s, and DN3s). Four out of five s-LNvs and all the l-LNvs express the neuropeptide Pigment-dispersing factor (PDF). The larval circadian circuit is composed of three classes of clock neurons (LNvs, DN1s and DN2s) and controls circadian light response behavior3,4. Larval PDF-positive LNvs are identical to the adult PDF-positive s-LNvs, which are the master pacemakers that control circuit synchrony and coordinate behavioural output5.
Previous studies have demonstrated the contribution of neuronal excitability and network interactions to rhythm generation in Drosophila pacemaker clock neurons. Similar to what is observed in the mammalian circadian pacemaker neurons located in the suprachiasmatic nuclei (SCN)6,7, continuous neuronal silencing and blockage of synaptic transmission do not abolish the molecular clockwork in Drosophila clock neurons, although these manipulations impair behavioural output8,9. A number of studies have shown that PDF plays critical roles in controlling synchrony in clock neurons and behavioural rhythms, analogous to the roles of vasoactive intestinal peptide (VIP) in the SCN10,11,12. PDF acts on multiple clock neuron subtypes through possibly diverse and cell type-specific mechanisms of action, e.g., controlling the phase, amplitude or pace of molecular clocks13,14,15,16,17,18,19. A recent study also demonstrated that PDF is required to control the phase of clock neuron output20. However, despite these advances in characterizing the role of PDF signalling in the circadian circuit, a mechanistic understanding of the precise interplay between the molecular clock and PDF signalling requires further investigation. Other neuropeptides and neurotransmitters are also involved in circuit synchronization and the coordination of behavioural output21,22; however, their impact on the molecular clockwork remains largely uncharacterized.
Precise mechanistic analysis of the interplay between network interactions and the molecular clockwork requires a system that enables the manipulation of network communication while simultaneously monitoring the dynamics of the molecular clock. To this end, here we develop two novel fluorescent reporters that permit real-time recording of the transcriptional and post-transcriptional machineries of the Drosophila clock in cultured neurons and whole brains. Using these new tools combined with genetic and pharmacological manipulations, we demonstrate that PDF enhances CLK/CYC-mediated transcription cell-autonomously at night via an activity-dependent, cAMP-independent mechanism. Our results confirm and extend the role of PDF in the molecular clock and more broadly demonstrate the utility of the novel live-imaging tools for investigating the molecular-neural interactions important for the operation of the circadian pacemaker circuit.
Novel circadian reporters for live fluorescence imaging
Transcriptional activation from E-box-containing promoters and the feedback transcriptional inhibition by PER comprise the essential core loop of the molecular clock. Post-transcriptional regulatory features that control the stability, activity and subcellular localization of PER are also critical for the generation of circa 24-hr period rhythms23. Therefore, to monitor the functioning of the transcriptional and post-transcriptional machinery of the molecular clock at single-cell resolution, we generated two fluorescent reporters: one to monitor CLK/CYC transcriptional activity rhythms and the other to follow PER protein levels and localization (Fig. 1a).
The CLK/CYC transcriptional activity reporter 3 × 69-VNP expresses short-lived yellow fluorescent protein fused to a nuclear localization signal (VENUS-NLS-PEST, VNP)24 under the control of three tandem repeats of the clock regulatory sequence (CRS), which is the E-box-containing 69-bp enhancer of per. The CRS is necessary and sufficient for per-like spatial and temporal transcriptional expression25, and the CRS trimer has been shown to drive CLK/CYC-dependent, high-intensity and high-amplitude circadian reporter expression in vivo26. The PER protein reporter per-TdT was modified from the previously established BG-luc reporter27 and was designed to express the N-terminal two-thirds of PER fused with tandem TOMATO (TdT) red fluorescent protein, under the control of per regulatory sequences and the 3′UTR. The N-terminal PER moiety harbours most of the known functional domains, including two PER-ARNT-SIM (PAS) domains and an NLS sequence, but it lacks the CLK/CYC inhibition domain and thus does not replace endogenous PER function27.
As expected, both molecular clock reporters were expressed rhythmically in adult and larval brain clock neurons (Fig. 1b,c, and S1a–d). A detailed analysis of the simple circadian circuit of third-instar larvae revealed that 3 × 69-VNP peaked between ZT14 (2 hr after lights off under a 12 hr:12 hr light-dark (LD) cycle) and ZT18, which corresponds approximately to the peak phases of per mRNA accumulation, CLK/CYC binding to per E-boxes, and genome-wide CLK/CYC binding1,28,29. PER-TDT peaked approximately 8 hr after the 3 × 69-VNP peak in the LNvs and DN1s, recapitulating the characteristic lag between per mRNA and protein accumulation29. PER-TDT rhythms in the DN2s were anti-phasic to the LNvs and DN1s, which is consistent with endogenous PER cycling30 (Fig. 1c and S1a). Furthermore, the cycling profile of PER-TDT nuclear-cytoplasmic localization was identical to that of PER (Fig. 1d)31. The cycling expression of both reporters and the rhythms in PER-TDT nuclear/cytoplasmic ratio were largely maintained in DD (Fig. S1c and d). These results establish that the 3 × 69-VNP and per-TdT reporters provide reliable readouts of the molecular clockwork at a single-cell resolution; 3 × 69-VNP reports CLK/CYC-mediated transcriptional rhythms and also mimics per mRNA rhythms, whereas PER-TDT mimics PER protein oscillations.
