Semi-dwarfing genes have contributed to enhanced lodging resistance, resulting in increased crop productivity. In the history of grain sorghum breeding, the spontaneous mutation, dw1 found in Memphis in 1905, was the first widely used semi-dwarfing gene. Here, we report the identification and characterization of Dw1. We performed quantitative trait locus (QTL) analysis and cloning, and revealed that Dw1 encodes a novel uncharacterized protein. Knockdown or T-DNA insertion lines of orthologous genes in rice and Arabidopsis also showed semi-dwarfism similar to that of a nearly isogenic line (NIL) carrying dw1 (NIL-dw1) of sorghum. A histological analysis of the NIL-dw1 revealed that the longitudinal parenchymal cell lengths of the internode were almost the same between NIL-dw1 and wildtype, while the number of cells per internode was significantly reduced in NIL-dw1. NIL-dw1dw3, carrying both dw1 and dw3 (involved in auxin transport), showed a synergistic phenotype. These observations demonstrate that the dw1 reduced the cell proliferation activity in the internodes, and the synergistic effect of dw1 and dw3 contributes to improved lodging resistance and mechanical harvesting.
A C4 crop, sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop and is the dietary staple of more than 500 million people in 98 countries1,2. This crop is drought tolerant and can be grown in semi-arid conditions, where maize, wheat, and rice cannot be grown because of water scarcity3.
Recently, some agronomically important genes of sorghum have been isolated using comparative genome studies between the sorghum and other crops. In the case of flowering time, PSEUDORESPONSE REGULATOR PROTEIN (SbPRR37)/Ma14, PHYTOCHROME B (SbPHYB)/Ma35, PHYTOCHROME C (SbPHYC)/Ma5, EARLY HEADING DATE 1 (SbEHD1), FLOWERING LOCUS T (sbFT)/SbCN15, SbCN8, SbCN126, CONSTANS (SbCO)7, and GRAIN NUMBER, PLANT HEIGHT AND HEADING DATE 7 (SbGHD7)/Ma68, have been identified based on their synteny or homology with the genes of other plant species. In contrast, however, the cloning and analysis of dwarfing genes has not advanced. It may be that their causal genes, except Dw3, do not correlate to previously identified genes in other plants (see below). In sorghum breeding, the four major unlinked dwarfing genes, Dw1-Dw4, have been combined to reduce plant height to increase lodging resistance and improve mechanized harvesting9. Among these dwarfing genes, Dw3 has been cloned. It is syntenic to maize Brachytic2, which is involved in auxin transport10. The remaining dwarfing genes have not been cloned.
The origin of the dw1 mutation is described in detail11. In brief, Dwarf Yellow Milo, a spontaneous mutant derived from Standard Milo, was found in a field at Memphis in 1905, and then the seeds were distributed to famers11. The mutant and its derivatives were widely accepted from 1906 until the 1920s in the United States11,12. Now, many cultivars of grain sorghums carry the dw1 mutation, indicating its importance for lodging resistance and improved mechanized harvesting. Genetic analyses of dw1 have been performed by several groups13,14,15,16,17, and it was hypothesized that a gibberellin (GA) catabolizing gene, GA 2-oxidase (Sobic.009G230800) might correspond to dw1 because this gene locates to the dw1 locus15,16,17. More recently, however, we revealed that there were no differences in the sequences or expression levels of this GA2ox gene between the two cultivars ‘Tall White Sooner Milo’, carrying Dw1, and ‘Dwarf White Milo’, carrying dw118. We also revealed that a deficiency in GA induces not only dwarfism of culm, but also culm bending, inevitably leading to abnormal plant architecture, which is unsuitable for breeding18. These results indicated that the sorghum dwarfing genes used for its breeding might have no relation to GA deficiency or insensitivity, unlike the rice and wheat dwarfing genes that contributed to the ‘green revolution’, which encode GA synthesis enzymes and signaling proteins19,20. Thus, the cloning of Dw1 would aid in the understanding of a novel molecular mechanism for dwarfism that is useful in breeding. Here, we isolated the Dw1 gene using a QTL analysis and positional cloning, and revealed that Dw1 encodes a novel protein. The loss-of-function in rice and Arabidopsis homologs also induces semi-dwarfism in these plants. A histological analysis of a nearly isogenic line carrying dw1 (NIL-dw1) revealed that the longitudinal parenchymal cell lengths of the internode were almost the same between NIL-dw1 and wildtype, while the number of cells per internode was significantly reduced in NIL-dw1. Furthermore, NIL-dw1dw3, carrying both dw1 and dw3 (involved in auxin transport), showed a synergistic phenotype. Based on these observations, we discuss the interaction between dw1 and dw3 from a view point of crop breeding and cell proliferation.
