Carbonate-based Janus micromotors moving in ultra-light acidic environment generated by HeLa cells in situ

Abstract

Novel approaches to develop naturally-induced drug delivery in tumor environments in a deterministic and controlled manner have become of growing interest in recent years. Different polymeric-based microstructures and other biocompatible substances have been studied taking advantage of lactic acidosis phenomena in tumor cells, which decrease the tumor extracellular pH down to 6.8. Micromotors have recently demonstrated a high performance in living systems, revealing autonomous movement in the acidic environment of the stomach or moving inside living cells by using acoustic waves, opening the doors for implementation of such smart microengines into living entities. The need to develop biocompatible motors which are driven by natural fuel sources inherently created in biological systems has thus become of crucial importance. As a proof of principle, we here demonstrate calcium carbonate Janus particles moving in extremely light acidic environments (pH 6.5), whose motion is induced in conditioned acidic medium generated by HeLa cells in situ. Our system not only obviates the need for an external fuel, but also presents a selective activation of the micromotors which promotes their motion and consequent dissolution in presence of a quickly propagating cell source (i.e. tumor cells), therefore inspiring new micromotor configurations for potential drug delivery systems.

Introduction

The development of autonomous nano- and micromotors has been of growing interest due to potential applications in both biomedical1 and environmental fields2, starting from the well-known catalytic motors to the ones driven by an external source (e.g. magnetic field, light, electric field, ultrasonic acoustic waves)3. Studies of the initial micromotor configurations, mainly based on microstructures whose catalytic platinum surface is activated in presence of hydrogen peroxide (H2O2), have led to a better understanding of motion principles at low Reynolds numbers and the feasibility of using such entities in complex natural environments4,5,6,7,8. Major steps forward in the field of catalytic micromotors partly rely on the fine tuning of the motors’ movement not only by using a magnetic field9,10,11, but also by employing light illumination12 or thermal effects13,14 to start and stop the engines. Alternative approaches focused on using external energy sources to promote fuel-free operation of the motors, such as acoustic15,16,17 or magnetic18,19,20 energy, leading to remotely controlled non-invasive systems for future in vivo applications, such as microsurgery21,22. Additionally, it is worth to mention the cooperative behaviour and self-assembly of micromotors23,24, along with thermo and phototactic-related research25,26, all of key importance towards the development of complex organized systems where situations taking place in living environments are mimicked.

One of the most recent trends in the field of catalytic micromotors is focused on the development of autonomous entities which are driven by biocompatible fuels, ideally present in the same environment where the reaction is aimed to take place. Microtubular motors based on a catalytic inner zinc layer were reported to move in extremely acidic mouse stomach in an in vivo study, performing an autonomous release of the cargo on the stomach wall after its gradual dissolution in the gastric environment27. Alternative non-catalytic Ru/Au bimetallic nanomotors were recently reported to be controlled inside living cells by using acoustic waves28.

Among the potential biomedical applications where nanomaterials and nanotechnology could play a major role, imaging techniques for early cancer detection and related treatment therapies are on the priority list. Although He et al. reported the use of tubular polymer-based modified with Pt nanoparticles whose motion was related to a spontaneous photothermal effect13 and Janus nanomotors based on mesoporous silica nanoparticles with Cr/Pt metallic caps for drug release29, the future implementation of such micromotors is still hindered by requiring H2O2 as fuel (toxic to mammalian cellular function). However, a recent approach reported by Wilson et al. demonstrated the directional and autonomous movement of polymer stomatocytes loaded with platinum nanoparticles towards H2O2 secreting neutrophil cells, showing its potential as emerging carriers for drug delivery30. Regarding photothermal cancer treatment, multifunctional nanoparticles have arisen as potential artefacts to selectively enhance the efficacy of photothermal ablation therapy. Apart from the possibility to deliver both heat and drug to tumorigenic regions resulting in a more selective and effective treatment with minimal side effects, it is of special interest that they are biocompatible and biodegradable31,32.

