Proanthocyanidins (PACs) have been reported to modulate multiple targets by simultaneously controlling many pivotal metabolic pathways in the liver. However, the precise mechanism of PAC action on the regulation of the genes that control hepatic metabolism remains to be clarified. Accordingly, we used a metabolomic approach combining both nuclear magnetic resonance and mass spectrometry analysis to evaluate the changes induced by different doses of grape-seed PACs in the liver of healthy rats. Here, we report that PACs significantly increased the hepatic nicotinamide adenine dinucleotide (NAD+) content in a dose-dependent manner by specifically modulating the hepatic concentrations of the major NAD+ precursors as well as the mRNA levels of the genes that encode the enzymes involved in the cellular metabolism of NAD+. Notably, Sirtuin 1 (Sirt1) gene expression was also significantly up-regulated in a dose-response pattern. The increase in both the NAD+ availability and Sirt1 mRNA levels, in turn, resulted in the hepatic activation of SIRT1, which was significantly associated with improved protection against hepatic triglyceride accumulation. Our data clearly indicates that PAC consumption could be a valid tool to enhance hepatic SIRT1 activity through the modulation of NAD+ levels.
Natural dietary polyphenols and specifically proanthocyanidins (PACs), the most structurally complex subclass of flavonoids, are bioactive food compounds that are primarily present in fruits and vegetables and exhibit many protective effects against cardiovascular disease1. In this context, our group and others have reported many healthy and beneficial effects of PACs on different metabolic syndrome-related pathologies, such as insulin resistance, dyslipidemia, obesity, hypertension and inflammation2,3,4,5,6,7. Furthermore, other studies performed at the molecular level have demonstrated that PACs could play an important role in the regulation of the transcriptional networks that control various critical metabolic processes in the liver. Specifically, PAC consumption was shown to protect the liver from lipid accumulation by reducing the expression of target lipogenic genes and up-regulating fatty acid oxidation8,9,10.
Several mechanisms by which PACs reduce these hepatic metabolic disturbances have been described, such as the direct interaction with intracellular signaling pathways11 and modulation of epigenetic factors, including both microRNAs12,13 and components of the DNA methylation machinery14. However, the actual molecular mechanisms involved in the health benefits of PAC consumption in the liver remain mostly speculative and the global mechanism of action is still largely unknown. This might be because previous studies have primarily focused on genomic or proteomic changes instead of assessing the direct changes in hepatic metabolites. Certainly, metabolomics, one of the most rapidly growing fields of contemporary science15, might be a good alternative to characterize the hepatic metabolites that are modified as a result of exogenous challenges, such as PAC consumption and provide new evidence linking the cellular pathways to the biological mechanisms.
In this context, two different technologies have the potential to discover the metabolic alterations in liver samples: nuclear magnetic resonance (NMR) and mass spectrometry (MS)16,17,18. However, to the best of our knowledge, there are no previous metabolomic studies in the literature aimed at evaluating the effect of PAC consumption on liver metabolites. Therefore, here, we performed a multiplatform approach combining both NMR and MS metabolomic analysis of the liver of healthy rats that were chronically supplemented with different doses of PACs. Because homeostasis is very robust, challenging homeostasis is more informative than static homeostatic studies19. Therefore, we aimed to quantitatively identify the alterations in hepatic metabolites in response to a fat overload challenge to further elucidate the mechanism by which PAC consumption can modulate lipid metabolism in liver. Our results revealed that PAC consumption could be relevant for improving hepatic lipid metabolism in an in vivo model by regulating the liver’s response through a metabolic increase in both cellular nicotinamide adenine dinucleotide (NAD+) availability and sirtuin activity.
Metabolomics revealed that PAC consumption robustly increased the hepatic NAD+ levels in a dose-response manner
The liver metabolic profile changes associated with PAC consumption were initially assessed only in the liver samples from animals receiving 0, 5 and 25 mg of PAC/kg body weight (bw) using an untargeted 1H-NMR-based metabolomics approach. A total of 46 spectral regions were identified and quantified from the 1H-NMR spectra acquired from liver extracts. Then, a multivariate principal components analysis (PCA) was performed on the resulting data for exploratory purposes. The PCA score plot accounted for a 57% variance of the original matrix and did not show any clear clustering trend of the data according to the PAC consumption groups (Fig. 1A). Subsequently, we used a one variable at a time 1-way ANOVA to compare the PAC consumption groups. The concentrations of fumaric acid (singlet, 2 × CH, δ = 6.5 ppm) were slightly decreased as a consequence of PAC consumption (P = 0.04 by Tukey’s HSD post-hoc test), whereas the hepatic nicotinamide (Nam) levels (singlet, H-2, δ = 8.9 ppm & doublet, H-6, δ = 8.7 ppm) were robustly increased in those animals supplemented with 25 mg of PAC/kg bw (Fig. 1B).