Next, we tested whether our new reporters can be also used for monitoring rhythms in live preparations. Cultured whole brains of adult flies have been shown to generate rhythms in luciferase reporter activity in a tissue-autonomous manner32,33,34. We chose to use cultured third-instar larval brains for live imaging, as larval circadian circuits are numerically simpler yet composed of fully differentiated and functional clock neurons35. As expected, clock neurons in cultured larval brains displayed circadian rhythms in 3 × 69-VNP and PER-TDT reporter expression, although with a longer average period (~29 hr) (Fig. 2a–c, Movies S1 and S2). Each neuron expressed varying levels of fluorescence; thus, not all the neurons were detected. Among the detected neurons, 57% of the LNvs, 50% of DN1s and 76% of DN2s showed circadian rhythms in 3 × 69-VNP levels, and 75% of the LNvs showed rhythmic PER-TDT expression. Since the long periods were likely due to the combination of the longer endogenous rhythms of the reporter flies (free-running locomotor rhythms of 1982Clk > mCD8::RFP, 3 × 69-VNP flies: 25.1 ± 0.13 hr; gal1118 > mCD8::Venus, per-TdT flies: 25.3 ± 0.08 hr), the 3 hr temporal resolution and culture conditions, we limited the use of brain explants to relatively short (<8 hr) fluorescent live-imaging at 1 hr intervals in subsequent experiments.
PDF enhances CLK/CYC-mediated transcription at night
The neuropeptide PDF mediates normal circadian locomotor rhythms by affecting the synchrony, amplitude and pace of the molecular clocks15,16,18,19 and by coordinating the phase of pacemaker neuron activity20. PDF activates adenylate cyclase and increases cAMP levels in PDF receptor (PDFR)-expressing neurons36,37,38. The rise in cAMP levels activates protein kinase A (PKA) and stabilizes TIM and PER14,17, which contributes to the phase resetting and speed control of the molecular clocks. PDF also enhances neuronal activity in a cell-autonomous manner36,39,40. Importantly, the stabilization of TIM via PDF signalling is activity-independent; therefore, PDF/PDFR signalling at least bifurcates into the pathway controlling neuronal excitability and the one controlling PER/TIM stability17. However, the possible effects of PDF signalling on clock gene transcription have not been directly examined.
To better dissect the effect of PDF signalling on the clockwork, we monitored 3 × 69-VNP and PER-TDT expression in larval brain explants following a bath application of PDF. LD-entrained larvae were dissected at ZT1 or ZT13 and immediately subjected to live imaging in DD. PDF was applied at ZT2 or ZT14 (i.e., 1 hr after the start of live imaging). PDF application at ZT2 did not alter 3 × 69-VNP or PER-TDT expression in any larval clock neuron subtype. In contrast, PDF application at ZT14 upregulated 3 × 69-VNP and PER-TDT levels in the LNvs and DN1s. PDF application did not detectably affect reporter expression in the DN2s (Fig. 3a and b). Reporter expression levels continued to rise in the LNvs and DN1s during the course of imaging following the application of PDF at ZT14. This is consistent with the previous finding by Klose et al. that signalling downstream of PDFR continues to be active as long as PDF is present in the medium in ex vivo preparations41. As the addition of PDF had no effect on the expression profiles of 3 × 69-VNP and PER-TDT in cultures prepared from the pdfr hypomorphic mutant larvae, even at ZT14, the effect of PDF is specific and occurs via PDFR (Fig. S2a). Furthermore, PDF upregulated 3 × 69-VNP and PER-TDT levels at ZT14 but not at ZT2 also in pdf01 mutants (Fig. S3). These results indicate that PDF upregulates CLK/CYC-mediated transcription and PER levels in the LNvs and DN1s at night but not during the day, and this nighttime-specific response of the molecular clock is controlled independently of the timing of PDF release.
To test whether neuronal excitability is involved in the reporter upregulation induced by PDF at ZT14, we co-applied the voltage-gated sodium channel blocker tetrodotoxin (TTX) and PDF to the brain explants. PER-TDT upregulation by PDF was insensitive to TTX (Fig. 3b). This finding parallels the observation that PDF stabilizes TIM through a mechanism independent of neuronal activity17. In contrast, TTX blocked the PDF-mediated upregulation of 3 × 69-VNP (Fig. 3a), indicating that PDF affects CLK/CYC-mediated transcription via time-of-day- and activity-dependent mechanisms.
To further test if the upregulation of PER-TDT by PDF is mediated by PKA activation14, we applied the cAMP analogue Sp-adenosine-3′, 5′- cyclic monophosphorothioate triethyamine (Sp-cAMPS), which specifically activates PKA42, to the larval brain explants at ZT14. Sp-cAMPS did not affect 3 × 69-VNP levels but significantly increased PER-TDT levels (Fig. 4), consistent with the role of cAMP-PKA signalling in PER stabilization14. Taken together, these results demonstrate that PDF/PDFR-signalling modulates the molecular clockwork specifically at night via two pathways: one pathway involving an increase in CLK/CYC-mediated transcription in an activity-dependent mechanism and the other involving enhancement of PER stability through cAMP-PKA activation, independent of neuronal excitability.