The present work is inspired by the use of well-known effervescent systems based on carbonate materials (e.g. sodium carbonate, calcium carbonate) that permit the enhanced dissolution of the active pharmaceutical compound of interest, which is generally plagued by a low dissolution rate33. To promote the activation of such an effervescent system an acidic environment is required. Such biocompatible tablets are not only present in our daily life (i.e. Aspirin, Ibuprofene, Vitamin C), but are potentially interesting for nano and micrometric platforms for environmental and drug delivery/biosensing applications34. The easy-loading and functionalization of such carbonate based systems, along with pH-sensitive dissolution, make them the perfect carriers for cancer cell treatment.

The production of lactic acid as a by-product of anaerobic glucose metabolism in tumour tissues induces acidosis35, showing an average extracellular pH value of 6.5 and even reaching lower pH values from 5.0 to 5.5 in intracellular components (e.g. endosomes, lysosomes)36. Such pH gradients have been exploited for the conception and development of pH-responsive nanomaterials that can release bioactive compounds without any external trigger37. Carbonate systems have been of particular interest for being biodegradable, nontoxic to normal cells and present a cumulative drug release in acid-dependent systems38 along with their use as carriers to encapsulate and adsorb bioactive compounds39,40. Other interesting features have been observed for calcium carbonate particles, such as their application as micropumps by using differently charged particles as tracers to show the diffusioosmotic flows arising in an unsaturated aqueous solution41.

Carbonate-based microstructures were successfully applied for cargo loading and delivery in presence of acidosis metabolism38, encouraging us to exploit such material as biocompatible micromotors with the potential for directed drug delivery. Here we implement this concept by the synthesis and characterization of novel biocompatible and biodegradable asymmetric Janus motors, which move in the presence of extremely light acidic conditions attributed to tumorous growth (see Fig. 1c). We analyzed their motion patterns to determine the mechanism behind their movement and present their dissolution and movement in acidic environments that were produced in situ by Hela cells.

Figure 1
figure1

Morphological characterization of CaCO3 Janus particles.

SEM images of bare CaCO3 (a) and CaCO3 Janus particles, with a half of its surface covered with cobalt thin layer, along with the schematic from the CaCO3 Janus particles (b). Schematic of CaCO3 particles moving in acidic environment in situ generated by HeLa cells (c). Scale bar = 2 μm.

Results

Synthesis and characterization of calcium carbonate Janus particles (CaCO3 Janus particles)

Calcium carbonate microparticles were synthesized under sonication mixing conditions and conveniently dispersed onto glass substrates to further deposit a 0.8nm layer of cobalt. Such a cap induces structural asymmetry, a key feature for the directional motion of Janus particles. The present cap composition served for asymmetry issues, not showing any toxicity for short-term experiments. However, for long term experiments in future biomedical applications alternative cap compositions should be considered. Regarding characterization of the Janus particles, all the studies were performed before the cobalt cap was depositied onto the microstructures. The SEM study reveals a clearly porous surface of the particles and a size range of 6.64 ± 3.42 μm (see Fig. 1a). The crystalline structure of the particles was evaluated by XRD studies, where vaterite and calcite phases are identified in the corresponding XRD pattern (see Figure S2). The composition of calcium carbonate in the microparticles was obtained by SEM-EDX, showing the expected weight percentage per each element (see Figure S3), in addition to the SEM-EDX from the asymmetric CaCO3 Janus microparticles (see Figure S4).

Motion principle under controlled acidic pH conditions

Carbonate structures are generally associated to effervescent systems, which are directly related to the liberation of carbon dioxide (CO2). Indeed, the essential elements in such effervescent tablets where the in situ creation of carbon dioxide takes place are a mixture of acids (e.g. citric acid and tartaric acid), a CO2 donor, which corresponds to the carbonate structure (e.g. sodium bicarbonate, calcium carbonate) and the active additive (e.g. analgesics, antibiotics). All ingredients undergo a process of wet granulation, dry granulation and fusion, which is further moistened with water to obtain the effective dissolution of the drug, rapidly disintegrating and quickly releasing the active additive in less than 12 seconds. However, in our specific application, the motion principle in tumor cell conditioned acidic environment showed no bubble generation. This perfectly suited our further experimental conditions, as our interest was not focused on massively produced bubble bursts as they would not be recommendable for future in vivo applications.