For the QTL analysis, we used two sorghum cultivars, bmr-6 and SIL-05, showing different culm lengths (CLs). bmr-6, which carries three dwarfing genes, including dw1, has a ~80 cm CL at the heading stage, whereas SIL-05, which probably carries no dwarfing gene, has a CL of ~365 cm (Fig. 1a,d). When we compared the elongation pattern of internodes, the elongation defect in bmr-6 occurred at all internodes in comparison with the SIL-05 internodes (Fig. 1b,c; Supplementary Fig. S1). We produced F2 plants by crossing bmr-6 and SIL-05. The CL values of 185 F2 plants were broadly distributed from 37 to 421 cm (Fig. 1d). We performed a QTL analysis on 96 F2 plants using 162 molecular markers and detected three major QTLs, which were located on chromosomes 6 (qCL-6), 7 (qCL-7), and 9 (qCL-9) (Fig. 1e and Table 1). The bmr-6 allele of all three QTLs shortened the CL (Table 1).
The locations of these three qCLs correspond to those already reported for dw1-dw3; that is, qCL-6 to dw2, qCL-7 to dw3, and qCL-9 to dw1. We sequenced the Dw3 gene and confirmed that the bmr-6 allele has the same mutation previously reported, indicating that qCL-7 depends on the dw3 mutation (Supplementary Fig. S2a). The situation for qCL-6 is more complex because the dw2 mutation is tightly linked to a maturation gene, ma117,21,22, which affects the flowering time and consequently the CL. In fact, a strong QTL of flowering time overlaps this region in the same F2 plants (Supplementary Fig. S3), and bmr-6 carries the mutation allele of ma1, as previously reported4 (Supplementary Fig. S2b), indicating that qCL-6 might be affected by Ma1. Therefore, we focused on the isolation of Dw1 in this study.
For the positional cloning of Dw1, we generated heterozygous inbred families (HIFs), which are kinds of recombinant inbred lines (F5 and F6), carrying a segregating locus of Dw1. In this experiment, we used two HIFs showing clear differences in CL, dependent on Dw1 segregation, for further mapping. The genetic analysis, which used ~10,000 plants in two years, narrowed down a candidate region to 18 kb between markers located at 57.209 Mb and 57.227 Mb (Fig. 2a). To compare the genomic sequences of bmr-6 and SIL-05, we constructed bacterial artificial chromosome (BAC) libraries derived from the two cultivars and screened the clones containing the candidate region. Both sequences predicted five genes in the region (Fig. 2a). In Sobic.009g229800, there was one single nucleotide polymorphism (SNP) inducing a premature stop codon in bmr-6 (Fig. 2a). In Sobic.009g230200, there were four amino acid differences, while the predicted amino acid sequences of the three other genes, Sobic.009g229900, Sobic.009g230000, and Sobic.009g230100, were identical.
We compared the expression levels of these genes using semi-quantitative RT-PCR and RNA-seq analyses on RNAs extracted from elongating internodes (Fig. 2b). The RT-PCR analyses revealed that the expression of Sobic.009g229800 in bmr-6 was slightly lower than that in SIL-05, while the expression of the other genes was almost same in these cultivars, with no detection of Sobic.009g229900 or Sobic.009g230200. The results of RNA-seq supported the above RT-PCR observations. There were similar expression levels of Sobic.009g230100, no, or very low levels, of Sobic.009g229900, Sobic.009g230000, and Sobic.009g230200, and ~50% expression of Sobic.009g229800 in bmr-6 in comparison with SIL-05.
To confirm that Sobic.009g229800 corresponds to qCL-9, we carried out a complementation experiment using cv. Tx430, which carries the allele of bmr-6 at qCL-9. A 4.5 kb DNA fragment containing Sobic.009g229800 of SIL-05 was introduced into Tx430 by Agrobacterium-mediated transformation. The transformants showed the rescued phenotype, whereas the plants transformed with the empty vector showed dwarfism (Fig. 2c–e). These results strongly suggest that Sobic.009g229800 is the causal gene for qCL-9.
