Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Thumb Imprint Based Detection of Hyperbilirubinemia Using Luminescent Gold Nanoclusters


Early and easy detection of diseases, using point-of-care and inexpensive devices, not only provides option for early treatment but also reduces the risk of propagation. Herein we report the fabrication of a robust film based luminescence indicator of bilirubin, which can indicate hyperbilirubinemia through the thumb imprint of the patient. The UV-light induced luminescence intensity of the film, made out of chitosan stabilised gold (Au) nanoclusters, which was effectively quenched in the presence of Cu2+ ions, recovered in the presence of bilirubin from skin or blood serum. Moreover, the sensitivity of detection of bilirubin was tuneable with the amount of Cu2+ added, thereby facilitating the detection of the desired concentration range of bilirubin.


The significant increase in global population - in a rather short period of time - demand addressing persistent healthcare issues of a large populace. The current stress on the development of point-of-care diagnostics, especially important for remote and resource-deficient regions, is engendering the development of easy to use devices1. Among the widely occurring disease conditions, hyperbilirubinemia, which is prevalent among the newborns and jaundice afflicted patients, is a health condition where bilirubin concentration in blood plasma crosses 1.19 × 10−2 mg/mL in adults and 19.8 mg/mL in the newborns. Bilirubin, produced from the catabolism of hemoglobin present in the red blood cells, is excreted through bile and urine. The disease is generally correlated with liver disfunction or biliary obstruction; however, hemolytic anemia can also lead to production of excess bilirubin. It is also associated with jaundice leading to yellowing of skin color. Typically, hyperbilirubinemia is identified in the sclera (protective outer layer of the eye) by visual observation, urine, stool and other mucous membranes with the appearance of yellow color2. Early detection and treatment hold the keys to preventing the condition reaching to critical level. These call for the use of the latest of science and technology for the development of the smartest of devices useful for common people at large.

In this regard, nanoscale science and technology - in combination with chemistry-based technology - provide ingenious options in probing health conditions at the highest possible sensitivity and at the fastest speed. For example, conventional pathological laboratory tests use biochemical reactions to provide information - among a plethora of other molecular markers - about total sugar, cholesterol, triglycerides and uric acid. These are generally based on optical markers with absorption in the visible region of wavelengths being the primary indicator. Moreover, research efforts over the past decades have led to significant advancements in disease detection using microRNAs as biomarkers. With regard to cancer detection, microRNA based markers have been employed for accurate prognosis of malignancy3. Further, “amplification free” counting of microRNAs has been made possible by a kinetic fingerprint based approach, which surpasses conventional techniques in terms of specificity and identifying false positive results4. Recent reports also lay stress on developing biosensors based on principle of “mechanochemical” sensing of analytes, which allow sensitive detection at single molecule level5. On the other hand, nanoscale particles such as metal nanoparticles, quantum dots, carbon dots, graphene quantum dots and atomic clusters usually provide higher sensitivity to detection using either surface plasmon resonance based optical changes or changes in the emission characteristics of the nanoparticle based sensor6,7,8. Thus, through observation of changes in their characteristics optical properties, identification of specific DNA and RNA sequences, proteins, toxins, bacteria and viruses have been possible9,10,11. In contrast, luminescent nanomaterials mentioned above offer sensitive detection of pathogens, important biomolecules and narcotics and act as molecular markers of DNA and protein based diseases12,13. The large Stokes-shifted and tunable emission of the materials provide additional advantage of sensing molecules even at the femto-molar scale. As regards to hyperbilirubinemia, a recent report involving luminescence quenching in the presence of bilirubin indicated potential for use of Au nanoclusters for such sensing14. However, selectivity, specificity and potential device fabrication, which were not addressed in the report, are essential for the development of a practicable sensor. This is where the principle of chemistry could be employed in conjunction with the versatility of nanomaterials.

Herein we report the fabrication of a new nanotechnology-based luminescent sensor for hyperbilirubinemia. The device is a film made of chitosan-stabilized Au nanoclusters embedded in a polyvinylidene difluoride (PVDF) membrane. The said device was capable of detecting bilirubin with high sensitivity through thumb impression or from the blood plasma of a jaundice afflicted patient. In addition, we report a luminescence based detection of blood bilirubin with high sensitivity in the liquid phase. The film appeared luminescent yellow in the presence of UV light. The luminescence intensity was quenched in the presence of copper salt such as copper sulphate. On the other hand, in the presence of bilirubin, the intensity was restored.