Cell-autonomous, activity-dependent upregulation of clock gene transcription by PDF
TTX blocks spontaneous firing generated by cell-intrinsic mechanisms as well as the action potentials triggered by synaptic inputs43,44. To distinguish between these two possibilities, we monitored 3 × 69-VNP expression in dissociated cultured neurons in real time. Cultures were prepared from LD-entrained larvae expressing 3 × 69-VNP and the clock neuron marker (1982clk-gal4, UAS-mCD8::RFP) at approximately ZT11, then incubated for two days in DD and then subjected to time-lapse imaging starting at CT18. The cultured neurons were morphologically intact, and their neurites continued to grow and elaborate as previously shown45, even during the time-lapse imaging (Fig. S2b).
All of the 1982clk > mCD8::RFP clock neuron marker-positive cells co-expressed 3 × 69-VNP; however, 3 × 69-VNP levels showed steady increases over time without detectable rhythms. Remarkably, the addition of PDF caused a significant further increase in 3 × 69-VNP levels (Movie S3, Fig. 5a and d). The addition of PDF had no significant effect on 3 × 69-VNP expression in the culture prepared from the pdfr mutant larvae (Fig. 5b and e), confirming that the effect of PDF in vitro is specific and via PDFR, as in brain culture. Furthermore, TTX or the inhibitory neurotransmitter GABA completely inhibited the upregulation of 3 × 69-VNP levels in response to acute PDF treatment (Fig. 5c and d). These results indicate that PDF upregulates CLK/CYC-mediated transcription by a cell-autonomous, activity-dependent mechanism.
Bioluminescence-based circadian reporters allow non-invasive, long-term recording with a high temporal resolution and have been successfully used to monitor rhythmicity in real time32,33,34. In contrast, fluorescent reporters can achieve the higher spatial resolution necessary to distinguish individual cells in a cluster and even to characterize intracellular protein dynamics. Using two new fluorescent reporters of the Drosophila molecular clock, we were able to separately interrogate two important aspects of the clockwork: CLK/CYC-mediated transcription from E-boxes and PER intracellular dynamics, both at a single-cell resolution and in real time.
Our study confirms and extends the mechanistic links between network interactions and molecular clockworks, particularly those mediated by PDF signalling. PDF has been shown to trigger multiple downstream effects even within the same neuron. We showed that PDF bath application upregulates PER-TDT in whole-brain cultures, consistent with previous findings indicating that PDF stabilizes PER and TIM via PKA activation independently of neuronal activity14,17. In addition, we demonstrated for the first time that PDF also enhances CLK/CYC-mediated transcription through a cell-autonomous, activity-dependent but cAMP-PKA signalling-independent mechanism. Furthermore, the effect of PDF on PER stability and clock gene transcription occurs at night but not during daytime (Figs 3,4 and 6).
PDF signalling occurs via both cAMP-dependent and cAMP-independent pathways in cockroach circadian pacemaker neurons to increase the intracellular Ca2+ concentration40. Similarly, we demonstrated here the presence of cAMP-independent pathway in Drosophila PDF signalling, which leads to the activation of clock gene transcription (Fig. 4a). Activity-dependent gene expression triggered by increases in Ca2+ concentration is a widespread phenomenon in the nervous system46. Although it is beyond the scope of this study to identify the mechanisms by which PDF leads to the activity-dependent (TTX-sensitive) upregulation of CLK/CYC-transcription, we speculate that Ca2+ influx downstream of PDF/PDFR signalling leads to an upregulation of clock gene transcription. As implied by Agrawal et al.47, PDFR-dependent increase in Ca2+ concentration may be mediated by IP3/Ca2+ signalling.
How does PDF confer its time-of-day-dependent effects on the molecular clock? PDF rhythmically accumulates at the s-LNv dorsal termini with a peak around ZT248, and is predicted to be released at a higher rate at ZT2 than at ZT1449. However, our observation of ZT14-specific reporter upregulation even in the absence of endogenous PDF (Fig. S3) indicates that intracellular timing mechanisms (but not the rhythms in PDF availability) gate the molecular clock transcriptional and post-transcriptional responses to PDF. Rhythms in PDFR expression might be a possible mechanism of time-of-day-dependent PDF response, although the precise daily patterns of PDFR expression are yet to be characterized. Klose et al. showed that the sensitivity of the s-LNvs to PDF, measured based on the cAMP concentration, exhibits daily rhythms and peaks at dawn41. This suggests that cAMP signalling is permissive for the PER stabilization effect of PDF even at its nadir and the mechanisms downstream of cAMP production gate the PER stabilization effect at night.