Next, we examined sequences of the candidate 18 kb region in ‘Tall White Sooner Milo’, carrying Dw1, and ‘Dwarf White Milo’, carrying dw1, because the genotypes of Dw1 in both cultivars were described in detail previously12,21. Surprisingly, there were only two SNPs in the region between the two cultivars (Supplementary Fig. S4). One was the causal SNP (the loss-of-function allele) of qCL-9 in Sobic.009g229800, which also existed in ‘Dwarf White Milo’, however, ‘Tall White Sooner Milo’ and ‘Standard Milo’ (mother cultivar of Dwarf Milo) contained the SIL-05 allele (the WT allele). Another SNP was found in an intergenic region. There was no SNP in Sobic.009g230200. This result strongly suggests that Sobic.009g229800 is the causal gene for not only qCL-9 but also Dw1.
Molecular analysis and biological activity of DW1
Next, we analyzed the characteristics of Sobic.009g229800/Dw1. Although its predicted amino acid sequence does not contain any significant characterized domains or sequences, a phylogenetic analysis revealed that there are homologs among Zea mays, Oryza sativa, Brachypodium distachyon, Arabidopsis thaliana, Selaginella moellendorffii, and Physcomitrella patens (Supplementary Fig. S5), indicating that DW1 is widely conserved among land plants. The amino acid alignment demonstrates that there are several conserved domains in these proteins (Supplementary Fig. S6), although none of them have been characterized. In the sorghum genome, there are two homologous genes, Sobic.004G349900 and Sobic.002G138500, which the phylogenetic analysis categorized into a different sub-group of Sobic.009g229800 (Supplementary Fig. S5), suggesting that there are at least two sub-groups of this protein family. As Sobic.009g229800 is the sole member of Sub-group 1 in sorghum, its mutation may induce the dwarf phenotype even if its homologs in sub-group 2 still function.
We examined the biological functions of the putative orthologs to sorghum Dw1, Rice transgenic lines carrying an RNAi construct that targeted the overlapping sequences of Os01g0103800 and Os03g0270700 showed semi-dwarf phenotypes with reduced internodes (Fig. 3a–c). Furthermore, the two T-DNA insertion lines of At1g76660 showed reduced plant heights in comparison with the background plants (Fig. 3d–f). These results confirm the above hypothesis that defects in the Dw1 orthologs of rice and Arabidopsis cause semi-dwarfism in these plants.
Importance of dw1 and selection in sorghum breeding
Next, we studied how widely the dw1 mutation was used in the breeding of sorghum. There are many SNPs between SIL-05 and bmr-6 within the candidate region of qCL-9. We randomly selected seven SNPs, including the causal SNP of dw1 from 57.208 Mb to 57.273 Mb (Fig. 2a). When we examined the genotypes of these SNPs in ‘Tall White Sooner Milo’ (Dw1) and ‘Dwarf White Milo’ (dw1) (Table 2), the SNPs of ‘Dwarf White Milo’ were identical to those of bmr-6, suggesting that the causal SNP of dw1 might be used in the selection process of ‘Dwarf White Milo’. In fact, the 18 kb sequence of the candidate region from ‘Dwarf White Milo’ was completely the same in bmr-6 and in BTx623 (Supplementary Fig. S4).
Interactions between Dw1 and Dw3
To compare the effects of dw1 and/or dw3 on dwarfism, we produced two NILs, dw3 alone (NIL-dw3) and the double mutant (NIL-dw1dw3) in addition to NIL-dw1 (see above), by the introgression of these mutations into SIL-05 (Supplementary Fig. S7). Interestingly, both NIL-dw1 and NIL-dw3 showed a similar elongation pattern, except at the upper (I) and lower (XII) internodes, where dw3 did not affect the upper internodes but had a greater effect than dw1 at the lower internodes (Fig. 4a, b). More importantly, dw1 and dw3 synergistically function to reduce the internode length, especially in the middle internodes IV–VII. We also performed a histological analysis on these internodes. The longitudinal cell length of the parenchyma was almost the same among the four NILs, while the number of cell per internode was significantly reduced in NIL-dw1 and NIL-dw3, and especially in NIL-dw1dw3 (Fig. 4c–h). These observations suggest that the mutations of dw1 and dw3 decreased the cell proliferation rates, whereas the double mutation had a synergistic effect on the cell proliferation rate.
