Obesity causes changes in microbiota composition and an altered gut microbiota can transfer obesity-associated phenotypes from donors to recipients. Obese Rongchang pigs (RP) exhibited distinct fiber characteristics and lipid metabolic profiles in their muscle compared with lean Yorkshire pigs (YP). However, whether RP have a different gut microbiota than YP and whether there is a relationship between the microbiota and muscle properties are poorly understood. The present study was conducted to test whether the muscle properties can be transferred from pigs to germ-free (GF) mice. High-throughput pyrosequencing confirms the presence of distinct core microbiota between pig breeds, with alterations in taxonomic distribution and modulations in β diversity. RP displayed a significant higher Firmicutes/Bacteroidetes ratio and apparent genera differences compared with YP. Transplanting the porcine microbiota into GF mice replicated the phenotypes of the donors. RP and their GF mouse recipients exhibited a higher body fat mass, a higher slow-contracting fiber proportion, a decreased fiber size and fast IIb fiber percentage and enhanced lipogenesis in the gastrocnemius muscle. Furthermore, the gut microbiota composition of colonized mice shared high similarity with their donor pigs. Taken together, the gut microbiota of obese pigs intrinsically influences skeletal muscle development and the lipid metabolic profiles.
Specialized microbial communities inhabit a majority of the epithelial surfaces of our body, such as the skin, mucosal surfaces and gastrointestinal tract, with by far the greatest number of bacterial cells in the distal gut1. The mammalian distal gut microbiome exceeds the size of the mammalian nuclear genome by two orders of magnitude and has the potential to add a broad range of biological functions that the host could not otherwise perform2. This commensal microbiota plays a major role in maintaining human health, providing nutrients, shaping the immune system and modulating gastrointestinal development; indeed, it is sometimes referred to as our “forgotten organ”3. The gut microbiota is highly variable from individual to individual and in different body sites in a single host. Environmental and stochastic factors strongly affect the composition of the microbiota and accumulating evidences indicate that host genotypes and phenotypes influence and interact with the gut microbiota in various mammals4,5,6. In genetically obese mice and obese patients, there are significant differences in the gut microbiota composition compared with lean controls and these modifications can also be induced by deleting or adding one gene to a model host organism7,8. In studies of monozygotic or dizygotic twin pairs, considerable differences in the gut microbiota composition were found between healthy co-twins and co-twins with obesity or malnourishment9,10. These studies strengthened the notion that a host with different phenotypes or genotypes harbors a distinct gut microbiota. The Rongchang pig (RP) is a typical native pig breed from Southwestern China and is characterized by a high body fat mass, excellent meat quality and high intramuscular fat content in the skeletal muscle11. The Yorkshire pig (YP) is an imported breed and is characterized by a high body lean mass, an inferior meat quality and low lipid storage in the skeletal muscle12. Previous studies also showed that pigs with obese phenotypes have a distinct gut microbiota compared with lean pigs13. Thus, we speculated that there are significant differences in the gut microbiota compositions between YP and RP.
Obesity is a major challenge to health care systems worldwide and is a widely recognized risk factor for various metabolic disorders such as fatty liver and type 2 diabetes. There is substantial evidence suggesting that skeletal muscle properties including fiber characteristics, fiber type distribution and lipid metabolic profile, are closely associated with the presence of obesity14,15. Interestingly, the gut microbiota is reported to be a causal factor of obesity-associated phenotypes, as the metabolic phenotypes can be transferred from donors to recipients through fecal microbiota transplantation9,16,17. Less attention has been paid to the link between the gut microbiota composition and skeletal muscle development and the metabolic profile. Only a limited number of studies suggest that the depletion of gut microbiota leads to increased muscle fatty acid catabolism18. Other accumulating indirect evidence indicates that skeletal muscle development and the metabolic profile are influenced by the ingestion of probiotics/prebiotic19,20. Such findings make it tempting to speculate that a relationship may exist between muscle properties and the gut microbiota. Whether the skeletal muscle properties are transmissible via fecal microbiota transplantation remains unclear. Our previous study demonstrated that RP exhibit a higher slow-contracting fiber percentage and intramuscular fat content compared to an imported pig breed21. Therefore, we hypothesized that the differences in fiber characteristics, fiber type distribution and lipid metabolism in skeletal muscle between pig breeds can be transferred from the pig donors to germ-free (GF) mouse recipients.
The objectives of present study were to investigate differences in the gut microbiota between YP and RP and to determine whether the differences in the skeletal muscle properties are transmissible via fecal microbiota transplantation. Pigs share high similarity with humans in terms of physiology, organ development and disease progression22. Thus, elucidating differences in the gut microbiota between obese pigs and lean pigs and the relationships between the gut microbiota and muscle properties is not only essential for determining the role of the gut microbiota in lipid metabolism and the development of skeletal muscle in pigs but could also reflect the corresponding role of the gut microbiota in humans.