The electrical activity of clock neurons is rhythmic and highest from the late night to early morning49,50,51. As intracellular Ca2+ levels in the s-LNvs and DN1s peak at approximately the same time20, rhythms in neuronal excitability and Ca2+ may gate the transcriptional response to PDF. We showed that TTX inhibits the nighttime-specific upregulation of clock gene transcription via PDF in brain explant cultures (Fig. 3a). These results suggest the possibility that synaptic inputs to the LNvs and DN1s control the neuronal activity relevant for gating the transcriptional response to PDF. On the other hand, PDF addition to dissociated neurons uniformly triggered TTX-sensitive transcriptional upregulation despite the lack of rhythms (Fig. 5a,c and d). This result suggests that the transcriptional upregulation induced by PDF may be the default state, i.e., that the clock may negatively regulate clock gene transcription unresponsive to PDF in the morning. As TTX can also inhibit spontaneous firing occurring independently of synaptic inputs, these results also imply that cell-autonomous control of neuronal firing rhythms by the molecular clock may be involved in the mechanisms gating the response to PDF. Fluorescent circadian reporter imaging combined with neuronal silencing or excitation by genetic tools will facilitate further studies to investigate whether the neuronal activity relevant for the response to PDF is mediated by circuit properties or by cell-autonomous mechanisms.
The nighttime-specific effect of PDF on clock gene transcription may be related to the mechanism of phase shift. Light increases the firing rate of pacemaker neurons52 and likely induces PDF release from the LNvs. It has also been shown that acute induction of firing mimics the effect of light in phase shift12. Therefore, an increase in PDF release in the early night by a light pulse when PDF levels should normally be low may trigger phase delay via enhanced clock gene transcription. Our finding that PDF-mediated activation of clock gene transcription is activity-dependent (Figs 3a and 5) also supports this possibility.
The time-of-day-dependent modulation of the molecular clockwork by PDF may also enhance the synchronization of clock neurons. CLK/CYC-transcription is active during the late day-early night and reaches a maximum around ZT14. PER maximally accumulates somewhat later, in the middle or near the end of the night23. As reported previously14, the effect of PDF on PER stabilization likely occurs in the late night to slow down the pace of intrinsically fast-paced clock neurons, such as the s-LNvs and DN1a (same as larval LNvs and DN1s)19. Furthermore, by stimulating CLK/CYC-mediated transcription in the early night, PDF signalling can amplify molecular clock oscillations, which contributes to phase synchrony (Fig. 6). Interestingly, a computational study predicts that VIP, the functional ortholog of PDF, can mediate synchrony across SCN neurons by inducing per expression only if VIP signalling occurs in-phase with per transcription53. Our results strikingly parallel this model and suggest that neuropeptide signalling in-phase with core clock gene transcription is a conserved principle for achieving pacemaker neuron synchrony.
It is noteworthy that reporter upregulation upon PDF application to brain explants was observed in the LNvs and DN1s but not in the DN2s (Figs 3 and 4). Adult DN2s express PDFR and can be activated by PDF38,54. Although larval DN2s also express PDFR30, the signalling downstream of PDFR in these cells may differ from that in the LNvs and DN1s. This finding underscores that PDF has multiple cell-type-specific roles.
One of our most intriguing observations is the lack of discernible circadian rhythms in 3 × 69-VNP expression in dissociated neuron cultures (Fig. 5). This finding suggests a possibility that robust transcriptional rhythms may require intact network communication and parallels the notion that circuit properties strongly influence the robustness of the mammalian SCN clocks6,55.
A recent study by Mezan et al. from Kadener’s group used another fluorescent circadian transcriptional reporters and showed that PDF signalling negatively regulates clock gene expression56. The differences in experimental setup and timescale of observations likely contributed to the differences in the conclusions between our and their studies. Whereas we focused on analysing acute response to PDF, Mezan et al. investigated the effect of pdfr mutation on tim transcription at steady-state or following the induction of CLK-GR transgene nuclear localization to increase CLK/CYC-mediated transcription. Before induction, CLK-GR acts dominant negatively to inhibit clock gene transcription, and it takes at least 24 hr to observe the clock gene tim transcriptional upregulation after CLK-GR induction. It will be interesting to compare short- and long-term effect of PDF on clock gene transcription using both experimental systems in future studies.
Cell type-specific differences in PDF signalling, downstream molecular components and relationships with neuronal activity are important issues to be addressed in future studies. There are likely other important neuropeptides and neurotransmitters that link neuronal communication with the molecular clockwork. Our new fluorescent reporters can be combined with optogenetic tools and genetically encoded calcium reporters to manipulate and analyse neuronal activity; however, the period estimated from the fluorescence time-lapse imaging could be imprecise due to its inherent lower temporal resolution compared with bioluminescence recordings. As is often the case in any Drosophila transgenic line, we also observed that genetic background affects the period length of the reporter lines independently of the copy number of the fluorescent reporters. Our results also suggest that culture conditions of brain explants impact the rhythmicity and period of the reporters. Given these advantages and caveats, the application of our fluorescent reporters to imaging in intact fly brains20,57 may offer powerful tools to further decipher the intricate interactions between neuronal signalling and the molecular clockwork.
Drosophila were reared at 25 °C on a corn-meal medium under 12 hr:12 hr light-dark (LD) cycles. The 1982clk-gal4 line was provided by N.R. Glossop58. The GAL4 enhancer trap line gal111859, and pdfr5304 60, pdf01 (Bloomington stock center, nb 26654) have been previously characterized.
UAS-mCD8::VENUS and UAS-mCD8::RFP constructs were generated by exchanging the GFP coding sequence of the pUAST/mCD8-GFP construct provided by L. Luo6161 to Venus cDNA or mRFP cDNA. The resulting constructs were introduced into the genome by P-element-mediated germline transformation.