Results and Discussions

Experimentally, luminescent Au nanoclusters in chitosan were synthesized by modifying a protocol established in our laboratory15 (Supplementary Fig. S1A,B). Gradual addition of copper sulphate solution (12.5 mg/mL) to the 0.8 mL aqueous dispersion of the clusters (0.1 mL Au nanoclusters dispersion and 0.7 mL glycine buffer) led to lowering of intensity of luminescence16 (Fig. 1A). The pH of the medium was maintained at 2.5 using glycine buffer (Supplementary Information), which was required for subsequent experiment as bilirubin is known to quench the luminescence of Au nanoclusters at pH above 2.59 (Supplementary Fig. S2). Corresponding Stern-Volmer constant was calculated to be (1.65 ± 0.21) × 10−3 M−1 (Fig. 1B). It was further observed that addition of bilirubin to a system comprising of Cu2+ added Au nanoclusters (the luminescence of which was quenched due to the presence of Cu2+ ions) led to the recovery of the luminescence. Thus when a solution containing 1.38 mg/mL of copper ions in 0.8 mL Au nanocluster dispersion was subsequently treated with increasing amount of bilirubin, the intensity of luminescence increased systematically up to full recovery (Fig. 1C). Control experiment involving addition of water (instead of bilirubin solution) did not lead to significant change in the luminescence intensity (Supplementary Fig. S3). Further bilirubin did not seem to have any effect on luminescence intensity of the clusters in the absence of Cu2+ions (Supplementary Fig. S4A,B). Also, a mixture of Cu2+(12.10 mg/mL) and bilirubin (5 × 10−4 mg/mL), when added to Au nanocluster dispersion, did not lead to significant change in luminescence intensity when compared to the effect of sequential addition of copper ions and then bilirubin (Supplementary Fig. S5). The results suggested important role of bilirubin in the restoration of Au nanocluster luminescence even in the presence of Cu2+ ions. Similar experiments were performed with different concentrations of copper ions and bilirubin (Fig. 2A). Corresponding plots of recovery of luminescence versus concentrations of adducts (akin to Stern-Volmer plot) were obtained (Fig. 2B). The purpose of the experiments was to find the lowest concentration of bilirubin, which would ensure the observation of change of luminescence with the highest sensitivity. It was found that bilirubin concentration as low as 8.4 × 10−3 mg/mL could provide detectable change in the luminescence intensity of Au nanoclusters (through recovery) in the presence of Cu2+ ions, the concentration of which could be as high as that of 4.77 mg/mL. On a similar note, blood serum of a jaundice afflicted patient with bilirubin concentration of 6.4 × 10−2 mg/mL was diluted a thousand times and added to Au nanoclusters dispersion with lower Cu2+ concentration (1.38 × 10−2 mg/mL). This also resulted in significant luminescence recovery. Thus, it was possible to detect varying amount of bilirubin just by tuning the amount of Cu2+ ions (Supplementary Table S1). The recovery of luminescence intensity of the nanoclusters by bilirubin in the presence of 4.77 mg/mL Cu2+ ions could be fitted with single exponential function. The results showed that use of higher concentration of Cu2+ ions in the medium helped detect higher amounts of bilirubin.

Figure 1
figure 1

(A) Gradual quenching of luminescence intensity of Au nanoclusters upon gradual addition of Cu2+. Photoluminescence emission (PLE) spectrum of (a) Au nanoclusters and of that following addition of (b) 3.058 × 10−1 mg/mL, (c) 5.971 × 10−1 mg/mL, (d) 8.748 × 10−1 mg/mL, (e) 1.14 mg/mL, (f) 1.393 mg/mL, (g) 1.635 mg/mL, (h) 1.867 mg/mL, (i) 2.090 mg/mL, (j) 2.303 mg/mL, (k) 2.505 mg/mL and (l) 2.704 mg/mL Cu2+ salt solution. (B) Corresponding Stern-Volmer plot. I0 is the luminescence intensity of as-synthesized Au nanoclusters and I is the reduced luminescence intensity of the same on subsequent addition of Cu2+ ions. (C) The figure shows luminescence intensity quenching of Au nanoclusters upon addition of Cu2+ and recovery of the same on addition of BR (bilirubin). PLE spectrum of (a) Au nanoclusters and of that following addition of (b) 1.38 mg/mL Cu2+ and then (c) 5.8 × 10−4 mg/mL BR, (d) 1.1 × 10−3 mg/mL BR and (e) 1.5 × 10−3 mg/mL BR, respectively. BR means bilirubin.