Significant differences in the microbiota between pig breeds and microbiota-associated phenotypes can be transferred from pig donors to GF mouse recipients
A total of 569,248 high quality sequences were obtained from all pig samples (Table S1), with an average of 56,925 sequences per sample and a range of 32,013 to 69,295. These sequences were assigned to 1,796 operational taxonomic units (OTUs), with 809 of those existing in two pig breeds was identified as the core OTUs (Fig. S1A). The core OTUs represented 58.69% of all of the reads. A total of 10,430,445 high-quality sequences were acquired from 12 mouse samples (Table S1). A total of 4,406 OTUs were generated, with 2,114 of those existing in the two groups identified as the core OTUs (Fig. S2A). The core OTUs represented 51.38% of all of the reads. The fecal samples of all of the pigs were dominated by four phyla: Bacteroidetes, Firmicutes, Spirochaetes and Proteobacteria (Fig. 1A). A total of 15 phyla were shared by the two pig breeds (Fig. 1B). Seven phyla (>1% in at least 1 sample) were chosen for the significance analysis and an adjusted p value was adopted. Compared with YP, RP had higher proportions of bacteria in Firmicutes and Spirochaetes and a lower proportion of bacteria in Bacteroidetes (p < 0.05) (Fig. S2). All of the mouse fecal samples were dominated by four phyla: Bacteroidetes, Firmicutes, Proteobacteria and Fusobacteria (Fig. 2A). The results shown in Fig. 2B described the phylotype distribution at the phyla level for the mouse recipients and specific microbiota phyla present in the pig donors were also detected in the mouse recipients. The phyla differences were replicated and a higher proportion of Firmicutes and a lower proportion of Bacteroidetes were observed in RM versus YM (p < 0.05). In addition, YM exhibited a higher proportion of Proteobacteria and a lower proportion of Fusobacteria and Actinobacteria (p < 0.05) (Fig. S4). Only 58.67% of the total sequences derived from pig fecal samples were assigned to 32 known genera. Ten abundant genera (>1%) were detected in the YP samples, while 9 abundant genera were detected in the RP samples. Fig. 1C presents a heatmap showing the abundances of the selected genera (>0.1% in at least 1 sample) across all of the samples, clearly showing that there are apparent differences in the genus distribution between YP and RP fecal microbiota. The proportions of Treponema, YRC22, Oscillospira, Roseburia, Ruminococcus, Paludibacter, Coprococcus and Blautia were higher in RP, whereas the proportions of bacteria in Prevotella, Succinivibrio, Anaerovibrio, Lactobacillus, Bacteroides, Acidaminococcus, Megasphaera and Mitsuokella were higher in YP. Likewise, the bacterial genera distribution differed between Yorkshire pig flora-associated mice (YM) and Rongchang pig flora-associated mice (RM) and several genera differences existing in the pig donors were conserved in the mouse recipients. Roseburia, Ruminococcus and Blautia were more and Prevotella was less represented in RM compared to YM (Fig. 2C). For the α and β diversity analyses, the sequence number for each sample from both pigs and mice was rarefied to 30,000 by randomly subsampling to minimize the variation in sequencing depth among the samples. There were no significant differences in the observed OTUs and the Chao 1 index between the YP and RP samples (p > 0.05) (Fig. S1B,C). As indicated in the pig donors, there were also no significant differences observed for the OTUs and the Chao 1 index between the mouse recipients (p > 0.05) (Fig. S3B,C).
To measure the extent of the similarity between microbiota communities, a principal coordinate analysis (PCoA) based on weighted UniFrac distance metrics was performed. The fecal microbiota from YP and RP divided into two different clusters that separated clearly in the PCoA (Fig. 3). The community structures observed in the YM samples were significantly different from the communities detected in the RM samples (Fig. 3). The PCoA plots showed that YM and RM samples formed two different clusters and that, for each condition, the fecal samples of the mouse recipients formed a cluster that was close to its donor fecal samples. Therefore, overall, the bacterial microbiota showed a marked divergence between YP and RP and the mouse recipients shared high similarity with their pig donors in their gut microbiota.
The two groups of mouse recipients developed different body compositions, fiber characteristics and fiber type distributions
Two groups of GF BALB/C mice were colonized with the fecal suspensions prepared from YP and RP (conventionalization). The two groups of mouse recipients were then named Yorkshire pig flora-associated mice (YM) and Rongchang pig flora-associated mice (RM). Consistent with our previous study21, obese RP had a higher fat mass and a lower lean mass than lean YP (p < 0.05) (Fig. 4A). Consistently, there was a trend toward a higher body fat mass in RM compared to YM (p = 0.0544) (Fig. 4B). Additionally, the fiber diameter and the cross-sectional area (CSA) of the gastrocnemius muscle (GM) trended toward an increase in the lean YP (p = 0.0994) (Fig. 5A). Likewise, there was a trend toward a larger CSA in the GM of YM (p = 0.0832) (Fig. 5B). Owing to the critical role of the fiber type profile in determining the fiber characteristics, the gene expression levels of myosin heavy-chain (MYHC) isoforms in the GM of the pig donors and mouse recipients were measured. As expected, MYH7, which encodes the slow-contracting fiber, was expressed at higher levels in the GM of the RP donors and their GF recipients (p < 0.05). Additionally, the expression of MYH4, which encodes the fast IIb MYHC, was reduced in the GM of RP and RM (p < 0.05) (Fig. 6A,B).