Additionally, the scope of our study was taking advantage of the acidic conditions arising from acidosis metabolism. Both conditions, the lack of massive bubble production and working in light acidic environments, defined our experimental studies. Therefore, they were held in presence of a well-known and very low acidic concentration range, as to come close to the final acidic conditions naturally induced by Hela cells. CaCO3 Janus particles were tested in parafilm platforms as to avoid fluxes during the experiments in presence of a 2.5:1.25 nM Mannitol:dihydrogen citrate solution which leads to controlled acidic conditions (see Movie S1). This combination of acids is reportedly used at higher concentration for the effervescent formulation of ibuprofen42. The pH value of the resulting solution was 4.6, which was enough to induce a non-Brownian motion of CaCO3 Janus particles associated with a controlled dissolution of the CaCO3 structure. An exponential decay of the particles’ diameter was clearly observed during motion (see Fig. 2). Different concentrations were tested in the nanomolar range, showing that the higher the acid concentration was, the faster its dissolution and therefore its related motion was (see SI Fig. 1), reaching velocities of 1.8 μm/s.

Figure 2
figure2

Dissolution trend during CaCO3 Janus particles motion.

Size vs. time of CaCO3 particles at a Mannitol:Sodium dihydrogen citrate 2.5·10−9 : 1.25·10−9 M concentration. Scale bar, 25 μm.

Although this speed is not comparable to other Pt-based catalytic motors, it should be noted that here we report by far the lowest acidic concentration (2.5:1.25 nM Mannitol:dihydrogen citrate solution compared to the reported 0.1 M in PANI/Zn micromotors43) capable of driving a single micromotor. Viability studies in controlled acidic conditions for both, HeLa cells and Fibroblasts, revealed that neither HeLa cells nor Fibroblasts were affected in cell survival by the presence of the CaCO3 Janus particles or their ions after being dissolved (see Figure S6).

The key features associated with the motion of the CaCO3 Janus particles are the break of symmetry (conferred by the cobalt cap) and the local chemical gradients created by the controlled decomposition of the calcium carbonate in presence of acids, giving rise to Ca2+, HCO3 (which evolve to CO2), OH and H+ ions (see SI Movie 2). While Sen et al. described calcium carbonate particles as potential micropumps by taking advantage of the diffusioosmosis processes in unsaturated solutions related to different diffusion coefficients of the generated ions41, we here use diffusioosmotic flows to induce the motion of our asymmetric calcium carbonate Janus microparticles in acidic pH conditions.

Tumour cell conditioned acidic environment as potential fuel for micromotors

Once the directional motion of CaCO3 Janus particles was observed at nanomolar acidic range, further studies focused on implementing conditioned tumour cell media as physiologically, or rather pathologically, present fuel. Experiments were carried out on IBIDI chips (IBIDI GmbH, Munich, Germany), conceived as ideal platforms for long-term chemotaxis studies44. Such microfluidic chips are based on two separated chambers connected with a middle channel, which permits the evaluation of the motion of the Janus particles under closed and sterile conditions in absence of any flow (see Figure S5b). We applied them to study the controlled dissolution and related motion of CaCO3 particles in conditioned HeLa cell medium in one chamber with direct control experiments in presence of non-conditioned, non-buffered cell medium in the other chamber. Therefore, one of the chambers was filled with conditioned HeLa cell media (pH 6.5), while the other was filled with non-conditioned, non-buffered cell medium (pH 7.4) as a negative control. Janus and reference particles of similar size were introduced in the middle chamber being distributed not only in the middle channel but also in each chamber (see Fig. 3). The motion of the particles was evaluated in both chambers, showing only a controlled dissolution and associated motion in the conditioned medium (see Fig. 3a and SI Movie 3). In presence of non-conditioned cell media CaCO3 Janus particles were stable (no difference in its shape or diameter was observed) even after 5 hours, only showing Brownian motion (see Fig. 3b).

Figure 3
figure3

Acidic medium vs non-buffered medium.

Optical image of CaCO3 Janus particles and fluorescent latex reference particles in presence of conditioned media (a) at time 0 and after 30 minutes (reference particles highlighted in green) and in presence of non-buffered media (b) at time 0 and after 30 minutes. Schematic of the IBIDI chip, depicting CaCO3 Janus particles and reference particles in the middle channel, which are also dispersed in the chamber filled up with conditioned media (a) and the chamber filled up with non-buffered media (b). Scale bar, 20 μm.

By working with complex cell medium, we did not expect diffusioosmotic flows to be as significant as in unsaturated acidic solutions. In order to be able to carefully evaluate the motion of Janus particles under such particular conditions, reference particles (Fluoro-Max, 10 μm green fluorescent polymer microspheres) were introduced in the system (see Movie S4). Our statistical analysis of the motors’ motion relied on the in-depth examination of nanopropulsion of particles reported by Dunderdale et al45. and were applied to both, controlled acidic conditions and the cell-conditioned media. Over short periods of time, our Janus particles revealed unidirectional propulsion with a ballistic trajectory under both acidic conditions (2.5:1.25 nM, Mannitol:dihydrogen citrate and conditioned cell media) but not in negative controls. These results are displayed in the characteristic curved mean-squared displacement vs time plot, while reference particles in the same system showed only Brownian motion (see Fig. 4). By applying the equation <ΔL>2 = 4DΔt + v2Δt2 45 to the obtained mean-squared displacement vs time plot we observed that only the data obtained in acidic conditions fits the mentioned equation, showing that the apparent diffusion constant decreases in conditioned media when compared to known acidic conditions (2.5:1.25 nM Mannitol:dihydrogen citrate solution), as expected.

Figure 4
figure4

Statistics of CaCO3 Janus particles motion.

Mean-squared displacements of particles at short interval of CaCO3 Janus particles at in acidic conditions (2.5:1.25 nM, Mannitol:dihydrogen citrate) (×track) and in conditioned cell media (□ track) and reference particle in conditioned cell media ( track). Track× and □ in Fig. 4 present the corresponding fitting equation described by Dunderdale et al.45.

Motion of CaCO3 Janus particles in tumour acidic microenvironments generated by Hela cells in situ

Once CaCO3 Janus particles showed an effective motion in conditioned cell media, the next logical step was checking their motion in an environment, where the acid producing HeLa cells were actually present in the petri dish during the experiment. This way we were able to observe that the dissolution and movement of particles happened as a consequence of the change of pH by the conversion of the initial cell media into conditioned, more acidic media in vitro. To this end, into the petri dish harbouring HeLa cells, Janus micromotors were added together with fluorescent reference polymer-based particles (see schematic in SI Figure S5C). Micromotors motion was evaluated showing a continuous and directional motion with a speed of 0.544 μm/s due to the lower acidic pH concentration in conditioned cell medium (see Movie S5 and Fig. 5). It should be noted that reference particles were conveniently used as artefacts to ensure the absence of flow during the experiment. Therefore, we were able to attribute the observed CaCO3 Janus particles motion to its controlled dissolution under the acidic conditions.

Figure 5
figure5

CaCO3 Janus particles motion in tumour acidic microenvironments.

Tracking of CaCO3 Janus particles in tumor acidic microenvironments created in situ by HeLa cells incubated in non-buffered medium. For the sake of clarity, green fluorescent polymer microspheres used as reference particles are highlighted in green and H2B-mCherry HeLa cell nuclei are highlighted in red. Real time (min) in which the optical image was taken.

Discussion

We have demonstrated the feasibility of using biocompatible micromotors moving in absence of any surfactants and external fuel addition, but in presence of fuel created in situ by HeLa cells. Our studies of CaCO3 Janus particle motors reveal dynamic, non-Brownian behaviour at nanomolar acidic concentrations of eight orders of magnitude lower than reported before43. This led us to apply them to a tumour cell system, where the acidic fuel was created in situ leading to slower, but continuous and effective motion. This simple but efficient motor configuration opens up future prospects in drug delivery, offering a unique rational on-board release in presence of acidified tumor microenvironments and several advantages associated with the motion of self-propelled entities such as chemotactic behavior30. The full potential will be unleashed in a next generation of Janus CaCO3 particles, which are downscaled to the nanometer size and loaded with drugs of interest.

Methods

CaCO3 Janus microparticles preparation

The synthesis was performed in a Elmasonic S10 ultrasonic bath (Elma Schmidbauer GmbH, Germany) by mixing at once 5 ml of CaCl2 (0.33 M) and 5 ml of Na2CO3 (0.33 M) during 15 min (both reagents were purchased from Sigma Aldrich). The mixture was rapidly centrifuged at 6000 rpm during 1.5 min and washed 5 times with deionised water, being finally redispersed in 5 ml of deionized water. As to obtain a thin monolayer of the CaCO3 particles, glass substrate was cleaned with acetone and isopropanol to be further exposed to plasma cleaner during 30 sec. Due to the increased surface hydrophilic properties of the glass substrate the drop of the dispersed particles deposited onto was homogeneously dispersed, followed by a rapid soaking of the exceeding solution from one corner of the glass. Once the glass is dry, a layer of 0.8 nm of cobalt was sputtered onto the full surface, only covering half of the CaCO3 particles and therefore obtaining the desired non-symmetric Janus particles.

Cell culture in non-buffered media

HeLa cells and NIH3T3 Fibroblasts were maintained in high glucose DMEM (SIGMA D-2429, non-pH buffered), supplemented with 10% Fetal Bovine Serum, 1% Glutamine and 1% Penicillin/Streptomycin. HeLa H2B-mCherry cells were a gift from Daniel Gerlich and showed the nucleus in red fluorescence, were kept as above but Puromycin was added to the culture medium every other day. Medium was changed every other day and cells were passaged once a week. For the particle movement in the presence of tumorous cell conditioned, acidic medium, 2 × 104 HeLa cells (tumoural) or Fibroblasts (control) were seeded to cover one half of a petri dish with 35mm diameter. The experiments were conducted within 2 days. For experiments with the conditioned medium only, cells were seeded into a 25 cm2 cell culture bottle and allowed to acidify the medium. The medium was aspirated 4–5 days later and used for experiments.

Equipment

Videos obtained in static conditions were recorded at 50 frames per second using Zeiss axioScope A1 optical microscope in bright-field, coupled with an ×40 objective. Regarding the experiments held for the evaluation of CaCO3 Janus particles motion in presence of conditioned acidic medium from tumour cells, videos were recorded at 10 frames per second using the inverted microscope Zeiss axioScope A1. In both cases, Phantom Miro eX2 high-speed camera from VisionResearch was used to record the videos in the above-mentioned conditions.

Statistics

The acquired images and particles trajectories were recorded and processed by using MTrackJ plug-in from Fiji (image processing package, distribution of ImageJ). MSD evaluation was done in R (version 3.0.2, 32 bit), where mean squared displacement (MSD(τ)) is defined as the average squared Euclidian distance a CaCO3 Janus particle has covered after a certain time interval Δt and was calculated by using the equation: MSD(τ) = <[x(t+τ)-x(t)]2 +[y(t+τ)-y(t)]2>. Further data treatment done according to the studies reported by Dunderdale et al.45. Obtained mean-squared displacement vs time difference plot is fitted to the equation <ΔL>2 = 4DΔt + v2Δt2. Corresponding equation is depicted next to the MSD curve, where y is the <ΔL>2 and x is the Δt.

Additional Information

How to cite this article: Guix, M. et al. Carbonate-based Janus micromotors moving in ultra-light acidic environment generated by HeLa cells in situ. Sci. Rep. 6, 21701; doi: 10.1038/srep21701 (2016).

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Acknowledgements

The authors thank L. Restrepo, M. Medina and S. Kumar for fruitful discussion, C. Krien for assistance in metal deposition, L. Helbig for her assistance in cell culture, H. Shakur Shahabi for XRD measurements and M. Jorgensen for SEM imaging, J. Balach for SEM-EDX measurements and C. Garzon-Coral for graphic assessment. We acknowledge the financial support by the Volkswagen Foundation (contract no. 86 362).

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M.G., A.K.M. and O.G.S. conceived the idea and designed the experiments. M.G. prepared CaCO3 Janus particles, along with the corresponding motion experiments, with the support of A.K.M. on the experiments carried out with tumour cells and conditioned cell media. M.G. and B. K. performed the corresponding statistics and data treatment of Janus particles motion. M.G., A.K.M. and O.G.S. co-wrote the paper. All the authors contributed to the discussion and revision of the present manuscript.

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Guix, M., Meyer, A., Koch, B. et al. Carbonate-based Janus micromotors moving in ultra-light acidic environment generated by HeLa cells in situ. Sci Rep 6, 21701 (2016). https://doi.org/10.1038/srep21701

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