To generate 3 × 69-VNP flies, the hs43 basal promoter sequence from pCaSpeR-hs43-lacz (GenBank accession number X81643), the Venus-NLS-PEST1 coding sequence24 and 1 kb of the per 3′ UTR were PCR-amplified and ligated into the vector containing three tandem copies of the 69-bp CRS of per (pCaSpeR-per69 × 3-luc), thus replacing the luciferase coding sequence and the SV40 3′ UTR26. The resulting pCaSpeR-3 × 69-Venus-UTR vector was used for P-element-mediated germline transformation.
To generate the per-TdT transgenic line, the pStinger vector was modified by removing the coding sequences of 5xUAS and EGFP-NLS and adding the attB sequence between the P-element 3′ end and a gypsy insulator. A 13.2 kb per genomic fragment (from the BamHI site at −4.2 kb to the EcoRI site located ca. 2 kb downstream of exon 8) was cloned into a modified pStinger vector. The tdTomato (TdT) coding sequence, amplified from the pRSET-B/tdTomato plasmid (generated by R. Tsien and provided by L. Luo), and a 1 kb per 3′UTR sequence were cloned into the pBS plasmid. A BamHI-PsiI fragment from this plasmid containing the TdT-3′UTR was then ligated to the BamHI-PsiI fragment of pStinger-per13.2, which resulted in the per (exon 1–5)-TdT-3′UTR fusion construct (per-TdT vector). The per-TdT strains were generated by integrating the per-TdT vector into the attP16 (2R) and attP40 (2L) landing sites by PhiC13-mediated site-specific integration. Injections were performed by BestGene Inc. The per-TdT (attP16) and per-TdT (attP40) lines were crossed to recombine both transgenes on the 2nd chromosome.
Larval brain culture
The brains of LD-entrained non-wandering L3 larvae were dissected in ice-cold saline solution62 at ZT11. The imaginal discs were left intact to prevent any tearing of the tissue. Dissected brains were kept on ice in modified Schneider’s medium (SMactive)63 supplemented with 5 mM Bis-Tris (Sigma). For ex vivo brain culture, the brain explants were mounted on a glass-bottom dish (35 mm MatTek petri dish, 20 mm microwell with 0.16/0.19 mm coverglass) in a fibrinogen clot prepared by adding thrombin (bovine thrombin, Sigma) to fibrinogen (bovine fibrinogen, Calbiochem) as previously described64. The glass-bottom well was filled with the SMactive medium and covered by a Teflon membrane permeable to oxygen. The cultured brains were kept at 25 °C in 80% relative humidity and in the dark for a few hours prior to time-lapse imaging to allow the brains to set. Time-lapse imaging was performed in the same culture conditions, with images acquired every 3 hr.
For pharmacological experiments on brain explants, brains were dissected either at ZT1 or at ZT13 and cultured in dissecting saline solution (equivalent to hemolymph-like saline) without a Teflon membrane. The brain explants were immediately imaged to establish a baseline. Approximately 1 hr after the start of the time-lapse imaging, the neuropeptide PDF (custom-made by Chi Scientific, H-NSELINSLLSLPKNMNDA-OH; 2 μM) was added alone or in combination with 100 nM TTX (Cayman Chemical). For the control condition, the vehicle (DMSO) was added. Sp-cAMPS (sc-201571 from Santa Cruz Biotechnology, 150 μM) was added alone and the vehicle (H2O) was used as a control. Time-lapse imaging was performed in the same conditions as described above with images acquired every hour for 8 hr.
Primary neuron culture
Dissociated neuron culture was performed as previously described45,64 with the following modifications. In brief, the dissected brains were enzymatically treated with 50 units/mL papain (Worthington) and mechanically dissociated. The cell suspension was then plated on glass-bottom dishes (35 mm MatTek petri dish, 10 mm microwell with 0.16/0.19 mm coverglass) coated with concanavalin A (Sigma). Once the cells were attached to the glass bottom, the dish was flooded with SMactive. The dissociated neuron culture was incubated at 25 °C in 80% relative humidity and constant darkness for 2 days prior to time-lapse imaging. For pharmacological experiments, 2 or 20 μM PDF, 100 nM TTX, 10 μM GABA (Sigma), or the vehicle (DMSO or ddH2O) was added to the cell culture medium just before the start of the time-lapse imaging. Time-lapse experiments were conducted in the same culture conditions, and the images were captured every 3 hr.
Microscopy and image analysis
A Leica TCS SP5 tandem scanner confocal microscope was used for fluorescence imaging. The same parameter settings were used to image all samples of the same type (dissected brains, cultured brains or cultured neurons). Freshly dissected larval and adult brains were scanned using a 40x water-immersion objective with the galvo scanner at 400 Hz. For time-lapse imaging, 20x, 40x or 63x objectives were used, and the images were acquired using the resonant scanner at 8,000 Hz with high-sensitivity HyD detectors. A bi-directional scan was used together with an 8x line average. Z-section steps of 1.7 μm × 8 and 2 μm × ~40 were used for imaging the dissociated cultured neurons and ex vivo brain culture, respectively. A 514-nm laser was used to excite the VENUS fluorophore (0.68 μW/cm2 to image VNP, 0.40 μW/cm2 for mCD8::VENUS), and a 561-nm laser was used for the TdTomato and mRFP fluorophores (14.8 μW/cm2 for PER-TDT, 5.71 μW/cm2 for mCD8::RFP). The laser intensity was measured at the level of the sample with a microscope slide power meter (Thorlabs, S170C).