Obese pig-derived microbiota enhances lipogenesis in skeletal muscle
The gut microbiota alters the expression of host genes involved in lipid metabolism23. In the GM, obese RP and their mouse recipients exhibited higher triglyceride (TG) concentrations and lipoprotein lipase (LPL) activity, indicating enhanced lipid deposition (p < 0.05) (Fig. 7A,B). We further analyzed the expression of genes regulating lipid uptake, lipogenesis and lipolysis in the GM. RP exhibited increased expression of acetyl-CoA carboxylase (ACACA) and fatty acid synthase (FASN), but only ACACA abundance was upregulated in RM (p < 0.05). Furthermore, carnitine palmitoyltransferase 1 (CPT1), a transport protein that regulates fatty acid β-oxidation, was reduced in the RP donors and the RM recipients (p < 0.05). Additionally, LPL, which provides fatty acid for tissue utilization and storage, was increased in the RP donors (p < 0.05). There was also a trend towards higher LPL abundance in the RM recipients (p = 0.0864). Moreover, fatty acid translocase/CD36 (FAT/CD36), which imports lipids and lipoproteins, trended toward a higher expression in the RP donors (p = 0.081) and their GF recipients (p = 0.0812). In addition, sterol regulatory element-binding protein-1c (SREBP-1c), a key transcription factor of lipogenesis in skeletal muscle and an activator of ACACA and FASN, was more highly expressed in RP than in YP (p < 0.05). Furthermore, SREBP-1c trended toward increased expression in the RM recipients (p = 0.066). In addition, there were no differences in the expression levels of adipose triglyceride lipase (ATGL) and hormone sensitive lipase (LIPE) between YP and RP or between YM and RM (p > 0.05) (Fig. 8A,B).
The gut microbiota is recognized as a strong determinant of host physiology, especially its critical role in host metabolism24. The causal relationship between the gut microbiota and obesity-associated phenotypes has been extensively studied. Obesity-associated phenotypes are transmissible via fecal microbiota transplantation17,25. There is a wealth of data indicating that the skeletal muscle metabolic profile and the fiber type differed between hosts with metabolic syndrome and healthy individuals26. Some authors suggest that a gut microbiota-muscle axis might exist27. However, there is not enough evidence to indicate a relationship between the gut microbiota and skeletal muscle. Here, we demonstrated that obese RP exhibit distinct and different gut microbial communities compared to lean YP. Furthermore, we showed that the transfer of a porcine microbiota replicates the skeletal muscle properties of the pig donors.
Several factors affect the evolution of the mammalian gut microbiota and host genotypes and phenotypes are considered as main factors contributing to the diversity of the gut microbiota4,28. Consistent with a recent study29, we identified a gut microbiota dominated mainly by Bacteroidetes, Firmicutes, Spirochaetes and Proteobacteria in YP and RP. Studies comparing the gut microbiota between obese and lean animals showed that higher Firmicutes and lower Bacteroidetes levels were associated with obesity13,30,31. We observed similar results in RP and their mouse recipients. In addition, higher levels of Spirochaetes were observed in RP. In the rumen and the termites guts, Spirochaetes is capable of degrading polymers commonly present in plant materials. Certain polymers including xylan, pectin and arabinogalactan were fermentable substrates of Spirochaetes32,33. More recently, Spirochaetes was determined to be positively correlated with the apparent hemicellulose digestibility of pigs34. RP are recognized to be more adaptable to poor dietary conditions than foreign pig breeds35, a fact that may be attributed to the higher Spirochaetes proportion in the gut microbiota. YP and RP are typically lean and obese pigs11,12,36 and RP and their GF mouse recipients exhibited higher body fat masses in the present study. Studies in humans and rodents showed that differences at the genus level play a great role in obesity30,37. Most species of Ruminococcus belong to the Clostridium cluster IV, which has been associated with both obesity and weight loss38,39. Higher levels of Ruminococcus were observed in RP and RM, which is similar to what was observed in obese humans and HF-fed mice40,41. Species from Roseburia and Blautia are major bacteria that produce butyrate and acetic acid, respectively42,43. We found that RP and RM exhibited increased abundance levels of Roseburia and Blautia, which was supported by previous studies showing an elevation in total short chain fatty acid production in obese individual30. Prevotella has been recently reported relative to body weight loss in overweight adolescents38 and was decreased in RP and RM. Previous studies indicated that the all obese gut communities were more similar to each other than to lean gut communities16,31 and we also found that YP and RP fecal microbiota could be divided into two separate clusters based on a beta diversity analysis. The recipient mice shared high similarity in bacterial community structure with their corresponding pig donors, which was consistent with previous studies showing that human and rat microbiota can be transferred to GF mice with striking preservation of structure and diversity37,44. Overall, these data indicated that the gut composition differs between obese RP and lean YP and the microbiota-related phenotypes of pig donors were preserved and transferred to their GF mouse recipients.