Next, as Nam is one of the main liver precursors of NAD+, these results were validated and complemented by quantifying the NAD+ levels with targeted analyses of the liver samples using triple-quadrupole mass spectrometry (QqQ-MS/MS). Notably, in order to further investigate the linear dose-dependent related effects of PAC consumption, additional liver samples from another group of animals receiving a higher dose of PAC (50 mg/kg bw) were also evaluated in targeted analyses. Accordingly, we confirmed that PAC consumption significantly increased the hepatic NAD+ levels in a dose-dependent manner (Fig. 2A), indicating that PAC administration could be a valid tool to boost the cellular NAD+ content in the liver. In addition, the hepatic Nam levels were clearly higher in the PAC-supplemented animals compared to the control group (Fig. 2B), as previously observed by global NMR. Then, we tested whether the increase in the NAD+ and Nam content would be paralleled by changes in the other major liver metabolites involved in the NAD+ cycle. Accordingly, the tryptophan (Trp), nicotinic acid (Na) and nicotinamide mononucleotide (NMN) concentrations were also significantly increased in animals supplemented with 50 mg of PAC/kg bw compared to the control rats (Fig. 2C). Additionally, a positive and significant relationship between the hepatic NAD+ concentrations and the levels of their major immediate precursors in the liver, Trp (rho = 0.622, P < 0.001) and Nam (rho = 0.604, P < 0.001), was also observed, suggesting that the increase in the hepatic NAD+ content is due to changes of these two NAD+ precursors. Finally, other liver intermediates and the end-products of NAD+ catabolism were also measured. However, the nicotinamide riboside (Nr), nicotinic acid mononucleotide (NaMN), nicotinic acid adenine dinucleotide (NaAD), nicotinamide n-oxide, nicotinuric acid and n-methylnicotinamide levels did not display significant differences among the groups (data not shown) or were below the limit of detection in all groups of animals.
PAC consumption induced an overexpression of the genes that encode the enzymes involved in the de novo NAD+ biosynthesis pathway
To analyze these changes further, we next evaluated whether the enhanced NAD+ levels upon PAC consumption could be derived from increased NAD+ biosynthesis. Thus, we determined the mRNA levels of the major enzymes of the NAD+ biosynthetic pathways. The first step of the de novo NAD+ biosynthesis pathway is the conversion of Trp into N-formylkynurenine through an enzymatic reaction catalyzed by tryptophan 2,3-dioxygenase (Tdo2). N-formylkynurenine is then directed to spontaneous cyclization to quinolinic acid, which is converted to NaMN through quinolinate phosphoribosyltransferase (Qprt) activity. NaMN is then transformed to NaAD by the nicotinamide mononucleotide adenylyltransferase (Nmnat) enzymes and NaAD is finally amidated to NAD+ by NAD+ synthetase 1 (Nadsyn1). Thus, we examined the effect of PAC consumption on the levels of the Tdo2, Qprt and Nadsyn1 mRNAs. Although we could not detect differences in the Tdo2 mRNA levels between the animal groups, PAC consumption significantly up-regulated the Qprt and Nadsyn1 mRNA levels in a dose-dependent manner (Fig. 3A). Notably, the increased levels of these transcripts were already significant at a dose of 5 mg of PAC/kg bw, indicating that this dose was sufficient to efficiently increase the mRNA levels of the major de novo NAD+ -biosynthetic enzymes.
Alternatively, NAD+ is also synthesized through the NAD+ salvage pathway from its precursors Na, Nam and Nr. Beginning with Na, the first step in NAD+ synthesis is catalyzed by nicotinic acid phosphoribosyltransferase 1 (Naprt1) and leads to the formation of NaMN. Similarly, Nam is converted to NMN by nicotinamide phosphoribosyltransferase (Nampt); NMN is also the product of phosphorylation of Nr by nicotinamide riboside kinase 1 (Nrk1). Both NaMN and NMN are then converted to NaAD by Nmnat, after which the NaMN-derived NaAD requires final amidation through Nadsyn1. Importantly, our results showed that PAC consumption did not modify the levels of the Nampt, Naprt1 or Nrk1 mRNAs (Fig. 3B). In fact, we could not even detect a statistically significant difference at a dose of 50 mg of PAC/kg bw compared to the control group, indicating that the higher NAD+ levels observed in the livers of the PAC-supplemented animals were not due to an increase in direct NAD+ synthesis from Nam, Na or Nr. Together, these results indicated that PAC consumption increases the cellular NAD+ content by a direct effect on de novo NAD+ biosynthesis rather than by indirectly affecting the major NAD+ salvage pathway.