Semi-dwarfism is tightly associated with lodging resistance and mechanized harvesting, which are important factors in determining crop productivity. To date, some important genes involved in growth processes have been identified to elucidate their mechanisms and applications for molecular breeding. For example, mutations of semi-dwarf1 (sd1) in rice19,23 and Reduced height (Rht) in wheat20, which encode a GA biosynthesis enzyme and a dominant suppressor protein of GA signal transduction, respectively, have been widely used to improve lodging resistance in these crops, resulting in the success of the “green revolution”. It has also been reported that the semi-dwarfism of barley, sdw1/denso, widely introgressed into cultivars grown in Europe, probably results from a defect in an ortholog of rice SD124,25,26. That GA-related mutations have been used for improving lodging resistance in rice, wheat, and barley, demonstrates the importance of decreasing the GA level, or sensitivity to GA, in reducing plant heights. However, in the case of sorghum, such strategies may not be applicable because they induce culm bending, which inevitably causes abnormal plant architecture18.
In this study, we isolated a causal gene for the semi-dwarfism of sorghum, which has been widely introgressed into many cultivars, especially those of grain sorghum, using a QTL analysis and positional cloning. We concluded that Dw1 corresponds to Sobic.009g229800 due to the following: First, within the candidate region, there is only one gene, Sobic.009g229800, which is expressed in elongating internodes and that encodes different amino acid sequences between the parental cultivars, due to a premature stop codon in the bmr-6 gene (Fig. 2a,b). Second, although there are four amino acid differences in Sobic.009g230200 between the parental cultivars, its expression was hardly detected in elongating internodes by RT-PCR or RNA-seq (Fig. 2a,b). Third, ‘Dwarf White Milo’ (dw1) also contains a premature stop codon identical to bmr-6 in Sobic.009g229800, whereas ‘Tall White Sooner Milo’ (Dw1) does not, while the genome sequences of Sobic.009g230200 are identical in the two cultivars. Fourth, knockdown or T-DNA insertion lines of orthologous genes in rice and Arabidopsis showed semi-dwarfism similar to that of the NIL-dw1 sorghum (Supplementary Fig. S7; Fig. 3). Fifth, the complementation experiment showed that transformation of Dw1 gene rescued dwarfing phenotype of the cultivar Tx430 having dw1.
How did the dw1 mutation appear and spread? We also examined the genotypes of these SNPs in 3- and 4-dwarf (meaning that they carry three or four dwarf genes) cultivars, and confirmed that these cultivars contained the identical genome segment to ‘Dwarf White Milo’ carrying the causal SNP of dw1 (Table 2), whereas, non-dwarf Kafir, Hegari, Broomcorn, and some other non-dwarf cultivars contained the same genome segment as SIL-05 (Table 2). These results coincide with the classic description that dw1 mutation occurred only once in Memphis in 1905, which resulted in the appearance of Dwarf White Milo12. Subsequently, dw1 on the genomic segment of Dwarf White Milo should have spread into various cultivars. It should be noted that the 4-dwarf Kafir also contains it in the Kafir genomic background. This demonstrates that the dw1 mutation has been widely used not only for Milo, but also for Kafir cultivars. These results suggest this single allele has been important, precious, and valuable for the lodging resistance and mechanical harvesting during the history of sorghum breeding.
A synergistic interaction was observed between DW1 and DW3, which is involved in auxin transport. The pyramiding of dwarf genes should be useful in reducing sorghum to a suitable height, because sorghum is originally a tall plant that requires a significant height reduction without inducing detrimental side effects. In this situation, it might have been important to combine a few semi-dwarfing genes, which have no effects on any other traits but have synergistic or additive effects on height. The comparative study of the internode elongation pattern among NIL-dw1, NIL-dw3, and NIL-dw1dw3 plants revealed that the effects of dw1 and dw3 are synergetic especially in the middle internodes IV–VII. (Fig. 4). Considering that many modern sorghum dwarf cultivars contain mutations in both Dw1 and Dw3, the two genes have been selected in modern breeding as one of the ideal combinations. In this context, although DW1 and DW3 are interacting during the process of cell proliferation in the internode, they might function in two different molecular mechanisms or pathways. The finding of this novel factor, DW1 might be a good bridge to study not only breeding but also the interaction of auxin and dw1 -mediated mechanisms for cell proliferation in the internode.