The raw data of the larval and adult brain images taken at different time points and the time-lapse movies of the ex vivo brain cultures were analysed with FIJI software65. Briefly, a SUM-stack containing the clock neuron cluster of interest was constructed, and the area of each neuron was manually determined. The mean fluorescence intensity of the defined area in the SUM-stack was measured. Three nearby areas were also measured to analyse the background fluorescence level. The corrected total relative intensity of each cell was calculated as follows:
The fluorescence intensities in the time-lapse movies of the cultured neurons were measured using Imaris software (Bitplane). A 3D mask (region of interest in 3D) was built for each cell by thresholding the 3 × 69-VNP or PER-TDT fluorescence levels after background subtraction. The intensity SUM in each 3D mask was then extracted from the statistical data that were automatically generated by the program. For presentation purposes only, some of the images and time-lapse movies were processed with a 10x iterative deconvolution using AutoQuant (MediaCybernetics) and Imaris. When necessary, time-lapse images were also treated for 3D correction drift using the ImageJ plugin (NIH).
Time series data analysis
Fluorescence-intensity time-series data were normalized to the value at t = 0. Heatmaps representing the fluorescence-intensity time course were generated with an in-house R script. Each row represents the data of a single cell. Rows were ordered by the highest intensity over 24 hr.
For rhythm analysis, a combination of manual inspection and Maximum Entropy Spectral Analysis (MESA) was used to detect rhythmicity and estimate the period. MESA was chosen because, unlike other methods, it is adapted for the detection of rhythms in short or noisy time series66. First, time course plots of the intensity were generated using Excel or Prism (GraphPad Prism version 6.0c for Mac, GraphPad Software, San Diego, California, USA, www.graphpad.com). Because we found that 6th-order polynomial regression models best fit the data, we superimposed the 6th-order polynomial trend lines on the graphs to facilitate the detection of rhythmicity. The time-series data with circadian rhythms, defined by a peak-to-peak interval from 18 to 36 hr, were then identified by manual inspection. However, the polynomial trend lines were not used to determine the period. In parallel, the intensity time-series data without normalization were analysed with MESA without filtering. The time-series data were scored as circadian only when both the manual and MESA analyses detected the rhythms with a period between 18 and 36 hr.
Statistical analyses were performed with GraphPad Prism software. To compare the effects of the drug treatments in the pharmacological experiments (Figs 3–5), a two-way ANOVA with a Sidak test to correct for multiple comparisons was used.
How to cite this article: Sabado, V. et al. Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock. Sci. Rep. 7, 41560; doi: 10.1038/srep41560 (2017).
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Abruzzi, K. C. et al. Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. Genes Dev 25, 2374–2386 (2011).
Hardin, P. E. Molecular genetic analysis of circadian timekeeping in Drosophila. Adv Genet 74, 141–173 (2011).
Malpel, S., Klarsfeld, A. & Rouyer, F. Circadian synchronization and rhythmicity in larval photoperception-defective mutants of Drosophila. J Biol Rhythm 19, 10–21 (2004).
Mazzoni, E. O., Desplan, C. & Blau, J. Circadian pacemaker neurons transmit and modulate visual information to control a rapid behavioral response. Neuron 45, 293–300 (2005).
Nitabach, M. N. & Taghert, P. H. Organization of the Drosophila circadian control circuit. Curr Biol 18, R84–93 (2008).
Webb, A. B., Angelo, N., Huettner, J. E. & Herzog, E. D. Intrinsic, nondeterministic circadian rhythm generation in identified mammalian neurons. Proc Natl Acad Sci USA 106, 16493–16498 (2009).
Yamaguchi, S. et al. Synchronization of cellular clocks in the suprachiasmatic nucleus. Science 302, 1408–1412 (2003).
Depetris-Chauvin, A. et al. Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs. Curr Biol 21, 1783–1793 (2011).
Kaneko, M., Park, J. H., Cheng, Y., Hardin, P. E. & Hall, J. C. Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms. J Neurobiol 43, 207–233 (2000).
Hastings, M. H., Brancaccio, M. & Maywood, E. S. Circadian pacemaking in cells and circuits of the suprachiasmatic nucleus. J Neuroendocrinol 26, 2–10 (2014).
Shafer, O. T. & Yao, Z. Pigment-Dispersing Factor Signaling and Circadian Rhythms in Insect Locomotor Activity. Curr Opin Insect Sci 1, 73–80 (2014).
Guo, F., Cerullo, I., Chen, X. & Rosbash, M. PDF neuron firing phase-shifts key circadian activity neurons in Drosophila. Elife 3 (2014).
Collins, B. et al. Differentially timed extracellular signals synchronize pacemaker neuron clocks. PLoS Biol 12, e1001959 (2014).
Li, Y., Guo, F., Shen, J. & Rosbash, M. PDF and cAMP enhance PER stability in Drosophila clock neurons. Proc Natl Acad Sci USA 111, E1284–1290 (2014).