PAC consumption modified the activity of the major NAD+ -consuming enzymes by simultaneously down-regulating the hepatic levels of the Parp1 and Cd38 mRNAs
As ADP-ribosylation reactions consume NAD+, we also analyzed whether the mRNA levels of the major genes involved in this process, Poly(ADP-ribose) polymerase 1 (Parp1) and Cyclic ADP-ribose hydrolases (Cd38), could also contribute to the increase in the NAD+ levels. Notably, at dose of 50 mg/kg bw, PAC consumption significantly down-regulated the Parp1 and Cd38 mRNA levels compared to the control animals (P = 0.04 and P = 0.014, respectively; Mann-Whitney test), without any effect at lower doses (Fig. 4). PARP1 and CD38 are the major NAD+ -consuming enzymes, thus the decrease in their activities may have also contributed to the increase in the hepatic NAD+ content. Indeed, there was a negative and significant correlation between the hepatic NAD+ concentrations and the Parp1 mRNA levels (rho = −0.661, P = 0.004) that was not as evident for the Cd38 mRNA values (rho = −0.457, P = 0.075).
PAC consumption robustly enhanced both the hepatic Sirt1 mRNA levels and SIRT1 activity in a dose-dependent manner
The ability of PAC to increase the intracellular NAD+ levels in vivo prompted us to test whether it could also modulate the sirtuin 1 (SIRT1) levels. PAC consumption resulted in a significant up-regulation of the Sirt1 mRNA levels in a dose-dependent manner (Fig. 5A). Moreover, we observed a positive and significant relationship between the Sirt1 mRNA levels and the hepatic NAD+ content (rho = 0.493, P = 0.03). The combination of higher NAD+ concentrations with higher Sirt1 mRNA levels provides an excellent scenario for increased SIRT1 activity. Accordingly, SIRT1 activity was increased in a dose-response manner, with a significant activation in the liver of rats treated with 25 or 50 mg PACs/kg bw (Fig. 5B).
Moreover, indirect measurements of SIRT1 activity also reinforced the actual activation of SIRT1 in liver by PACs. Considering that the deacetylation of forkhead O-box protein 1 (FOXO1) is mediated by SIRT1, we evaluated the transcriptional activation of FOXO1 by determining the mRNA levels of its target genes Superoxide dismutase 2 (Sod2) and Growth Arrest and DNA Damage (Gadd45) to confirm this hypothesis20. Accordingly, their mRNA levels were significantly up-regulated in the livers of the PAC-supplemented animals in a dose-dependent manner (Fig. 5C). Consistent with SIRT1 being a negative regulator of Mitochondrial uncoupling protein 2 (Ucp2) expression21, PAC consumption significantly decreased the Ucp2 mRNA levels in a similar dose-dependent pattern (Fig. 5C). Finally, as the altered NAD+ levels could also potentially impact other SIRT proteins, we also tested the levels of Sirt2 and Sirt3 transcripts, which act as cytosolic and mitochondrial sirtuins, respectively. However, no changes were observed in the mRNA levels of these genes with respect to control group (Fig. 5D).
PAC consumption improved the hepatic lipid content in both healthy rats and cultured cells in a SIRT1-mediated manner
Given the promising role of SIRT1 in protecting against metabolic liver diseases, we next evaluated the effects of PAC consumption on the liver fat content in animals subjected to an acute dietary fat challenge. Confirming our hypotheses, PACs significantly reduced the hepatic lipid accumulation in a dose-dependent manner (Fig. 6A) and this was associated with a significant decrease in both the hepatic cholesterol and triglyceride concentrations (Fig. 6B). Notably, the hepatic lipid concentrations were negatively and significantly related to the Sirt1 mRNA levels in the PAC-supplemented animals (rho = −0.678, P = 0.002). Moreover, when we applied a multivariate linear regression model to assess the role of the variables known to influence the hepatic lipid concentrations, the Sirt1 mRNA levels were the only variable that was significantly associated with fat accumulation in the liver, with a β coefficient of −0.896 (P = 0.003), highlighting the major role of SIRT1 in reducing the hepatic fat depots in the PAC-supplemented animals.