In humans, skeletal muscle represents approximately 40% of the body weight and constitutes the largest organ in our body45. During the past decade, it has been increasingly identified that skeletal muscle development and metabolism are closely associated with obesity46. There are differences in the fiber type proportions between lean and obese animals21,47. In the present study, we found that RP and RM exhibited higher amounts of type I fibers and lower amounts of type IIb fibers, consistent with our previous study21. Recent studies demonstrated that muscle hypertrophy, owing to increased type IIb fibers, plays an important role in combating diet-induced obesity and metabolic dysfunction48,49. In agreement, muscle fiber diameter and CSA were lower in RP and a lower CSA was detected in RM. Obesity leads not only to an increased lipid deposition in adipose tissues but also to the infiltration of fat in other tissues, such as the liver and skeletal muscle50. We also found that obese pig donors and their mouse recipients had higher TG content in the GM. Previous studies indicated that lipid storage in non-adipose tissue attributed to an increased uptake of free fatty acid together with a reduced fatty acid catabolism in those tissues51. In this regard, RP and RM exhibited elevated LPL and FAT/CD36 mRNA levels in the GM, indicative of increased fatty acid uptake into skeletal muscle52. Higher ACACA and SREBP-1c and lower CPT-1 mRNA abundance levels were observed in the GM of obese pig donors and RM, indicating that the obese state enhances lipogenesis and inhibits fatty acid catabolism in skeletal muscle51. Collectively, these results indicate that obese state-induced alterations to the gut microbiota affect muscle properties including fiber characteristics, fiber type distribution and lipid metabolism. While previous studies have shown that fecal microbiota transplantations into GF mouse recipients can replicate several aspects of the obesity-associated phenotypes16,17, this is the first demonstration that skeletal muscle fiber proportions and lipid metabolic profiles can be transferred from pig donors to mouse recipients. Our study provides new evidences supporting the existence of a gut microbiota-muscle axis27.
In conclusion, this study demonstrates the large distinction in the gut microbiota composition between obese and lean pigs and reveals the contribution of the gut microbiota to the regulation of fiber characteristics, fiber type distribution and lipid metabolism in skeletal muscle. Taken together, these data demonstrate that obese individuals may harbor a specific gut microbiome that enhances ectopic fat deposition in skeletal muscle and inhibits muscle growth. These findings provide new approaches to intervene host metabolism and animal phenotypes.
All of the experimental procedures and animal care were performed in accordance with the Guide for the Care and Use of Laboratory Animals prepared by the Institutional Animal Care and Use Committee of Sichuan Agricultural University and all of the animal protocols were approved by the Animal Care and Use Committee of Sichuan Agricultural University under permit number DKY-B20131704.
Pigs: Rongchang pigs (RP; n = 5) and Yorkshire pigs (YP; n = 5) were provided by a reservation farm. YP and RP were housed separately in two environmentally controlled room that allowed ad libitum access to water and diet. Starting at 12 weeks of age, the pigs were fed a regular diet for 8 weeks, until euthanasia. The diet (Table S2) was formulated to meet or exceed current NRC (2012) recommendations for all nutrients for 25- to 50-kg pigs. There was no significant difference in the feed intake between pig breeds (data not shown).
Mice: A total of 20 1-day-old germ-free (GF) BALB/C mice were provided by the Department of Laboratory Animal Science of the Third Military Medical University and were used as recipients for the fecal microbiota transplantation. The GF mice were housed in sterile plastic film isolators and were given ad libitum access to sterilized water during the whole course of the experiment. One-day-old mice were breast fed by the GF foster mice until weaning (weaned at 3 weeks of age) and were then fed ad libitum with a sterilized chow diet for 2 weeks post-weaning.