Figure 2
figure 2

(A) Graphs showing gradual luminescence intensity recovery of Au nanoclusters otherwise quenched in the presence of Cu2+ ions. PLE spectrum of (a) Au nanocluster dispersion and of that following addition of (b) 4.77 mg/mL Cu2+, (c) 2.1 × 10−3 mg/mL BR, (d) 3.8 × 10−3 mg/mL, (e) 5.2 × 10−3 mg/mL BR, (f) 6.5 × 10−3 mg/mL BR, (g) 7.5 × 10−3 mg/mL BR and (h) 8.4 × 10−3 mg/mL BR, respectively. (B) Gradual increase of normalized luminescence intensity of the Cu2+ quenched Au nanocluster dispersion after addition of bilirubin. I0 is the reduced luminescence intensity of the nanoclusters in the presence of copper ions. I is the recovered luminescence intensity on subsequent addition of BR. BR means bilirubin.

Time-resolved photoluminescence measurements indicated average luminescence lifetime of as-synthesized Au nanoclusters to be 1.56 μs, which decreased to 1.29 μs upon addition of 0.2 mL of Cu2+ (5 × 10−4 M) and subsequently was restored to 1.53 μs upon further addition of bilirubin (from blood serum with bilirubin concentration of 6.4 × 10−5 mg/mL) (Supplementary Fig. S6). The quenching of luminescence of Au nanoclusters by Cu2+ ions is likely to be dynamic in nature. This is apparent from the fact that in addition to the linear nature of the Stern-Volmer plot for quenching of luminescence of Au nanoclusters by Cu2+ ions, significant changes in the luminescence lifetime of the nanoclusters in the presence of Cu2+ was observed. Interestingly, UV-vis spectrum indicated the appearance of a peak at 350 nm owing to the Cu-bilirubin complex17,18, which was neither present in the sample containing Au nanoclusters nor in Cu2+ ion added Au nanoclusters (Supplementary Fig. S7). Thus, overall, while Cu2+ addition led to quenching of luminescence due to Au nanoclusters, addition of bilirubin led to recovery of the same, possibly owing to the formation of Cu-bilirubin complex17,18.

Since the luminescence of the Au nanoclusters (embedded in chitosan) was responsive to the presence of Cu2+ ions and subsequently to bilirubin an opportunity was apparent with respect to making a device for the detection of bilirubin with high sensitivity. Also since bilirubin is known to form complex with Cu2+ having known stoichiometry17, the detection could have the advantage of being quantitative. Further, common techniques which involve loss or lowering of luminescence in the presence of an analyte may not necessarily lead to detection with high sensitivity. On the other hand, recovery of luminescence in the presence of an analyte may offer unique opportunity of sensing with enhanced sensitivity. This – in principle – could provide an eminent opportunity for making a solid-state device for the above purpose.

In order to pursue the above objective, the as-prepared Au nanoclusters were drop-cast on a PVDF membrane (which was cut in the form of a film of ~2.8 × 2.8 cm2 size) in which the bright yellow-orange luminescence of the clusters in the presence of UV light (254 nm) could easily be observed (Fig. 3A). Further, when the film was treated with CuSO4 solution (0.2 mL of 12.5 mg/mL) the luminescence disappeared nearly completely in the area where the liquid was dropped (Fig. 3B). On the other hand, when the same film was further treated with 0.2 mL of 1.1 × 10−2 mg/mL bilirubin the luminescence recovered with great clarity (Fig. 3C). Control experiment, involving addition of water to the copper treated (and Au nanoclusters coated) PVDF membrane did not show restoration of luminescence intensity, thus clearly indicating the role of bilirubin in the recovery (Supplementary Fig. S8). It is plausible that Cu2+ ions when present in the film (following addition of an aqueous solution of CuSO4) acted as the quencher of luminescence. However, when bilirubin solution was added to the film, Cu-bilirubin complex formation might have taken place. The complex might not have quenched the luminescence and thus the recovery of luminescence was achieved. The above results indicated the potency of a new device in the form of solid film for the detection of bilirubin present in the aqueous medium.

