Metaldehyde is extensively used worldwide as a contact and systemic molluscicide for controlling slugs and snails in a wide range of agricultural and horticultural crops. Contamination of surface waters due to run-off, coupled with its moderate solubility in water, has led to increased concentration of the pesticide in the environment. In this study, for the first time, rapid analysis (<~1 minute) of metaldehyde residues in water is demonstrated using paper spray mass spectrometry (PS-MS). The observed precursor molecular ions of metaldehyde were confirmed from tandem mass spectrometry (MS/MS) experiments by studying the fragmentation patterns produced via collision-induced dissociation. The signal intensity ratios of the most abundant MS/MS transitions for metaldehyde (177 → 149 for protonated ion) and atrazine (221 → 179) were found to be linear in the range 0.01 to 5 ng/mL. Metaldehyde residues were detectable in environmental water samples at low concentration (LOD < 0.1 ng/mL using reactive PS-MS), with a relative standard deviation <10% and an R2 value >0.99, without any pre-concentration/separation steps. This result is of particular importance for environmental monitoring and water quality analysis providing a potential means of rapid screening to ensure safe drinking water.
Detection and quantification of contaminants or pollutants in surface waters is of great importance to ensure safety of drinking water and for the aquatic environment1,2,3,4,5,6. Metaldehyde (CH3CHO)4 is a cyclic tetramer of acetaldehyde and is used extensively around the world as a molluscicide in agriculture for the control of slugs to protect crops. Large amounts of metaldehyde residues (from ‘slug pellets’) become mobilized, especially during periods of rainfall, seeping into reservoirs, rivers and groundwater, from which drinking water is sourced. Although metaldehyde has low toxicity, cases of metaldehyde poisoning and death in both humans and animals have been reported6,7,8. The United States Environmental Protection Agency (EPA) re-registered metaldehyde as a ‘restricted use pesticide’ and required risk-reduction measures to be adopted due to the potential short-term and long-term effects on wildelife9,10. The World Health Organization (WHO) classifies metaldehyde as a “moderately hazardous” pesticide (class II)11. In Europe, the European Commission has adopted a directive that restricts pesticides levels to 0.1 μg/L in drinking water12,13. Water companies and environmental agencies are under increasing pressure to routinely monitor levels of metaldehyde residues in water courses as part of their legal obligation14. As such there is an increasing need to develop effective analytical methods for detecting and quantifying metaldehyde in water samples at the source. In particular in-situ monitoring is required to ensure water management practices are based on empirical, up-to-date information which provides a better understanding of competing factors, risk and requirement.
Rapid analytical methods for in-situ analysis of metaldehyde in water, if available, would provide critical information on water quality for water companies and regulation bodies to manage exposures. Quantitative analysis of metaldehyde has been reported using various ex-situ methods based on solid-phase extraction8,15 followed by gas chromatography (GC) or high performance liquid chromatography (HPLC) with mass spectrometry (MS)7,14,15,16,17,18. However, each of these analytical methods involves extensive sample preparation including extraction, separation, and derivatization, resulting in increased cost and time of analysis. As will be demonstrated in this study, ambient ionization (AI) combined with tandem mass spectrometry (MS/MS) can overcome such limitations19,20,21,22.
AI is a form of ionization that is performed on unmodified samples in open air and the method is capable of providing almost instantaneous data while minimizing sample preparation22,23,24,25,26,27,28,29. Some of the most popular AI techniques include desorption electrospray ionization (DESI)30, extractive electrospray ionization (EESI)31,32,33,34,35,36, desorption atmospheric pressure chemical ionization (DAPCI)37,38,39, and direct analysis in real time (DART)40,41. AI-MS shows promise as an analytical tool for in-situ applications and has been demonstrated in a variety of fields where timely intervention is highly desirable such as: homeland security23, food safety42, pharmaceutical drug development43, and environmental monitoring44. There are several advantages to using in-situ AI methods capable of onsite analysis. The foremost advantage is the provision of data in real-time (or near real-time) at the point of interest allowing key management decisions to be taken in a timely manner. Subsidiary advantages relate to the chain of custody: by effectively taking the lab to the sample rather than the sample to the lab, the sample integrity is maintained and sampling/handling costs are significantly reduced.
The objective of the present study is to develop a new method for rapid detection and quantitative analysis of metaldehyde using AI-MS, based on paper spray (PS) ionization. PS-MS is a relatively new AI technique, first reported by Cooks, Ouyang & co-workers45 in 2010. PS has since been demonstrated for the analysis of a wide range of samples including bio-fluids46,47,48, bio-tissues49, protein complexes50, foodstuffs51,52,53, beverages54,55, bacteria56 and biocides57. The technique has undergone various developments such as, high throughput implementation58, application if carbon nanotube impregnated paper enabling low voltage application59, integration with solid phase extraction60 and printing hydrophobic wax barriers on to the paper substrate for extended solvent supply61. According to the authors’ knowledge, this study marks the first time that PS-MS has been utilized for the analysis of metaldehyde in water. The use of paper as a substrate material in analytical chemistry has been demonstrated for several decades and has many advantages such as: it has high surface area-to-volume ratio, it is readily available at low-cost, it wicks aqueous fluids, it is biodegradable and lightweight allowing for easy transportation and storage. In a typical PS experiment, a cellulose chromatographic paper is cut into equilateral triangles with ~5 mm sides using scissors and is wetted with a solvent. Charged droplets are emitted from the paper tip when a high DC voltage (±3–5 kV) is applied. Droplet emission occurs presumably via Taylor cone formation, which leads to analyte(s) ionization through electrospray like (and/or other unidentified) mechanisms62. Moreover, analysis by PS-MS requires little or no sample preparation and the entire full MS or MS/MS experiment can be completed within seconds (<1 minute). In comparison to other ambient ionization methods, PS integrates three analytical procedures: sample collection, separation, and ionization into a single experimental step making it more attractive for rapid and direct analysis of analyte(s) in complex mixtures. In addition, no nebulizer gases are required so the technique can be more readily used with portable MS in the field.