Plant materials and growth conditions
The F2 plants of sorghum were grown under natural field conditions at the Faculty of Agriculture Education and Research Center of Alpine Field Science of Shinshu University (Ina, Japan) in 2008. Seeds were directly sown, and established one plant per hill with an 8 cm inter-hill spacing and 75 cm inter-row spacing. HIF plants were grown at the Togo Field Science and Education Center of Nagoya University (Aichi, Japan), and in the experimental field in Okinawa (Ohgimi, Japan) in 2010 and 2011. Seeds were sown in a nursery bed in a greenhouse, and 4-week-old seedlings were transplanted to the field with 15 cm spacing between each plant in two rows (30 cm in spacing) per hill (100 cm in width). The furrow was 80 cm in width. Genomic DNA was isolated from leaves of ~6-week-old field-grown plants using a cetyltrimethyl ammonium bromide extraction protocol27 with minor modifications. The SALK T-DNA insertion lines (SALK_038235 and SALK_059180) of Arabidopsis were obtained from the Arabidopsis Biological Resource Center (Columbus, OH, USA). Arabidopsis plants for mutants and WT were cultivated in a temperature-controlled chamber at 22 °C under continuous light (for plant height analysis).
DNA simple-sequence repeat (SSR) markers
The SSR markers for sorghum, as reported by Brown et al.28, Bhattramakki et al.29, Kong et al.30, and Yonemaru et al.31 were screened, and SSR markers showing polymorphisms between the two parents were chosen. One SSR marker, SbYUC7, was additionally developed on chromosome 6. Thus, 162 SSR markers were used in this analysis.
A F2 population consisting of 185 individual plants derived from a cross between bmr-6 (a 3-dwarf short cultivar)32 and SIL-05 (a Japanese tall cultivar) was used for the distribution analysis of CL, and 96 plants were selected for the QTL analysis. The CL was measured from the ground to panicle node, and the flowering date was set as the number of days from sowing to flowering on the main panicle.
Linkage analyses and QTL identifications were performed using the MAPMAKER/QTL program33,34 based on genotypic data, while recombination frequencies were converted to genetic distances in centiMorgans (cM) using the Kosambi function35. The linkage map in Fig. 1 was created using Q-gene36. Primers used in this study are listed in Supplementary Table S1.
Positional cloning of Dw1
HIFs were used in the positional cloning. For the generation of HIFs, one plant of each genotype, bmr-6 homozygous, SIL-05 homozygous, and bmr-6 heterozygous on qCL-6, qCL-7, and qCL-9, respectively, was selected from the F3 population, because the progeny plants were clearly segregated. The F4 and F5 were generated using the Single Seed Descent method, with a DNA marker selected from the same genotype as the parent. Thus, the progenies (F5 and F6), which are RILs containing a heterozygous fragment of qCL-9, were used to generate HIFs.
RNA extraction, semi-quantitative RT-PCR analyses and RNA-seq
For semi-quantitative RT-PCR analysis, total RNAs were extracted from elongating internodes of sorghum at the vegetative stage (6 weeks after sowing) from SIL-05 and bmr-6 using TRIzol reagent (Invitrogen), or from leaves of transgenic rice using an RNeasy plant mini kit (Qiagen). The first-strand cDNA was synthesized using an Omniscript RT kit (Qiagen). The PCR parameters for the detection of Sobic.009g229800, Sobic.009g229900, Sobic.009g230000, Sobic.009g231000, and Sobic.009g232000. genes were 94 °C for 5 min followed by 35 cycles of 94 °C for 30 sec, 56 °C for 30 sec, and 72 °C for 30 sec. Sorghum ubiquitin (Sobic.001G311100; SbUbi) was used as the internal reference for all analyses. The parameters for SbUbi, Os01g0103800, Os03g0270700, and OsActin (Os03g0718100) genes were 27, 30, 30 and 25 cycles, respectively. The primers used for PCR are shown in Table S1. The extracted RNAs from the elongating internodes were subjected to RNA-seq experiments as described previously37. Primer sequences are listed in Supplementary Table S1.