Lin, Y., Stormo, G. D. & Taghert, P. H. The neuropeptide pigment-dispersing factor coordinates pacemaker interactions in the Drosophila circadian system. J Neurosci 24, 7951–7957 (2004).
Peng, Y., Stoleru, D., Levine, J. D., Hall, J. C. & Rosbash, M. Drosophila free-running rhythms require intercellular communication. PLoS Biol 1, E13 (2003).
Seluzicki, A. et al. Dual PDF signaling pathways reset clocks via TIMELESS and acutely excite target neurons to control circadian behavior. PLoS Biol 12, e1001810 (2014).
Wulbeck, C., Grieshaber, E. & Helfrich-Forster, C. Pigment-dispersing factor (PDF) has different effects on Drosophila’s circadian clocks in the accessory medulla and in the dorsal brain. J Biol Rhythms 23, 409–424 (2008).
Yoshii, T. et al. The neuropeptide pigment-dispersing factor adjusts period and phase of Drosophila’s clock. J Neurosci 29, 2597–2610 (2009).
Liang, X., Holy, T. E. & Taghert, P. H. Synchronous Drosophila circadian pacemakers display nonsynchronous Ca(2)(+) rhythms in vivo. Science 351, 976–981 (2016).
Beckwith, E. J. & Ceriani, M. F. Communication between circadian clusters: The key to a plastic network. Febs Letters 589, 3336–3342 (2015).
Yao, Z. & Shafer, O. T. The Drosophila Circadian Clock Is a Variably Coupled Network of Multiple Peptidergic Units. Science 343, 1516–1520 (2014).
Hardin, P. E. & Panda, S. Circadian timekeeping and output mechanisms in animals. Curr Opin Neurobiol 23, 724–731 (2013).
Nagoshi, E. et al. Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells. Cell 119, 693–705 (2004).
Hao, H., Allen, D. L. & Hardin, P. E. A circadian enhancer mediates PER-dependent mRNA cycling in Drosophila melanogaster. Mol Cell Biol 17, 3687–3693 (1997).
So, W. V. et al. takeout, a novel Drosophila gene under circadian clock transcriptional regulation. Mol Cell Biol 20, 6935–6944 (2000).
Stanewsky, R., Jamison, C. F., Plautz, J. D., Kay, S. A. & Hall, J. C. Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila. EMBO J 16, 5006–5018 (1997).
Menet, J. S., Abruzzi, K. C., Desrochers, J., Rodriguez, J. & Rosbash, M. Dynamic PER repression mechanisms in the Drosophila circadian clock: from on-DNA to off-DNA. Genes Dev 24, 358–367 (2010).
So, W. V. & Rosbash, M. Post-transcriptional regulation contributes to Drosophila clock gene mRNA cycling. EMBO J 16, 7146–7155 (1997).
Picot, M., Klarsfeld, A., Chelot, E., Malpel, S. & Rouyer, F. A role for blind DN2 clock neurons in temperature entrainment of the Drosophila larval brain. J Neurosci 29, 8312–8320 (2009).
Shafer, O. T., Rosbash, M. & Truman, J. W. Sequential nuclear accumulation of the clock proteins period and timeless in the pacemaker neurons of Drosophila melanogaster. J Neurosci 22, 5946–5954 (2002).
Roberts, L. et al. Light evokes rapid circadian network oscillator desynchrony followed by gradual phase retuning of synchrony. Curr Biol 25, 858–867 (2015).
Sehadova, H. et al. Temperature entrainment of Drosophila’s circadian clock involves the gene nocte and signaling from peripheral sensory tissues to the brain. Neuron 64, 251–266 (2009).
Sellix, M. T., Currie, J., Menaker, M. & Wijnen, H. Fluorescence/Luminescence Circadian Imaging of Complex Tissues at Single-Cell Resolution. J Biol Rhythm 25, 228–232 (2010).
Keene, A. C. & Sprecher, S. G. Seeing the light: photobehavior in fruit fly larvae. Trends in Neurosciences 35, 104–110 (2012).
Choi, C. et al. Autoreceptor control of peptide/neurotransmitter corelease from PDF neurons determines allocation of circadian activity in drosophila. Cell Rep 2, 332–344 (2012).
Duvall, L. B. & Taghert, P. H. The circadian neuropeptide PDF signals preferentially through a specific adenylate cyclase isoform AC3 in M pacemakers of Drosophila. PLoS Biol 10, e1001337 (2012).
Shafer, O. T. et al. Widespread receptivity to neuropeptide PDF throughout the neuronal circadian clock network of Drosophila revealed by real-time cyclic AMP imaging. Neuron 58, 223–237 (2008).
Vecsey, C. G., Pirez, N. & Griffith, L. C. The Drosophila neuropeptides PDF and sNPF have opposing electrophysiological and molecular effects on central neurons. J Neurophysiol 111, 1033–1045 (2014).
Wei, H. et al. Signaling of pigment-dispersing factor (PDF) in the Madeira cockroach Rhyparobia maderae. PLoS One 9, e108757 (2014).
Klose, M. et al. Functional PDF Signaling in the Drosophila Circadian Neural Circuit Is Gated by Ral A-Dependent Modulation. Neuron 90, 781–794 (2016).