In the present study, experiments were carried out using a commercial benchtop ion trap mass spectrometer coupled with PS ionization (Fig. 1). Sample preparation was reduced to dissolving the model compounds (metaldehyde and paraldehyde) in methanol/water to form a stock solution (1000 ppm), that was serially diluted with water to the desired concentration before analysis, while raw environmental water samples (Abberton Raw & Chigwell Raw) were analyzed directly as supplied (from Northumbrian Water, UK) without any dilution. The results show that <0.1 ng mL−1 of metaldehyde in environmental water placed onto paper can be detected using a commercial benchtop mass spectrometer. The limit of detection (LOD) obtained was 0.05 ng mL−1 and below the permitted minimum EU levels for drinking water; good linearity (R2 = 0.9986) and accuracy (relative standard deviation ~7%) were also achieved. We further characterized the analyte(s) identity by analyzing the fragmentation patterns of metaldehyde in water using tandem mass spectrometry (MS/MS). We identified that the cyclic nature of metaldehyde can encourage the inclusion of different ions (H+, Na+ and NH4+) to enable the formation of corresponding metaldehyde ion types when analyzed using appropriate spray solvents. This capability was assessed in reactive paper spray experiments, offering more than an order of magnitude enhancement in detection limits. When collisionally activated, each ion type ([M + H]+, [M + Na]+, and [M + NH4]+) dissociated through unique pathways leading to the generation of distinctive product ions. These fragmentation patterns were fully characterized through MS/MS experiments.
Analysis of metaldehyde using PS-MS
In this study we report the direct detection of residues of metaldehyde in water using PS-MS. Figure 2 shows the mass spectra of metaldehyde (MW 176) obtained in positive ion mode using paper spray ionization with methanol as the spray solvent. A dominant sodium adduct ion [M + Na]+ of metaldehyde at m/z 199 and a less intense ammonium adduct ion [M + NH4]+ at m/z 194 were observed (Fig. 2a). Insert (i) in Fig. 2a shows the isotopic distribution of the metaldehyde sodiated adduct [M + Na]+ at m/z 199. To confirm the identity of the molecular sodiated ion [M + Na]+ attributed to m/z 199, product ion MS/MS spectra were recorded using collision-induced dissociation (CID). The result from this experiment is shown in insert (iii), Fig. 2a, which indicates that, upon CID activation, the ion at m/z 199 yields a predominant fragment ion at m/z 67. This ion corresponds to sodiated acetaldehyde (MW 44) formed from the sequential loss of neutral dimer (MW 88) and monomer (MW 44) of acetaldehyde. Indeed, the intermediate fragment ion formed after the dissociation of the acetaldehyde dimer is observed at m/z 111, followed by the elimination of the monomer. A competing fragmentation pathway to the loss of the dimeric acetaldehyde was deemed to correspond to the elimination of a water (18 Da) molecule to give a less intense fragment ion peak at m/z 181. The less intense ammoniated molecular ion peak observed at m/z 194 was also confirmed via CID (see Supplementary Figure S1, Supplementary Information). Upon CID activation, the ion at m/z 194 yields a fragment through sequential loss of two water (18 Da) molecules, yielding intense product ions at m/z 176 and m/z 158 (major).
The sodiated molecular ion and fragmentation assignments were further investigated using deuterated metaldehyde-d16 (MW 192) as a model compound sample. Here too, a dominant sodiated molecular ion [M + Na]+ at m/z 215 was observed demonstrating that adduction with the Na+ ion was unaffected by isotopic substitution (Fig. 2b). Insert (ii) in Fig. 2b shows the isotopic distribution of the metaldehyde sodiated adduct [M + Na]+ at m/z 215. These sodium adducts were formed with relatively low internal energy as indicated by the absence of associated fragmentation observed in the full mass spectrum (Fig. 2). Insert (iv), Fig. 2b, shows the CID data for the intact metaldehyde-d16 sodiated molecular [M + Na]+ ion at m/z 215, which upon collisional activation dissociates yielding a more intense fragment ion at m/z 71 [CD3CDO + Na] via sequential elimination of dimeric (96 Da) and monomeric (48 Da) acetaldehyde-d4 without H/D scrambling. The stability and abundance of the precursor [M + Na]+ molecular ion from metaldehyde-d16 allowed multi-stage MS/MS/MS experiments to be performed and the result is as shown in insert (v), Fig. 2b, which unambiguously confirms the source of the m/z 71 product ion. Like metaldehyde, the deuterated metaldehyde-d16 species also formed adducts with ammonium ions at m/z 210.