Phylogenetic tree analysis
DW1 protein homologs were screened using a BLAST search of Phytozome v.9.1 (http://www.phytozome.net/). The phylogenetic tree analysis and amino acid alignment were carried out using CLUSTALW38 under default settings and boxshade ver. 3.21 (http://www.ch.embnet.org/software/BOX_doc.html). Maximum likelihood tree based on the JTT model39 was obtained using Mega software. Bootstrap values were obtained by 1,000 bootstrap replicates.
Agrobacterium-mediated rice transformation
To construct RNAi transgenic plants for simultaneous knockdowns of two genes, Os01g0103800 and Os03g0270700, a common sequence in the two genes was amplified using PCR and fused using fusion PCR in both the sense and antisense directions (primers are listed in Supplementary Table S1). The RNAi construction is based on a previously described method40. The constructs were introduced into A. tumefaciens strain EHA105 and used to infect callus of rice cv. Taichung 65 as described by Ozawa41. Transformed cells and plants were selected using hygromycin resistance, and regenerants were grown to maturity in pots in a greenhouse. Transgenic plants of the T0 (for CL), and T1 or T2 (for seed length) generations were used for the analysis.
Agrobacterium-mediated sorghum transformation
A binary vector with bar gene driven by maize ubiquitin promoter (ubipro), pBVubibar-Dw1 was constructed as follows. A digested 4.5 kb genomic DNA fragment containing Sobic.009g229800 of SIL-05 was ligated in BamHI/PstI site of pCAMBIA1380 (Cambia) (pBV-Dw1). Next, a PCR amplified a DNA fragment of bar gene (without promoter) with rbc terminator sequence (ter) was swapped for LUC gene (without a bar promoter region) of pUC-LUC vector42 using in-fusion HD kit (Clontech). Finally, a DNA fragment of ubipro-bar-ter was amplified by PCR using the constructed DNA as a template, and swapped for the hygromycin resistance gene of pBV-Dw1 by in-fusion HD kit. Agrobacterium-mediated transformation of sorghum cv. Tx430 was performed according to Wu et al.43 with a slight modification. Immature embryos of sorghum were inoculated with A. tumefaciens strain EHA105 harboring pBVubibar-Dw1. Following a 2 days co-cultivation, the immature embryos were transferred to a resting medium and cultured at 25 °C in the dark for 12 days. For selection transformed cells, the immature embryos were subcultured on a selection medium containing phophinothricin (PPT) at 25 °C in the dark for 4 weeks. The selection pressure was gradually increased from 5 to 15 mg l−1 PPT. Obtained PPT-resistant calluses were cultured on a regeneration medium at 25 °C under continuous illumination (ca. 35 μmol m−2s−1) for 4 weeks and regenerated plantlets were cultured on a root induction medium for further 4 weeks. Both media contained 5 mg l−1 PPT. Well-rooted plants were transplanted to soil in pots and grown to maturity in a greenhouse. For DNA analysis, total genomic DNA was extracted from leaves using the E.Z.N.A. SP Plant DNA Kit (Omega Biotek).
Culm longitudinal slices (110-μm in thickness) of the NILs were prepared using a vibrating microslicer (D.S.K DTK-3000), followed by toluidine blue staining. Photographs were taken using an Olympus stereoscopic microscope connected to a CCD camera (Olympus DP20). To quantify the length and number of the cells in parenchyma, we measured them in five cell files within a 2.1-mm long region of the ninth internode and then estimated the total number of cells in the internode.
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This work was partially supported by a grant from the Core Research for Evolutional Science and Technology (CREST) Program “Novel techniques of tailor-made breeding for energy crop improvement using high-throughput genotyping” of Japan Science and Technology Agency (JST); Grants-in-Aid from the NC-CARP project, the Ministry of Education, Science, Sports and Culture of Japan, and the Ministry of Agriculture; Forestry and Fisheries of Japan (Genomics for Agricultural Innovation), QTL5501. We thank K. Yano and T. Hosaki for handling DNA- and RNA-seq results, and Y. Tahara, S. Mizuno, T. Yamamura, and M. Yamamura for plant cultures in the field.
The authors declare no competing financial interests.
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Yamaguchi, M., Fujimoto, H., Hirano, K. et al. Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. Sci Rep 6, 28366 (2016). https://doi.org/10.1038/srep28366
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