Figure 3
figure 3

Photograph of (A) Au nanocluster coated polyvinylidene difluoride (PVDF) membrane (with dimension of the films being 2.8 × 2.8 cm2) as visualized under UV lamp with excitation at 254 nm. (B) Au nanocluster coated PVDF membrane as observed using 254 nm UV light, following addition of copper sulphate solution. The luminescence can be observed to have been reduced substantially. (C) BR (bilirubin) treated film as viewed using 254 nm UV light. The restoration of the intense yellow-orange color indicated recovery of luminescence of the film.

As discussed above, bilirubin is known to be present in the skin of a person affected with hyperbilirubinemia. The fact that deposition of bilirubin occurs on skin of a jaundice afflicted patient, has been widely used as a tool to correlate serum bilirubin concentration. In this regard, various studies, including detailed theoretical study, involving mechanism of transfer of bilirubin from plasma to skin have been reported19,20,21. Thus, probing the molecular signature of a disease – for example hyperbilirubinemia in the present study- through chemical reaction with another species, thereby leading to prominent optical changes, could emerge as a new avenue for disease diagnosis. Hence it was deemed feasible to test the efficacy of the device in detecting the presence of excess bilirubin through the thumb imprint of an affected patient. In order to test this a film (as above) was fabricated consisting of Au nanoclusters (embedded in chitosan) in PDVF membrane. The luminescence of the film was recorded using UV light as the excitation source (Fig. 4A). The film was then treated with 0.02 mL of 12.5 mg/mL CuSO4 solution upon which the luminescence disappeared (Fig. 4B). Interestingly, when the thumb of a patient afflicted with hyperbilirubinemia was pressed against the copper-treated film for 5 min, the recovery of luminescence of the film could be observed (Fig. 4C). Pathological test in the laboratory indicated the concentration of bilirubin in the patient’s blood sample to be 3.1 × 10−2 mg/mL, which was well above the limit of concentration of a healthy adult. This experimental result has been presented thrice to support the repeatability of the results. In an analogous set of experiment, involving thumb imprint of a jaundice afflicted patient with bilirubin level recorded as 4.1 × 10−2 mg/mL, the quenched luminescence intensity of copper treated Au nanocluster film showed restoration of luminescence intensity when thumb imprint of the said patient was acquired on the film (Supplementary Fig. S9). On the other hand, when the same patient, immediately following the first impression, pressed the same thumb on another (similar) film of Au nanoclusters added with Cu2+ ions, no significant fluorescence recovery was obtained (Supplementary Fig. S10A–C). This could be due to insufficient bilirubin deposition on the patient’s skin following the first impression. Control experiment with healthy volunteer did not lead to any recovery of the luminescence of the copper-treated film (Supplementary Fig. S11). Similar results (for patients with bilirubin level within and above normal range) were obtained when chitosan stabilized Au nanoclusters were dried as film instead of coating on to PVDF membrane (Supplementary Fig. S12). In an allied vein, detection of bilirubin in blood serum of a jaundice afflicted patient (total bilirubin count of 6.4 × 10−2 mg/mL) was also possible using the liquid phase method (Supplementary Fig. S13).

Figure 4
figure 4

(A) Digital photograph of Au nanoclusters containing PVDF membrane (dimension of the films was 2.9 × 2.1 cm2). (B) Copper salt (20 μL 12.4 mg/mL) treated Au nanoclusters containing PVDF membrane. The low luminescence region is due to quenching by Cu2+ ions added to the membrane. (C) The same film after thumb impression of a jaundice afflicted patient. The photographs were recorded following illumination with UV light (254 nm).