To further solidify our data, we also evaluated the efficacy of PACs in protecting against fat overaccumulation in an experimental in vitro model. Thus, HepG2 cells were incubated with a mixture of 0.5 mM palmitate and 30 mM glucose for 48 hours to induce fat-overloading. Then, the cells were treated with 100 mg/L PACs for the last 24 hours of incubation. Indeed, when the cultured cells were treated with PACs, the intracellular concentrations of triglycerides decreased significantly to basal levels, reversing the fat accumulation observed in the untreated cells (Fig. 7A). In addition, to confirm the specificity of SIRT1 in attenuating the triglyceride accumulation in cultured cells, the HepG2 cells were also treated with sirtinol, a SIRT1 inhibitor, for 24 hours. Notably, the protective effect of PACs on cellular triglyceride accumulation was significantly abrogated when the cells were treated with 100 μM sirtinol and the triglyceride levels did not significantly decrease after the PAC treatment (Fig. 7A). Finally, when the mRNA levels of the key genes involved in lipid metabolism were assessed by real time quantitative polymerase chain reaction (RT-qPCR), PAC treatment resulted in decreased Fatty acid synthase (Fas) and Acetyl-coA carboxylase (Acc) mRNA levels compared to the untreated cells, indicating that the delipidating effect of the PAC treatment may be mediated by limiting the capacity for de novo lipogenesis (Fig. 7B,C). In addition, when the cultured cells were treated with 100 μM sirtinol, the levels of the Fas and Acc mRNAs were again not affected by the PAC treatment, thus confirming that SIRT1 activation plays a critical role in regulating hepatic de novo lipogenesis and, consequently, fat accumulation in this experimental model.
Several molecular mechanisms have been described to account for the metabolic benefits of PAC consumption8,10,11,12,13. However, all of these mechanisms only partially account for all the physiological and biochemical effects of PACs. Therefore, in this study, we have used a combination of metabolomics and gene expression analyses to identify the potential global mechanism that could explain the diverse metabolic effects of PAC consumption. Interestingly, comparative liver metabolomics revealed that NAD+ and its metabolites are clear targets of PAC consumption. Certainly, PAC administration dose-dependently increased both the hepatic NAD+ content and mRNA levels of the genes involved in the NAD+ biosynthesis pathway, highlighting NAD+ homeostasis as potential PACs target in the livers of healthy rats.
Notably, other studies of our group indicate that an acute single dose of PACs is also able to modulate hepatic NAD+ levels22. Thus, altogether, these results reveal NAD+ homeostasis as a major target of PACs in liver.
NAD+ plays a pivotal role in cells, as it is indispensable for energy production and acts as reusable coenzyme in metabolic redox reactions. The roles of NAD+, however, have expanded beyond its role as a coenzyme, as NAD+ and its metabolites also act as degradation substrates for a wide range of enzymatic reactions that catalyze ADP-ribosylation and deacetylation of proteins23. Through these activities, NAD+ links the cellular metabolism to changes in signaling and transcriptional events. In addition, different studies have unequivocally demonstrated the ability of NAD+ to dynamically respond to physiological stimuli, such as exercise24 and caloric restriction25. Thus, the modulation of NAD+ homeostasis by PAC consumption empowers these compounds to modify a large number of metabolic processes in the liver. Furthermore, the liver exports NAD+ precursors to other organs, such as the brain and muscle, which have a lower capacity to synthesize NAD26. Thus, as several organs depend on NAD+ homeostasis in the liver, the modulation of NAD+ homeostasis by PACs could be extended as a general effect in the whole organism.
NAD+ availability is determined by the relative rates of NAD+ biosynthesis and degradation. Although NAD+ is primarily synthesized by the salvage pathway in the liver27, our results indicate that PAC consumption increased the NAD+ levels by enhancing the de novo NAD+ biosynthesis pathway. Indeed, PAC increased the flux through the de novo biosynthesis pathway by overexpressing some of the key enzymes involved in this pathway and by increasing the precursor’s levels. In fact, the hepatic levels of Trp were robustly increased in a dose-response pattern and it has been described that liver can increase the flux through the de novo pathway 40-fold when the levels of Trp or other NAD+ precursors increase27. Together, these results strongly suggest that PAC consumption fosters the use of Trp in the liver as the main precursor of NAD+, while maintaining the activity of the salvage biosynthetic pathway at basal levels (Fig. 8). Nampt expression was not altered by PACs at any dose. However, NMN levels, the product of the reaction catalyzed by Nampt, increased significantly at 50 mg of PACs, indicating that the actual Nampt activity in the liver of rats treated with PACs was sufficient to support the increased flux through the salvage pathway.