The characterized sodiated [M + Na]+ molecular ions (m/z 199) provided a direct means to detect and quantify metaldehyde in water. This was accomplished by generating a calibration curve obtained using the collisonally activated fragment ion (m/z 71) intensity of the sodiated [M + Na]+ metaldehyde molecular ion at m/z 199 (Supplementary Figure S2, Supplementary Information). The limit of detection (LOD) was determined to be 2.69 ng/mL, which is above the EU regulated LOD value for metaldehyde in water (Table 1). The LOD was determined as the concentration that produces a signal more than three times greater than the standard deviation plus the mean value of the blank (in MS/MS mode). The sensitivity and selectivity of the PS-MS method can be enhanced by exploring chemical reactions that form stable adducts. To this end a more robust ionization mechanism of metaldehyde was developed in the form of reactive paper spray ionization.
Reactive-PS-MS: Characterization and identification of protonated metaldehyde molecular ion species using formic acid
From the results observed in Fig. 2, it can be hypothesized that the sodium [Na]+ and ammonium [NH4]+ ions masked the protonation of metaldehyde. The introduction of reactive reagents in the PS spray solvent can improve the selective detection of metaldehyde in water; when used in combination with tandem MS, this approach can provide the confirmation needed to identify the presence of a particular substance in a complex mixture. This objective was achieved by adding acidified water (0.1% formic acid) to the methanol spray solvent MeOH:(H2O + 0.1% formic acid) (1:1, v/v). The addition of the acidified water greatly suppressed cationization (i.e. [Na]+ and [NH4]+ adduction) and aided protonation. The resultant PS-MS mass spectrum recorded when 5 μg of metaldehyde in 1 μL of deionized water was deposited on the paper substrate using MeOH:(H2O + 0.1% formic acid) (1:1, v/v) as the PS solvent is shown in Fig. 3. An intense, intact protonated molecular ion [M + H]+ of metaldehyde at m/z 177, including a major fragment ion at m/z 149, were observed in the single stage MS analysis (Fig. 3a). This fragment ion (m/z 149) appears to be formed from the elimination of ethylene (CH2 = CH2, MW 28 Da), even prior to collisional activation suggesting a ring opening/rearrangement process in the presence of formic acid.
This observation was further investigated in two experiments: (i) studies of gas-phase fragmentation patterns in tandem MS experiments and (ii) detection of paraldehyde under acidified spray solvent conditions. First, the structure of the protonated metaldehyde and its dissociation behavior were characterized after collisional activation. Insert (i) of Fig. 3a shows the product ion MS/MS mass spectra of the protonated metaldehyde. Unlike the sodiated molecular ion, which fragmented to give sodiated acetaldehyde (Fig. 2), the protonated metaldehyde species dissociates predominantly via the loss of CH2 = CH2 to yield a product ion peak at m/z 149. This fragmentation pathway indicates that the ion at m/z 149, observed in the full MS spectrum, is related to the metaldehyde protonated species and supports our suggestion that the sodiated molecular ions are formed with minimal internal energy deposition. This behavior was also observed using acidified spray solvent for the isotopically labelled metaldehyde-d16, yielding protonated molecular ions with a fragmentation pathway that also suggests a similar ring opening has occurred (see Supplementary Figure S3, Supplementary Information). In addition, CID of m/z 149 directly from the solution was compared with the fragmentation of gas-phase m/z 149 ion formed from MS2 of m/z 177 ion. The two spectra are similar where the mass of the main neutral loss is 28 Da providing an abundant ion peak at m/z 121 (Fig. S4, Supplementary Information). This result suggests that the m/z 149 ion generated in solution is the same in structure as the m/z 149 ion produced in gas-phase under CID. The second experiment to confirm the observed behavior of the protonated metaldehyde involved the use of paraldehyde, a cyclic trimer of acetaldehyde (metaldehyde being the corresponding tetramer). Figure 3b shows the positive ion mode mass spectrum of paraldehyde obtained when 5 μg of the sample was deposited on the paper substrate and sampled by using MeOH:(H2O + 0.1% formic acid) (1:1, v/v) as the spray solvent. A stable protonated molecular ion [M + H]+ of paraldehyde at m/z 133 was observed. The structure of the protonated paraldehyde species was confirmed from CID fragmentation patterns as shown in insert (ii) (Fig. 3b) where the molecular ion yields an intense fragment ion at m/z 89 owing to the neutral loss of acetaldehyde (MW 44 Da). Like metaldehyde, the fragment ion at m/z 89 was observed in the single stage MS experiment. Other signals were also observed such as at m/z 223 and m/z 164 in Fig. 3a,b respectively and their origins are not known.