Fecal microbiota transplantation
According to the criteria for donor identification and screening described by Hamilton et al.53, the pigs used in the current study consumed a regular diet without antibiotics and probiotics for 8 weeks prior to feces collection. Spontaneously excreted feces were collected from all of the pigs during the final week prior to the slaughter. To acquire representative fecal material for each breed, parts of the fecal samples derived from pigs within the same breed were mixed and were then used as the fecal inoculum. The remaining feces derived from the pigs were stored at −80 °C until DNA extraction. The stool suspension was prepared as we previously described54. Newborn GF mice (n = 10 for each pig breed) were colonized with 0.05 ml of the porcine fecal suspension using a nasogastric tube and 2-ml aliquot suspensions were spread on the fur of each foster mouse. Two treatments were produced: Rongchang porcine flora-associated mice (RM) and Yorkshire porcine flora-associated mice (YM).
Sample collection from the donors and recipients
Spontaneously excreted fecal samples were collected from 12 male mice (6 mice per treatment) in the last two days prior to euthanasia and were immediately stored at −80 °C until DNA extraction. All of the pigs were euthanized at 20 weeks of age as we previously described55 and all of the mice were euthanized at 5 weeks of age. The GM from the pigs (n = 5 per breed) and mice (n = 6 per group) was collected at euthanasia for further histological and molecular analyses. The remaining intact mice (n = 4 per group) were only used for body composition analyses.
DNA extraction and microbiota analysis
Total DNA was isolated and purified using the QIAamp DNA stool Mini Kit (Qiagen, GmbH Hilden, Germany) modified to contain a bead-beating step. The concentration and purity of the extracted genomic DNA were measured using a NanoDrop ND-1000 spectrophotometer (NanoDrop, Germany). The integrity of the extracted genomic DNA was determined by electrophoresis on a 1% (w/v) agarose gel.
Sequencing and bioinformatics analysis were performed by BGI (Shenzhen, China). Prior to high-throughput sequencing, a DNA library was prepared as previously described56. Briefly, the DNA extracted from the fecal samples was used as a template to amplify the hypervariable regions V3 and V4 of 16S ribosome RNA gene. The primers contained base pair sequence complementary for the V3 and V4 regions and illumine adaptors and molecular barcodes as previously described57. The resulting amplicons were gel purified, quantified, pooled and sequenced using the 250-bp paired-end reads strategy on the Illumina HiSeq 2000 platform.
The resulting sequences were clustered into OTUs using USEARCH drive5 at 97% sequence similarity. The chimeric OTUs were removed using UCHIME v4.2. Representative sequences for each OTU were picked and aligned using QIIME 1.8. The Ribosomal Database Project classifier v2.2 was used to assign a taxonomic rank to each sequence in the representative set. The relative abundance of each OTU was examined at different taxonomic levels. To minimize biases caused by sequencing depth, the number of reads per sample (all pig and mouse samples) was randomly subsampled to 30,000. The alpha and beta diversity calculations and the taxonomic community assessments were performed using QIIME 1.8 scripts.
Carcass compositions of the pigs and body compositions of the mice
To determine the carcass compositions of the pigs, the right side of each carcass was dissected into 4 parts as follows: lean; fat; bone and skin. The lean and fat percentages of the carcass were calculated based on the formula (NY/T 825–2004, Ministry of Agriculture of the People’s Republic of China, 2004) as follows:
Lean percentage (%) = lean weight (kg)/[lean weight (kg) + fat weight (kg) + bone weight (kg) + skin weight (kg)] × 100%.
Fat percentage (%) = fat weight (kg)/[lean weight (kg) + fat weight (kg) + bone weight (kg) + skin weight (kg)] × 100%.
Four mice in each group were chosen for analyzing body compositions. Intact mice were chopped and dried by lyophilization. The dried material was ground to determine the chemical composition of the whole body. Total nitrogen was analyzed using the Kjeldahl method58. The fat content of each intact mouse was measured by extraction with petrol ether overnight using a Soxhlet apparatus.
The GMs, removed from pigs (n = 5) and mice (n = 6), were fixed in a 10% formalin solution, dehydrated and embedded in paraffin wax. Muscle sections were cut to a thickness of 10 μm and were stained with hematoxylin and eosin to observe the morphology of the muscle tissues. Five sections of each sample were photographed using a digital microscope camera (JVC, Yokohama, Japan) linked with a light microscope (Olympus, Tokyo, Japan) at 40X magnification. Image-processing software (Image-Pro Plus 4.5, Silver Spring, MD, USA) was used to score all of the parameters in the sections. A total of 500 fibers from five random fields were measured to calculate the mean values of the fiber diameter and the cross-sectional area. As an indicator of fiber density, the number of fibers was also expressed per mm2 in the GMs. All of the observations were determined by a single experimenter who was blinded to the pig breed and the source of the gut microbiota.