Interestingly, sirtuins use NAD+ as a co-substrate to remove acetyl moieties from lysines on histones and proteins, releasing Nam and O-acetyl-ADP-ribose. Generally, most sirtuins are activated when there is an energy deficiency and reduced carbohydrate energy sources, triggering cellular adaptations that improve metabolic efficiency. For example, SIRT1 activity increases during exercise24, caloric restriction or fasting25, all of which correlate with higher NAD+ levels, suggesting that NAD+ availability is the most important regulator of SIRT1 activity. In this study, PAC consumption increased SIRT1 activity in a dose-dependent pattern, according to the dose-response effect of PACs on the hepatic NAD+ levels. Notably, despite the levels of Nam, which is a potent inhibitor of Sirt128, increasing dose-dependently following PACs consumption, SIRT1 activity was not inhibited because NAD+ levels increased higher than Nam levels, in the way that the ratio between NAD+/Nam remains in favor of NAD+ as the dose of PACs was raised.
In addition, PACs significantly reduced the expression of other non-SIRT NAD+ -consuming enzymes in the liver, such as PARP1 and CD38. In fact, diverse lines of research indicate that the pharmacological repression of PARP1 drives an increase in the NAD+ content and activation of SIRT129,30, indicating that the competition between PARPs and SIRTs for NAD+ could be considered a metabolic determinant. Illustrating the opposing roles of both enzymes, PARP1 is required for the transcriptional co-activation of nuclear factor kappa B (NF-κB)31, while SIRT1 inhibits NF-κB activity through the deacetylation of RelA/p6532. Furthermore, SIRT1 also negatively regulates PARP1 transcription33. Thus, the results of this study indicate that PAC-mediated PARP-1 and CD38 repression could primarily contribute to SIRT1 activation.
Remarkably, PAC consumption also promoted Sirt1 expression in a dose-response pattern. Several mechanisms control Sirt1 expression, such as FOXO1, PPARα and PPARγ, among others. Together, these results indicate that the activation of SIRT1 by PACs was the result of increasing both the cellular NAD+ and SIRT1 concentrations. Therefore, PACs significantly foster SIRT1 activity. This property is not exclusive of PACs and other polyphenols, such as resveratrol, can also potentially modulate SIRT1 activity both in vitro and in vivo34,35. However, the mechanism of SIRT1 activation by resveratrol has been debated and recent studies show that resveratrol could not be a specific activator of SIRT136,37.
In mammals, the SIRT family comprises seven proteins, SIRT1-7, each one with a specific cellular localization and function. Thus, we also evaluated whether PAC consumption could modulate the expression of Sirt2 and Sirt3, which are primarily localized to the cytosol38 and the mitochondrial matrix39, respectively. However, the expression levels of Sirt2 and Sirt3 were not modified, indicating that PAC-mediated regulation of Sirt1 expression is specific and not a general effect of PACs on the SIRT family.
SIRT1 is an important modulator of hepatic lipid metabolism by enhancing fatty acid oxidation, decreasing lipogenesis and modulating cholesterol levels through the activation of 5′ AMP-activated protein kinase (AMPK) and the deacetylation of both steroid response element binding protein 1 (SREBP1) and farnesoid X receptor (FXR)40. Accordingly, our group previously demonstrated that PACs reduced triglyceridemia in wild type mice but not in FXR-null mice, revealing that FXR is an essential mediator of the hypotriglyceridemic actions of these compounds in vivo11. In the liver, PACs down-regulated the expression of the transcription factor SREBP1 and several SREBP1-target genes involved in lipogenesis in an FXR-dependent manner. In addition, in cultured mammalian cells, PAC treatment increased the transactivation activity of FXR only when its natural agonist, the chenodeoxycholic acid, was present in the media11. Therefore, we can speculate that the direct transactivation of FXR by PACs could be mediated, at least in part, by the consecutive increase in NAD+ content and SIRT1 activation, although further studies are needed to confirm this hypothesis. In accord with this role, it has been widely demonstrated that hepatocyte-specific deletion of SIRT1 induces hepatic lipid accumulation by up-regulating the expression of lipogenic genes and reducing fatty acid oxidation41,42, whereas overexpression of SIRT1 protects against hepatic lipid accumulation and inflammation in response to moderate- and high-fat diets43. Interestingly, our results showed that PAC administration dose-dependently attenuated the hepatic lipid accumulation induced by an acute dietary fat challenge, due to the increased NAD+ levels and SIRT1 activity. Furthermore, we demonstrated that when the HepG2 cells were treated with an SIRT1 inhibitor, the triglyceride levels did not significantly decrease after the PAC treatment. Together, these results strongly suggest that PACs prevented the hepatic lipid accumulation through a SIRT1-mediated mechanism.