The fragmentation pathway (m/z 177 → 149) for the protonated ion type was used to quantify metaldehyde in water (see Supplementary Figure S5, Supplementary Information). Using a commercial linear ion trap mass spectrometer, the LOD was determined to be 0.05 ng/mL. This quantitative analysis of metaldehyde in water was achieved from the metaldehyde calibration curve obtained with PS-MS using MeOH:(H2O + 0.1% formic acid) (1:1, v/v) as the PS solvent (Supplementary Figure S5). Following procedures established using LC-MS, deuterated atrazine-d5 (3 ppb, m/z 221 → 179) was chosen as the internal standard17,63. Monitoring the analyte-to-internal standard ratio (A/IS) as a function of analyte concentration yielded good linearity (R2 > 0.99), precision (RSD < 10%) and >fifty-fold decrease in the detection limit for metaldehyde in water compared with normal PS-MS, which utilized sodiated ions in the quantification process (Table 1).
Direct metaldehyde quantitation in environmental water samples
Direct analysis of metaldehyde in a complex, raw, environmental water matrix using PS-MS was investigated without any sample preparation. The two water samples were collected directly from Abberton reservoir (Essex, UK) and Chigwell brook (Essex, UK) without any filtration except for large objects (>3 cm). Each sample had water turbidity levels of ~1.8 and ~0.79 NTU, total organic carbon ~6.5 and ~3.5 mg/L, and, pH ~8.35 and ~8.36, respectively. A volume of ~10 μL from each raw sample was deposited onto the paper substrate and analyzed using a commercial benchtop mass spectrometer in positive ion mode. Figure 4 shows the recorded mass spectrum for analysis of the raw water samples (Chigwell Raw and Abberton Raw supplied by Northumbrian Water Ltd.) using either MeOH or MeOH:(H2O + 0.1% formic acid) (1:1, v/v) as the PS spray solvent. Moderately intense protonated molecular ions of metaldehyde [M + H]+ at m/z 177 were observed in both water samples, and confirmed by MS/MS CID experiments (insert (i) & (ii) in Fig. 4) for the reactive experiment, which utilized an acidic spray solvent. Expectedly the presence of metaldehyde could not be confirmed in the same water samples when analyzed with the ‘normal PS-MS’ using methanol as the spray solvent.
The results obtained for the direct analysis of metaldehyde in water are apparent of the relatively lower sensitivity (higher LOD) of metaldehyde detection using the normal PS-MS method. The detection limit for sodiated metaldehyde is <3 ng/mL but is not suitable for in situ analysis due to the relatively high detection limit and the potential for salt concentration variations, which are likely to be encountered in the environment. Doping a reactive agent into the PS spray solvent enables reactions to occur at the sampling spot concurrently with mass spectra acquisition to aid both sensitivity and selectivity for target molecules present in complex mixtures. As such, experiments of this type (reactive PS-MS) were employed in this study to improve the detection of metaldehyde in water samples by more than an order of magnitude. Although the inclusion of a trace amount of acid is common practice for MS techniques to aid protonation; the addition of the acidified reagent in this case leads to ring opening (Fig. 5), hence the reactive nature of this process. We attribute the high sensitivity of the protonated ion type to the occurrence of only one major fragment ion in CID. The ability to form new ion type(s) from metaldehyde simply by adding reactive reagents (i.e. formic acid) into the PS spray solvent introduces an opportunity to differentiate metaldehyde from other potentially interfering ions having the same nominal mass. This advantage is particularly important for field metaldehyde analysis in which the selectivity of the paper spray method can be increased by studying the fragmentation patterns of sodiated (formed using neutral spray solvent) and protonated (formed using acidified spray solvent) metaldehyde species.
To understand this process (i.e., why [M+H]+ fragments differently than [M+Na]+) it was necessary to investigate the structure/nature of the suspected ring “opening” product formed in the presence of formic acid. As indicated in the results section, the elimination of 28 Da from metaldehyde was assigned to a loss of CH2=CH2 neutral species as illustrated in Fig. 5. This proposal is supported by the failure of acidified metaldehyde to react with hydroxylamine, both in solution and in-situ during reactive paper spray experiments. Product C is presumably formed via an internal proton hopping process and explains why both gas-phase CID and solution-phase rearrangements occur via a common ethylene loss.
PS-MS performed in the tandem mass spectrometry mode can reduce the effect of matrix ion suppression. For quantification purposes, a decision needs to be made as to which ions within the mixture should be subjected to collisional activation. In this respect, performing real time chemical reactions onsite will offer an efficient means to eliminate unrelated matrix ions. The generation of a charged product is expected to improve ionization efficiency of analyte(s) of interest in a complex mixture such as the protonation of metaldehyde in water. The combined reaction/ionization process is tested in this study for the analysis of metaldehyde (Fig. 6). As such both ionization efficiency and molecular selectivity can be improved by the addition of acidified reagents that can yield protonated molecular ions [M + H]+ for targeted analysis and quantification of metaldehyde in water samples (Fig. 3).
The ability to detect and characterize metaldehyde in raw water samples collected from natural water courses has been demonstrated. The concentration of metaldehyde in both water samples was cross-validated and confirmed to be <0.1 μg/L (the EU limit) using LC-MS. The detection limit obtained by PS-MS (Table 1) suggests that it could be suitable for the rapid detection of metaldehyde in raw water, although it registers higher values than those that can be obtained from GC- and LC-MS analytical methods17,18. With the capability of PS-MS to perform in-situ analyses of unmodified samples, the methodology described in this study shows promise for use in routine onsite investigative applications where regular monitoring or rapid screening is required. In this experiment ~10 μL from each sample was deposited on the paper substrate and analyzed using PS-MS. Figure 4 shows the recorded mass spectra for Abberton Raw (a,b) and Chigwell Raw (c,d). Moderately intense protonated molecular ions [M + H]+ of metaldehyde were observed for the reactive experiment and confirmed using MS/MS CID data as shown in Fig. 4, inserts (i) & (ii). The identification of the metaldehyde molecule in both water samples demonstrates the utility of the PS-MS method for direct, rapid screening with little or no sample preparation.