In essence, we have developed a new nanotechnology-based fast and easy method of identifying hyperbilirubinemia in patients afflicted with jaundice. The method invented provided an option to circumvent the need of blood test for fast analysis and used thumb impression instead for testing of hyperbilirubinemia. The method (and the device) could be considered cost-effective and versatile, as the analysis could be performed, in minutes, without the need of a typical pathological laboratory setup. The method involved the use of luminescent Au nanocluster film, the intensity of which was effectively quenched in the presence of copper salt. The detection method relied on the recovery of luminescence intensity in the presence of bilirubin. The change in intensity was equally effective in liquid medium as well as in the solid phase, thus providing a detection with high sensitivity. The facile detection of bilirubin was possible using blood serum as well as through thumb impression of the patient. The best sensitivity of detection of BR in the liquid phase was 6.4 × 10−5 mg/mL and that in the solid phase was achieved with the concentration of blood bilirubin in serum of the affected patient being ~3.1 × 10−2 mg/mL. The bilirubin detection technique developed herein is based on complexation reaction between bilirubin and copper ions and thus offers superiority in terms of selectivity over other conventional luminescence quenching based methods. However, disease markers capable of forming stronger complex with copper ions as compared to bilirubin might interfere in detection method developed herein. Also, significant alteration in pH of the system (beyond the buffer capacity of glycine buffer used herein) might lead to complexity in detection of hyperbilirubinemia by the said method as the luminescence of chitosan stabilized nanoclusters used here is itself sensitive to pH. However, for the solid device such an effect may not arise. In a nutshell, the solid device thus designed is superior to the commonly practiced method as it enabled prompt, easy and precise detection of jaundice and could be used as a point-of-care device especially by common mass in a resource-limited region.

Materials and Methods


Chitosan (Sigma Aldrich), tetra chloro auric acid (HAuCl4, Sigma Aldrich) mercaptopropionic acid (MPA, Sigma Aldrich), copper sulphate (Merck), bilirubin (BR, Alfa Aesar), glycine, sodium chloride and HCl were used as received. Milli-Q grade water was used for all experiments.

Synthesis of luminescent Au nanoclusters

Au nanoclusters were prepared by adding 1.2 mL of aqueous solution of HAuCl4 (10 mM) to 20 mL of 0.5% (w/v) chitosan (which was solubilized using 0.1% glacial acetic acid) under stirring condition. This was followed by addition of 0.8 mL mercaptopropionic acid (MPA; 0.11 M). Stirring was continued for 30 min and the pH of the resulting medium was kept at or below 2.5 using 0.01 M HCl.

Preparation of glycine buffer

58.0 mg of sodium chloride was added with 75.0 mg glycine in 10.0 mL water. The pH of the resulting medium was kept at 2.5 using 0.01 M HCl.

Preparation of Au nanocluster dispersion for luminescence experiment

To 0.1 mL of Au nanocluster dispersion prepared using the above mentioned protocol (experimental section A), 0.7 mL of glycine buffer (experimental section C) was added. This was deemed essential to keep the absorbance value of the Au nanocluster dispersion below 0.1 in order to avoid self-absorption. Also, the pH of the medium was checked to ensure a value below 2.5. To this medium, required amount of copper sulphate and bilirubin solutions were added for further experiments.

Preparation of bilirubin solution

Required amount of bilirubin was dissolved in appropriate amount of water and added with 1 μL of 1 M NaOH.

Preparation of Au nanocluster containing film

The medium (30 mL) containing as-synthesized Au nanoclusters was poured onto a petri dish (Tarsons, disposable sterile petridish) and was then allowed to dry overnight in an oven at 55 °C. A film was formed with the approximate dimension of 3 × 3 cm2. The intrinsic luminescent properties of Au nanoclusters remained intact after the formation of the film. The film had yellow-orange luminescence upon excitation with 254 nm UV light.

In a similar way, the as-prepared Au nanocluster dispersion (1.0 mL) was drop-cast on an approximately 3 × 3 cm2 polyvinylidene difluoride (PVDF) membrane and the coated membrane –following drying - was used for further experiments. Since the films have been subjected to evaporation following drop casting of gold nanoclusters, the intensity of luminescence of gold nanoclusters (upon exposure to UV light) on the periphery of the film showed higher intensity owing possibly due to “coffee-ring effect”22.

Treatment of the Au nanocluster containing film with copper sulphate

The Au nanoclusters coated polyvinylidene difluoride (PVDF) membrane (as above) was treated with 0.2 mL CuSO4 (12.5 mg/mL) solution. The film was then dried in air for 30 min and was observed under UV light. The luminescence intensity of the film got substantially reduced (upon exposure to 254 nm UV light) following addition of the copper salt.