In conclusion, our current study provides the first in vivo evidence that the intracellular NAD+ levels, SIRT1 activity and lipid content in liver are globally modulated in a dose-dependent manner by chronic dietary supplementation with PACs. In view of these results, PACs activated Sirt1 by both i) promoting NAD+ synthesis and ii) overexpressing Sirt1. These results suggest that PAC consumption could be a new valid strategy to enhance SIRT1 activity through the modulation of the Parp1, Cd38 and Sirt1 mRNA levels (major NAD+ -consuming enzymes) and the activation of the de novo NAD+ biosynthesis pathway. Interestingly, the PAC doses used in this experiment in rats are doses achievable in a normal human diet. Using a translation of animal to human doses44 and estimating the daily intake for a 70 kg human, the doses 5, 25 and 50 mg of PACs/kg of body weight correspond to an intake of 57, 284 and 560 mg of PACs/day, respectively. However, to translate these results from both rats and cells to humans, further studies are needed.
Materials and Methods
Grape seed PAC extract composition
The grape seed PAC extract was kindly provided by Les Dérives Résiniques et Terpéniques (Dax, France). According to the manufacturer, the extract is mainly composed of phenolic compounds (total content higher that 96%) with the phenolic content composed of monomers or flavan-3-ols (21.3%), dimers (17.4%), trimers (16.3%), tetramers (13.3%) and oligomers (5–13 units; 31.7%) of procyanidins. The phenolic composition of this extract was further analyzed by Quiñones, M. et al.45. Briefly, the analysis showed that the most important phenolic compounds contained in the extract were: catechin (58 μmol/g), epicatechin (52 μmol/g), epigallocatechin (5.50 μmol/g), epicatechin gallate (89 μmol/g), epigallocatechin gallate (1.40 μmol/g), dimeric procyanidins (250 μmol/g), trimeric procyanidins (1568 μmol/g), tetrameric procyanidins (8.8 μmol/g), pentameric procyanidins (0.73 μmol/g) and hexameric procyanidins (0.38 μmol/g).
The investigation has been conducted in accordance with the ethical standards and according to the Declaration of Helsinki and has been approved by the Ethics Review Committee for Animal Experimentation of the Universitat Rovira i Virgili. Forty six-week-old male Wistar rats were purchased from Charles River Laboratories (Barcelona, Spain). The animals were singly housed in a 12 h light-dark-cycle at 22 °C and were provided a standard chow diet (Panlab 04, Barcelona, Spain) and tap water ad libitum. After one week of adaptation, the animals were randomly divided into four groups (n = 10) and supplemented with 0 (control group), 5, 25 or 50 mg of PACs/kg body weight (bw) for 21 days. The grape seed PAC extract was dissolved in 750 μL of commercial sweetened skim condensed milk (Nestle; 100 g: 8.9 g protein, 0.4 g fat, 60.5 g carbohydrates, 1175 kJ) at the appropriate concentrations. Before supplementation, all of the rats were trained to voluntarily lick the milk and all groups were administered with the same volume of condensed milk for 21 days. The treatment was administered every day at 9:00 am. After 21 days of supplementation, the rats were fasted overnight. At 9:00 am, the rats were orally gavaged with lard oil (2.5 mL/kg bw) with the appropriate dose of the extract (0, 5, 25 or 50 mg/kg bw). Three hours later, the rats were sedated using a combination of ketamine (70 mg/kg bw, Parke-Davis, Grupo Pfizer, Madrid, Spain) and xylazine (5 mg/kg bw, Bayer, Barcelona, Spain). After anesthetization, the rats were exsanguinated from the abdominal aorta. The liver was excised and frozen immediately in liquid nitrogen and stored at −80 °C until the RNA and metabolites were extracted.
The liver metabolite extraction was performed according to the procedure described by Vinaixa M et al.16. Briefly, 50 mg of hepatic tissue was removed, flash-frozen and mechanically homogenized in 1 mL of H2O/CH3CN (1/1). The homogenates were centrifuged at 5,000 × g for 15 min at 4 °C. The supernatants (hydrophilic metabolites) were separately lyophilized overnight and stored at −80 °C until further analysis. For the NMR measurements, the hydrophilic dried extracts were reconstituted in 600 μL of D2O containing 0.67 mM trimethylsilyl propionic acid (TSP). The samples were then vortexed, homogenized for 20 min and centrifuged for 15 min at 6,000 × g at 4 °C. Finally, the redissolved samples were placed into 5 mm NMR tubes. The 1H-NMR spectra were recorded at 300 K on an Avance III 600 spectrometer (Bruker, Germany) operating at a proton frequency of 600.20 MHz using a 5 mm CPTCI triple resonance (1H, 13C, 31P) gradient cryoprobe. The one-dimensional 1H pulse experiments were performed using the nuclear Overhauser effect spectroscopy (NOESY) presaturation sequence (RD–90°–t1–90°–tm–90° ACQ) to suppress the residual water peak. Solvent presaturation with an irradiation power of 75 Hz was applied during the recycling delay (RD = 5 seconds) and mixing time (tm = 100 ms). The 90° pulse length was calibrated for each sample and varied from 6.57 to 6.99 ms. The spectral width was 12 kHz (20 ppm) and a total of 256 transients were collected into 64 k data points for each 1H spectrum. The exponential line broadening applied before Fourier transformation was 0.3 Hz. The frequency domain spectra were phased and baseline-corrected using TopSpin software (version 2.1, Bruker).