In summary, rapid and direct analysis of metaldehyde has been described using paper spray mass spectrometry. Sodiated [M + Na]+ and protonated [M + H]+ molecular ions produced under two different spray conditions (i.e. acidified MeOH:(H2O + 0.1% formic acid) (1:1, v/v) and normal MeOH PS solvents) were characterized in which [M+Na]+ species were identified to fragment through sequential losses of dimeric and monomeric acetaldehyde neutral species, whereas [M + H]+ dissociates via the elimination of ethylene. Quantitation of metaldehyde was achieved at low concentration (0.05 ng/mL for [M + H]+ and 2.69 ng/mL for [M + Na]+) in water using the reactive PS ionization method with acidified spray solvent. The MS/MS experiment provides a powerful means of qualitative analysis and confirmation of metaldehyde in water. The generation of different ion types in the specified spray conditions can offer an opportunity to readily discriminate (in the field) against other background ions with similar molecular weights since it is unlikely for a particular ion to fragment in a similar fashion as metaldehyde when using sodiated versus protonated parent ions in MS/MS. The demonstrated detection limit shows promise for the direct detection of metaldehyde in water at regulatory levels. Future work will involve further investigation/validation with untreated environmental water samples and pre-determined mock samples of varying water quality to determine potential ion suppression effects64 with a view to onsite in-situ analysis of metaldehyde and related environmental contaminants using a portable mass spectrometer. The objective is to translate the reactive methodology demonstrated with a commercial benchtop system, to a portable MS platform. Recent reports that couple ambient ionization methods, including PS, with portable mass spectrometers are promising65,66,67,68,69. In this respect, portable ion trap technology is often preferred as tandem analysis can be performed in time, without increase in instrument footprint. However with any portable system there is an inevitable trade-off between portability/field ruggedness and performance. For the purpose of onsite testing, in the context of water analysis, the trade-off is not as severe since the mass spectrometer can be confined to a vehicle without stringent restrictions on weight and power. The overarching goal is to achieve timely analysis, allowing near instant decisions to be made. Under current procedures, it can take up to 48 hours from sample collection until a determination is made. It is expected that such a portable setup will provide rapid analysis, being suitable for pre-screening and identifying local sources of pesticide contamination to inform operational decisions. Furthermore, due to the generic nature of MS, this methodology can be extended to other water pollutants that are of concern in the environment. As such, the results are significant beyond the analysis of metaldehyde discussed herein as they represent a means for rapid analysis of other environmental contaminants in water.
When coupled with a miniature mass spectrometer, the directness of the PS-MS experiment itself and the reactive alternative make for a potentially attractive on-site technique for water analysis and environmental monitoring. Other techniques such as the ‘leaf spray’ variant of the paper spray experiment40,41 can benefit by adopting this method for the analysis and determination of metaldehyde and other chemicals of concern on crops such as vegetables that may have been treated with pesticides and/or molluscicide18,37.
Chemicals, reagents and materials
HPLC grade methanol, formic acid and model compounds (i.e., metaldehyde and paraldehyde 99.9% purity) were purchased from Sigma-Aldrich (UK). The deuterium labeled standards, metaldehyde-d16 and atrazine-d5, were purchased from QMX laboratories (Essex, UK) while laboratory grade deionized water was purchased from Reagent Chemicals (Cheshire, UK). The chromatography paper used as the sample substrate was grade I Whatman filter paper (Whatman International Ltd., Maidstone, UK). The water samples (Abberton Raw & Chigwell Raw) were supplied by Northumbrian Water (Durham, UK).
Sample preparation was reduced to the dilution of model compounds to the desired concentrations whilst no sample preparation was performed for the raw water samples (Abberton Raw & Chigwell Raw). Each model compound was diluted in methanol/water (1:1 v/v) to 1000ppm stock solution and serially diluted in deionized water to the desired concentration so that appropriate ion abundances might be recorded; environmental water samples were used as supplied, from Northumbrian Water Ltd. (Durham, UK), without any modification or pre-concentration. From each solution, the 10 sample was deposited onto the filter paper surface, using a pipette and analyzed directly without any sample preparation. In all of the PS-MS experiments performed approximately 10 μL of pure methanol was used as the spray solvent (unless otherwise stated).
All experiments were performed using a linear ion trap (LTQ) mass spectrometer (Thermo Fisher Scientific, San Jose, CA USA), tuned for optimum detection of the precursor ion of interest. The temperature of the MS capillary inlet was typically set at 200 °C. The tube lens voltage was set at 70 V and the capillary voltage maintained at 20 V in positive mode. The filter paper was cut manually into equilateral triangles with ~5 mm sides using scissors. The paper spray substrate was held by a copper clip so that the vertex was ~3 mm away from the inlet of the mass spectrometer. The sample solution was deposited onto the paper substrate followed by the application of an electric potential of 3.5 kV in positive ion mode. The mass spectrometer used is equipped with a differentially pumped atmospheric pressure inlet which acts to draw the PS ion plume into the vacuum system for mass analysis (Fig. 1).