Interaction of bilirubin solution with copper sulphate added Au nanocluster containing film

0.2 mL aqueous solution of bilirubin (1.1 × 10−2 mg/mL) was added to Au nanocluster containing film, which was previously treated with 0.2 mL of CuSO4 (12.5 mg/mL) solution. The film was then dried for 30 min and then was viewed using UV light. Recovery of the yellow-orange luminescence of the film was observed.

Interaction of copper salt with luminescent Au nanoclusters in liquid phase

To the above prepared dispersion of Au nanoclusters (experimental section E), varying concentration of copper sulphate solution was added sequentially for various experiments. The concentration for each of the solutions of copper sulphate is mentioned in the respective figure legends.

Interaction of bilirubin with copper salt added luminescent Au nanoclusters in liquid phase

To Au nanocluster dispersion added with different amounts of copper salt, varying amount of bilirubin (both laboratory chemical and present in the blood serum) was added for various experiments, the concentration for each of which is mentioned in the figure legends.

Control experiments

Control experiments including addition of water of equal amount as that of bilirubin solution to copper sulphate added Au nanoclusters, addition of bilirubin to pristine Au nanoclusters, addition of a mixture of copper-bilirubin mixture to Au nanoclusters, effect of thumb imprint of a volunteer with bilirubin concentration in normal range on copper salt treated film of Au nanoclusters, effect of thumb imprint of a jaundice afflicted patient on a film (copper treated Au nanoclusters) immediately following imprint on a similar film were performed.

Collection of serum and thumb imprint of patients affected with hyperbilirubinemia

Serum and thumb imprints of jaundice patients were collected from hospital affiliated to Indian Institute of Technology Guwahati, India with full consent of the volunteers, following institutional protocol. Additional imprints were obtained from Guwahati Neurological Research Centre (GNRC) hospital, North Guwahati, Guwahati following appropriate procedure.

Additional Information

How to cite this article: Basu, S. et al. Thumb Imprint Based Detection of Hyperbilirubinemia Using Luminescent Gold Nanoclusters. Sci. Rep. 6, 39005; doi: 10.1038/srep39005 (2016).

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.


  • St John, A. & Price, C. P. Existing and Emerging Technologies for Point-of-Care Testing. Clin Biochem Rev. 35, 155–167 (2014).

    PubMed  PubMed Central  Google Scholar 

  • Maisels, M. J. Managing the jaundiced newborn: a persistent challenge. CMAJ. 187, 335–343 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  • Mitchell, P. S. et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci. 105, 10513–10518 (2008).

    ADS  CAS  Article  PubMed  Google Scholar 

  • Johnson-Buck, A. et al. Kinetic fingerprinting to identify and count single nucleic acids. Nat Biotech. 33, 730–732 (2015).

    CAS  Article  Google Scholar 

  • Shrestha, P., Mandal, S. & Mao, H. Mechanochemical Sensing: A Biomimetic Sensing Strategy. ChemPhysChem 16, 1829–1837 (2015).

    CAS  Article  PubMed  Google Scholar 

  • Radhakumary, C. & Sreenivasan, K. Naked Eye Detection of Glucose in Urine Using Glucose Oxidase Immobilized Gold Nanoparticles. Anal. Chem. 83, 2829–2833 (2011).

    CAS  Article  PubMed  Google Scholar 

  • Chang, H. C. & Ho, J.-a. A. Gold Nanocluster-Assisted Fluorescent Detection for Hydrogen Peroxide and Cholesterol Based on the Inner Filter Effect of Gold Nanoparticles. Anal. Chem. 87, 10362–10367 (2015).

    CAS  Article  PubMed  Google Scholar 

  • Kumar, A. et al. A paper based microfluidic device for easy detection of uric acid using positively charged gold nanoparticles. Analyst 140, 1817–1821 (2015).

    ADS  CAS  Article  PubMed  Google Scholar 

  • Wang, R. et al. Rapid and sensitive detection of Salmonella typhimurium using aptamer-conjugated carbon dots as fluorescence probe. Anal. Methods. 6, 6802–6808 (2014).