NAD+ and its related metabolites and end-products, Nam, NMN, Na, Nr, NaMN, NaAD, nicotinamide n-oxide, nicotinuric acid, n-methylnicotinamide and Trp, were measured in the liver samples using an LC-QqQ-MS/MS system consisting of an Agilent HPLC 1200 Series (Agilent Technologies, Palo Alto, USA). Briefly, 60 mg of the lyophilized liver samples were vigorously vortexed in 0.5 mL of physiological saline for 30 seconds, followed by 30 seconds of ultrasonication in a Vibra Cell (Sonics, Newton, USA). Then, 0.5 mL of acetone was added and the samples were centrifuged at 10,000 × g for 15 min at 20 °C. This procedure was repeated twice; the upper phases were mixed and evaporated under nitrogen flow to dryness. Finally, the dried samples were dissolved in 100 μL of the mobile phase at the initial conditions and injected into the liquid chromatograph (LC). A Scherzo SM-C18 (3 μm; 150 mm × 2 mm i.d.; Imtakt, Japan) was used to perform the analysis. The LC was coupled to a triple quadrupole (QqQ) mass spectrometer (MS) 6410 (Agilent Technologies, Palo Alto, USA).
Cell culture and treatments
The HepG2 human hepatoma cells obtained from the American Type Culture Collection (ECACC code 85011430) were cultured in DMEM containing 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Hepes) buffer, 10% (vol/vol) foetal bovine serum (FBS), 2 mM l-glutamine, 0.1 mM non-essential amino acids (NEAA), 100 U/mL penicillin and 100 mg/mL streptomycin, 2.5 mg/L Amphotericin B solution at 37 °C in a humidified atmosphere containing 5% CO2, with medium changes three times a week. The cells were incubated in 12-well plates at a density of 5 × 106 cells/well for 48 hours or until the cells were 70% confluent before starting the experimental treatments. To establish a hepatocellular model of fat-overloading, the HepG2 cells were incubated with a mixture of 0.5 mM palmitate (P9767, Sigma-Aldrich, Madrid, Spain) combined with 0.5% fatty acid free-BSA (A3803, Sigma-Aldrich) and 30 mM glucose (131341.1211, Panreac, Barcelona, Spain) for 48 hours. For the last 24 hours of incubation, the cells were treated with 100 mg/L PACs in the presence or absence of 10 or 100 μM sirtinol (10523, Cayman Chemical, Tallin, Estonia), a SIRT1 inhibitor. The treatments were carried out in triplicate in three independent experiments. Finally, the cell lysates were collected and stored at −80 °C for the triglyceride and RT-qPCR analyses.
Cytotoxicity of grape seed PACs on HepG2 cell line was evaluated previously and doses below 350 mg/L were safe46. Therefore, 100 mg PACs /L allows to do in vitro experiments with high levels of PACs without affecting HepG2 cell viability. Additionally, we have used this dose of PACs in many other studies, showing that this dose is effective at modulating gene expression and metabolism in HepG247,48,49. Based on the composition of the extract, the mean molecular weight of grape seed PACs extract used in this experiment is 780 g/mol; thus, 100 mg PACs /L is equivalent to 0.13 μM.
The total RNA was extracted from the liver and cultured cells using TRIzol reagent and an RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocols. The quantity and purity of RNA was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific). Samples with the RNA concentration (A260/A280 ≥ 1.8 ng/μl) and purity (A230/A260 ≥ 2.0 ng/μl) were selected. Additionally, RNA integrity was assessed by denaturing gel electrophoresis stained with SYBR Green dye (BioRad). Complementary DNAs were generated using the High-Capacity complementary DNA Reverse Transcription Kit from Life Technologies (Uppsala, Sweden). The relative mRNA expression levels of the selected genes were assessed in the liver and normalized to the cyclophilin peptidylprolyl isomerase A (Ppia) mRNA levels. The forward (F) and reverse (R) primers used in this study can be found as Supplementary Table S1 online. Briefly, a total of 10 ng of the cDNAs was subjected to quantitative RT-PCR amplification using the SYBR Green PCR Master Mix from Bio-Rad (Barcelona, Spain). The reactions were run on a CFX96 real-time system-C1000 Touch Thermal Cycler (Bio-Rad); the thermal profile settings were 50 °C for 2 min, 95 °C for 2 min and then 40 cycles at 95 °C for 15 s and 60 °C for 2 min. Finally, the fold change in the mRNA levels was calculated and normalized to the linear form by the 2−∆∆Ct calculation50. For that, only samples with a quantification cycle lower than 30 were used for fold change calculation.