Rothermel, J. D. & Parker Botelho, L. H. A mechanistic and kinetic analysis of the interactions of the diastereoisomers of adenosine 3′, 5′-(cyclic)phosphorothioate with purified cyclic AMP-dependent protein kinase. Biochem J 251, 757–762 (1988).
Peng, I. F. et al. Temperature-dependent developmental plasticity of Drosophila neurons: cell-autonomous roles of membrane excitability, Ca2+ influx, and cAMP signaling. J Neurosci 27, 12611–12622 (2007).
Raman, I. M., Gustafson, A. E. & Padgett, D. Ionic currents and spontaneous firing in neurons isolated from the cerebellar nuclei. J Neurosci 20, 9004–9016 (2000).
Saad, Y. et al. Fly neurons in culture: a model for neural development and pathology. J Mol Histol 43, 421–430 (2012).
Flavell, S. W. & Greenberg, M. E. Signaling mechanisms linking neuronal activity to gene expression and plasticity of the nervous system. Annu Rev Neurosci 31, 563–590 (2008).
Agrawal, T., Sadaf, S. & Hasan, G. A Genetic RNAi Screen for IP3/Ca2+ Coupled GPCRs in Drosophila Identifies the PdfR as a Regulator of Insect Flight. Plos Genet 9 (2013).
Park, J. H. et al. Differential regulation of circadian pacemaker output by separate clock genes in Drosophila. Proc Natl Acad Sci USA 97, 3608–3613 (2000).
Cao, G. et al. Genetically targeted optical electrophysiology in intact neural circuits. Cell 154, 904–913 (2013).
Flourakis, M. et al. A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability. Cell 162, 836–848 (2015).
Sheeba, V., Gu, H., Sharma, V. K., O’Dowd, D. K. & Holmes, T. C. Circadian- and light-dependent regulation of resting membrane potential and spontaneous action potential firing of Drosophila circadian pacemaker neurons. J Neurophysiol 99, 976–988 (2008).
Fogle, K. J., Parson, K. G., Dahm, N. A. & Holmes, T. C. Cryptochrome is a blue-light sensor that regulates neuronal firing rate. Science 331, 1409–1413 (2011).
Ananthasubramaniam, B., Herzog, E. D. & Herzel, H. Timing of neuropeptide coupling determines synchrony and entrainment in the mammalian circadian clock. PLoS Comput Biol 10, e1003565 (2014).
Im, S. H. & Taghert, P. H. PDF receptor expression reveals direct interactions between circadian oscillators in Drosophila. J Comp Neurol 518, 1925–1945 (2010).
Ko, C. H. et al. Emergence of noise-induced oscillations in the central circadian pacemaker. PLoS Biol 8, e1000513 (2010).
Mezan, S., Feuz, J. D., Deplancke, B. & Kadener, S. PDF Signaling Is an Integral Part of the Drosophila Circadian Molecular Oscillator. Cell Rep 17, 708–719 (2016).
Seelig, J. D. et al. Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior. Nat Methods 7, 535–540 (2010).
Gummadova, J. O., Coutts, G. A. & Glossop, N. R. Analysis of the Drosophila Clock promoter reveals heterogeneity in expression between subgroups of central oscillator cells and identifies a novel enhancer region. J Biol Rhythms 24, 353–367 (2009).
Blanchardon, E. et al. Defining the role of Drosophila lateral neurons in the control of circadian rhythms in motor activity and eclosion by targeted genetic ablation and PERIOD protein overexpression. Eur J Neurosci 13, 871–888 (2001).
Hyun, S. et al. Drosophila GPCR Han is a receptor for the circadian clock neuropeptide PDF. Neuron 48, 267–278 (2005).
Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451–461 (1999).
Jiang, S. A., Campusano, J. M., Su, H. & O’Dowd, D. K. Drosophila mushroom body Kenyon cells generate spontaneous calcium transients mediated by PLTX-sensitive calcium channels. J Neurophysiol 94, 491–500 (2005).
Kuppers-Munther, B. et al. A new culturing strategy optimises Drosophila primary cell cultures for structural and functional analyses. Dev Biol 269, 459–478 (2004).
Forer, A. & Pickett-Heaps, J. Fibrin clots keep non-adhering living cells in place on glass for perfusion or fixation. Cell Biol Int 29, 721–730 (2005).
Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat Methods 9, 676–682 (2012).
Dowse, H. B. Maximum entropy spectral analysis for circadian rhythms: theory, history and practice. J Circadian Rhythms 11, 6 (2013).
We thank N.R. Glossop and the Bloomington Drosophila Stock Center for fly stocks and L. Luo and R. Tsien for plasmids. We thank C. Helfrich-Förster, S. Kadener and U. Schibler for helpful comments on the manuscript. This work was supported by the JST PRESTO program, the Swiss National Science Foundation (31003A_149893), the European Research Council (ERC-StG-311194), the Novartis Foundation for Medical-Biomedical Research (13A39) and the University of Geneva.
The authors declare no competing financial interests.
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Sabado, V., Vienne, L., Nunes, J. et al. Fluorescence circadian imaging reveals a PDF-dependent transcriptional regulation of the Drosophila molecular clock. Sci Rep 7, 41560 (2017). https://doi.org/10.1038/srep41560
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.