Muscle metabolites concentrations and enzyme activity
The glycogen content of the muscle was measured according to the method previously described59. Briefly, powdered muscle (50 mg) was hydrolyzed in 150 μl of 1 mol/L KOH by heating at 95 °C for 20 min. The samples were incubated at 37 °C for 2 h, centrifuged at 4 °C/16000 × g for 10 min and neutralized with NaOH. The resulting free glycosyl units were determined using D-glucose as a standard and the results are expressed as mg/g muscle tissue. The LPL activity and TG content in the skeletal muscle were measured using a Triglyceride Assay Kit and a Lipoprotein Lipase Assay Kit provided by the Nanjing Jiancheng Bioengineering Institute, China. Approximately 100 mg of frozen muscle was minced, weighed and thoroughly homogenized with 1 ml of ice-cold PBS using a mechanical tissue disrupter. After centrifugation (2500 × g at 4 °C), the supernatants were decanted and saved for assaying LPL activity and the TG concentration was measured in triplicate at the appropriate dilutions. The LPL activity is expressed as U/mg protein of muscle tissue and the TG content is expressed as mmol/g protein of muscle tissue. The total protein content of the supernatant was measured with the Coomassie Brilliant Blue Protein Assay Kit (Nanjing Jiancheng Institute of Bioengineering, China).
Total RNA from the GM was extracted with TRIzol reagent (TaKaRa, Dalian, China) according to manufacturer’s instructions. cDNA was synthesized via reverse transcription, which was performed with 2 μg of total RNA using a PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China). Primers used for the target genes (MYH7, MYH2, MYH4, MYH1, ACACA, FASN, CPT1, LPL, FAT/CD36 ATGL, LIPE and SREBP-1c) were designed using Primer3Plus (Applied Biosystems) based on the certain exon-exon boundaries of published gene sequences of pigs or mice. Quantitative real-time PCR was performed on an ABI Prism 7000 detection system in a two-step protocol with SYBR Green (Applied Biosystems, Foster City, CA, USA). Each 10 μl volume reaction contained 1 μl of cDNA, 5 μl of SYBR Premix Ex Taq TM (2×), 0.2 μl of ROX reference dye (50×), 0.4 μl of each forward and reverse primer and 3 μl of PCR-grade water. The thermal cycling program included a 1-min pre-incubation at 95 °C, followed by 40 cycles of denaturation at 95 °C for 5 s, a 60 °C annealing step for 25 s and an extension at 72 °C for 15 s. The expression of the 18S RNA gene for each species was used as an internal control. All of the experimental sample analyses were run in triplicate. The gene expression data were calculated using the 2−ΔΔCt method and are expressed as the ratio of the expression of targeted genes to the 18S RNA housekeeping gene60. The relative expression of targeted genes in porcine or murine muscle were normalized to the YP or YM group, respectively.
For all of the parameters, the data were tested for significance with the two-sample t-test method using statistic software SAS 9.1 statistic software (SAS Institute Inc., NC, USA) and are expressed as the mean ± SE. To correct for multiple comparisons in the statistical testing, a Bonferroni correction was used to adjust all of the p values. The differences were considered significant when the p values < 0.05. The beta diversity and PCoA plots were produced using weighted UniFrac metrics. The plots were visualized using R software (Package ape). For the heatmap representations, log10-transformation was applied on the genus relative abundance data matrix, which allowed for the visualization of similarities or differences between the samples that affect members of the community that may make up less than 1% of the relative abundance in a sample.
How to cite this article: Yan, H. et al. Gut microbiota can transfer fiber characteristics and lipid metabolic profiles of skeletal muscle from pigs to germ-free mice. Sci. Rep. 6, 31786; doi: 10.1038/srep31786 (2016).
Dethlefsen, L., McFall-Ngai, M. & Relman, D. A. An ecological and evolutionary perspective on human–microbe mutualism and disease. Nature 449, 811–818 (2007).
Ley, R. E., Lozupone, C. A., Hamady, M., Knight, R. & Gordon, J. I. Worlds within worlds: evolution of the vertebrate gut microbiota. Nat Rev Microbiol 6, 776–788 (2008).
O’Hara, A. M. & Shanahan, F. The gut flora as a forgotten organ. EMBO Rep 7, 688–693 (2006).
Spor, A., Koren, O. & Ley, R. Unravelling the effects of the environment and host genotype on the gut microbiome. Nat Rev Microbiol 9, 279–290 (2011).
Pajarillo, E. A. B. et al. Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines. J Microbio 52, 646–651 (2014).
Monira, S. et al. Gut microbiota of healthy and malnourished children in Bangladesh. Front Microbiol 2, 10.3389 (2011).
Ley, R. E., Turnbaugh, P. J., Klein, S. & Gordon, J. I. Microbial ecology: human gut microbes associated with obesity. Nature 444, 1022–1023 (2006).
Vijay-Kumar, M. et al. Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5. Science 328, 228–231 (2010).
Turnbaugh, P. J. et al. A core gut microbiome in obese and lean twins. Nature 457, 480–484 (2009).
Smith, M. I. et al. Gut microbiomes of Malawian twin pairs discordant for kwashiorkor. Science 339, 548–554 (2013).