SIRT1 activity assay
Hepatic SIRT1 activity was determined according to the method described by Becatti, M. et al.51 with some modifications. Liver extracts were obtained using a mild lysis buffer (50 mM Tris-HCl pH 8, 125 mM NaCl, 1 mM DTT, 5 mM MgCl2, 1 mM EDTA, 10% glycerol and 0.1% NP40). SIRT1 activity was measured using a SIRT1 direct fluorescent screening assay kit (Cayman, Ann Arbor, MI), following the manufacturer’s protocol. Briefly, a total of 25 μl of assay buffer (50 mM Tris–HCl, pH 8.0, containing 137 mM NaCl, 2.7 mM Kcl and 1 mM MgCl2), 5 μl of liver extract and 15 μl of substrate (Arg-His-Lys-Lys(ɛ-acetyl)-)-7-amino-4-methylcoumarin) solution were added to all wells. The fluorescence intensity was monitored every 2 min for 1 h using the fluorescence plate reader Bio-Tek FLx800, applying an excitation wavelength of 355 nm and an emission wavelength of 460 nm. The results are expressed as the rate of reaction for the first 30 min, when there was a linear correlation between the fluorescence and this period of time.
The liver lipids (0.5 g) were extracted using the Folch method52. An aliquot of the extract was subjected to gravimetric analysis to measure the total lipid concentrations. The remaining extract was allowed to evaporate under a nitrogen flow and dissolved in 1 mL of lipoprotein lipase buffer, containing 1,4-Piperazinediethanesulfonic acid disodium salt ((P3768, Sigma-Aldrich), MgCl2·6 H2O (M9272, Sigma-Aldrich), albumin-free fatty acids (A8806, Sigma-Aldrich) and 0.1% sodium dodecyl sulfate (L3771, Sigma-Aldrich). The triglycerides and cholesterol concentrations in the dissolved extract and in the cultured cell lysates were measured using QCA enzymatic colorimetric kits (QCA, Barcelona, Spain) according to the manufacturer’s protocols.
Data analysis and statistical methods
The pure compound references in BBioref AMIX (Bruker), HMDB and Chenomx databases were used for the 1H NMR metabolite identification. In addition, we assigned the metabolites by 1H–1H homonuclear correlation (COSY and TOCSY) and 1H–13C heteronuclear (HSQC) 2D NMR experiments and by correlation with the pure compounds run in-house. After baseline correction, the specific 1H NMR regions identified in the spectra were integrated using the AMIX 3.9 software package (Bruker, GmBH) and the resulting dataset was explored through principal component analysis (PCA). LC-QqQ-MS/MS data acquisition was performed using Masshunter software. The analyses were performed in positive electrospray ionization (ESI+) mode. The selected reaction monitoring (SRM) transitions and the individual fragmentor voltage and collision energy for each compound were evaluated using commercial standards to obtain the best instrumental conditions. Two transitions were acquired for each compound: one for quantification and a second for confirmation purposes (data not shown). The quality parameters of the analytical method (linearity, recovery, accuracy, reproducibility, limit of detection and limit of quantification) were evaluated to confirm the reliability of the method. These parameters were determined by spiking the basal liver extract with known concentrations of the standards. All of the reported values are expressed as the means ± SE (standard error) and were analyzed using the IBM SPSS for Windows statistical package (v.21.0). The differences between groups were initially calculated using ANOVA (single-factor or two factors) and the Mann–Whitney U-test; Student’s unpaired t test and Fisher’s exact test were used as necessary. Spearman’s correlation coefficients were also used to evaluate the degree of association between variables. Finally, a multiple linear regression model was fitted to evaluate which gene expression and metabolomic variables were independently associated with the hepatic fat content. The P-values for the gene expression analyses were calculated using the 2−∆∆Ct method. P-values < 0.05 were considered statistically significant.
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The authors gratefully acknowledge Yaiza Tobajas for her excellent technical assistance. This work was financially supported by grant AGL2013-40707-R from the Spanish Ministry of Economy and Competitiveness.
The authors declare no competing financial interests.
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Aragonès, G., Suárez, M., Ardid-Ruiz, A. et al. Dietary proanthocyanidins boost hepatic NAD+ metabolism and SIRT1 expression and activity in a dose-dependent manner in healthy rats. Sci Rep 6, 24977 (2016). https://doi.org/10.1038/srep24977
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