It is important to note that in the paper spray experiments no carrier gas is required, instead a plume of ions is generated by the application of a potential on the paper with the sample and the spray solvent as shown in Fig. 1. Tandem mass spectrometry (MS/MS) was performed on the molecular ions of interest for structural elucidation allowing analyte identification using collision-induced dissociation (CID). An isolation window of 0.1–1.5 Th (mass/charge units) and normalized collision energy of 15–40% (manufacturers unit) were used.
How to cite this article: Maher, S. et al. Direct Analysis and Quantification of Metaldehyde in Water using Reactive Paper Spray Mass Spectrometry. Sci. Rep. 6, 35643; doi: 10.1038/srep35643 (2016).
Caloni, F., Cortinovis, C., Rivolta, M. & Davanzo, F. Suspected poisoning of domestic animals by pesticides. Sci. Total Environ. 539, 331–336 (2016).
Bleakley, C., Ferrie, E., Collum, N. & Burke, L. Self-poisoning with metaldehyde. Emerg. Med. J. 25, 381–382 (2008).
Stuart, M., Lapworth, D., Crane, E. & Hart, A. Review of risk from potential emerging contaminants in UK groundwater. Sci. Total Environ. 416, 1–21 (2012).
Richardson, S. D. & Ternes, T. A. Water analysis: emerging contaminants and current issues. Anal. Chem. 86, 2813–2848 (2014).
Richardson, S. D. & Postigo, C. In Emerging organic contaminants and human health Springer-Verlag: Heidelberg, Berlin, pp 93–1376 (2012).
Hallett, K. C., Atfield, A., Comber, S. & Hutchinson, T. H. Developmental toxicity of metaldehyde in the embryos of Lymnaea stagnalis (Gastropoda: Pulmonata) co-exposed to the synergist piperonyl butoxide. Sci. Total Environ. 543, 37–43 (2016).
Luzardo, O. P. et al. Methodology for the Identification of 117 Pesticides Commonly Involved in the Poisoning of Wildlife Using GC–MS-MS and LC–MS-MS. J. Anal. Toxicol. bku009 (2014).
Ruiz-Suárez, N. et al. Continued implication of the banned pesticides carbofuran and aldicarb in the poisoning of domestic and wild animals of the Canary Islands (Spain). Sci. Total Environ. 505, 1093–1099 (2015).
Reregistration Eligibility Decision for Metaldehyde. U.S. Environmental Protection Agency Web Archive [Online], July 27, 2006. http://archive.epa.gov/pesticides/reregistration/web/pdf/metaldehyde_red.pdf (accessed December 16, 2015).
Jones, A. & Charlton, A. Determination of metaldehyde in suspected cases of animal poisoning using gas chromatography-ion trap mass spectrometry. J. Agric. Food Chem. 47, 4675–4677 (1999).
WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification 2009. World Health Organization. Stuttgart, Germany (2010).
Dolan, T., Howsam, P., Parsons, D. J. & Whelan, M. J. Is the EU drinking water directive standard for pesticides in drinking water consistent with the precautionary principle? Environ. Sci. Technol. 47, 4999–5006 (2013).
Kallis, G. & Butler, D. The EU water framework directive: measures and implications. Water Policy 3, 125–142 (2001).
Kay, P. & Grayson, R. Using water industry data to assess the metaldehyde pollution problem. Water Environ. J. 28, 410–417 (2014).
Zhang, H., Wang, C., Xu, P. & Ma, Y. Analysis of molluscicide metaldehyde in vegetables by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry. Food Addit. Contam. A 28, 1034–1040 (2011).
Maher, S., Jjunju, F. P. & Taylor, S. Colloquium: 100 years of mass spectrometry: Perspectives and future trends. Rev. Mod. Phys. 87, 113 (2015).
The determination of metaldehyde in waters using chromatography with mass spectrometric detection. Environment Agency [Online], 2009. https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/316782/Metaldehyde-226b.pdf (accessed January 10, 2016).
Li, C., Wu, Y.-L., Yang, T. & Zhang, Y. Determination of Metaldehyde in Water by SPE and UPLC–MS–MS. Chromatographia 72, 987–991 (2010).
Cooks, R. G., Ouyang, Z., Takats, Z. & Wiseman, J. M. Ambient mass spectrometry. Science 311, 1566–1570 (2006).
Cheng, S.-C., Jhang, S.-S., Huang, M.-Z. & Shiea, J. Simultaneous Detection of Polar and Nonpolar Compounds by Ambient Mass Spectrometry with a Dual Electrospray and Atmospheric Pressure Chemical Ionization Source. Anal. Chem. 87, 1743–1748 (2015).
Nemes, P. & Vertes, A. Ambient mass spectrometry for in vivo local analysis and in situ molecular tissue imaging. TrAC, Trends Anal. Chem. 34, 22–34 (2012).
Wei, Y. et al. Tissue spray ionization mass spectrometry for rapid recognition of human lung squamous cell carcinoma. Sci. Rep. 5 (2015).
Harris, G. A., Galhena, A. S. & Fernandez, F. M. Ambient sampling/ionization mass spectrometry: applications and current trends. Anal. Chem. 83, 4508–4538 (2011).
Monge, M. E., Harris, G. A., Dwivedi, P. & Fernández, F. M. . Mass spectrometry: recent advances in direct open air surface sampling/ionization. Chem. Rev. 113, 2269–2308 (2013).
Nyadong, L., Green, M. D., De Jesus, V. R., Newton, P. N. & Fernández, F. M. Reactive desorption electrospray ionization linear ion trap mass spectrometry of latest-generation counterfeit antimalarials via noncovalent complex formation. Anal. Chem. 79, 2150–2157 (2007).
Harris, G. A., Nyadong, L. & Fernandez, F. M. Recent developments in ambient ionization techniques for analytical mass spectrometry. Analyst 133, 1297–1301 (2008).
Liu, X.-P. et al. Direct and Convenient Mass Spectrometry Sampling with Ambient Flame Ionization. Sci. Rep. 5 (2015).
Kozlowski, R. L., Mitchell, T. W. & Blanksby, S. J. A rapid ambient ionization-mass spectrometry approach to monitoring the relative abundance of isomeric glycerophospholipids. Sci. Rep. 5 (2015).
Musah, R. A. et al. A high throughput ambient mass spectrometric approach to species identification and classification from chemical fingerprint signatures. Sci. Rep. 5 (2015).
Takats, Z., Wiseman, J. M., Gologan, B. & Cooks, R. G. Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 306, 471–473 (2004).
Chen, H., Wortmann, A., Zhang, W. & Zenobi, R. Rapid In Vivo Fingerprinting of Nonvolatile Compounds in Breath by Extractive Electrospray Ionization Quadrupole Time‐of‐Flight Mass Spectrometry. Angew. Chem. 119, 586–589 (2007).
Zhang, H. et al. Direct characterization of bulk samples by internal extractive electrospray ionization mass spectrometry. Sci. Rep. 3 (2013).
Chen, H., Yang, S., Wortmann, A. & Zenobi, R. Neutral desorption sampling of living objects for rapid analysis by extractive electrospray ionization mass spectrometry. Angew. Chem. 46, 7591–7594 (2007).
Chen, H. et al. Sensitive detection of native proteins using extractive electrospray ionization mass spectrometry. Angew. Chem. 49, 3053–3056 (2010).
Li, M. et al. Facilitated diffusion of acetonitrile revealed by quantitative breath analysis using extractive electrospray ionization mass spectrometry. Sci. Rep. 3 (2013).
Li, X., Hu, B., Ding, J. & Chen, H. Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry. Nat. Protoc. 6, 1010–1025 (2011).
Jjunju, F. P. et al. Analysis of polycyclic aromatic hydrocarbons using desorption atmospheric pressure chemical ionization coupled to a portable mass spectrometer. J. Am. Soc. Mass Spectrom. 26, 271–280 (2015).
Jjunju, F. P. et al. Hand-Held Portable Desorption Atmospheric Pressure Chemical Ionization Ion Source for in Situ Analysis of Nitroaromatic Explosives. Anal. Chem. 87, 10047–10055 (2015).
Yang, S. et al. Detection of melamine in milk products by surface desorption atmospheric pressure chemical ionization mass spectrometry. Anal. Chem. 81, 2426–2436 (2009).
Cody, R. B., Laramée, J. A. & Durst, H. D. Versatile new ion source for the analysis of materials in open air under ambient conditions. Anal. Chem. 77, 2297–2302 (2005).
Nilles, J. M., Connell, T. R. & Durst, H. D. Quantitation of chemical warfare agents using the direct analysis in real time (DART) technique. Anal. Chem. 81, 6744–6749 (2009).
Li, L.-P. et al. Applications of ambient mass spectrometry in high-throughput screening. Analyst 138, 3097–3103 (2013).
Wu, C., Dill, A. L., Eberlin, L. S., Cooks, R. G. & Ifa, D. R. Mass spectrometry imaging under ambient conditions. Mass Spectrom. Rev. 32, 218–243 (2013).
Soparawalla, S., Tadjimukhamedov, F. K., Wiley, J. S., Ouyang, Z. & Cooks, R. G. In situ analysis of agrochemical residues on fruit using ambient ionization on a handheld mass spectrometer. Analyst 136, 4392–4396 (2011).
Wang, H., Liu, J., Cooks, R. G. & Ouyang, Z. Paper Spray for Direct Analysis of Complex Mixtures Using Mass Spectrometry. Angew. Chem. 49, 877–880 (2010).
Manicke, N. E., Abu-Rabie, P., Spooner, N., Ouyang, Z. & Cooks, R. G. Quantitative analysis of therapeutic drugs in dried blood spot samples by paper spray mass spectrometry: an avenue to therapeutic drug monitoring. J. Am. Soc. Mass Spectrom. 22, 1501–1507 (2011).
Espy, R. D., Manicke, N. E., Ouyang, Z. & Cooks, R. G. Rapid analysis of whole blood by paper spray mass spectrometry for point-of-care therapeutic drug monitoring. Analyst 137, 2344–2349 (2012).
Yang, Q. et al. Direct and quantitative analysis of underivatized acylcarnitines in serum and whole blood using paper spray mass spectrometry. Anal. Bioanal. Chem. 404, 1389–1397 (2012).
Wang, H. et al. Direct analysis of biological tissue by paper spray mass spectrometry. Anal. Chem. 83, 1197–1201 (2011).
Zhang, Y., Ju, Y., Huang, C. & Wysocki, V. H. Paper spray ionization of noncovalent protein complexes. Anal. Chem. 86, 1342–1346 (2014).
Taverna, D., Di Donna, L., Mazzotti, F., Policicchio, B. & Sindona, G. High‐throughput determination of Sudan Azo‐dyes within powdered chili pepper by paper spray mass spectrometry. J. Mass Spectrom. 48, 544–547 (2013).
Mazzotti, F. et al. Evaluation of dialdehydic anti-inflammatory active principles in extra-virgin olive oil by reactive paper spray mass spectrometry. Int. J. Mass Spectrom. 352, 87–91 (2013).
Zhang, Z., Cooks, R. G. & Ouyang, Z. Paper spray: a simple and efficient means of analysis of different contaminants in foodstuffs. Analyst 137, 2556–2558 (2012).
Deng, J. & Yang, Y. Chemical fingerprint analysis for quality assessment and control of Bansha herbal tea using paper spray mass spectrometry. Anal. Chim. Acta 785, 82–90 (2013).
Li, A., Wei, P., Hsu, H.-C. & Cooks, R. G. Direct analysis of 4-methylimidazole in foods using paper spray mass spectrometry. Analyst 138, 4624–4630 (2013).
Hamid, A. M. et al. Rapid discrimination of bacteria by paper spray mass spectrometry. Anal. Chem. 86, 7500–7507 (2014).
Reeber, S. L., Gadi, S., Huang, S.-B. & Glish, G. L. Direct analysis of herbicides by paper spray ionization mass spectrometry. Anal. Methods 7, 9808–9816, doi: 10.1039/C5AY02125A (2015).
Shen, L., Zhang, J., Yang, Q., Manicke, N. E. & Ouyang, Z. High throughput paper spray mass spectrometry analysis. Clin. Chim. Acta 420, 28–33 (2013).
Narayanan, R., Sarkar, D., Cooks, R. G. & Pradeep, T. Molecular ionization from carbon nanotube paper. Angew. Chem. 53, 5936–5940 (2014).
Zhang, C. & Manicke, N. E. Development of a paper spray mass spectrometry cartridge with integrated solid phase extraction for bioanalysis. Anal. Chem. 87, 6212–6219 (2015).
Damon, D. E. et al. 2D wax-printed paper substrates with extended solvent supply capabilities allow enhanced ion signal in paper spray ionization. Analyst 141, 3866–3873 (2016).
Espy, R. D., Muliadi, A. R., Ouyang, Z. & Cooks, R. G. Spray mechanism in paper spray ionization. Int. J. Mass Spectrom. 325, 167–171 (2012).
Busquets, R., Kozynchenko, O. P., Whitby, R. L., Tennison, S. R. & Cundy, A. B. Phenolic carbon tailored for the removal of polar organic contaminants from water: A solution to the metaldehyde problem? Water Res. 61, 46–56 (2014).
Vega, C., Spence, C., Zhang, C., Bills, B. J. & Manicke, N. E. Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry. J. Am. Soc. Mass Spectrom 27, 726–734 (2016).
Jjunju, F. P. et al. In situ analysis of corrosion inhibitors using a portable mass spectrometer with paper spray ionization. Analyst 138, 3740–3748 (2013).
Bag, S., Hendricks, P., Reynolds, J. C. & Cooks, R. Biogenic aldehyde determination by reactive paper spray ionization mass spectrometry. Anal. Chim. Acta 860, 37–42 (2015).
Ma, Q. et al. Rapid analysis of synthetic cannabinoids using a miniature mass spectrometer with ambient ionization capability. Talanta 142, 190–196 (2015).
Ma, X. & Ouyang, Z. Ambient ionization and miniature mass spectrometry system for chemical and biological analysis. TrAC Trends Anal. Chem. doi: 10.1016/j.trac.2016.04.009 – in press (2016).
Snyder, D. T., Pulliam, C. J., Ouyang, Z. & Cooks, R. G. Miniature and fieldable mass spectrometers: recent advances. Anal. Chem. 88, 2–29 (2015).
The authors thank Northumbrian Water (Durham, UK) for supplying us with raw water samples used in this study. F. P. M. J. thanks the Department of Electrical Engineering and Electronics, University of Liverpool, UK for a study grant. The NWO (Netherlands) are acknowledged for support of S.U.S (via the TA-COAST program). A. K. B. T. acknowledges funding from The Ohio State University start-up funds.
The authors declare no competing financial interests.
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Maher, S., Jjunju, F., Damon, D. et al. Direct Analysis and Quantification of Metaldehyde in Water using Reactive Paper Spray Mass Spectrometry. Sci Rep 6, 35643 (2016). https://doi.org/10.1038/srep35643
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