Zeng, Z., Yu, B., Mao, X. & Chen, D. Effects of dietary digestible energy concentration on growth, meat quality and PPARγ gene expression in muscle and adipose tissues of Rongchang piglets. Meat Sci 90, 66–70 (2012).
Renaudeau, D. & Mourot, J. A comparison of carcass and meat quality characteristics of Creole and Large White pigs slaughtered at 90 kg BW. Meat Sci 76, 165–171 (2007).
Guo, X. et al. Development of a real‐time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Lett Appl Microbiol 47, 367–373 (2008).
Houmard, J. A., Pories, W. J. & Dohm, G. L. Severe obesity: evidence for a deranged metabolic program in skeletal muscle? Exerc Sport Sci Rev 40, 204 (2012).
Eckardt, K., Taube, A. & Eckel, J. Obesity-associated insulin resistance in skeletal muscle: role of lipid accumulation and physical inactivity. Rev Endocr Metab Dis 12, 163–172 (2011).
Turnbaugh, P. J., Bäckhed, F., Fulton, L. & Gordon, J. I. Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe 3, 213–223 (2008).
Turnbaugh, P. J. et al. An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444, 1027–1131 (2006).
Bäckhed, F., Manchester, J. K., Semenkovich, C. F. & Gordon, J. I. Mechanisms underlying the resistance to diet-induced obesity in germ-free mice. P Natl Acad Sci 104, 979–984 (2007).
Murakami, Y. et al. Supplemental epilactose prevents metabolic disorders through uncoupling protein-1 induction in the skeletal muscle of mice fed high-fat diets. Brit J Nutr, 114, 1774–1783 (2015).
Georges, J. et al. The effects of probiotic supplementation on lean body mass, strength and power and health indicators in resistance trained males: a pilot study. J Int Soc Sport Nutr 11, P38 (2014).
Zhang, C. et al. Differential expression of lipid metabolism-related genes and myosin heavy chain isoform genes in pig muscle tissue leading to different meat quality. Animal 9, 1073–1080 (2015).
Swindle, M., Makin, A., Herron, A., Clubb, F. & Frazier, K. Swine as models in biomedical research and toxicology testing. Vet Pathol 49, 344–356 (2012).
Bäckhed, F. et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci 101, 15718–15723 (2004).
Sommer, F. & Bäckhed, F. The gut microbiota—masters of host development and physiology. Nat Rev Microbiol 11, 227–238 (2013).
Tremaroli, V. & Bäckhed, F. Functional interactions between the gut microbiota and host metabolism. Nature 489, 242–249 (2012).
He, J., Watkins, S. & Kelley, D. E. Skeletal muscle lipid content and oxidative enzyme activity in relation to muscle fiber type in type 2 diabetes and obesity. Diabetes 50, 817–823 (2001).
Bindels, L. B. & Delzenne, N. M. Muscle wasting: the gut microbiota as a new therapeutic target? Int J Biochem Cell B 45, 2186–2190 (2013).
Benson, A. K. et al. Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. P Natl Acad Sci 107, 18933–18938 (2010).
Allen, H. K. et al. Antibiotics in feed induce prophages in swine fecal microbiomes. Mbio 2, e00260–00211 (2011).
Schwiertz, A. et al. Microbiota and SCFA in lean and overweight healthy subjects. Obesity 18, 190–195 (2010).
Ley, R. E. et al. Obesity alters gut microbial ecology. Proc Natl Acad Sci 102, 11070–11075 (2005).
Lilburn, T., Schmidt, T. & Breznak, J. Phylogenetic diversity of termite gut spirochaetes. Environ Microbiol 1, 331–345 (1999).
Paster, B. & Canale-Parola, E. Physiological diversity of rumen spirochetes. Appl Environ Microb 43, 686–693 (1982).
Niu, Q. et al. Dynamic Distribution of the Gut Microbiota and the Relationship with Apparent Crude Fiber Digestibility and Growth Stages in Pigs. Sci Rep 5, 9938 (2015).
YANG, L. et al. Intestinal Microbial Community and Its Fingerprint Comparative Analysis between Rongchang, Landrace and Duroc using ERIC-PCR-DGGE. J Domest Anim Ecol 5, 005 (2011).
Knapp, P., Willam, A. & Sölkner, J. Genetic parameters for lean meat content and meat quality traits in different pig breeds. Livest Prod Sci 52, 69–73 (1997).
Duca, F. A. et al. Replication of obesity and associated signaling pathways through transfer of microbiota from obese prone rat. Diabetes 63, 1624–1636 (2014).
Santacruz, A. et al. Interplay between weight loss and gut microbiota composition in overweight adolescents. Obesity 17, 1906–1915 (2009).
Geurts, L. et al. Altered gut microbiota and endocannabinoid system tone in obese and diabetic leptin-resistant mice: impact on apelin regulation in adipose tissue. Front Microbiol 2, 149 (2011).