    CAS  Article  Google Scholar 

  • Zhang, C.-Y., Yeh, H.-C., Kuroki M. T. & Wang, T.-H. Single-quantum-dot-based DNA nanosensor. Nat Mater. 4, 826–831 (2005).

    ADS  CAS  Article  PubMed  Google Scholar 

  • Wu, F. et al. Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay. Biosens. Bioelectron. 77, 464–470 (2016).

    CAS  Article  PubMed  Google Scholar 

  • Chinen, A. B. et al. Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence. Chem. Rev. 115, 10530–10574 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Sun, J. & Yang, X. Gold nanoclusters–Cu2+ ensemble-based fluorescence turn-on and real-time assay for acetylcholinesterase activity and inhibitor screening. Biosens. Bioelectron. 74, 177–182 (2015).

    CAS  Article  PubMed  Google Scholar 

  • Santhosh, M., Chinnadayyala, S. R., Kakoti, A. & Goswami, P. Selective and sensitive detection of free bilirubin in blood serum using human serum albumin stabilized gold nanoclusters as fluorometric and colorimetric probe. Biosens. Bioelectron. 59, 370–376 (2014).

    CAS  Article  PubMed  Google Scholar 

  • Sahoo, A. K., Banerjee, S., Ghosh, S. S. & Chattopadhyay, A. Simultaneous RGB Emitting Au Nanoclusters in Chitosan Nanoparticles for Anticancer Gene Theranostics. ACS Appl. Mater. Interfaces. 6, 712–724 (2014).

    CAS  Article  PubMed  Google Scholar 

  • Sailapu, S. K., Sahoo, A. K., Ghosh, S. S. & Chattopadhyay, A. Hierarchical Logic Structures Based on Responsive Fluorescent Gold Nanoclusters. Small 10, 4067–4071 (2014).

    CAS  Article  PubMed  Google Scholar 

  • Adhikari, S., Joshi, R. & Gopinathan, C. Bilirubin as an anti precipitant against copper mediated denaturation of bovine serum albumin: formation of copper-bilirubin complex. Biochimica et Biophysica Acta. 1380, 109–114 (1998).

    CAS  Article  PubMed  Google Scholar 

  • Stocker, R. & Ames, B. N. Potential role of conjugated bilirubin and copper in the metabolism of lipid peroxides in bile. Proc Natl Acad Sci. 84, 8130–8134 (1987).

    ADS  CAS  Article  PubMed  Google Scholar 

  • Knudsen, A. The cephalocaudal progression of jaundice in newborns in relation to the transfer of bilirubin from plasma to skin. Early Hum Dev. 22, 23–28 (1990).

    CAS  Article  PubMed  Google Scholar 

  • Knudsen, A., Ebbesen, F., Hansen H. & Brodersen R. The increase of yellow skin colour beyond that of serum bilirubin: A proposed indicator of risk for bilirubin encephalopathy in the newborn. Acta Paediatrica Japonica. 35, 418–422 (1993).

    CAS  Article  PubMed  Google Scholar 

  • Knudsen, A. & R., Brodersen . Skin color and bilirubin in neonates Arch. Dis. Child. 64, 605–609 (1989).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  • Deegan, R. D. et al. Capillary flow as the cause of ring stains from dried liquid drops Nature 389, 827–829 (1997).

    ADS  CAS  Article  Google Scholar 

Download references


We thank the Department of Electronics and Information Technology, Government of India for fund (No. 5(9)/2012-NANO (Vol. II)). Assistances from CIF (IIT Guwahati), Dr. Padmaja Prasad Mishra, Bandhan Chatterjee, Subhas Chandra Bera, IIT Guwahati hospital, Dr. Madhumita Das and GNRC hospital are acknowledged.

Author information

Authors and Affiliations



A.C. conceived the idea, helped in implementation of experiments, contributed further ideas and wrote the manuscript. A.P. contributed ideas and helped in implementation of experiments. A.K.S. contributed ideas. S.B. executed the idea, performed experiments and wrote the manuscript.

Ethics declarations

Competing interests

An Indian patent has been filed and published based on this work and filing of international patent is under process.

Electronic supplementary material

Rights and permissions

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Basu, S., Sahoo, A., Paul, A. et al. Thumb Imprint Based Detection of Hyperbilirubinemia Using Luminescent Gold Nanoclusters. Sci Rep 6, 39005 (2016).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing