Abstract
Caffeine is the most widely-consumed psychoactive drug in the world, but our understanding of how caffeine affects our brains is relatively incomplete. Most studies focus on effects of caffeine on adenosine receptors, but there is evidence for other, more complex mechanisms. In the fruit fly Drosophila melanogaster, which shows a robust diurnal pattern of sleep/wake activity, caffeine reduces nighttime sleep behavior independently of the one known adenosine receptor. Here, we show that dopamine is required for the wake-promoting effect of caffeine in the fly and that caffeine likely acts presynaptically to increase dopamine signaling. We identify a cluster of neurons, the paired anterior medial (PAM) cluster of dopaminergic neurons, as the ones relevant for the caffeine response. PAM neurons show increased activity following caffeine administration and promote wake when activated. Also, inhibition of these neurons abrogates sleep suppression by caffeine. While previous studies have focused on adenosine-receptor mediated mechanisms for caffeine action, we have identified a role for dopaminergic neurons in the arousal-promoting effect of caffeine.
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Introduction
Caffeine is the most widely consumed psychoactive drug in the world1. Its popularity is likely due to its ability to fight drowsiness and promote arousal. In addition, caffeine can reverse the effects of sleep deprivation on alertness and cognition, as shown in both rats and humans1,2. Despite the ubiquity of caffeine in our food and drinks, our understanding of how caffeine affects our brains and bodies is relatively incomplete.
The most extensively-studied behavioral effect of caffeine is acute locomotor stimulation, which has been attributed to antagonism of adenosine receptors. There are four subtypes of adenosine receptor and caffeine antagonizes both A2A and A1 receptors in vivo3. Which of these two receptor subtypes is responsible for the motor-stimulating effect, however, is a point of contention4,5. The effect of caffeine on sleep has been relatively less well-studied. A2A receptors have been implicated in the acute wake-promoting effect of caffeine6,7, but adenosine receptors do not have an essential role in driving baseline sleep behavior. A1 and A2A mutant mice, which should mimic receptor antagonism, have no baseline sleep defects6,8. A brain-specific deletion of the A1 receptor causes reduced slow wave brain activity following sleep deprivation, but these mice do not exhibit a change in the time spent in sleep or wake states9. In addition to adenosine receptors, caffeine has many other biological targets, including GABAA receptors, ryanodine receptors, glycine receptors and phosphodiesterases10, some of which could be relevant for its effects on sleep.
We use a potent genetic model, the fruit fly Drosophila melanogaster, to understand how caffeine promotes wakefulness. Flies have one known adenosine receptor, dAdoR, which only shows 30% sequence similarity to the human adenosine receptors at the amino terminal, but has conserved ligand-binding residues11. Surprisingly, the dAdoR null mutant responds to caffeine identically to wild type flies, suggesting that caffeine promotes wake in Drosophila via adenosine receptor-independent mechanisms12. Dopamine receptors have been implicated in the effects of caffeine in Drosophila and may also influence caffeine action in mammals through interactions with adenosine receptors13,14, but a direct involvement of dopamine has not been addressed. Because the behavioral effects of caffeine are similar between flies and humans, understanding the mode of action in the fly may elucidate novel actions of caffeine in mammals as well.
Here, we show that the wake-promoting effect of caffeine in Drosophila requires the synthesis of dopamine, a potent wake-promoting neurotransmitter. The modulation of dopaminergic signaling by caffeine likely occurs presynaptically. In addition, we identify a cluster of dopaminergic neurons, which are essential for the caffeine response. We hypothesize that caffeine promotes wake by increasing activity of these neurons.
Materials and Methods
Fly Lines
All flies were raised in vials containing fly food (194g cornmeal, 81.3 g dry yeast, 184.8 g molasses, 24 g agar, 30.5 ml of 20% Tegosept and 7 ml of propionic acid per 3 liters of food). Wild type iso31 flies15 were used as a positive control for the effects of caffeine, as they consistently display reduced nighttime sleep after consuming drug-containing food. DTH BAC and DTHFS± BAC flies are analogous to the DTH whole animal (DTHg) and hypoderm selective (DTHgFS±) rescue lines as constructed in Riemensperger et al.16, but constructed via recombineering and genomic integration utilizing endogenous DTH regulatory elements in place of GAL4/UAS binary expression tools. Details of these strains will be published elsewhere. VMATp1 mutants were a kind gift of the Krantz lab (UCLA, Los Angeles, CA). TH-Gal4 (BSC8848) flies were ordered from the Bloomington Stock Center (Bloomington, IL). The restricted dopaminergic drivers TH-C1-Gal4, TH-D1-Gal4, TH-D4-Gal4, TH-F1-Gal4, TH-F2-Gal4, TH-G1-Gal4 were all generously shared with us by the Wu lab (Johns Hopkins University, Baltimore, MD) and the other dopaminergic drivers InSite0104-Gal4 and InSite0273-Gal4 were shared by the Clandinin lab (Stanford University, Stanford, CA). UAS-Shibirets flies were a gift of the David Anderson lab (CalTech, Pasadena, CA). UAS-CaLexA flies were a gift of Dr. Jing Wang’s lab (UCSD, La Jolla, CA). UAS-dTrpA1 was a gift from L. Griffith (Brandeis University, Waltham, MA).
Behavioral Assays
To assay sleep behavior, we used the Drosophila Activity Monitoring System (DAMS 3.8, TriKinetics). 5- to 10-day-old flies were individually placed in 5 mm glass tubes containing food composed of 5% sucrose and 2% agar (sucrose/agar food). Following a 1.5-day period of acclimation in incubators kept on a 12:12 light/dark schedule at 25 °C, activity was monitored for five consecutive days. Sleep behavior was analyzed using PySolo software and sleep bouts were defined at 5 or more minutes of inactivity17. For all experiments, 10–16 flies were used per treatment group, sex and genotype. Sleep graphs depict an average of all 5 days of monitoring across all 10–16 flies. While most experiments were conducted using both male and female flies, PySolo sleep graphs for female flies are shown, except where otherwise specified.
For circadian experiments, flies were entrained in DAMS monitors for two days in 12 hour light-dark cycles and then moved to constant darkness for five days. Circadian rhythms of activity were determined using ClockLab software18.
For Shibirets temperature shift experiments, fly crosses were set and raised at 18 °C to avoid prematurely silencing the neurons. Flies were then loaded into DAMS monitors in incubators set to 12:12 light-dark cycles at a temperature of 21 °C, which is permissive for the Shibirets mutation. The next day, the temperature increased to 30 °C, the restrictive temperature, at lights-on. Sleep was assayed during three days at 30 °C and averaged across all days.
For TrpA1 temperature shift experiments, fly crosses were set and raised at 18 °C to avoid prematurely activating the neurons. Flies were then loaded into DAMS monitors in incubators set to 12:12 light-dark cycles at a temperature of 21 °C, which does not activate the TrpA1 channel. After 3 days of recording, the temperature was increased to 28 °C, which opens the TrpA1 channel. Sleep was assayed during three days at 28 °C and averaged across all days.
Drug Feeding
Caffeine (Sigma-Aldrich) was mixed into melted sucrose/agar food at a concentration of 0.5 mg/ml for all experiments except for the dose-response experiment where 0.2, 0.5 and 1 mg/ml were used. L-DOPA (Tocris) was mixed into melted sucrose/agar food at a concentration of 3 mg/ml.
Confocal Microscopy
The CaLexA tool was used to measure the activity of specific populations of neurons19. CaLexA flies express a chimeric transcription factor containing a calcium-sensitive regulatory domain of the transcription factor NFAT and the LexA DNA binding and activation domains. Increases in intracellular calcium cause this transcription factor to enter the nucleus, where it binds to and activates a GFP transgene. 5- to 10-day-old CaLexA flies were moved from vials containing fly food to vials containing either sucrose/agar food or sucrose/agar food with 0.5 mg/ml caffeine. After 24 hours, flies were anesthetized on ice and brains were dissected in 1× phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (PBS-T). Ten brains were dissected per genotype and all brains were fixed for 1 hour in 4% paraformaldehyde (PFA; Electron Microscopy Sciences). Brains were washed in PBS-T and blocked for one hour in PBS-T containing 5% normal donkey serum (NDS; Jackson ImmunoRes). Brains were incubated at 4 °C overnight in primary antibody in PBS-T with 5% NDS. CaLexA signal was labeled with 1:1000 dilution of rabbit α-GFP (Life Technologies) and neuropil was stained with a 1:1000 dilution of mouse α-nc82 (Developmental Studies Hybridoma Bank). Brains were washed three times with PBS-T and stained for 2 hours with secondary antibodies in PBS-T with 5% NDS. 1:1000 dilutions were used for α-rabbit AlexaFluor 488 (Invitrogen) and α-mouse AlexaFluor 633 (Invitrogen). Brains were washed three times in PBS-T and mounted on slides using VectaShield (Vector Laboratories, Inc.). Slides were imaged on a Leica SP5 confocal microscope with a 20× objective and 0.5 μm step size. GFP intensities were quantified post-hoc on a cell-by-cell basis from individual Z-planes using ImageJ software20 and normalized to background GFP intensity.
Results
The behavioral response to caffeine requires dopamine synthesis in Drosophila
Similarly to mammals, Drosophila experience reduced sleep following caffeine feeding. This reduction of sleep can be seen in wild type (iso3115) flies across a 24-hour light/dark cycle, but the effect is most robust and reproducible in the dark (nighttime) phase (Fig. 1A). Increasing concentrations of caffeine cause further decreases in nighttime sleep, indicating that the effect is dose-dependent (p < 0.0001 for the effect of drug concentration by 2-way ANOVA with Bonferroni multiple comparisons; Fig. 1A). The 0.5 mg/mL caffeine concentration produced the largest, most reproducible loss of sleep with no toxic effects, so this concentration was used for the rest of the experiments reported here. Chronic caffeine feeding caused sustained decreases in nighttime sleep across multiple 24-hour periods (Fig. 1D). During 5 days of activity monitoring, the effect of drug on nighttime sleep was statistically significant (p < 0.0001), while the effect of time was not (p = 0.0724) by two-way ANOVA. Using Bonferroni multiple comparisons, the caffeine-fed flies slept significantly less during each of the 5 nights than the flies fed drug-free food.
Evidence from both mammals and Drosophila suggests a role for dopamine signaling in the effect of caffeine on arousal21. However, these studies implicate dopamine receptors, which in mammals can interact with adenosine receptors and thus do not unambiguously indicate a requirement of dopamine22. We tested the requirement of dopamine in the caffeine response using a transgenic fly line deficient for neural tyrosine hydroxylase (DTH), the rate-limiting enzyme in dopamine biosynthesis. DTH, encoded by the pale (ple) gene, is required in peripheral tissue during development, resulting in late embryonic lethality of a ple null mutant23,24,25. A nervous system-specific DTH mutant was created by expressing a DTH transgene containing a double frameshift mutation (DTHFS±) in a ple null mutant background. This DTHFS± transgene produces an active form of DTH in non-nervous tissues and a truncated inactive form of the enzyme in neurons16. In previous studies, expression of the double frameshift transgene and the control transgene was under the control of an upstream activating sequence (UAS) driven by Gal4 lines16,26. In the current work, we utilized two fly lines expressing the control or double-frameshift mutant forms of DTH from a BAC, specifically a 20 kb segment of the DTH genomic sequence, which was cloned and inserted into attP sites in a ple2 mutant genetic background (Cichewicz et al. manuscript in preparation). This eliminated the need for genetic crosses to create flies containing both Gal4 and UAS transgenes to drive expression of the DTH constructs and utilizes DTH driven by endogenous regulatory elements. Importantly, the DTHFS± BAC transgene rescued the viability defect of the ple mutants, although they lacked DTH and dopamine in the adult brain, as in the case of the DTHFS± transgene expressed with the UAS-Gal4 system16. Control flies contain a wild type copy of the DTH coding sequence (DTH BAC) in a ple mutant background, rescuing expression in both the nervous system and non-neural tissue.
We measured the effect of chronic caffeine exposure on iso31, DTH BAC and DTHFS± BAC flies by concomitantly exposing these flies to caffeine-containing food and monitoring their sleep behavior for five days. As noted above, the sleep-reducing effect of caffeine was most robust and reproducible during the dark phase, so nighttime sleep was quantified for all experiments. Female iso31 flies had a dose-dependent decrease in nighttime sleep when assayed on food containing increasing concentrations of caffeine compared to drug-free food (p < 0.0001 for the effect of drug concentration by 2-way ANOVA with Bonferroni multiple comparisons; Fig. 1A). DTH BAC control flies also showed a dose-dependent decrease in nighttime sleep when exposed to increasing concentrations of caffeine in the food (p = 0.0008 by 2-way ANOVA with Bonferroni multiple comparisons, when compared to control flies) (Fig. 1B). DTHFS± BAC flies, on the other hand, were resistant to the effect of caffeine on nighttime sleep, experiencing about 650 minutes of sleep per night irrespective of drug treatment (Fig. 1C). While these figures depict data for female flies, similar results were observed for males as well (data not shown).
In addition to promoting wake, caffeine lengthens circadian period in both mammals and insects12,27. We monitored rest-activity rhythms of flies in constant conditions and found that 0.5 mg/ml caffeine lengthened the circadian period of these rhythms from 23.8 to 25 hours (p < 0.0001; 2-way ANOVA with Bonferroni multiple comparisons; Fig. 1E). Although the effect of caffeine on DTH BAC control flies was more modest, it was still significant (p = 0.001). The effect of caffeine on circadian period seemed to also require dopamine, because the DTHFS± BAC flies did not display lengthened period when monitored on caffeine-containing food (p = 0.99; Fig. 1D).
Caffeine affects dopaminergic signaling upstream of DTH
We next sought to determine if we could rescue the caffeine response by restoring dopamine to DTHFS± BAC mutants. DTH catalyzes the conversion of tyrosine to L-DOPA, which is then converted to dopamine by Dopa decarboxylase (Ddc)28,29. Despite lacking neural DTH, DTHFS± BAC flies can produce dopamine if supplied with exogenous L-DOPA. Feeding L-DOPA to iso31, DTH BAC and DTHFS± BAC flies caused a sleep decrease, consistent with an augmentation of dopamine signaling in all of these genotypes (Fig. 2A–C). Both iso31 and DTH BAC flies experienced an even more drastic sleep loss when fed both L-DOPA and caffeine together, as compared to L-DOPA alone (Fig. 2A,B). Iso31flies slept 483 minutes per night when fed L-DOPA-containing food and 362 minutes per night when fed food containing both L-DOPA and caffeine (p = 0.0009 by 2-way ANOVA with Bonferroni pairwise comparisons; Fig. 2A). DTH BAC flies slept 218 minutes per night when fed L-DOPA and 54 minutes per night when fed L-DOPA and caffeine (p < 0.0001 by 2-way ANOVA with Bonferroni pairwise comparisons; Fig. 2B). Importantly, L-DOPA feeding did not rescue the caffeine responsiveness of DTHFS± BAC flies (p = 0.99 by 2-way ANOVA with Bonferroni pairwise comparisons; Fig. 2C). Because rescue of dopamine synthesis downstream of DTH did not restore the caffeine response, caffeine likely modulates dopaminergic signaling upstream of DTH. However, we cannot exclude the possibility that L-DOPA-mediated rescue of dopamine synthesis in DTHFS± BAC flies is partial and thereby insufficient for an effect of caffeine.
Dopaminergic signaling can be disrupted not only by blocking biosynthesis, but also by blocking synaptic release. Synaptic release of dopamine relies on the transport of dopamine into synaptic vesicles by the vesicular monoamine transporter (DVMAT). Disrupting this transport with a null mutation in the DVMAT gene blocks dopamine signaling and we found that this mutation also blocked the wake-promoting effect of caffeine. DVMAT null mutants (DVMATp1) outcrossed into an iso31 genetic background30 did not show any changes in night-time sleep when treated with caffeine (p = 0.86; Fig. 3).
The PAM cluster of dopaminergic neurons is required for the response to caffeine
There are several clusters of dopaminergic neurons in the fly brain, characterized by the location of their cell bodies and the anatomical targets of axonal projections31,32,33. To identify those relevant for the caffeine response, we silenced subsets of dopaminergic neurons using restricted Gal4 drivers to express the temperature-sensitive dynamin mutant Shibirets (Shits). At the restrictive temperature, 30 °C, the targeted neurons have stalled axonal transport and synaptic signaling34. We used six restricted dopaminergic Gal4 lines created by the Wu lab and two others from the InSite collection35,36. Six of the Gal4 lines still permitted a caffeine-induced loss of nighttime sleep at 30 °C when driving Shits. One fly line, TH-F2-Gal4 > Shits, had a significant caffeine-induced sleep reduction only in males; however, there was a strong trend towards sleep loss in females as well (p = 0.0639 by ANOVA with Bonferroni multiple comparisons). One Gal4 line, InSite0273, prevented a caffeine-induced sleep decrease when driving Shits at 30 °C in both males and females (Fig. 4A,B). Therefore, this driver defines a group of dopaminergic neurons which, when silenced, block the wake-promoting effect of caffeine.
Caffeine increases activity of PAM cluster neurons
The InSite0273 driver line expresses Gal4 primarily in the Paired Anterior Medial (PAM) cluster, a group of dopaminergic neurons that projects mostly to the mushroom bodies37. We monitored the effect of caffeine on the PAM neurons using the CaLexA tool, in which neural activity-induced elevation of intracellular calcium results in long-term green fluorescent protein (GFP) reporter expression19. Representative images showed a noticeable increase in GFP fluorescence in InSite0273-Gal4 labeled cells following 24 hours of caffeine feeding (Fig. 5A,B). This increase in GFP signal was significant, both in terms of the number of cells with visible GFP expression, as well as the average GFP intensity for all of the visible cells in each brain (Fig. 5C,D). This suggests that caffeine ingestion causes increased neuronal activity in the PAM cluster neurons. The effect is not seen, however, in the PPM3 cluster, which are wake-promoting dopaminergic neurons that project to the central complex and the mushroom bodies38. The PPM3 neurons show no changes in CaLexA signal following caffeine feeding, showing that the effect of caffeine on activity does not extend to all dopaminergic or wake-promoting neuronal populations.
PAM cluster neurons promote wake
We tested the effect on sleep of activating the PAM cluster neurons. The activity of neurons can be controlled in the laboratory setting using the heat-activated cation channel TrpA1. This channel opens in response to high temperature (28 °C), depolarizing the neurons expressing it and causing sustained activity39. We expressed TrpA1 under control of the 0273-Gal4 driver and assayed behavior at high and low temperatures. At 21 °C, at which temperature the TrpA1 channel is closed, experimental flies expressing TrpA1 in PAM cluster neurons had a similar sleep profile to control flies (Fig. 6A). When the behavior was assayed at 28 °C, when the TrpA1 channel is open and neurons are activated, the experimental flies showed a marked decrease in sleep which was especially evident during the nighttime (Fig. 6B). Activating PAM cluster neurons reduces sleep in both male and female flies (Fig. 6C,D), confirming that these neurons are wake-promoting.
Discussion
Many features of human sleep are observed in Drosophila and the fruit fly has been an invaluable tool for identifying sleep regulatory mechanisms. As in humans, caffeine treatment in Drosophila increases wakefulness, lowers arousal threshold and fragments sleep12,13,40. While the arousal-promoting effects of caffeine are beneficial to humans during the day, they can be disruptive to sleep at night. Thus, it is important to identify all the mechanisms through which caffeine affects brain function to reduce nighttime sleep.
Most effects of caffeine, including promoting arousal, have been studied in the context of adenosine receptor antagonism. Caffeine can bind mammalian adenosine receptors, antagonizing A1 and A2a subtypes with equal affinity in vitro and in vivo3,41. Studies in mice have implicated adenosine signaling in caffeine-induced arousal, demonstrating that global or nucleus accumbens-restricted knockdown of A2A receptors blocks the response to caffeine6,7. These studies, however, only assayed the acute response, measuring wakefulness during a 3-hour window following a single injection of caffeine in a naïve mouse. This paradigm does not mimic a coffee-sipping human, nor does it account for sleep effects on a longer time scale.
The physiological and behavioral effects of caffeine seem to vary with chronic versus acute exposure and mode of administration42. For example, animals rapidly develop tolerance to the locomotor effects of chronic caffeine administration, but sleep reduction persists40,43. Indeed, chronic caffeine administration has very different effects and pharmacology to acute administration44. While adenosine receptor antagonism may be involved in acute behavioral changes following caffeine injection, other mechanisms may contribute to the prolonged effects of caffeine on sleep and arousal. Previously, the wake-promoting effect of chronic caffeine feeding was shown to be independent of the one known adenosine receptor in Drosophila12. This finding makes Drosophila a unique model for studying adenosine-independent mechanisms of caffeine response. In this paper, we investigate the effects of chronic caffeine exposure over the course of multiple days, focusing specifically on the role of dopaminergic signaling in the wake-promoting effect of chronic caffeine intake.
Here, we show that dopamine synthesis is required for the effect of chronic caffeine on nighttime sleep in Drosophila. We observed a strong and dose-dependent reduction of sleep upon caffeine treatment and the wake-promoting effect was observed during every night of caffeine exposure. Mutants that do not produce dopamine, however, were resistant to the wake-promoting effect of caffeine. In addition to promoting wake, caffeine lengthens the period of circadian rhythms in the bread mold Neurospora crassa45, flies12 and mice27. We demonstrate that the dopamine-deficient DTHFS± BAC flies are also resistant to caffeine-induced period lengthening. While the effect of caffeine on the DTH BAC control flies is much more modest than for iso31 flies, both genotypes show statistically significant lengthening of circadian period. The difference in magnitude of caffeine response may have to do with different expression of the endogenous DTH locus in iso31 flies versus the DTH transgene of DTH BAC flies. Dopaminergic signals are known to modulate some clock-controlled behaviors. Dopamine-deficient DTHFS± BAC flies are defective in circadian entrainment and phase shifting in response to dim light cues, although the mechanisms involved have not been identified26.
As dopamine is a potent wake-promoting neurotransmitter, a role for it in the arousal-promoting effects of caffeine is not surprising. Previous studies in Drosophila showed that the dopamine receptor is required for sleep-reducing effects of caffeine13,46,47. As in mammals (see below), this could reflect an indirect effect, where dopamine receptors interact with other postsynaptic receptors. Our data suggest that caffeine acts presynaptically in dopamine-synthesizing neurons. Indeed, we found that L-DOPA could not restore caffeine responsiveness to DTHFS± BAC flies. Importantly, L-DOPA was able to reduce sleep in these mutants, indicating that they contain both the neural circuitry and receptors by which dopamine promotes wakefulness. Lack of a response to caffeine is not due to a floor effect, as the DTH BAC control flies show a similar reduction in sleep to DTHFS± BAC flies after L-DOPA feeding and these controls show a further decrease with the addition of caffeine. However, we acknowledge that effects of L-DOPA could be restricted, either spatially or in terms of magnitude, thereby failing to restore the dopamine required for a caffeine response in DTHFS± BAC flies. We also blocked effects of caffeine on sleep by perturbing release. A presynaptic effect of caffeine was supported by our finding that mutants for the vesicular monoamine transporter (DVMAT), which cannot package neurotransmitters like dopamine into synaptic vesicles, are resistant to caffeine48.
Caffeine has been linked to dopaminergic signaling in mammals, but always through adenosine receptors, which dimerize with and inhibit dopamine receptors14. Interestingly, though, dopamine levels rise in the brains of mice after acute caffeine administration, which may reflect effects of caffeine on dopamine synthesis or release49,50. In addition, increased extracellular dopamine causes caffeine hypersensitivity, as shown in both dopamine transporter (DAT) mutant mice and DAT (fumin) mutant flies13,51. Also, humans carrying a polymorphism associated with lower expression of the dopamine transporter are hypersensitive to caffeine, suggesting conserved mechanisms52.
The Drosophila brain contains many clusters of dopaminergic neurons, based on their locations and their anatomical targets33. By acutely silencing subsets of neurons, we identified the group defined by the InSite0273-Gal4 driver line, specifically the PAM cluster of neurons, as important for caffeine-induced sleep loss37. The PAM neurons primarily project to the medial portion and the tip of the mushroom body beta and beta prime lobes53,54,55. This is consistent with the finding that the DopR dopamine receptor is required in the mushroom bodies for the caffeine response13. Interestingly, previously-defined groups of wake-promoting neurons were dispensable in the caffeine response. Th-D4-Gal4, Th-D1-Gal4 and TH-G1-Gal4, which promote wake when transiently activated35, did not block the wake-promoting effect of caffeine when silenced. Therefore, the wake-promoting circuit responsible for the caffeine response seems to be distinct from previously-identified circuits.
We confirmed, using CaLexa, that the PAM cluster neurons are indeed modulated by caffeine. The InSite0273-Gal4 line expresses in all (~100–130) neurons of the PAM cluster37. However, only between 4 and 22 showed visible activity-dependent GFP expression at baseline, which increased to between 11 and 42 when the flies were fed caffeine. This indicates that only some PAM neurons are highly active at baseline and/or are activated by caffeine. We show that the group of PAM cluster neurons expressing the 0273-Gal4 driver are wake-promoting when activated, supporting the proposition that the activation of these cells by caffeine is the mechanism by which caffeine promotes wake. Further dissection of the PAM cluster of neurons may elucidate the identity of the caffeine-activated and wake-promoting subset(s).
Interestingly, silencing cells labeled by the InSite0104-Gal4 line, which expresses in about 40 of the PAM neurons37, is not sufficient to block the caffeine response. The TH-G1-Gal4 and TH-C1-Gal4 driver lines also express in some PAM cluster neurons35, a subset which we demonstrate are dispensable for the effect of caffeine on sleep. Studies from the Waddell lab show specialized reward functions for subsets of PAM neurons, underscoring their functional heterogeneity37,56. A subset of 15 dopaminergic neurons in the PAM cluster and the mushroom body prime lobes they innervate were recently shown to be specifically required for normal performance of the flies in a startle-induced climbing response (startle-induced negative geotaxis)54 as well as for the maintenance of air-puff triggered flight57. Additional studies will be required to characterize the neurons involved in the caffeine response within the PAM cluster.
The molecular mechanism by which caffeine increases dopaminergic signaling is still unclear and how this alteration of dopaminergic signaling interacts with other caffeine-regulated pathways to contribute to behavioral outputs requires further study. The ability of caffeine to promote arousal requires PKA signaling in the brain; it is possible that caffeine activates PKA in dopaminergic neurons by inhibiting phosphodiesterases (PDEs)12,41,58. Alternatively, caffeine could stimulate dopaminergic neurons by activating ryanodine receptors, which are the major mediators of activity-induced calcium release in the cell59. Caffeine may also act on other cell surface receptors, or perhaps on an as-yet-unidentified adenosine receptor in the fly, as suggested by the fact that caffeine-induced sleep loss is mimicked by specific adenosine receptor antagonists in Drosophila13. We note though that an effect of caffeine on dopamine signaling could account for its positive effect on cognitive ability. An interesting question for future experiments is why the PAM cluster neurons are specifically involved in the caffeine effect. These neurons may express higher levels of sleep-relevant caffeine targets, or may simply be the only sleep-modulating neurons among caffeine-sensitive cells. While the direct molecular target is still unknown, we report the novel finding that caffeine promotes wake by activating a subset of dopaminergic neurons.
Additional Information
How to cite this article: Nall, A. H. et al. Caffeine promotes wakefulness via dopamine signaling in Drosophila. Sci. Rep. 6, 20938; doi: 10.1038/srep20938 (2016).
References
Penetar, D. et al. Caffeine reversal of sleep deprivation effects on alertness and mood. Psychopharmacology. 112, 359–365 (1993).
Alhaider, I. A., Aleisa, A. M., Tran, T. T., Alzoubi, K. H. & Alkadhi, K. A. Chronic Caffeine Treatment Prevents Sleep Deprivation-Induced Impairment of Cognitive Function and Synaptic Plasticity. Sleep. 33, 437–444 (2010).
Fredholm, B. B., Irenius, E., Kull, B. & Schulte, G. Comparison of the potency of adenosine as an agonist at human adenosine receptors expressed in Chinese hamster ovary cells. Biochem Pharmacol. 61, 443–448 (2001).
Snyder, S. H., Katims, J. J., Annau, Z., Bruns, R. F. & Daly, J. W. Adenosine receptors and behavioral actions of methylxanthines. Proc Natl Acad Sci USA. 78, 3260–3264 (1981).
Spealman, R. D. Psychomotor stimulant effects of methylxanthines in squirrel monkeys: relation to adenosine antagonism. Psychopharmacology (Berl) . 95, 19–24 (1988).
Huang, Z. L. et al. Adenosine A2A, but not A1, receptors mediate the arousal effect of caffeine. Nat Neurosci. 8, 858–859 (2005).
Lazarus, M. et al. Arousal effect of caffeine depends on adenosine A2A receptors in the shell of the nucleus accumbens. J Neurosci. 31, 10067–10075 (2011).
Stenberg, D. et al. Sleep and its homeostatic regulation in mice lacking the adenosine A1 receptor. J Sleep Res. 12, 283–290 (2003).
Bjorness, T. E., Kelly, C. L., Gao, T., Poffenberger, V. & Greene, R. W. Control and function of the homeostatic sleep response by adenosine A1 receptors. J Neurosci. 29, 1267–1276 (2009).
Mustard, J. A. The buzz on caffeine in invertebrates: effects on behavior and molecular mechanisms Cell Mol Life Sci. 71, 1375–1382 (2014).
Dolezelova, E., Nothacker, H. P., Civelli, O., Bryant, P. J. & Zurovec, M. A Drosophila adenosine receptor activates cAMP and calcium signaling. Insect Biochem Mol Biol. 37, 318–329 (2007).
Wu, M. N. et al. The effects of caffeine on sleep in Drosophila require PKA activity, but not the adenosine receptor. J Neuroscience . 29, 11029–11037 (2009).
Andretic, R., Kim, Y. C., Jones, F. S., Han, K. A. & Greenspan, R. J. Drosophila D1 dopamine receptor mediates caffeine-induced arousal. Proc Natl Acad Sci USA. 105, 20392–20397 (2008).
Salmi, P., Chergui, K. & Fredholm, B. B. Adenosine-dopamine interactions revealed in knockout mice. J Mol Neurosci. 26, 239–244 (2005).
Ryder, E. et al. The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics. 167, 797–813 (2004).
Riemensperger, T. et al. Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proc Natl Acad Sci USA. 108, 834–839 (2011).
Gilestro, G. F. & Cirelli, C. pySolo: a complete suite for sleep analysis in Drosophila. Bioinformatics. 25, 1466–1467 (2009).
Pfeiffenberger, C., Lear, B. C., Keegan, K. P. & Allada, R. Processing circadian data collected from the Drosophila Activity Monitoring (DAM) System. Cold Spring Harb Protoc. 2010, pdb.prot5519 (2010).
Masuyama, K., Zhang, Y., Rao, Y. & Wang, J. W. Mapping neural circuits with activity-dependent nuclear import of a transcription factor. J Neurogenet. 26, 89–102 (2012).
Abramoff, M. D., Magalhaes, P. J. & Ram S. J. Image Processing with ImageJ. Biophotonics International . 11, 36–42 (2004).
Chen, J. F. et al. What knock-out animals tell us about the effects of caffeine. J Alzheimers Dis. 20, S17–S24 (2010).
Xie, X., Ramkumar, V. & Toth, L. A. Adenosine and dopamine receptor interactions in striatum and caffeine-induced behavioral activation. Comp Med . 57, 538–545 (2007).
Jürgens, G., Wieschaus, E., Nusslein-Volhard, C. & Kluding, H. Mutations affecting the pattern of the larval cuticle in Drosophila melanogaster. II. Zygotic loci on the third chromosome. Roux Arch Dev Biol. 193, 283–295 (1984).
Neckameyer, W. S. & White, K. Drosophila tyrosine hydroxylase is encoded by the pale locus. J Neurogenet. 8, 189–199 (1993).
Friggi-Grelin, F., Iché, M. & Birman, S. Tissue-specific developmental requirements of Drosophila tyrosine hydroxylase isoforms. Genesis. 35, 260–269 (2003).
Hirsh, J. et al. Roles of dopamine in circadian rhythmicity and extreme light sensitivity of circadian entrainment. Curr Biol. 20, 209–214 (2010).
Oike, H., Kobori, M., Suzuki, T. & Ishida, N. Caffeine lengthens circadian rhythms in mice. Biochem Biophys Res Commun. 410, 654–658 (2011).
Budnik, V. & White, K. Genetic dissection of dopamine and serotonin synthesis in the nervous system of Drosophila melanogaster. J Neurogenet. 4, 309–314 (1987).
Livingstone, M. S. & Tempel, B. L. Genetic dissection of monoamine neurotransmitter synthesis in Drosophila. Nature. 303, 67–70 (1983).
Nall, A. H. & Sehgal, A. Small-molecule screen in adult Drosophila identifies VMAT as a regulator of sleep. J Neurosci. 33, 8534–8540 (2013).
Nässel, D. R. & Elekes, K. Aminergic neurons in the brain of blowflies and Drosophila: dopamine- and tyrosine hydroxylase-immunoreactive neurons and their relationship with putative histaminergic neurons. Cell Tissue Res. 267, 147–167 (1992).
Friggi-Grelin, F. et al. Targeted gene expression in Drosophila dopaminergic cells using regulatory sequences from tyrosine hydroxylase. J Neurobiol. 54, 618–627 (2003).
Mao, Z. & Davis, R. L. Eight different types of dopaminergic neurons innervate the Drosophila mushroom body neuropil: anatomical and physiological heterogeneity. Front Neural Circuits . 3, 5 (2009).
Kitamoto, T. Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. J Neurobiol. 47, 81–92 (2001).
Liu, Q., Liu, S., Kodama, L., Driscoll, M. R. & Wu, M. N. Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila. Curr Biol. 22, 2114–2123 (2012).
Gohl, D. M. et al. A versatile in vivo system for directed dissection of gene expression patterns. Nat Methods. 8, 231–237 (2011).
Burke, C. J. et al. Layered reward signalling through octopamine and dopamine in Drosophila. Nature. 492, 433–437 (2012).
Ueno, T. et al. Identification of a dopamine pathway that regulates sleep and arousal in Drosophila. Nat Neurosci. 15, 1516–1523 (2012).
Pulver, S. R., Pashkovski, S. L., Hornstein, N. J., Garrity, P. A. & Griffith, L. C. Temporal dynamics of neuronal activation by Channelrhodopsin-2 and TRPA1 determine behavioral output in Drosophila larvae. J Neurophysiol. 101, 3075–3088 (2009).
Roehrs, T. & Roth, T. Caffeine: sleep and daytime sleepiness. Sleep Med Rev. 12, 153–162 (2008).
Fredholm, B. B. & Lindström, K. Autoradiographic comparison of the potency of several structurally unrelated adenosine receptor antagonists at adenosine A1 and A(2A) receptors. Eur J Pharmacol. 380, 197–202 (1999).
Ferré, S. An update on the mechanisms of the psychostimulant effects of caffeine. J Neurochem. 105, 1067–1079 (2008).
Finn, I. B. & Holtzman, S. G. Tolerance to caffeine-induced stimulation of locomotor activity in rats. J Pharmacol Exp Ther. 238, 542–546 (1986).
Jacobson, K. A., von Lubitz, D. K., Daly, J. W. & Fredholm, B. B. Adenosine receptor ligands: differences with acute versus chronic treatment. Trends Pharmacol Sci. 17, 108–13 (1996).
Feldman, J. F. Circadian periodicity a neurospora: alteration by inhibitors of cyclic AMP phosphodiesterase. Science. 190, 789–790 (1975).
Ganguly-Fitzgerald, I., Donlea, J. & Shaw, P. J. Waking experience affects sleep need in Drosophila. Science. 313, 1775–1781 (2006).
Kume, K., Kume, S., Park, S. K., Hirsh, J. & Jackson, F. R. Dopamine is a regulator of arousal in the fruit fly. J Neurosci. 25, 7377–7384 (2005).
Simon, A. F. et al. Drosophila vesicular monoamine transporter mutants can adapt to reduced or eliminated vesicular stores of dopamine and serotonin. Genetics. 181, 525–541 (2009).
Solinas, M. et al. Caffeine induces dopamine and glutamate release in the shell of the nucleus accumbens. J Neurosci. 22, 6321–6324 (2002).
Okada, M., Mizuno, K. & Kaneko, S. Adenosine A1 and A2 receptors modulate extracellular dopamine levels in rat striatum. Neurosci Lett. 212, 53–56 (1996).
Wisor, J. P. et al. Dopaminergic role in stimulant-induced wakefulness. J Neurosci. 21, 1787–1794 (2001).
Holst, S. C. et al. Dopaminergic role in regulating neurophysiological markers of sleep homeostasis in humans. J Neurosci. 34, 566–573 (2014).
Claridge-Chang, A. et al. Writing memories with light-addressable reinforcement circuitry. Cell. 139, 405–415 (2009).
Riemensperger, T. et al. A single dopamine pathway underlies progressive locomotor deficits in a Drosophila model of Parkinson disease. Cell Rep . 5, 952–960 (2013).
Pech, U., Pooryasin, A., Birman, S. & Fiala, A. Localization of the contacts between Kenyon cells and aminergic neurons in the Drosophila melanogaster brain using SplitGFP reconstitution. J Comp Neurol. 521, 3992–4026 (2013).
Lin, S. et al. Neural correlates of water reward in thirsty Drosophila. Nat Neurosci. 17, 1536–1542 (2014).
Agrawal, T. & Hasan, G. Maturation of a central brain flight circuit in Drosophila requires Fz2/Ca(2+) signaling. eLife May 8;4. doi: 10.7554/eLife.07046 (2015).
Ribeiro, J. A. & Sebastião, A. M. Caffeine and adenosine. J Alzheimers Dis. 20, S3–S15 (2010).
McPherson, P. S. et al. The brain ryanodine receptor: a caffeine-sensitive calcium release channel. Neuron. 7, 17–25 (1991).
Acknowledgements
We would like to acknowledge our funding sources: AN was supported by NIH Grant 5 T32 HL 7953-13, SB received support from the Fondation de France (2012-00024435) and the Labex MemoLife (ANR-10-LABX-54 MEMO LIFE) and JH and KC are supported by NIH grant GM084128. AS is an Investigator of the HHMI.
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A.N. and A.S. designed experiments, A.N., I.S. and K.C. conducted experiments and analyzed data, J.H. and S.B. contributed reagents, A.N. prepared the figures, A.N. and A.S. wrote the main manuscript and other authors commented on it.
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Nall, A., Shakhmantsir, I., Cichewicz, K. et al. Caffeine promotes wakefulness via dopamine signaling in Drosophila. Sci Rep 6, 20938 (2016). https://doi.org/10.1038/srep20938
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DOI: https://doi.org/10.1038/srep20938
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