Ferrer, M. et al. Microbiota from the distal guts of lean and obese adolescents exhibit partial functional redundancy besides clear differences in community structure. Environ Microbiol 15, 211–226 (2013).
Kim, K.-A., Gu, W., Lee, I.-A., Joh, E.-H. & Kim, D.-H. High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway. Plos One 7, e47713 (2012).
Duncan, S. H. et al. Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces. Int J Syst Evol Micr 56, 2437–2441 (2006).
Liu, C., Finegold, S. M., Song, Y. & Lawson, P. A. Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. Int J Syst Evol Micr 58, 1896–1902 (2008).
Turnbaugh, P. J. et al. The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice. Sci Transl Med 1, 6ra14–16ra14 (2009).
Pedersen, B. K. & Febbraio, M. A. Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nat Rev Endocrinol 8, 457–465 (2012).
Maltin, C. A. Muscle development and obesity: Is there a relationship? Organogenesis 4, 158–169 (2008).
Tanner, C. J. et al. Muscle fiber type is associated with obesity and weight loss. Am J Physiol-Endoc M 282, E1191–E1196 (2002).
Harrison, B. C. & Leinwand, L. A. Fighting fat with muscle: bulking up to slim down. Cell Metab 7, 97–98 (2008).
Izumiya, Y. et al. Fast/Glycolytic muscle fiber growth reduces fat mass and improves metabolic parameters in obese mice. Cell Metab 7, 159–172 (2008).
Fraulob, J. C., Ogg-Diamantino, R., Fernandes-Santos, C., Aguila, M. B. & Mandarim-de-Lacerda, C. A. A mouse model of metabolic syndrome: insulin resistance, fatty liver and non-alcoholic fatty pancreas disease (NAFPD) in C57BL/6 mice fed a high fat diet. J Clin Biochem Nutr 46, 212 (2010).
Shulman, G. I. Cellular mechanisms of insulin resistance. J Clin Invest 106, 171 (2000).
Goldberg, I. J., Eckel, R. H. & Abumrad, N. A. Regulation of fatty acid uptake into tissues: lipoprotein lipase-and CD36-mediated pathways. J Lipid Res 50, S86–S90 (2009).
Hamilton, M. J., Weingarden, A. R., Sadowsky, M. J. & Khoruts, A. Standardized frozen preparation for transplantation of fecal microbiota for recurrent Clostridium difficile infection. Am J Gastroenterol 107, 761–767 (2012).
Zeng, B. et al. Effects of Age and Strain on the Microbiota Colonization in an Infant Human Flora-Associated Mouse Model. Curr Microbiol 67, 313–321 (2013).
Jun, H., Daiwen, C. & Bing, Y. Metabolic and Transcriptomic Responses of Weaned Pigs Induced by Different Dietary Amylose and Amylopectin Ratio. Plos One 5, e15110 (2010).
Bruce-Keller, A. J. et al. Obese-type gut microbiota induce neurobehavioral changes in the absence of obesity. Biol Psychiat 77, 607–615 (2015).
Kozich, J. J., Westcott, S. L., Baxter, N. T., Highlander, S. K. & Schloss, P. D. Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Appl Environ Microb 79, 5112–5120 (2013).
Herrich, K. 935–937 (The Association of Official Analytical Chemists Inc, Arlington, 1990).
Hunter, R. W., Treebak, J. T., Wojtaszewski, J. F. & Sakamoto, K. Molecular mechanism by which AMP-activated protein kinase activation promotes glycogen accumulation in muscle. Diabetes 60, 766–774 (2011).
Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3, 1101–1108 (2008).
This study was financially supported by the earmarked fund for the China Agriculture Research System (CARS-36), the Sichuan Province Science and Technology Support Project (2012NZ0001), the National Natural Science Foundation of China (titled The Relationship and Crosstalk Mechanism between Gut Microbiota and Metabolic Characteristics in Pigs), the National Basic Research Program of China (2013CB531406) and the National High Technology Research and Development Program of China (2014AA022209).
The authors declare no competing financial interests.
Electronic supplementary material
About this article
Cite this article
Yan, H., Diao, H., Xiao, Y. et al. Gut microbiota can transfer fiber characteristics and lipid metabolic profiles of skeletal muscle from pigs to germ-free mice. Sci Rep 6, 31786 (2016). https://doi.org/10.1038/srep31786
BMC Veterinary Research (2021)
Microbial Biotechnology (2021)
Introduction of Colonic and Fecal Microbiota From an Adult Pig Differently Affects the Growth, Gut Health, Intestinal Microbiota and Blood Metabolome of Newborn Piglets
Frontiers in Microbiology (2021)
Best Practice & Research Clinical Endocrinology & Metabolism (2021)
Frontiers in Nutrition (2021)